Biological and Genetic Heterogeneity in Trypanosoma dionisii Isolates from Hematophagous and Insectivorous Bats
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2020 |
| Outros Autores: | , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/45832 |
Resumo: | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia de Tripanosomatídeos. Rio de Janeiro, RJ, Brasil. |
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Barros, Juliana Helena da SilvaRoque, André Luiz RodriguesXavier, Samanta Cristina das ChagasNascimento, Kátia Cristina SilvaToma, Helena KeikoMadeira, Maria de Fatima2021-01-26T18:31:04Z2021-01-26T18:31:04Z2020BARROS, Juliana Helena da Silva et al. Biological and Genetic Heterogeneity in Trypanosoma dionisii Isolates from Hematophagous and Insectivorous Bats. Pathogens, v. 9, n. 736, 12p, 2020.2076-0817https://www.arca.fiocruz.br/handle/icict/4583210.3390/pathogens9090736engMDPIChiropteraSchizotrypanumCaracterização molecularMetodologias integrativasCaracterização biológicaDiferences entre isoladosTrypanosomatinaTrypanosoma cruziChiropteraTrypanosomatidaeSchizotrypanumIntegrative methodologiesMolecular characterizationBiological characterizationDiferences among isolatesBiological and Genetic Heterogeneity in Trypanosoma dionisii Isolates from Hematophagous and Insectivorous Batsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia de Tripanosomatídeos. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia de Tripanosomatídeos. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia de Tripanosomatídeos. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia de Tripanosomatídeos. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Faculdade de Farmácia. Laboratório de Diagnóstico Molecular e Hematologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Vigilância em Leishmanioses. Rio de Janeiro, RJ, Brasil.This study describes the morphological, biochemical, and molecular di erences among Trypanosoma dionisii isolates from hemocultures of hematophagous (Desmodus rotundus; n = 2) and insectivorous (Lonchorhina aurita; n = 1) bats from the Atlantic Rainforest of Rio de Janeiro, Brazil. Fusiform epimastigotes from the hematophagous isolates were elongated, whereas those of the insectivorous isolate were stumpy, reflected in statistically evident di erences in the cell body and flagellum lengths. In the hemocultures, a higher percentage of trypomastigote forms (60%) was observed in the hematophagous bat isolates than that in the isolate from the insectivorous bat (4%), which demonstrated globular morphology. Three molecular DNA regions were analyzed: V7V8 (18S rDNA), glycosomal glyceraldehyde 3-phosphate dehydrogenase gene, and mitochondrial cytochrome b gene. The samples were also subjected to multilocus enzyme electrophoresis and random amplified polymorphic DNA analysis. All isolates were identified as T. dionisii by phylogenetic analysis. These sequences were clustered into two separate subgroups with high bootstrap values according to the feeding habits of the bats from which the parasites were isolated. However, other T. dionisii samples from bats with di erent feeding habits were found in the same branch. These results support the separation of the three isolates into two subgroups, demonstrating that di erent subpopulations of T. dionisii circulate among bats.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/45832/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALSamantaXavier_JulianaBarros_etal_IOC_2020.pdfSamantaXavier_JulianaBarros_etal_IOC_2020.pdfapplication/pdf1037488https://www.arca.fiocruz.br/bitstream/icict/45832/2/SamantaXavier_JulianaBarros_etal_IOC_2020.pdfe8ed4b034a708a48a736949151eb8a55MD52TEXTSamantaXavier_JulianaBarros_etal_IOC_2020.pdf.txtSamantaXavier_JulianaBarros_etal_IOC_2020.pdf.txtExtracted texttext/plain48270https://www.arca.fiocruz.br/bitstream/icict/45832/3/SamantaXavier_JulianaBarros_etal_IOC_2020.pdf.txta9178c0e30f8e11b2b8515d24306690dMD53icict/458322021-01-27 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| dc.title.pt_BR.fl_str_mv |
