A bacterial artificial chromosome library for Biomphalaria glabrata, intermediate snail host of Schistosoma mansoni
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2006 |
| Outros Autores: | , , , , , , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/6489 |
Resumo: | University of New Mexico. Department of Biology. Albuquerque, NM, USA. |
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Adema, Coen M.Luo, Mei-ZhongHanelt, BenHertel, Lynn A.Marshall, Jennifer J.Si-Ming, ZhangDeJong, Randall J.Kim, Hye-RanKudrna, DavidWing, Rod A.Soderlund, CariKnight, MattyLewis, Fred A.Caldeira, Roberta LimaPassos, Liana Konovaloff JannottiCarvalho, Omar dos SantosLoker, Eric S.2013-05-14T13:50:34Z2013-05-14T13:50:34Z2006ADEMA, Coen Martinus et al. A bacterial artificial chromosome library for Biomphalaria glabrata, intermediate snail host of Schistosoma mansoni. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 101, Suppl. I, p. 167-177, 2006.0074-0276https://www.arca.fiocruz.br/handle/icict/6489engA bacterial artificial chromosome library for Biomphalaria glabrata, intermediate snail host of Schistosoma mansoniinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversity of New Mexico. Department of Biology. Albuquerque, NM, USA.Arizona Genomics Institute. Department of Plant Sciences.University of New Mexico. Department of Biology. ,Albuquerque, NM, USA.University of Arizona. BIO5 Institute. Arizona Genomics Computational Laboratory. Tucson, USA.University of Arizona. BIO5 Institute. Arizona Genomics Computational Laboratory. Tucson, USUniversity of Arizona. BIO5 Institute. Arizona Genomics Computational Laboratory. Tucson, USA.Arizona Genomics Institute. Department of Plant Sciences.Arizona Genomics Institute. Department of Plant SciencesArizona Genomics Institute. Department of Plant Sciences.University of Arizona. BIO5 Institute. Arizona Genomics Computational Laboratory. Tucson, USComputational Laboratory. Tucson, USA.Biomedical Research Institute. Rockville, MD, USA.Biomedical Research Institute. Rockville, MD, USA.Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Helmintoses Intestinais. Belo Horizonte, MG, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Helmintoses Intestinais. Belo Horizonte, MG, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Helmintoses Intestinais. Belo Horizonte, MG, Brasil.University of New Mexico. Department of Biology. Albuquerque, NM, USA.To provide a novel resource for analysis of the genome of Biomphalaria glabrata, members of the international Biomphalaria glabrata Genome Initiative (biology.unm.edu/biomphalaria-genome.html), working with the Arizona Genomics Institute (AGI) and supported by the National Human Genome Research Institute (NHGRI), produced a high quality bacterial artificial chromosome (BAC) library. The BB02 strain B. glabrata, a field isolate (Belo Horizonte, Minas Gerais, Brasil) that is susceptible to several strains of Schistosoma mansoni, was selfed for two generations to reduce haplotype diversity in the offspring. High molecular weight DNA was isolated from ovotestes of 40 snails, partially digested with HindIII, and ligated into pAGIBAC1 vector. The resulting B. glabrata BAC library(BG_BBa) consists of 61824 clones (136.3 kb average insert size) and provides 9.05 × coverage of the 931 Mb genome. Probing with single/low copy number genes from B. glabrata and fingerprinting of selected BAC clonesindicated that the BAC library sufficiently represents the gene complement. BAC end sequence data (514 reads,299860 nt) indicated that the genome of B. glabrata contains ~ 63% AT, and disclosed several novel genes, transposableelements, and groups of high frequency sequence elements. This BG_BBa BAC library, available from AGI atcost to the research community, gains in relevance because BB02 strain B. glabrata is targeted whole genome sequencing by NHGRI.GenomicsGene discoveryFingerprintingSchistosomiasisMedical malacologyinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALBacteria artificial X023.pdfBacteria artificial X023.pdfapplication/pdf852189https://www.arca.fiocruz.br/bitstream/icict/6489/1/Bacteria%20artificial%20X023.pdf81e4b4be4e2bfe68550a83e74b56e610MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://www.arca.fiocruz.br/bitstream/icict/6489/2/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD52TEXTBacteria