Evaluation of na Enzyme Linked Immunosorbent Assay (ELISA) using purified, deglycosylated histoplamin for different clinical manifestations of histoplamosis

Detalhes bibliográficos
Autor(a) principal: Guimarães, Allan Jefferson
Data de Publicação: 2010
Outros Autores: Pizzini, Claudia Vera, Almeida, Marcos de Abreu, Peralta, José Mauro, Nosanchuk, Joshua Daniel, Zancope-Oliveira, Rosely Maria
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/37747
Resumo: Yeshiva University. Albert Einstein College of Medicine. Department of Medicine & Microbiology and Immunology. Division of Infectious Diseases. New York, NY, USA.
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spelling Guimarães, Allan JeffersonPizzini, Claudia VeraAlmeida, Marcos de AbreuPeralta, José MauroNosanchuk, Joshua DanielZancope-Oliveira, Rosely Maria2019-12-10T13:52:40Z2019-12-10T13:52:40Z2010GUIMARÃES, Allan Jefferson et al. Evaluation of an enzyme-linked immunosorbent assay using purified, deglycosylated histoplasmin for different clinical manifestations of histoplasmosis. Microbiology Research, v. 1, n. 1, p. 1-21, 2010.2036-7481https://www.arca.fiocruz.br/handle/icict/3774710.4081/mr.2010.e22036-7473engPAGEpressEvaluation of na Enzyme Linked Immunosorbent Assay (ELISA) using purified, deglycosylated histoplamin for different clinical manifestations of histoplamosisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleYeshiva University. Albert Einstein College of Medicine. Department of Medicine & Microbiology and Immunology. Division of Infectious Diseases. New York, NY, USA.Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Laboratório de Micologia. Setor de Imunodiagnóstico. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Laboratório de Micologia. Setor de Imunodiagnóstico. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Prof. Paulo de Góes. Rio de Janeiro, RJ, Brasil.Yeshiva University. Albert Einstein College of Medicine. Department of Medicine & Microbiology and Immunology. Division of Infectious Diseases. New York, NY, USA.Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Laboratório de Micologia. Setor de Imunodiagnóstico. Rio de Janeiro, RJ, Brasil.Diagnosis of invasive fungal diseases remains problematic, especially in undeveloped countries. We have developed an enzyme-linked immunosorbent assay (ELISA) for the detection of antibodies to Histoplasma capsulatum using metaperiodate treated purified histoplasmin (ptHMIN). Our ELISA was validated comparing sera from patients with histoplasmosis, related mycoses, and healthy individuals. The overall test specificity was 96%, with sensitivities of 100% (8/8) in acute disease, 90% (9/10) in chronic disease, 89% (8/9) in disseminated infection in individuals without HIV infection, 86% (12/14) in disseminated disease in the setting of HIV infection and 100% (3/3) in mediastinal histoplasmosis. These parameters are superior to the use of untreated histoplasmin in diagnostic ELISAs. The high specificities, sensitivities, and simplicity of our ELISA support further development of a deglycosylated HMIN ELISA for clinical use and for monitoring the humoral immune response during therapy in patients with chronic and disseminated histoplasmosis.ELISAAntibody detectionHistoplasmosisSerological diagnosisinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txttext/plain1748https://www.arca.fiocruz.br/bitstream/icict/37747/1/license.txt8a4605be74aa9ea9d79846c1fba20a33MD51ORIGINALva_Guimarães_Allan_etal_INI_2010.pdfva_Guimarães_Allan_etal_INI_2010.pdfapplication/pdf499129https://www.arca.fiocruz.br/bitstream/icict/37747/2/va_Guimar%c3%a3es_Allan_etal_INI_2010.pdf9e8b3bb1bbcd366e32e8691d2c58f2a1MD52TEXTva_Guimarães_Allan_etal_INI_2010.pdf.txtva_Guimarães_Allan_etal_INI_2010.pdf.txtExtracted texttext/plain50170https://www.arca.fiocruz.br/bitstream/icict/37747/3/va_Guimar%c3%a3es_Allan_etal_INI_2010.pdf.txtf703cf3efee947eff47b1128df4f422aMD53icict/377472019-12-11 02:00:45.868oai:www.arca.fiocruz.br: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Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352019-12-11T05:00:45Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Evaluation of na Enzyme Linked Immunosorbent Assay (ELISA) using purified, deglycosylated histoplamin for different clinical manifestations of histoplamosis
