Biochemical and functional characterization of parawixia bistriata spider venom with potential proteolytic and larvicidal activities
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
Texto Completo: | https://www.arca.fiocruz.br/handle/icict/14300 http://dx.doi.org/10.1155/2014/950538 |
Resumo: | Fundação Oswaldo Cruz. Centro de Estudos de Biomoléculas Aplicadas a Saúde. Departamento de Medicina. Universidade Federal de Rondônia. Porto Velho, RO, Brazil. |
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Gimenez, Gizeli S.Coutinho-Neto, AntonioKayano, Anderson M.Silva, Rodrigo SimõesTrindade, FrancesSilva, Alexandre de Almeida eMarcussi, SilvanaSilva, Saulo L. daFernandes, Carla F. C.Zuliani, Juliana P.Calderon, Leonardo A.Soares, Andreimar M.Stábeli, Rodrigo G.2016-05-16T21:12:26Z2016-05-16T21:12:26Z2014SOARES, A.M., et al. Biochemical and Functional Characterization of Parawixia bistriata Spider Venom with Potential Proteolytic and Larvicidal Activities. BioMed Research International, v. 2014, p. 1-13, 2014.https://www.arca.fiocruz.br/handle/icict/14300http://dx.doi.org/10.1155/2014/950538engBioMed Research InternationalBiochemical and functional characterization of parawixia bistriata spider venom with potential proteolytic and larvicidal activitiesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Centro de Estudos de Biomoléculas Aplicadas a Saúde. Departamento de Medicina. Universidade Federal de Rondônia. Porto Velho, RO, Brazil.Fundação Oswaldo Cruz. Centro de Estudos de Biomoléculas Aplicadas a Saúde. Departamento de Medicina. Universidade Federal de Rondônia. Porto Velho, RO, Brazil.Fundação Oswaldo Cruz. Centro de Estudos de Biomoléculas Aplicadas a Saúde. Departamento de Medicina. Universidade Federal de Rondônia. Porto Velho, RO, Brazil.Fundação Oswaldo Cruz. Centro de Estudos de Biomoléculas Aplicadas a Saúde. Departamento de Medicina. Universidade Federal de Rondônia. Porto Velho, RO, Brazil.Fundação Oswaldo Cruz. Departamento de Biologia. Laboratório de Entomologia Médica. Laboratório de Bioecologia de Insetos. Porto Velho, RO, Brazil.Fundação Oswaldo Cruz. Departamento de Biologia. Laboratório de Entomologia Médica. Laboratório de Bioecologia de Insetos. Porto Velho, RO, Brazil.Universidades Federal de Lavras. Departamento de Química. Lavras, MG, Brazil.Universidade Federal de São João del Rei. Departamento de Química, Biotecnologia e Engenharia de Bioprocessos. Ouro Branco, MG, Brazil.Fundação Oswaldo Cruz. Centro de Estudos de Biomoléculas Aplicadas a Saúde. Departamento de Medicina. Universidade Federal de Rondônia. Porto Velho, RO, Brazil.Fundação Oswaldo Cruz. Centro de Estudos de Biomoléculas Aplicadas a Saúde. Departamento de Medicina. Universidade Federal de Rondônia. Porto Velho, RO, Brazil.Fundação Oswaldo Cruz. Centro de Estudos de Biomoléculas Aplicadas a Saúde. Departamento de Medicina. Universidade Federal de Rondônia. Porto Velho, RO, Brazil.Fundação Oswaldo Cruz. Centro de Estudos de Biomoléculas Aplicadas a Saúde. Departamento de Medicina. Universidade Federal de Rondônia. Porto Velho, RO, Brazil.Fundação Oswaldo Cruz. Centro de Estudos de Biomoléculas Aplicadas a Saúde. Departamento de Medicina. Universidade Federal de Rondônia. Porto Velho, RO, Brazil.Toxins purified from the venom of spiders have high potential to be studied pharmacologically and biochemically. These biomolecules may have biotechnological and therapeutic applications. This study aimed to evaluate the protein content of Parawixia bistriata venom and functionally characterize its proteins that have potential for biotechnological applications. The crude venom showed no phospholipase, hemorrhagic, or anti-Leishmania activities attesting to low genotoxicity and discrete antifungal activity for C. albicans. However the following activities were observed: anticoagulation, edema, myotoxicity and proteolysis on casein, azo-collagen, and fibrinogen. The chromatographic and electrophoretic profiles of the proteins revealed a predominance of acidic, neutral, and polar proteins, highlighting the presence of proteins with high molecular masses. Five fractions were collected using cation exchange chromatography, with the P4 fraction standing out as that of the highest purity. All fractions showed proteolytic activity. The crude venom and fractions P1, P2, and P3 showed larvicidal effects on A. aegypti. Fraction P4 showed the presence of a possible metalloprotease (60 kDa) that has high proteolytic activity on azo-collagen and was inhibited by EDTA. The results presented in this study demonstrate the presence of proteins in the venom of P. bistriata with potential for biotechnological applications.Parawixia bistriataSpider VenomPotential ProteolyticLarvicidal Activitiesinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/14300/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALBiochemical and Functional Characterization of Parawixia bistriata Spider Venom with Potential Proteolytic and Larvicidal Activities.pdfBiochemical and Functional