Reversal of cerebrovascular constriction in experimental cerebral malaria by L-arginine

Detalhes bibliográficos
Autor(a) principal: Ong, Peng Kai
Data de Publicação: 2018
Outros Autores: Moreira, Aline S., Daniel-Ribeiro, Cláudio Tadeu, Frangos, John A., Carvalho, Leonardo J.M.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/31503
Resumo: La Jolla Bioengineering Institute. La Jolla, CA, USA.
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spelling Ong, Peng KaiMoreira, Aline S.Daniel-Ribeiro, Cláudio TadeuFrangos, John A.Carvalho, Leonardo J.M.2019-02-08T17:30:50Z2019-02-08T17:30:50Z2018ONG, Peng Kai et al. Reversal of cerebrovascular constriction in experimental cerebral malaria by L-arginine. Scientific Reports, v. 8, p. 1-11, 2018.2045-2322https://www.arca.fiocruz.br/handle/icict/3150310.1038/s41598-018-34249-2engNature ResearchReversãoConstrição cerebrovascularMalária cerebral experimentalL-argininaexperimental cerebral malariacerebrovascular constrictionL-arginineReversionReversal of cerebrovascular constriction in experimental cerebral malaria by L-arginineinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleLa Jolla Bioengineering Institute. La Jolla, CA, USA.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Malária. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Malária. Rio de Janeiro, RJ, Brasil.La Jolla Bioengineering Institute. La Jolla, CA, USA.La Jolla Bioengineering Institute. La Jolla, CA, USA / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Malária. Rio de Janeiro, RJ, Brasil.Vascular dysfunction associated with low nitric oxide (NO) biavailability and low plasma L-arginine levels is observed in both human and experimental cerebral malaria (ECM). In ECM, cerebrovascular constriction results in decreased pial blood flow and hypoxia, and administration of NO donors reverses constriction and increases survival. Supplementation of L-arginine, the substrate for NO synthesis by NO synthases, has been considered as a strategy to improve vascular health and act as adjunctive therapy in human severe malaria. We investigated the effect of L-arginine supplementation on pial vascular tonus of mice with ECM after direct superfusion on the brain surface or systemic delivery. Pial arteriolar diameters of Plasmodium berghei-infected mice with implanted cranial windows were measured using intravital microscopy methods, before and after L-arginine administration. Systemic delivery of L-arginine was performed intravenously, at 10, 50, 100 and 200 mg/kg, as bolus injection or slowly through osmotic pumps, combined or not with artesunate. Direct superfusion of L-arginine (10−7M, 10−5M and 10−3M) on the brain surface of mice with ECM resulted in immediate, consistent and dose-dependent dilation of pial arterioles. ECM mice showed marked cerebrovascular constriction that progressively worsened over a 24 h-period after subcutaneous saline bolus administration. L-arginine administration prevented the worsening in pial constriction at all the doses tested, and at 50 mg/kg and 100 mg/kg it induced temporary reversal of vasoconstriction. Slow, continuous delivery of L-arginine by osmotic pumps, or combined bolus administration of artesunate with L-arginine, also prevented worsening of pial constriction and resulted in improved survival of mice with ECM. 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dc.title.pt_BR.fl_str_mv Reversal of cerebrovascular constriction in experimental cerebral malaria by L-arginine
title Reversal of cerebrovascular constriction in experimental cerebral malaria by L-arginine
spellingShingle Reversal of cerebrovascular constriction in experimental cerebral malaria by L-arginine
Ong, Peng Kai
Reversão
Constrição cerebrovascular
Malária cerebral experimental
L-arginina
experimental cerebral malaria
cerebrovascular constriction
L-arginine
Reversion
title_short Reversal of cerebrovascular constriction in experimental cerebral malaria by L-arginine
title_full Reversal of cerebrovascular constriction in experimental cerebral malaria by L-arginine
title_fullStr Reversal of cerebrovascular constriction in experimental cerebral malaria by L-arginine
title_full_unstemmed Reversal of cerebrovascular constriction in experimental cerebral malaria by L-arginine
title_sort Reversal of cerebrovascular constriction in experimental cerebral malaria by L-arginine
author Ong, Peng Kai
author_facet Ong, Peng Kai
Moreira, Aline S.
Daniel-Ribeiro, Cláudio Tadeu
Frangos, John A.
Carvalho, Leonardo J.M.
author_role author
author2 Moreira, Aline S.
Daniel-Ribeiro, Cláudio Tadeu
Frangos, John A.
Carvalho, Leonardo J.M.
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Ong, Peng Kai
Moreira, Aline S.
Daniel-Ribeiro, Cláudio Tadeu
Frangos, John A.
Carvalho, Leonardo J.M.
dc.subject.other.pt_BR.fl_str_mv Reversão
Constrição cerebrovascular
Malária cerebral experimental
L-arginina
topic Reversão
Constrição cerebrovascular
Malária cerebral experimental
L-arginina
experimental cerebral malaria
cerebrovascular constriction
L-arginine
Reversion
dc.subject.en.pt_BR.fl_str_mv experimental cerebral malaria
cerebrovascular constriction
L-arginine
Reversion
description La Jolla Bioengineering Institute. La Jolla, CA, USA.
publishDate 2018
dc.date.issued.fl_str_mv 2018
dc.date.accessioned.fl_str_mv 2019-02-08T17:30:50Z
dc.date.available.fl_str_mv 2019-02-08T17:30:50Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv ONG, Peng Kai et al. Reversal of cerebrovascular constriction in experimental cerebral malaria by L-arginine. Scientific Reports, v. 8, p. 1-11, 2018.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/31503
dc.identifier.issn.pt_BR.fl_str_mv 2045-2322
dc.identifier.doi.none.fl_str_mv 10.1038/s41598-018-34249-2
identifier_str_mv ONG, Peng Kai et al. Reversal of cerebrovascular constriction in experimental cerebral malaria by L-arginine. Scientific Reports, v. 8, p. 1-11, 2018.
2045-2322
10.1038/s41598-018-34249-2
url https://www.arca.fiocruz.br/handle/icict/31503
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Nature Research
publisher.none.fl_str_mv Nature Research
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/31503/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/31503/2/claudioT_danielribeiro_etal_IOC_2018.pdf
https://www.arca.fiocruz.br/bitstream/icict/31503/3/claudioT_danielribeiro_etal_IOC_2018.pdf.txt
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repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
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