artificial X023.pdf.txtBacteria artificial X023.pdf.txtExtracted texttext/plain54614https://www.arca.fiocruz.br/bitstream/icict/6489/5/Bacteria%20artificial%20X023.pdf.txt344b248e3f74ccd0cca798a7b2fbdefcMD55THUMBNAILBacteria artificial X023.pdf.jpgBacteria artificial X023.pdf.jpgGenerated Thumbnailimage/jpeg2239https://www.arca.fiocruz.br/bitstream/icict/6489/4/Bacteria%20artificial%20X023.pdf.jpg328ef81a063e6df8aae54afe1b53edffMD54icict/64892018-05-25 13:47:55.765oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352018-05-25T16:47:55Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false |
| dc.title.pt_BR.fl_str_mv |
A bacterial artificial chromosome library for Biomphalaria glabrata, intermediate snail host of Schistosoma mansoni |
| title |
A bacterial artificial chromosome library for Biomphalaria glabrata, intermediate snail host of Schistosoma mansoni |
| spellingShingle |
A bacterial artificial chromosome library for Biomphalaria glabrata, intermediate snail host of Schistosoma mansoni Adema, Coen M. Genomics Gene discovery Fingerprinting Schistosomiasis Medical malacology |
| title_short |
A bacterial artificial chromosome library for Biomphalaria glabrata, intermediate snail host of Schistosoma mansoni |
| title_full |
A bacterial artificial chromosome library for Biomphalaria glabrata, intermediate snail host of Schistosoma mansoni |
| title_fullStr |
A bacterial artificial chromosome library for Biomphalaria glabrata, intermediate snail host of Schistosoma mansoni |
| title_full_unstemmed |
A bacterial artificial chromosome library for Biomphalaria glabrata, intermediate snail host of Schistosoma mansoni |
| title_sort |
A bacterial artificial chromosome library for Biomphalaria glabrata, intermediate snail host of Schistosoma mansoni |
| author |
Adema, Coen M. |
| author_facet |
Adema, Coen M. Luo, Mei-Zhong Hanelt, Ben Hertel, Lynn A. Marshall, Jennifer J. Si-Ming, Zhang DeJong, Randall J. Kim, Hye-Ran Kudrna, David Wing, Rod A. Soderlund, Cari Knight, Matty Lewis, Fred A. Caldeira, Roberta Lima Passos, Liana Konovaloff Jannotti Carvalho, Omar dos Santos Loker, Eric S. |
| author_role |
author |
| author2 |
Luo, Mei-Zhong Hanelt, Ben Hertel, Lynn A. Marshall, Jennifer J. Si-Ming, Zhang DeJong, Randall J. Kim, Hye-Ran Kudrna, David Wing, Rod A. Soderlund, Cari Knight, Matty Lewis, Fred A. Caldeira, Roberta Lima Passos, Liana Konovaloff Jannotti Carvalho, Omar dos Santos Loker, Eric S. |
| author2_role |
author author author author author author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Adema, Coen M. Luo, Mei-Zhong Hanelt, Ben Hertel, Lynn A. Marshall, Jennifer J. Si-Ming, Zhang DeJong, Randall J. Kim, Hye-Ran Kudrna, David Wing, Rod A. Soderlund, Cari Knight, Matty Lewis, Fred A. Caldeira, Roberta Lima Passos, Liana Konovaloff Jannotti Carvalho, Omar dos Santos Loker, Eric S. |
| dc.subject.en.pt_BR.fl_str_mv |
Genomics Gene discovery Fingerprinting Schistosomiasis Medical malacology |
| topic |
Genomics Gene discovery Fingerprinting Schistosomiasis Medical malacology |
| description |
University of New Mexico. Department of Biology. Albuquerque, NM, USA. |
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2006 |
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2006 |
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2013-05-14T13:50:34Z |
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2013-05-14T13:50:34Z |
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ADEMA, Coen Martinus et al. A bacterial artificial chromosome library for Biomphalaria glabrata, intermediate snail host of Schistosoma mansoni. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 101, Suppl. I, p. 167-177, 2006. |
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https://www.arca.fiocruz.br/handle/icict/6489 |
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0074-0276 |
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ADEMA, Coen Martinus et al. A bacterial artificial chromosome library for Biomphalaria glabrata, intermediate snail host of Schistosoma mansoni. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 101, Suppl. I, p. 167-177, 2006. 0074-0276 |
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