Characterization of Parawixia bistriata Spider Venom with Potential Proteolytic and Larvicidal Activities.pdfapplication/pdf2345310https://www.arca.fiocruz.br/bitstream/icict/14300/2/Biochemical%20and%20Functional%20Characterization%20of%20Parawixia%20bistriata%20Spider%20Venom%20with%20Potential%20Proteolytic%20and%20Larvicidal%20Activities.pdf7b597bf6e451a2c576d5aa2c50136e25MD52TEXTBiochemical and Functional Characterization of Parawixia bistriata Spider Venom with Potential Proteolytic and Larvicidal Activities.pdf.txtBiochemical and Functional Characterization of Parawixia bistriata Spider Venom with Potential Proteolytic and Larvicidal Activities.pdf.txtExtracted texttext/plain60137https://www.arca.fiocruz.br/bitstream/icict/14300/3/Biochemical%20and%20Functional%20Characterization%20of%20Parawixia%20bistriata%20Spider%20Venom%20with%20Potential%20Proteolytic%20and%20Larvicidal%20Activities.pdf.txtedb0924b6824c8fa881dc19e76a31832MD53icict/143002018-04-02 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dc.title.pt_BR.fl_str_mv |
Biochemical and functional characterization of parawixia bistriata spider venom with potential proteolytic and larvicidal activities |
title |
Biochemical and functional characterization of parawixia bistriata spider venom with potential proteolytic and larvicidal activities |
spellingShingle |
Biochemical and functional characterization of parawixia bistriata spider venom with potential proteolytic and larvicidal activities Gimenez, Gizeli S. Parawixia bistriata Spider Venom Potential Proteolytic Larvicidal Activities |
title_short |
Biochemical and functional characterization of parawixia bistriata spider venom with potential proteolytic and larvicidal activities |
title_full |
Biochemical and functional characterization of parawixia bistriata spider venom with potential proteolytic and larvicidal activities |
title_fullStr |
Biochemical and functional characterization of parawixia bistriata spider venom with potential proteolytic and larvicidal activities |
title_full_unstemmed |
Biochemical and functional characterization of parawixia bistriata spider venom with potential proteolytic and larvicidal activities |
title_sort |
Biochemical and functional characterization of parawixia bistriata spider venom with potential proteolytic and larvicidal activities |
author |
Gimenez, Gizeli S. |
author_facet |
Gimenez, Gizeli S. Coutinho-Neto, Antonio Kayano, Anderson M. Silva, Rodrigo Simões Trindade, Frances Silva, Alexandre de Almeida e Marcussi, Silvana Silva, Saulo L. da Fernandes, Carla F. C. Zuliani, Juliana P. Calderon, Leonardo A. Soares, Andreimar M. Stábeli, Rodrigo G. |
author_role |
author |
author2 |
Coutinho-Neto, Antonio Kayano, Anderson M. Silva, Rodrigo Simões Trindade, Frances Silva, Alexandre de Almeida e Marcussi, Silvana Silva, Saulo L. da Fernandes, Carla F. C. Zuliani, Juliana P. Calderon, Leonardo A. Soares, Andreimar M. Stábeli, Rodrigo G. |
author2_role |
author author author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Gimenez, Gizeli S. Coutinho-Neto, Antonio Kayano, Anderson M. Silva, Rodrigo Simões Trindade, Frances Silva, Alexandre de Almeida e Marcussi, Silvana Silva, Saulo L. da Fernandes, Carla F. C. Zuliani, Juliana P. Calderon, Leonardo A. Soares, Andreimar M. Stábeli, Rodrigo G. |
dc.subject.en.pt_BR.fl_str_mv |
Parawixia bistriata Spider Venom Potential Proteolytic Larvicidal Activities |
topic |
Parawixia bistriata Spider Venom Potential Proteolytic Larvicidal Activities |
description |
Fundação Oswaldo Cruz. Centro de Estudos de Biomoléculas Aplicadas a Saúde. Departamento de Medicina. Universidade Federal de Rondônia. Porto Velho, RO, Brazil. |
publishDate |
2014 |
dc.date.issued.fl_str_mv |
2014 |
dc.date.accessioned.fl_str_mv |
2016-05-16T21:12:26Z |
dc.date.available.fl_str_mv |
2016-05-16T21:12:26Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
SOARES, A.M., et al. Biochemical and Functional Characterization of Parawixia bistriata Spider Venom with Potential Proteolytic and Larvicidal Activities. BioMed Research International, v. 2014, p. 1-13, 2014. |
dc.identifier.uri.fl_str_mv |
https://www.arca.fiocruz.br/handle/icict/14300 |
dc.identifier.doi.none.fl_str_mv |
http://dx.doi.org/10.1155/2014/950538 |
identifier_str_mv |
SOARES, A.M., et al. Biochemical and Functional Characterization of Parawixia bistriata Spider Venom with Potential Proteolytic and Larvicidal Activities. BioMed Research International, v. 2014, p. 1-13, 2014. |
url |
https://www.arca.fiocruz.br/handle/icict/14300 http://dx.doi.org/10.1155/2014/950538 |
dc.language.iso.fl_str_mv |
eng |
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eng |
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info:eu-repo/semantics/openAccess |
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openAccess |
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BioMed Research International |
publisher.none.fl_str_mv |
BioMed Research International |
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