Biological and Genetic Heterogeneity in Trypanosoma dionisii Isolates from Hematophagous and Insectivorous Bats |
| title |
Biological and Genetic Heterogeneity in Trypanosoma dionisii Isolates from Hematophagous and Insectivorous Bats |
| spellingShingle |
Biological and Genetic Heterogeneity in Trypanosoma dionisii Isolates from Hematophagous and Insectivorous Bats Barros, Juliana Helena da Silva Chiroptera Schizotrypanum Caracterização molecular Metodologias integrativas Caracterização biológica Diferences entre isolados Trypanosomatina Trypanosoma cruzi Chiroptera Trypanosomatidae Schizotrypanum Integrative methodologies Molecular characterization Biological characterization Diferences among isolates |
| title_short |
Biological and Genetic Heterogeneity in Trypanosoma dionisii Isolates from Hematophagous and Insectivorous Bats |
| title_full |
Biological and Genetic Heterogeneity in Trypanosoma dionisii Isolates from Hematophagous and Insectivorous Bats |
| title_fullStr |
Biological and Genetic Heterogeneity in Trypanosoma dionisii Isolates from Hematophagous and Insectivorous Bats |
| title_full_unstemmed |
Biological and Genetic Heterogeneity in Trypanosoma dionisii Isolates from Hematophagous and Insectivorous Bats |
| title_sort |
Biological and Genetic Heterogeneity in Trypanosoma dionisii Isolates from Hematophagous and Insectivorous Bats |
| author |
Barros, Juliana Helena da Silva |
| author_facet |
Barros, Juliana Helena da Silva Roque, André Luiz Rodrigues Xavier, Samanta Cristina das Chagas Nascimento, Kátia Cristina Silva Toma, Helena Keiko Madeira, Maria de Fatima |
| author_role |
author |
| author2 |
Roque, André Luiz Rodrigues Xavier, Samanta Cristina das Chagas Nascimento, Kátia Cristina Silva Toma, Helena Keiko Madeira, Maria de Fatima |
| author2_role |
author author author author author |
| dc.contributor.author.fl_str_mv |
Barros, Juliana Helena da Silva Roque, André Luiz Rodrigues Xavier, Samanta Cristina das Chagas Nascimento, Kátia Cristina Silva Toma, Helena Keiko Madeira, Maria de Fatima |
| dc.subject.other.pt_BR.fl_str_mv |
Chiroptera Schizotrypanum Caracterização molecular Metodologias integrativas Caracterização biológica Diferences entre isolados Trypanosomatina Trypanosoma cruzi |
| topic |
Chiroptera Schizotrypanum Caracterização molecular Metodologias integrativas Caracterização biológica Diferences entre isolados Trypanosomatina Trypanosoma cruzi Chiroptera Trypanosomatidae Schizotrypanum Integrative methodologies Molecular characterization Biological characterization Diferences among isolates |
| dc.subject.en.pt_BR.fl_str_mv |
Chiroptera Trypanosomatidae Schizotrypanum Integrative methodologies Molecular characterization Biological characterization Diferences among isolates |
| description |
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia de Tripanosomatídeos. Rio de Janeiro, RJ, Brasil. |
| publishDate |
2020 |
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2020 |
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2021-01-26T18:31:04Z |
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2021-01-26T18:31:04Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
| dc.identifier.citation.fl_str_mv |
BARROS, Juliana Helena da Silva et al. Biological and Genetic Heterogeneity in Trypanosoma dionisii Isolates from Hematophagous and Insectivorous Bats. Pathogens, v. 9, n. 736, 12p, 2020. |
| dc.identifier.uri.fl_str_mv |
https://www.arca.fiocruz.br/handle/icict/45832 |
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2076-0817 |
| dc.identifier.doi.none.fl_str_mv |
10.3390/pathogens9090736 |
| identifier_str_mv |
BARROS, Juliana Helena da Silva et al. Biological and Genetic Heterogeneity in Trypanosoma dionisii Isolates from Hematophagous and Insectivorous Bats. Pathogens, v. 9, n. 736, 12p, 2020. 2076-0817 10.3390/pathogens9090736 |
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MDPI |
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MDPI |
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