Escherichia coli Braun Lipoprotein (BLP) exhibits endotoxemia - like pathology in Swiss albino mice
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2016 |
| Outros Autores: | , , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/17908 |
Resumo: | University of Mysore. Department of Studies in Biochemistry. Karnataka, India. |
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Lakshmikanth, Chikkamenahalli LakshminarayanaPetsel Jacob, ShancyKudva, Avinash KundadkaLatchoumycandane, CalivarathanYashaswini, Puttaraju Srikanta MurthySumanth, Mosale SeetharamAlbuquerque, Cassiano F. Gonçalves deSilva, Adriana R.Singh, Sridevi AnnapurnaFaria Neto, Hugo C. CastroPrabhu, Sandeep KumbleMcIntyre, Thomas M.Marathe, Gopal Kedihithlu2017-03-01T14:45:23Z2017-03-01T14:45:23Z2016Lakshmikanth, Chikkamenahalli Lakshminarayan; et al. Escherichia coli Braun Lipoprotein (BLP) exhibits endotoxemia – like pathology in Swiss albino mice. Sci Rep., v.6:34666, 13p, Oct. 2016.2045-2322https://www.arca.fiocruz.br/handle/icict/1790810.1038/srep34666engNature Publishing GroupEscherichia coliendotoxemiaLipoproteínasCamundongosEscherichia coli Braun Lipoprotein (BLP)Swiss albino miceendotoxemiaEscherichia coli Braun Lipoprotein (BLP) exhibits endotoxemia - like pathology in Swiss albino miceinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversity of Mysore. Department of Studies in Biochemistry. Karnataka, India.University of Mysore. Department of Studies in Biochemistry. Karnataka, India.The Pennsylvania State University. Center for Molecular Immunology and Infectious Disease and Center for Molecular Toxicology and Carcinogenesis. Department of Veterinary and Biomedical Sciences. University Park, PA, USA.Cleveland Clinic Lerner Research Institute. Department of Cellular and Molecular Medicine. Cleveland, Ohio, USA.Central Food Technological Research Institute/CSIR. Department of Protein Chemistry & Technology. Karnataka, India.University of Mysore. Department of Studies in Biochemistry. Karnataka, India.Fundação Oswado Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil.Fundação Oswado Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil.University of Mysore. Department of Studies in Biochemistry. Karnataka, India.Fundação Oswado Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil.The Pennsylvania State University. Center for Molecular Immunology and Infectious Disease and Center for Molecular Toxicology and Carcinogenesis. Department of Veterinary and Biomedical Sciences. University Park, PA, USA.Cleveland Clinic Lerner Research Institute. Department of Cellular and Molecular Medicine. Cleveland, Ohio, USA.University of Mysore. Department of Studies in Biochemistry. Karnataka, India.The endotoxin lipopolysaccharide (LPS) promotes sepsis, but bacterial peptides also promote inflammation leading to sepsis. We found, intraperitoneal administration of live or heat inactivated E. coli JE5505 lacking the abundant outer membrane protein, Braun lipoprotein (BLP), was less toxic than E. coli DH5α possessing BLP in Swiss albino mice. Injection of BLP free of LPS purified from E. coli DH5α induced massive infiltration of leukocytes in lungs and liver. BLP activated human polymorphonuclear cells (PMNs) ex vivo to adhere to denatured collagen in serum and polymyxin B independent fashion, a property distinct from LPS. Both LPS and BLP stimulated the synthesis of platelet activating factor (PAF), a potent lipid mediator, in human PMNs. In mouse macrophage cell line, RAW264.7, while both BLP and LPS similarly upregulated TNF-α and IL-1β mRNA; BLP was more potent in inducing cyclooxygenase-2 (COX-2) mRNA and protein expression. Peritoneal macrophages from TLR2(-/-) mice significantly reduced the production of TNF-α in response to BLP in contrast to macrophages from wild type mice. We conclude, BLP acting through TLR2, is a potent inducer of inflammation with a response profile both common and distinct from LPS. Hence, BLP mediated pathway may also be considered as an effective target against sepsis.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/17908/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALhugo3_farianeto_etal_IOC_2016.pdfhugo3_farianeto_etal_IOC_2016.pdfapplication/pdf1958922https://www.arca.fiocruz.br/bitstream/icict/17908/2/hugo3_farianeto_etal_IOC_2016.pdf8f0de3211baf4ed452f0a76e4b91eb3cMD52srep34666-s1.docsrep34666-s1.docMaterial Suplementarapplication/msword1506816https://www.arca.fiocruz.br/bitstream/icict/17908/3/srep34666-s1.doc6ef1d512c6cd3cc4b974585182122cc7MD53TEXThugo3_farianeto_etal_IOC_2016.pdf.txthugo3_farianeto_etal_IOC_2016.pdf.txtExtracted 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| dc.title.pt_BR.fl_str_mv |
