Characterization of a novel trans-sialidase of Trypanosoma brucei procyclic trypomastigotes and identification of procyclin as the main sialic acid acceptor.

Pontes-de-Carvalho, Lain Carlos; Tomlinson, Stephen; Vandekerckhove, Filip; Bienen, E. Jay; Clarkson, S Allen B; Jiang, Man-Shiow; Hart, Gerald W; Nussenzweig, Victor

Characterization of a novel trans-sialidase of Trypanosoma brucei procyclic trypomastigotes and identification of procyclin as the main sialic acid acceptor.

Detalhes bibliográficos
Autor(a) principal:	Pontes-de-Carvalho, Lain Carlos
Data de Publicação:	1993
Outros Autores:	Tomlinson, Stephen, Vandekerckhove, Filip, Bienen, E. Jay, Clarkson, S Allen B, Jiang, Man-Shiow, Hart, Gerald W, Nussenzweig, Victor
Tipo de documento:	Artigo
Idioma:	eng
Título da fonte:	Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo:	https://www.arca.fiocruz.br/handle/icict/8338
Resumo:	New York University Medical Center. Department of Pathology and Kaplan Cancer Center. New York, New York / Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil

Metadados do item

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spelling	Pontes-de-Carvalho, Lain CarlosTomlinson, StephenVandekerckhove, FilipBienen, E. JayClarkson, S Allen BJiang, Man-ShiowHart, Gerald WNussenzweig, Victor2014-09-09T18:57:15Z2014-09-09T18:57:15Z1993PONTES-DE-CARVALHO, L.C. et al. Characterization of a novel trans-sialidase of Trypanosoma brucei procyclic trypomastigotes and identification of procyclin as the main sialic acid acceptor. Journal of Experimental Medicine, v. 177, n. 2, p. 465-474, 1993.0022-1007https://www.arca.fiocruz.br/handle/icict/8338engThe Rockefeller University PressCharacterization of a novel trans-sialidase of Trypanosoma brucei procyclic trypomastigotes and identification of procyclin as the main sialic acid acceptor.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleNew York University Medical Center. Department of Pathology and Kaplan Cancer Center. New York, New York / Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilNew York University Medical Center. Department of Pathology and Kaplan Cancer Center. New York, New YorkNew York University Medical Center. Department of Pathology and Kaplan Cancer Center. New York, New YorkNew York University Medical Center. Department of Medical and Molecular Parasitology. New York, New YorkNew York University Medical Center. Department of Medical and Molecular Parasitology. New York, New YorkThe Johns Hopkins University. Department of Biological Chemistry. Baltimore, MarylandThe Johns Hopkins University. Baltimore, MarylandNew York University Medical Center. Department of Pathology and Kaplan Cancer Center. New York, New YorkHere we report the presence of a trans-sialidase on the surface of Trypanosoma brucei culturederived procyclic trypomastigotes. The enzyme is not detected in lysates of bloodstream trypomastigotes enriched for either stumpy or slender forms. The trans-sialidase catalyzes the transfer of ot(2-3)-linked sialic acid residues to lactose./3-galactopyranosyl residues are at least 100 times better acceptors for sialic acid than ot-galactopyranosyl residues. In the absence of efficient acceptors, the purified enzyme transfers sialic acid to water, i.e., it acts as a sialidase. Although the T. cruzi and T. brucei trans-sialidases have very similar donor and acceptor specificities, they are antigenicaUy distinct. Sodium dodecyl sulfate--polyacramide gel electrophoresis under nonreducing conditions and silver staining of the purified trans-sialidase reveals a single band of 63 kD. When the surface membrane of live procyclic trypomastigotes is trans-sialylated, using radioactive sialyllactose as the donor substrate, it appears that the only sialylated surface molecule is procyclin. Pronase treatment of live parasites removes only part of the surface sialic acid, in agreement with recent data showing that the glycosylphosphatidylinositol anchor of procyclin is sialylated (Ferguson, M. A. J., M. Murray, H. Rutherford, and M. J. McConville. 