Kinetoplastid membrane protein-11 is present in promastigotes and amastigotes of Leishmania amazonensis and its surface expression increases during metacyclogenesis
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2010 |
| Outros Autores: | , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/22875 |
Resumo: | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunoparasitologia. Rio de Janeiro, RJ. Brasil. |
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Matos, Denise C. S.Faccioli, Lanuza A. P.Finkelstein, Léa CysneLuca, Paula Mello deCorte-Real, SuzanaArmôa, Geraldo R. G.Lemes, Elezer Monte BlancoRicardo, Debora DecoteMendonça, Sergio C. F.2017-10-19T10:27:02Z2017-10-19T10:27:02Z2010MATOS, Denise C. S. et al. Kinetoplastid membrane protein-11 is present in promastigotes and amastigotes of Leishmania amazonensis and its surface expression increases during metacyclogenesis. Mem Inst Oswaldo Cruz, v.105, n.3, p.341-347, May 2010.0074-0276https://www.arca.fiocruz.br/handle/icict/22875engFundação Oswaldo Cruz. Instituto Oswaldo CruzLeishmaniosemetaciclogêneseExpressãoProteínas de MembranaLeishmania amazonensisKMP-11expressionamastigotepromastigotemetacyclogenesisKinetoplastid membrane protein-11 is present in promastigotes and amastigotes of Leishmania amazonensis and its surface expression increases during metacyclogenesisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunoparasitologia. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunoparasitologia. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunoparasitologia. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Tecnologia Recombinante. Rio de Janeiro, RJ. Brasil.Fundação Ooswaldo Cruz. Instituto de Tecnologia em Imunobiológicos. Laboratório de Tecnologia Recombinante. Rio de Janeiro, RJ, Brasil.Fundação Ooswaldo Cruz. Instituto de Tecnologia em Imunobiológicos. Laboratório de Tecnologia Recombinante. Rio de Janeiro, RJ, Brasil.Universidade Federal Rural do Rio de Janeiro. Instituto de Veterinária. Departamento de Microbiologia e Imunologia. Seropédica, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunoparasitologia. Rio de Janeiro, RJ. Brasil.Kinetoplastid membrane protein-11 (KMP-11), a protein present in all kinetoplastid protozoa, is considered a potential candidate for a leishmaniasis vaccine. A suitable leishmaniasis vaccine candidate molecule must be expressed in amastigotes, the infective stage for mammals. However, the expression of KMP-11 in Leishmania amastigotes has been a subject of controversy. We evaluated the expression of this molecule in logarithmic and stationary growth phase promastigotes, as well as in amastigotes, of Leishmania amazonensis by immunoblotting, flow cytometry and immunocytochemistry, using a monoclonal antibody against KMP-11. We found that KMP-11 is present in promastigotes and amastigotes. In both stages, the protein was found in association with membrane structures (at the cell surface, flagellar pocket and intracellular vesicles). More importantly, its surface expression is higher in amastigotes than in promastigotes and increases during metacyclogenesis. The increased expression of KMP-11 in metacyclic promastigotes, and especially in amastigotes, indicates a role for this molecule in the parasite relationship with the mammalian host. The presence of this molecule in amastigotes is consistent with the previously demonstrated immunoprotective capacity of vaccine prototypes based on the KMP-11-coding gene and the presence of humoral and cellular immune responses to KMP-11 in Leishmania-infected humans and animals.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/22875/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALsuzana_cortereal_etal_IOC_2010.pdfsuzana_cortereal_etal_IOC_2010.pdfapplication/pdf947756https://www.arca.fiocruz.br/bitstream/icict/22875/2/suzana_cortereal_etal_IOC_2010.pdf373b4770e068a816d9b855b97eef4c63MD52TEXTsuzana_cortereal_etal_IOC_2010.pdf.txtsuzana_cortereal_etal_IOC_2010.pdf.txtExtracted 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| dc.title.pt_BR.fl_str_mv |
Kinetoplastid membrane protein-11 is present in promastigotes and amastigotes of Leishmania amazonensis and its surface expression increases during metacyclogenesis |