title Evaluation of na Enzyme Linked Immunosorbent Assay (ELISA) using purified, deglycosylated histoplamin for different clinical manifestations of histoplamosis
spellingShingle Evaluation of na Enzyme Linked Immunosorbent Assay (ELISA) using purified, deglycosylated histoplamin for different clinical manifestations of histoplamosis
Guimarães, Allan Jefferson
ELISA
Antibody detection
Histoplasmosis
Serological diagnosis
title_short Evaluation of na Enzyme Linked Immunosorbent Assay (ELISA) using purified, deglycosylated histoplamin for different clinical manifestations of histoplamosis
title_full Evaluation of na Enzyme Linked Immunosorbent Assay (ELISA) using purified, deglycosylated histoplamin for different clinical manifestations of histoplamosis
title_fullStr Evaluation of na Enzyme Linked Immunosorbent Assay (ELISA) using purified, deglycosylated histoplamin for different clinical manifestations of histoplamosis
title_full_unstemmed Evaluation of na Enzyme Linked Immunosorbent Assay (ELISA) using purified, deglycosylated histoplamin for different clinical manifestations of histoplamosis
title_sort Evaluation of na Enzyme Linked Immunosorbent Assay (ELISA) using purified, deglycosylated histoplamin for different clinical manifestations of histoplamosis
author Guimarães, Allan Jefferson
author_facet Guimarães, Allan Jefferson
Pizzini, Claudia Vera
Almeida, Marcos de Abreu
Peralta, José Mauro
Nosanchuk, Joshua Daniel
Zancope-Oliveira, Rosely Maria
author_role author
author2 Pizzini, Claudia Vera
Almeida, Marcos de Abreu
Peralta, José Mauro
Nosanchuk, Joshua Daniel
Zancope-Oliveira, Rosely Maria
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Guimarães, Allan Jefferson
Pizzini, Claudia Vera
Almeida, Marcos de Abreu
Peralta, José Mauro
Nosanchuk, Joshua Daniel
Zancope-Oliveira, Rosely Maria
dc.subject.en.pt_BR.fl_str_mv ELISA
Antibody detection
Histoplasmosis
Serological diagnosis
topic ELISA
Antibody detection
Histoplasmosis
Serological diagnosis
description Yeshiva University. Albert Einstein College of Medicine. Department of Medicine & Microbiology and Immunology. Division of Infectious Diseases. New York, NY, USA.
publishDate 2010
dc.date.issued.fl_str_mv 2010
dc.date.accessioned.fl_str_mv 2019-12-10T13:52:40Z
dc.date.available.fl_str_mv 2019-12-10T13:52:40Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv GUIMARÃES, Allan Jefferson et al. Evaluation of an enzyme-linked immunosorbent assay using purified, deglycosylated histoplasmin for different clinical manifestations of histoplasmosis. Microbiology Research, v. 1, n. 1, p. 1-21, 2010.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/37747
dc.identifier.issn.pt_BR.fl_str_mv 2036-7481
dc.identifier.doi.none.fl_str_mv 10.4081/mr.2010.e2
dc.identifier.eissn.none.fl_str_mv 2036-7473
identifier_str_mv GUIMARÃES, Allan Jefferson et al. Evaluation of an enzyme-linked immunosorbent assay using purified, deglycosylated histoplasmin for different clinical manifestations of histoplasmosis. Microbiology Research, v. 1, n. 1, p. 1-21, 2010.
2036-7481
10.4081/mr.2010.e2
2036-7473
url https://www.arca.fiocruz.br/handle/icict/37747
dc.language.iso.fl_str_mv eng
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dc.publisher.none.fl_str_mv PAGEpress
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