Schistosoma mansoni rSm29 Antigen Induces a Regulatory Phenotype on Dendritic Cells and Lymphocytes From Patients With Cutaneous Leishmaniasis

Detalhes bibliográficos
Autor(a) principal: Lopes, Diego Mota
Data de Publicação: 2018
Outros Autores: Oliveira, Sérgio Costa, Page, Brady, Carvalho, Lucas Pedreira de, Carvalho Filho, Edgar Marcelino, Cardoso, Luciana Santos
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/32058
Resumo: Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq). This work was supported by the CNPq/MST/INCT-DT, grant number 465229/2014-0 and National Institutes of Health, grant number: AI30639-20.
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spelling Lopes, Diego MotaOliveira, Sérgio CostaPage, BradyCarvalho, Lucas Pedreira deCarvalho Filho, Edgar MarcelinoCardoso, Luciana Santos2019-03-13T12:24:24Z2019-03-13T12:24:24Z2018LOPES, D. M. et al. Schistosoma mansoni rSm29 Antigen Induces a Regulatory Phenotype on Dendritic Cells and Lymphocytes From Patients With Cutaneous Leishmaniasis. Frontiers in Immunology, v. 9, p. 3122, 2019.1664-3224https://www.arca.fiocruz.br/handle/icict/3205810.3389/fimmu.2018.03122Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq). This work was supported by the CNPq/MST/INCT-DT, grant number 465229/2014-0 and National Institutes of Health, grant number: AI30639-20.Universidade Federal da Bahia. Hospitalar Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, Brasil / Instituto Nacional de Ciência e Tecnologia em Doenças Tropicais. Brasilia, DF, Brasil.Universidade Federal de Minas Gerais. Instituto de ciências Biológicas. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brasil / Instituto Nacional de Ciências e Tecnologia em Doenças Tropicais. Brasilia, DF, Brasil.Massachusetts General Hospital. Boston, MA, United States.Universidade Federal da Bahia. Hospitalar Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, Brasil / Instituto Nacional de Ciência e Tecnologia em Doenças Tropicais. Brasilia, DF, Brasil / Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.Universidade Federal da Bahia. Hospitalar Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, Brasil / Instituto Nacional de Ciência e Tecnologia em Doenças Tropicais. Brasilia, DF, Brasil / Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.Universidade Federal da Bahia. Hospitalar Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, Brasil / Instituto Nacional de Ciência e Tecnologia em Doenças Tropicais. Brasilia, DF, Brasil / Universidade Federal da Bahia. Faculdade de Farmácia. Departamento de Análises Clínicas e Toxicológicas. Salvador, BA, Brasil.The immune response induced by Schistosma mansoni antigens is able to prevent immune-mediated diseases. Conversely, the inflammatory response in cutaneous leishmaniasis (CL), although responsible for controlling the infection, is also associated with the pathogenesis of disease. The aim of this study was to evaluate the potential of the S. mansoni Sm29 antigen to change certain aspects of the profiles of monocyte derived dendritic cells (MoDCs) and lymphocytes from subjects with CL in vitro. Expression of surface molecules and intracellular cytokines in the MoDCs and lymphocytes as well as the proliferation of Leishmania braziliensis were evaluated by flow cytometry. Levels of cytokines were evaluated in culture supernatants by ELISA. It was observed that stimulation by rSm29 increased the frequency of expression of CD83, CD80, CD86, and IL-10R in MoDCs compared to non-stimulated cultures. Additionally rSm29 decreased the frequency CD4+ and CD8+ T cells expressing CD28 and increased the frequency of CD4+CD25hi and CD4+CTLA-4+ T lymphocytes. Addition of rSm29 to cultures increased IL-10 levels and decreased levels of IL-12p40 and IFN-γ, while not altering TNF levels compared to non-stimulated cultures. This study showed that rSm29 induced a regulatory profile in MoDCs and lymphocytes and thereby regulated the exaggerated inflammation observed in CL. Considering that there are few therapeutic options for leishmaniasis, the use of rSm29 may be an alternative to current treatment and may be an important strategy to reduce the healing time of lesions in patients with CL.engFrontiers MediaSchistosoma mansoniLeishmaniose cutâneaAntígeno Sm29Células dendríticasLinfócitosSchistosoma mansoniCutaneous leishmaniasisSm29 antigenDendritic cellsLymphocytesSchistosoma mansoni rSm29 Antigen Induces a Regulatory Phenotype on