| title |
Kinetoplastid membrane protein-11 is present in promastigotes and amastigotes of Leishmania amazonensis and its surface expression increases during metacyclogenesis |
| spellingShingle |
Kinetoplastid membrane protein-11 is present in promastigotes and amastigotes of Leishmania amazonensis and its surface expression increases during metacyclogenesis Matos, Denise C. S. Leishmaniose metaciclogênese Expressão Proteínas de Membrana Leishmania amazonensis KMP-11 expression amastigote promastigote metacyclogenesis |
| title_short |
Kinetoplastid membrane protein-11 is present in promastigotes and amastigotes of Leishmania amazonensis and its surface expression increases during metacyclogenesis |
| title_full |
Kinetoplastid membrane protein-11 is present in promastigotes and amastigotes of Leishmania amazonensis and its surface expression increases during metacyclogenesis |
| title_fullStr |
Kinetoplastid membrane protein-11 is present in promastigotes and amastigotes of Leishmania amazonensis and its surface expression increases during metacyclogenesis |
| title_full_unstemmed |
Kinetoplastid membrane protein-11 is present in promastigotes and amastigotes of Leishmania amazonensis and its surface expression increases during metacyclogenesis |
| title_sort |
Kinetoplastid membrane protein-11 is present in promastigotes and amastigotes of Leishmania amazonensis and its surface expression increases during metacyclogenesis |
| author |
Matos, Denise C. S. |
| author_facet |
Matos, Denise C. S. Faccioli, Lanuza A. P. Finkelstein, Léa Cysne Luca, Paula Mello de Corte-Real, Suzana Armôa, Geraldo R. G. Lemes, Elezer Monte Blanco Ricardo, Debora Decote Mendonça, Sergio C. F. |
| author_role |
author |
| author2 |
Faccioli, Lanuza A. P. Finkelstein, Léa Cysne Luca, Paula Mello de Corte-Real, Suzana Armôa, Geraldo R. G. Lemes, Elezer Monte Blanco Ricardo, Debora Decote Mendonça, Sergio C. F. |
| author2_role |
author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Matos, Denise C. S. Faccioli, Lanuza A. P. Finkelstein, Léa Cysne Luca, Paula Mello de Corte-Real, Suzana Armôa, Geraldo R. G. Lemes, Elezer Monte Blanco Ricardo, Debora Decote Mendonça, Sergio C. F. |
| dc.subject.other.pt_BR.fl_str_mv |
Leishmaniose metaciclogênese Expressão Proteínas de Membrana |
| topic |
Leishmaniose metaciclogênese Expressão Proteínas de Membrana Leishmania amazonensis KMP-11 expression amastigote promastigote metacyclogenesis |
| dc.subject.en.pt_BR.fl_str_mv |
Leishmania amazonensis KMP-11 expression amastigote promastigote metacyclogenesis |
| description |
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunoparasitologia. Rio de Janeiro, RJ. Brasil. |
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2010 |
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2010 |
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2017-10-19T10:27:02Z |
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2017-10-19T10:27:02Z |
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info:eu-repo/semantics/article |
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article |
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MATOS, Denise C. S. et al. Kinetoplastid membrane protein-11 is present in promastigotes and amastigotes of Leishmania amazonensis and its surface expression increases during metacyclogenesis. Mem Inst Oswaldo Cruz, v.105, n.3, p.341-347, May 2010. |
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https://www.arca.fiocruz.br/handle/icict/22875 |
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0074-0276 |
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MATOS, Denise C. S. et al. Kinetoplastid membrane protein-11 is present in promastigotes and amastigotes of Leishmania amazonensis and its surface expression increases during metacyclogenesis. Mem Inst Oswaldo Cruz, v.105, n.3, p.341-347, May 2010. 0074-0276 |
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Fundação Oswaldo Cruz. Instituto Oswaldo Cruz |
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Fundação Oswaldo Cruz. Instituto Oswaldo Cruz |
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