1993. Biochem. J. In press).Glicoproteínas de MembranaSialiltransferases/metabolismoTrypanosoma brucei brucei/enzimologiaGlicoproteínas Variantes de Superfície de Trypanosoma/metabolismoAnimaisNeuraminidase/metabolismoProteínas de Protozoários/isolamento & purificaçãoProteínas de Protozoários/metabolismoÁcidos Siálicos/metabolismoSialoglicoproteínas/metabolismoSialiltransferases/isolamento & purificaçãoTrypanosoma brucei brucei/citologiaTrypanosoma cruzi/enzimologiainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://www.arca.fiocruz.br/bitstream/icict/8338/1/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD51ORIGINALPontes de Carvalho, LC Characterization of....pdfPontes de Carvalho, LC Characterization of....pdfapplication/pdf1051650https://www.arca.fiocruz.br/bitstream/icict/8338/2/Pontes%20de%20Carvalho%2c%20LC%20Characterization%20of....pdf6c49be4b312c5f9a0b10c8ee1aa61441MD52TEXTPontes de Carvalho, LC Characterization of....pdf.txtPontes de Carvalho, LC Characterization of....pdf.txtExtracted texttext/plain49070https://www.arca.fiocruz.br/bitstream/icict/8338/3/Pontes%20de%20Carvalho%2c%20LC%20Characterization%20of....pdf.txtd06e207c06be7adca1707598ccc248c5MD53icict/83382023-03-15 14:33:23.682oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-15T17:33:23Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv	Characterization of a novel trans-sialidase of Trypanosoma brucei procyclic trypomastigotes and identification of procyclin as the main sialic acid acceptor.
title	Characterization of a novel trans-sialidase of Trypanosoma brucei procyclic trypomastigotes and identification of procyclin as the main sialic acid acceptor.
spellingShingle	Characterization of a novel trans-sialidase of Trypanosoma brucei procyclic trypomastigotes and identification of procyclin as the main sialic acid acceptor. Pontes-de-Carvalho, Lain Carlos Glicoproteínas de Membrana Sialiltransferases/metabolismo Trypanosoma brucei brucei/enzimologia Glicoproteínas Variantes de Superfície de Trypanosoma/metabolismo Animais Neuraminidase/metabolismo Proteínas de Protozoários/isolamento & purificação Proteínas de Protozoários/metabolismo Ácidos Siálicos/metabolismo Sialoglicoproteínas/metabolismo Sialiltransferases/isolamento & purificação Trypanosoma brucei brucei/citologia Trypanosoma cruzi/enzimologia
title_short	Characterization of a novel trans-sialidase of Trypanosoma brucei procyclic trypomastigotes and identification of procyclin as the main sialic acid acceptor.
title_full	Characterization of a novel trans-sialidase of Trypanosoma brucei procyclic trypomastigotes and identification of procyclin as the main sialic acid acceptor.
title_fullStr	Characterization of a novel trans-sialidase of Trypanosoma brucei procyclic trypomastigotes and identification of procyclin as the main sialic acid acceptor.
title_full_unstemmed	Characterization of a novel trans-sialidase of Trypanosoma brucei procyclic trypomastigotes and identification of procyclin as the main sialic acid acceptor.
title_sort	Characterization of a novel trans-sialidase of Trypanosoma brucei procyclic trypomastigotes and identification of procyclin as the main sialic acid acceptor.
