An improved method to obtain antigen-excreting Toxocara canis larvae.

Detalhes bibliográficos
Autor(a) principal: Neves, Neuza Maria Alcântara
Data de Publicação: 2008
Outros Autores: Santos, Alex Barbosa dos, Mendonça, Lívia Ribeiro, Figueiredo, Camila Alexandrina Viana, Pontes-de-Carvalho, Lain Carlos
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/8300
Resumo: Universidade Federal da Bahia. Instituto de Ciências da Saúde. Salvador, BA, Brasil
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spelling Neves, Neuza Maria AlcântaraSantos, Alex Barbosa dosMendonça, Lívia RibeiroFigueiredo, Camila Alexandrina VianaPontes-de-Carvalho, Lain Carlos2014-09-02T11:41:25Z2014-09-02T11:41:25Z2008ALCÂNTARA-NEVES, N. M. et al. An improved method to obtain antigen-excreting Toxocara canis larvae. Experimental Parasitology, v. 119, n. 3, p. 349-351, 2008.1090-2449https://www.arca.fiocruz.br/handle/icict/830010.1016/j.exppara.2008.03.006engElsevier IncAn improved method to obtain antigen-excreting Toxocara canis larvae.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal da Bahia. Instituto de Ciências da Saúde. Salvador, BA, BrasilUniversidade Federal da Bahia. Instituto de Ciências da Saúde. Salvador, BA, BrasilUniversidade Federal da Bahia. Instituto de Ciências da Saúde. Salvador, BA, BrasilUniversidade Federal da Bahia. Instituto de Ciências da Saúde. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Escola Bahiana de Medicina e Saúde Pública. Salvador, BA, BrasilToxocara canis is a dog helminth which causes visceral larva migrans (VLM) when infecting humans as a larva. The infection is demonstrated by detecting IgG antibodies against excretory–secretory larval antigens (ESLA) in serum by ELISA. The production of ESLA involves the collection of adult worms from dog puppy stools, the separation of eggs from dissected uteri, and the in vitro growing of egg-derived larvae, following the time-consuming and laborious protocol described by De Savigny [De Savigny, D.H., 1975. In vitro maintenance of T. canis larvae and a simple method for the production of Toxocara ES antigen for the uses in serodiagnostic tests for visceral larva migrans. Journal of Parasitology 61, 781–782]. In this work, an improved protocol for obtaining T. canis larvae is described. The modifications proposed improved the efficiency of the original De Savigny method in three ways: (i) increasing the parasite yield up to five fold, (ii) improving the larval purity, and (iii) markedly reducing the execution time of the protocol.ESLAExcretory–secretory larval antigensELISAEnzyme-linked immunosorbent assayToxocara canisLarva purificationDoenças do Cão/parasitologiaToxocara canis/isolamento & purificaçãoToxocaríase/parasitologiaAnimaisAntígenos de Helmintos/metabolismoCãesFezes/parasitologiaFemininoHumanosLarva Migrans Visceral/diagnósticoToxocara canis/imunologiainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://www.arca.fiocruz.br/bitstream/icict/8300/1/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD51ORIGINALAlcantara Neves NM An Improved method.......pdfAlcantara Neves NM An Improved method.......pdfapplication/pdf349925https://www.arca.fiocruz.br/bitstream/icict/8300/2/Alcantara%20Neves%20NM%20An%20Improved%20method.......pdff6433ce20f8aafbdc11ef77037e107beMD52TEXTAlcantara Neves NM An Improved method.......pdf.txtAlcantara Neves NM An Improved method.......pdf.txtExtracted texttext/plain16036https://www.arca.fiocruz.br/bitstream/icict/8300/3/Alcantara%20Neves%20NM%20An%20Improved%20method.......pdf.txt2834a0523e1ec125d7d67a87ba321dc6MD53icict/83002023-03-15 14:33:21.273oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-15T17:33:21Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv An improved method to obtain antigen-excreting Toxocara canis larvae.
title An improved method to obtain antigen-excreting Toxocara canis larvae.
spellingShingle An improved method to obtain antigen-excreting Toxocara canis larvae.
