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spelling Rossi, Átila DuqueAraújo, João Locke Ferreira deAlmeida, Tailah Bernardo deRibeiro-Alves, MarceloVelozo, Camila de AlmeidaAlmeida, Jéssica Maciel deLeitão, Isabela de CarvalhoFerreira, Sâmila NatianeOliveira, Jéssica da SilvaAlves, Hugo JoséScheid, Helena ToledoFaffe, Débora SouzaGalliez, Rafael MelloÁvila, Renata Eliane deResende, Gustavo GomesTeixeira, Mauro MartinsFerreira Júnior, Orlando da CostaCastiñeiras, Terezinha Marta P. P.Souza, Renan PedraTanuri, AmilcarAguiar, Renato Santana deBarroso, Shana Priscila CoutinhoCardoso, Cynthia ChesterCOVID-19 UFRJ Workgroup2021-06-18T12:20:47Z2021-06-18T12:20:47Z2021ROSSI, Átila Duque et al. Association between ACE2 and TMPRSS2 nasopharyngeal expression and COVID-19 respiratory distress. Scientific reports v. 11, n. 1, p. 1-9, 20212045-2322https://www.arca.fiocruz.br/handle/icict/4775810.1038/s41598-021-88944-8engNatureAssociation between ACE2 and TMPRSS2 nasopharyngeal expression and COVID-19 respiratory distressinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal do Rio de Janeiro. Instituto de Biologia. Departamento de Genética. Laboratório de Virologia Molecular. Rio de Janeiro, RJ, Brasil.Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Genética, Ecologia e Evolução. Laboratório de Biologia Integrativa. Belo Horizonte, MG, Brasil.Marinha do Brasil. Instituto de Estudos do Mar Almirante Paulo Moreira. Arraial do Cabo, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Pesquisa Clínica em DST/AIDS. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Biologia. Departamento de Genética. Laboratório de Virologia Molecular. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Biologia. Departamento de Genética. Laboratório de Virologia Molecular. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Biofísica. Rio de Janeiro, RJ, Brasil.Marinha do Brasil. Hospital Naval Marcílio Dias. Instituto de Pesquisas Biomédicas. Rio de Janeiro, RJ, Brasil.Marinha do Brasil. Hospital Naval Marcílio Dias. Instituto de Pesquisas Biomédicas. Rio de Janeiro, RJ, Brasil.Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Genética, Ecologia e Evolução. Laboratório de Biologia Integrativa. Belo Horizonte, MG, Brasil.Universidade Federal do Rio de Janeiro. Faculdade de Medicina. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Biofísica. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Faculdade de Medicina. Rio de Janeiro, RJ, Brasil.Hospital Eduardo de Menezes. Belo Horizonte, MG, Brasil.Universidade Federal de Minas Gerais. Hospital das Clínicas. Belo Horizonte, MG, Brasil.Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Biologia. Departamento de Genética. Laboratório de Virologia Molecular. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Faculdade de Medicina. Rio de Janeiro, RJ, Brasil.Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Genética, Ecologia e Evolução. Laboratório de Biologia Integrativa. Belo Horizonte, MG, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Biologia. Departamento de Genética. Laboratório de Virologia Molecular. Rio de Janeiro, RJ, Brasil.Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Genética, Ecologia e Evolução. Laboratório de Biologia Integrativa. Belo Horizonte, MG, Brasil.Marinha do Brasil. Hospital Naval Marcílio Dias. Instituto de Pesquisas Biomédicas. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Biologia. Departamento de Genética. Laboratório de Virologia Molecular. Rio de Janeiro, RJ, Brasil.ACE2 and TMPRSS2 are key players on SARS-CoV-2 entry into host cells. However, it is still unclear whether expression levels of these factors could reflect disease severity. Here, a case-control study was conducted with 213 SARS-CoV-2 positive individuals where cases were defined as COVID-19 patients with respiratory distress requiring oxygen support (N = 38) and controls were those with mild to moderate symptoms of the disease who did not need oxygen therapy along the entire clinical course (N = 175). ACE2 and TMPRSS2 mRNA levels were evaluated in nasopharyngeal swab samples by RT-qPCR and logistic regression analyzes were applied to estimate associations with respiratory outcomes. ACE2 and TMPRSS2 levels positively correlated with age, which was also strongly associated with respiratory distress. Increased nasopharyngeal ACE2 levels showed a protective effect against this outcome (adjOR = 0.30; 95% CI 0.09-0.91), while TMPRSS2/ACE2 ratio was associated with risk (adjOR = 4.28; 95% CI 1.36-13.48). On stepwise regression, TMPRSS2/ACE2 ratio outperformed ACE2 to model COVID-19 severity. When nasopharyngeal swabs were compared to bronchoalveolar lavages in an independent cohort of COVID-19 patients under mechanical ventilation, similar expression levels of these genes were observed. These data suggest nasopharyngeal TMPRSS2/ACE2 as a promising candidate for further prediction models on COVID-19.SARS-CoV-2VirologyViral infectionGene expressionInfectious diseasesRespiratory tract diseasesEpidemiologyTranscriptioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83099https://www.arca.fiocruz.br/bitstream/icict/47758/1/license.txt586c046dcfeef936e32f0323bb9a47c0MD51ORIGINALAssociation_Marcelo_Alves_etal_INI_2021_COVID-19.pdfAssociation_Marcelo_Alves_etal_INI_2021_COVID-19.pdfapplication/pdf1242184https://www.arca.fiocruz.br/bitstream/icict/47758/2/Association_Marcelo_Alves_etal_INI_2021_COVID-19.pdfa56835f0434c4b3f633c7fbafc203459MD52TEXTAssociation_Marcelo_Alves_etal_INI_2021_COVID-19.pdf.txtAssociation_Marcelo_Alves_etal_INI_2021_COVID-19.pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv Association between ACE2 and TMPRSS2 nasopharyngeal expression and COVID-19 respiratory distress