Dendritic Cells and Lymphocytes From Patients With Cutaneous Leishmaniasisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/32058/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALLopes D.M Schistosoma mansoni rSm29...2019.pdfLopes D.M Schistosoma mansoni rSm29...2019.pdfapplication/pdf2278024https://www.arca.fiocruz.br/bitstream/icict/32058/2/Lopes%20D.M%20Schistosoma%20mansoni%20rSm29...2019.pdf7bdb61cb365b8968429f4a5ee0f9a85aMD52TEXTLopes D.M Schistosoma mansoni rSm29...2019.pdf.txtLopes D.M Schistosoma mansoni rSm29...2019.pdf.txtExtracted texttext/plain60451https://www.arca.fiocruz.br/bitstream/icict/32058/3/Lopes%20D.M%20Schistosoma%20mansoni%20rSm29...2019.pdf.txte23ac969fd331cdd57e12f36ff06085eMD53icict/320582023-03-15 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dc.title.pt_BR.fl_str_mv Schistosoma mansoni rSm29 Antigen Induces a Regulatory Phenotype on Dendritic Cells and Lymphocytes From Patients With Cutaneous Leishmaniasis
title Schistosoma mansoni rSm29 Antigen Induces a Regulatory Phenotype on Dendritic Cells and Lymphocytes From Patients With Cutaneous Leishmaniasis
spellingShingle Schistosoma mansoni rSm29 Antigen Induces a Regulatory Phenotype on Dendritic Cells and Lymphocytes From Patients With Cutaneous Leishmaniasis
Lopes, Diego Mota
Schistosoma mansoni
Leishmaniose cutânea
Antígeno Sm29
Células dendríticas
Linfócitos
Schistosoma mansoni
Cutaneous leishmaniasis
Sm29 antigen
Dendritic cells
Lymphocytes
title_short Schistosoma mansoni rSm29 Antigen Induces a Regulatory Phenotype on Dendritic Cells and Lymphocytes From Patients With Cutaneous Leishmaniasis
title_full Schistosoma mansoni rSm29 Antigen Induces a Regulatory Phenotype on Dendritic Cells and Lymphocytes From Patients With Cutaneous Leishmaniasis
title_fullStr Schistosoma mansoni rSm29 Antigen Induces a Regulatory Phenotype on Dendritic Cells and Lymphocytes From Patients With Cutaneous Leishmaniasis
title_full_unstemmed Schistosoma mansoni rSm29 Antigen Induces a Regulatory Phenotype on Dendritic Cells and Lymphocytes From Patients With Cutaneous Leishmaniasis
title_sort Schistosoma mansoni rSm29 Antigen Induces a Regulatory Phenotype on Dendritic Cells and Lymphocytes From Patients With Cutaneous Leishmaniasis
author Lopes, Diego Mota
author_facet Lopes, Diego Mota
Oliveira, Sérgio Costa
Page, Brady
Carvalho, Lucas Pedreira de
Carvalho Filho, Edgar Marcelino
Cardoso, Luciana Santos
author_role author
author2 Oliveira, Sérgio Costa
Page, Brady
Carvalho, Lucas Pedreira de
Carvalho Filho, Edgar Marcelino
Cardoso, Luciana Santos
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Lopes, Diego Mota
Oliveira, Sérgio Costa
Page, Brady
Carvalho, Lucas Pedreira de
Carvalho Filho, Edgar Marcelino
Cardoso, Luciana Santos
dc.subject.other.pt_BR.fl_str_mv Schistosoma mansoni
Leishmaniose cutânea
Antígeno Sm29
Células dendríticas
Linfócitos
topic Schistosoma mansoni
Leishmaniose cutânea
Antígeno Sm29
Células dendríticas
Linfócitos
Schistosoma mansoni
Cutaneous leishmaniasis
Sm29 antigen
Dendritic cells
Lymphocytes
dc.subject.en.pt_BR.fl_str_mv Schistosoma mansoni
Cutaneous leishmaniasis
Sm29 antigen
Dendritic cells
Lymphocytes
description Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq). This work was supported by the CNPq/MST/INCT-DT, grant number 465229/2014-0 and National Institutes of Health, grant number: AI30639-20.
publishDate 2018
dc.date.issued.fl_str_mv 2018
dc.date.accessioned.fl_str_mv 2019-03-13T12:24:24Z
dc.date.available.fl_str_mv 2019-03-13T12:24:24Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv LOPES, D. M. et al. Schistosoma mansoni rSm29 Antigen Induces a Regulatory Phenotype on Dendritic Cells and Lymphocytes From Patients With Cutaneous Leishmaniasis. Frontiers in Immunology, v. 9, p. 3122, 2019.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/32058
dc.identifier.issn.pt_BR.fl_str_mv 1664-3224
dc.identifier.doi.none.fl_str_mv 10.3389/fimmu.2018.03122
identifier_str_mv LOPES, D. M. et al. Schistosoma mansoni rSm29 Antigen Induces a Regulatory Phenotype on Dendritic Cells and Lymphocytes From Patients With Cutaneous Leishmaniasis. Frontiers in Immunology, v. 9, p. 3122, 2019.
1664-3224
10.3389/fimmu.2018.03122
url https://www.arca.fiocruz.br/handle/icict/32058
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
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collection Repositório Institucional da FIOCRUZ (ARCA)
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