author	Pontes-de-Carvalho, Lain Carlos
author_facet	Pontes-de-Carvalho, Lain Carlos Tomlinson, Stephen Vandekerckhove, Filip Bienen, E. Jay Clarkson, S Allen B Jiang, Man-Shiow Hart, Gerald W Nussenzweig, Victor
author_role	author
author2	Tomlinson, Stephen Vandekerckhove, Filip Bienen, E. Jay Clarkson, S Allen B Jiang, Man-Shiow Hart, Gerald W Nussenzweig, Victor
author2_role	author author author author author author author
dc.contributor.author.fl_str_mv	Pontes-de-Carvalho, Lain Carlos Tomlinson, Stephen Vandekerckhove, Filip Bienen, E. Jay Clarkson, S Allen B Jiang, Man-Shiow Hart, Gerald W Nussenzweig, Victor
dc.subject.decs.pt_BR.fl_str_mv	Glicoproteínas de Membrana Sialiltransferases/metabolismo Trypanosoma brucei brucei/enzimologia Glicoproteínas Variantes de Superfície de Trypanosoma/metabolismo Animais Neuraminidase/metabolismo Proteínas de Protozoários/isolamento & purificação Proteínas de Protozoários/metabolismo Ácidos Siálicos/metabolismo Sialoglicoproteínas/metabolismo Sialiltransferases/isolamento & purificação Trypanosoma brucei brucei/citologia Trypanosoma cruzi/enzimologia
topic	Glicoproteínas de Membrana Sialiltransferases/metabolismo Trypanosoma brucei brucei/enzimologia Glicoproteínas Variantes de Superfície de Trypanosoma/metabolismo Animais Neuraminidase/metabolismo Proteínas de Protozoários/isolamento & purificação Proteínas de Protozoários/metabolismo Ácidos Siálicos/metabolismo Sialoglicoproteínas/metabolismo Sialiltransferases/isolamento & purificação Trypanosoma brucei brucei/citologia Trypanosoma cruzi/enzimologia
description	New York University Medical Center. Department of Pathology and Kaplan Cancer Center. New York, New York / Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil
publishDate	1993
dc.date.issued.fl_str_mv	1993
dc.date.accessioned.fl_str_mv	2014-09-09T18:57:15Z
dc.date.available.fl_str_mv	2014-09-09T18:57:15Z
dc.type.status.fl_str_mv	info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv	info:eu-repo/semantics/article
format	article
status_str	publishedVersion
dc.identifier.citation.fl_str_mv	PONTES-DE-CARVALHO, L.C. et al. Characterization of a novel trans-sialidase of Trypanosoma brucei procyclic trypomastigotes and identification of procyclin as the main sialic acid acceptor. Journal of Experimental Medicine, v. 177, n. 2, p. 465-474, 1993.
dc.identifier.uri.fl_str_mv	https://www.arca.fiocruz.br/handle/icict/8338
dc.identifier.issn.none.fl_str_mv	0022-1007
identifier_str_mv	PONTES-DE-CARVALHO, L.C. et al. Characterization of a novel trans-sialidase of Trypanosoma brucei procyclic trypomastigotes and identification of procyclin as the main sialic acid acceptor. Journal of Experimental Medicine, v. 177, n. 2, p. 465-474, 1993. 0022-1007
url	https://www.arca.fiocruz.br/handle/icict/8338
dc.language.iso.fl_str_mv	eng
language	eng
dc.rights.driver.fl_str_mv	info:eu-repo/semantics/openAccess
eu_rights_str_mv	openAccess
dc.publisher.none.fl_str_mv	The Rockefeller University Press
publisher.none.fl_str_mv	The Rockefeller University Press
dc.source.none.fl_str_mv	reponame:Repositório Institucional da FIOCRUZ (ARCA) instname:Fundação Oswaldo Cruz (FIOCRUZ) instacron:FIOCRUZ
instname_str	Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str	FIOCRUZ
institution	FIOCRUZ
reponame_str	Repositório Institucional da FIOCRUZ (ARCA)
collection	Repositório Institucional da FIOCRUZ (ARCA)
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Characterization of a novel trans-sialidase of Trypanosoma brucei procyclic trypomastigotes and identification of procyclin as the main sialic acid acceptor.

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