title Association between ACE2 and TMPRSS2 nasopharyngeal expression and COVID-19 respiratory distress
spellingShingle Association between ACE2 and TMPRSS2 nasopharyngeal expression and COVID-19 respiratory distress
Rossi, Átila Duque
SARS-CoV-2
Virology
Viral infection
Gene expression
Infectious diseases
Respiratory tract diseases
Epidemiology
Transcription
title_short Association between ACE2 and TMPRSS2 nasopharyngeal expression and COVID-19 respiratory distress
title_full Association between ACE2 and TMPRSS2 nasopharyngeal expression and COVID-19 respiratory distress
title_fullStr Association between ACE2 and TMPRSS2 nasopharyngeal expression and COVID-19 respiratory distress
title_full_unstemmed Association between ACE2 and TMPRSS2 nasopharyngeal expression and COVID-19 respiratory distress
title_sort Association between ACE2 and TMPRSS2 nasopharyngeal expression and COVID-19 respiratory distress
author Rossi, Átila Duque
author_facet Rossi, Átila Duque
Araújo, João Locke Ferreira de
Almeida, Tailah Bernardo de
Ribeiro-Alves, Marcelo
Velozo, Camila de Almeida
Almeida, Jéssica Maciel de
Leitão, Isabela de Carvalho
Ferreira, Sâmila Natiane
Oliveira, Jéssica da Silva
Alves, Hugo José
Scheid, Helena Toledo
Faffe, Débora Souza
Galliez, Rafael Mello
Ávila, Renata Eliane de
Resende, Gustavo Gomes
Teixeira, Mauro Martins
Ferreira Júnior, Orlando da Costa
Castiñeiras, Terezinha Marta P. P.
Souza, Renan Pedra
Tanuri, Amilcar
Aguiar, Renato Santana de
Barroso, Shana Priscila Coutinho
Cardoso, Cynthia Chester
COVID-19 UFRJ Workgroup
author_role author
author2 Araújo, João Locke Ferreira de
Almeida, Tailah Bernardo de
Ribeiro-Alves, Marcelo
Velozo, Camila de Almeida
Almeida, Jéssica Maciel de
Leitão, Isabela de Carvalho
Ferreira, Sâmila Natiane
Oliveira, Jéssica da Silva
Alves, Hugo José
Scheid, Helena Toledo
Faffe, Débora Souza
Galliez, Rafael Mello
Ávila, Renata Eliane de
Resende, Gustavo Gomes
Teixeira, Mauro Martins
Ferreira Júnior, Orlando da Costa
Castiñeiras, Terezinha Marta P. P.
Souza, Renan Pedra
Tanuri, Amilcar
Aguiar, Renato Santana de
Barroso, Shana Priscila Coutinho
Cardoso, Cynthia Chester
COVID-19 UFRJ Workgroup
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Rossi, Átila Duque
Araújo, João Locke Ferreira de
Almeida, Tailah Bernardo de
Ribeiro-Alves, Marcelo
Velozo, Camila de Almeida
Almeida, Jéssica Maciel de
Leitão, Isabela de Carvalho
Ferreira, Sâmila Natiane
Oliveira, Jéssica da Silva
Alves, Hugo José
Scheid, Helena Toledo
Faffe, Débora Souza
Galliez, Rafael Mello
Ávila, Renata Eliane de
Resende, Gustavo Gomes
Teixeira, Mauro Martins
Ferreira Júnior, Orlando da Costa
Castiñeiras, Terezinha Marta P. P.
Souza, Renan Pedra
Tanuri, Amilcar
Aguiar, Renato Santana de
Barroso, Shana Priscila Coutinho
Cardoso, Cynthia Chester
COVID-19 UFRJ Workgroup
dc.subject.en.pt_BR.fl_str_mv SARS-CoV-2
Virology
Viral infection
Gene expression
Infectious diseases
Respiratory tract diseases
Epidemiology
Transcription
topic SARS-CoV-2
Virology
Viral infection
Gene expression
Infectious diseases
Respiratory tract diseases
Epidemiology
Transcription
description Universidade Federal do Rio de Janeiro. Instituto de Biologia. Departamento de Genética. Laboratório de Virologia Molecular. Rio de Janeiro, RJ, Brasil.
publishDate 2021
dc.date.accessioned.fl_str_mv 2021-06-18T12:20:47Z
dc.date.available.fl_str_mv 2021-06-18T12:20:47Z
dc.date.issued.fl_str_mv 2021
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv ROSSI, Átila Duque et al. Association between ACE2 and TMPRSS2 nasopharyngeal expression and COVID-19 respiratory distress. Scientific reports v. 11, n. 1, p. 1-9, 2021
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/47758
dc.identifier.issn.pt_BR.fl_str_mv 2045-2322
dc.identifier.doi.none.fl_str_mv 10.1038/s41598-021-88944-8
identifier_str_mv ROSSI, Átila Duque et al. Association between ACE2 and TMPRSS2 nasopharyngeal expression and COVID-19 respiratory distress. Scientific reports v. 11, n. 1, p. 1-9, 2021
2045-2322
10.1038/s41598-021-88944-8
url https://www.arca.fiocruz.br/handle/icict/47758
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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collection Repositório Institucional da FIOCRUZ (ARCA)
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