Neves, Neuza Maria Alcântara
ESLA
Excretory–secretory larval antigens
ELISA
Enzyme-linked immunosorbent assay
Toxocara canis
Larva purification
Doenças do Cão/parasitologia
Toxocara canis/isolamento & purificação
Toxocaríase/parasitologia
Animais
Antígenos de Helmintos/metabolismo
Cães
Fezes/parasitologia
Feminino
Humanos
Larva Migrans Visceral/diagnóstico
Toxocara canis/imunologia
title_short An improved method to obtain antigen-excreting Toxocara canis larvae.
title_full An improved method to obtain antigen-excreting Toxocara canis larvae.
title_fullStr An improved method to obtain antigen-excreting Toxocara canis larvae.
title_full_unstemmed An improved method to obtain antigen-excreting Toxocara canis larvae.
title_sort An improved method to obtain antigen-excreting Toxocara canis larvae.
author Neves, Neuza Maria Alcântara
author_facet Neves, Neuza Maria Alcântara
Santos, Alex Barbosa dos
Mendonça, Lívia Ribeiro
Figueiredo, Camila Alexandrina Viana
Pontes-de-Carvalho, Lain Carlos
author_role author
author2 Santos, Alex Barbosa dos
Mendonça, Lívia Ribeiro
Figueiredo, Camila Alexandrina Viana
Pontes-de-Carvalho, Lain Carlos
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Neves, Neuza Maria Alcântara
Santos, Alex Barbosa dos
Mendonça, Lívia Ribeiro
Figueiredo, Camila Alexandrina Viana
Pontes-de-Carvalho, Lain Carlos
dc.subject.en.pt_BR.fl_str_mv ESLA
Excretory–secretory larval antigens
ELISA
Enzyme-linked immunosorbent assay
Toxocara canis
Larva purification
topic ESLA
Excretory–secretory larval antigens
ELISA
Enzyme-linked immunosorbent assay
Toxocara canis
Larva purification
Doenças do Cão/parasitologia
Toxocara canis/isolamento & purificação
Toxocaríase/parasitologia
Animais
Antígenos de Helmintos/metabolismo
Cães
Fezes/parasitologia
Feminino
Humanos
Larva Migrans Visceral/diagnóstico
Toxocara canis/imunologia
dc.subject.decs.pt_BR.fl_str_mv Doenças do Cão/parasitologia
Toxocara canis/isolamento & purificação
Toxocaríase/parasitologia
Animais
Antígenos de Helmintos/metabolismo
Cães
Fezes/parasitologia
Feminino
Humanos
Larva Migrans Visceral/diagnóstico
Toxocara canis/imunologia
description Universidade Federal da Bahia. Instituto de Ciências da Saúde. Salvador, BA, Brasil
publishDate 2008
dc.date.issued.fl_str_mv 2008
dc.date.accessioned.fl_str_mv 2014-09-02T11:41:25Z
dc.date.available.fl_str_mv 2014-09-02T11:41:25Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.citation.fl_str_mv ALCÂNTARA-NEVES, N. M. et al. An improved method to obtain antigen-excreting Toxocara canis larvae. Experimental Parasitology, v. 119, n. 3, p. 349-351, 2008.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/8300
dc.identifier.issn.none.fl_str_mv 1090-2449
dc.identifier.doi.none.fl_str_mv 10.1016/j.exppara.2008.03.006
identifier_str_mv ALCÂNTARA-NEVES, N. M. et al. An improved method to obtain antigen-excreting Toxocara canis larvae. Experimental Parasitology, v. 119, n. 3, p. 349-351, 2008.
1090-2449
10.1016/j.exppara.2008.03.006
url https://www.arca.fiocruz.br/handle/icict/8300
dc.language.iso.fl_str_mv eng
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dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier Inc
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