Escherichia coli Braun Lipoprotein (BLP) exhibits endotoxemia - like pathology in Swiss albino mice |
| title |
Escherichia coli Braun Lipoprotein (BLP) exhibits endotoxemia - like pathology in Swiss albino mice |
| spellingShingle |
Escherichia coli Braun Lipoprotein (BLP) exhibits endotoxemia - like pathology in Swiss albino mice Lakshmikanth, Chikkamenahalli Lakshminarayana Escherichia coli endotoxemia Lipoproteínas Camundongos Escherichia coli Braun Lipoprotein (BLP) Swiss albino mice endotoxemia |
| title_short |
Escherichia coli Braun Lipoprotein (BLP) exhibits endotoxemia - like pathology in Swiss albino mice |
| title_full |
Escherichia coli Braun Lipoprotein (BLP) exhibits endotoxemia - like pathology in Swiss albino mice |
| title_fullStr |
Escherichia coli Braun Lipoprotein (BLP) exhibits endotoxemia - like pathology in Swiss albino mice |
| title_full_unstemmed |
Escherichia coli Braun Lipoprotein (BLP) exhibits endotoxemia - like pathology in Swiss albino mice |
| title_sort |
Escherichia coli Braun Lipoprotein (BLP) exhibits endotoxemia - like pathology in Swiss albino mice |
| author |
Lakshmikanth, Chikkamenahalli Lakshminarayana |
| author_facet |
Lakshmikanth, Chikkamenahalli Lakshminarayana Petsel Jacob, Shancy Kudva, Avinash Kundadka Latchoumycandane, Calivarathan Yashaswini, Puttaraju Srikanta Murthy Sumanth, Mosale Seetharam Albuquerque, Cassiano F. Gonçalves de Silva, Adriana R. Singh, Sridevi Annapurna Faria Neto, Hugo C. Castro Prabhu, Sandeep Kumble McIntyre, Thomas M. Marathe, Gopal Kedihithlu |
| author_role |
author |
| author2 |
Petsel Jacob, Shancy Kudva, Avinash Kundadka Latchoumycandane, Calivarathan Yashaswini, Puttaraju Srikanta Murthy Sumanth, Mosale Seetharam Albuquerque, Cassiano F. Gonçalves de Silva, Adriana R. Singh, Sridevi Annapurna Faria Neto, Hugo C. Castro Prabhu, Sandeep Kumble McIntyre, Thomas M. Marathe, Gopal Kedihithlu |
| author2_role |
author author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Lakshmikanth, Chikkamenahalli Lakshminarayana Petsel Jacob, Shancy Kudva, Avinash Kundadka Latchoumycandane, Calivarathan Yashaswini, Puttaraju Srikanta Murthy Sumanth, Mosale Seetharam Albuquerque, Cassiano F. Gonçalves de Silva, Adriana R. Singh, Sridevi Annapurna Faria Neto, Hugo C. Castro Prabhu, Sandeep Kumble McIntyre, Thomas M. Marathe, Gopal Kedihithlu |
| dc.subject.other.pt_BR.fl_str_mv |
Escherichia coli endotoxemia Lipoproteínas Camundongos |
| topic |
Escherichia coli endotoxemia Lipoproteínas Camundongos Escherichia coli Braun Lipoprotein (BLP) Swiss albino mice endotoxemia |
| dc.subject.en.pt_BR.fl_str_mv |
Escherichia coli Braun Lipoprotein (BLP) Swiss albino mice endotoxemia |
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University of Mysore. Department of Studies in Biochemistry. Karnataka, India. |
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2016 |
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2016 |
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2017-03-01T14:45:23Z |
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2017-03-01T14:45:23Z |
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info:eu-repo/semantics/article |
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Lakshmikanth, Chikkamenahalli Lakshminarayan; et al. Escherichia coli Braun Lipoprotein (BLP) exhibits endotoxemia – like pathology in Swiss albino mice. Sci Rep., v.6:34666, 13p, Oct. 2016. |
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https://www.arca.fiocruz.br/handle/icict/17908 |
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2045-2322 |
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10.1038/srep34666 |
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Lakshmikanth, Chikkamenahalli Lakshminarayan; et al. Escherichia coli Braun Lipoprotein (BLP) exhibits endotoxemia – like pathology in Swiss albino mice. Sci Rep., v.6:34666, 13p, Oct. 2016. 2045-2322 10.1038/srep34666 |
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