STAT3 gene expression in ameloblastomas and odontogenic keratocysts

Detalhes bibliográficos
Autor(a) principal: Melo, Leonardo de Araújo
Data de Publicação: 2022
Outros Autores: Santana, Dandara Andrade de, Sales, Caroline Brandi Schlaepfer, Carneiro Júnior, Bráulio, Martins, Marília Trierveiler, Freitas, Valéria Souza, Xavier, Flávia Caló Aquino, Cury, Patrícia Ramos, Gurgel, Clarissa Araújo Silva, Santos, Jean Nunes dos
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/57050
Resumo: Conselho Nacional de Desenvolvimento Científico e Tecnológico Desenvolvimento (CNPq). Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES).
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spelling Melo, Leonardo de AraújoSantana, Dandara Andrade deSales, Caroline Brandi SchlaepferCarneiro Júnior, BráulioMartins, Marília TrierveilerFreitas, Valéria SouzaXavier, Flávia Caló AquinoCury, Patrícia RamosGurgel, Clarissa Araújo SilvaSantos, Jean Nunes dos2023-02-13T18:37:10Z2023-02-13T18:37:10Z2022MELO, Leonardo de Araújo et al. STAT3 gene expression in ameloblastomas and odontogenic keratocyst. Srpski Arhiv za Celokupno Lekarstvo, p. 1-16, 2022.2406-0895https://www.arca.fiocruz.br/handle/icict/5705010.2298/SARH220607115AConselho Nacional de Desenvolvimento Científico e Tecnológico Desenvolvimento (CNPq). Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES).Universidade Federal da Bahia. Faculdade de Odontologia. Laboratório de Patologia Cirúrgica. Salvador, BA, Brasil.Universidade Federal da Bahia. Programa Odontologia e Saúde. Salvador, BA, Brasil.Universidade Federal da Bahia. Departamento de Biomorfologia. Salvador, BA, Brasil.Universidade do Sul da Bahia. Departamento de Cirurgia Bucomaxilofacial. Jequié, BA, Brasil.Universidade de São Paulo. Departamento de Patologia Bucomaxilofacial. São Paulo, SP, Brasil.Universidade Estadual de Feira de Santana. Secretaria de Saúde. Feira de Santana, BA, Brasil.Universidade Federal da Bahia. Faculdade de Odontologia. Laboratório de Patologia Cirúrgica. Salvador, BA, Brasil.Universidade Federal da Bahia. Programa Odontologia e Saúde. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Muniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Programa Odontologia e Saúde. Salvador, BA, Brasil.Universidade Federal da Bahia. Faculdade de Odontologia. Laboratório de Patologia Cirúrgica. Salvador, BA, Brasil / Universidade Federal da Bahia. Programa Odontologia e Saúde. Salvador, BA, Brasil.Introduction/Objective. STAT3 (signal transducers and activators of transcription) is involved in different physiological processes, including cell proliferation and survival. High expression of this protein is observed in various types of cancer. This study aimed to investigate the gene and protein expression of STAT3 in a series of odontogenic cysts and tumors to provide more information about their biological profile. Methods. The STAT3 gene expression at mRNA was quantified by real-time quantitative polymerase chain reaction (RT-qPCR) in 23 odontogenic keratocysts (OKCs) and 7 ameloblastomas (AMs), and compared to the non-neoplastic oral mucosa. We also assessed the expression of STAT3 gene at protein levels, using immunohistochemistry, in 43 OKCs and 47 AMs. Results. STAT3 transcripts were found in 96.6% of the tumors studied; however, the gene was downregulated in OKC and AM compared to the non-neoplastic oral mucosa. The STAT3 gene expression at mRNA level was higher in sporadic OKC than syndromic OKC (p = 0.04). There was no difference in STAT3 gene expression at mRNA level between OKCs and AMs (p = 0.88). Immunostaining of STAT3 revealed no significant difference between sporadic and syndrome OKC (p > 0.05), nor between conventional and unicystic AMs (p > 0.05). Ameloblastomas exhibited significantly higher STAT3 immunostaining than OKCs (p = 0.03). In OKC and AM, STAT3 immunostaining was predominantly cytoplasmic and no difference in the cellular localization of STAT3 was observed between these lesions (p = 0.58). Conclusion. Our findings showed low expression of STAT3 gene in OKCs and AMs in relation to non-neoplastic oral mucosa. However, higher STAT3 immunostaining was observed in AMs compared to OKCs.engSrpski Lekarski DrustvoCistos odontogênicosAmeloblastomaFator de transcrição STAT3Expressão genéticaImuno-histoquímicaOdontogenic cystsAmeloblastomaSTAT3 transcription factorGene expressionImmunohistochemistryCistos odontogênicosAmeloblastomaFator de Transcrição STAT3GenéticaImuno-histoquímicaSTAT3 gene expression in ameloblastomas and odontogenic keratocystsЕкспресија гена STAT3 код амелобластома и одонтогених кератоцистаinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/57050/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALMelo, L.A. Stat3 gene expression in ameoblastomas...2022.pdfMelo, L.A. Stat3 gene expression in ameoblastomas...2022.pdfapplication/pdf1024479https://www.arca.fiocruz.br/bitstream/icict/57050/2/Melo%2c%20L.A.%20Stat3%20gene%20expression%20in%20ameoblastomas...2022.pdf9874d76beeb775c744843246e82bdc13MD52icict/570502023-03-15 14:32:52.432oai:www.arca.fiocruz.br:icict/57050Q0VTU8ODTyBOw4NPIEVYQ0xVU0lWQSBERSBESVJFSVRPUyBBVVRPUkFJUwoKQW8gYWNlaXRhciBvcyBURVJNT1MgZSBDT05EScOHw5VFUyBkZXN0YSBDRVNTw4NPLCBvIEFVVE9SIGUvb3UgVElUVUxBUiBkZSBkaXJlaXRvcwphdXRvcmFpcyBzb2JyZSBhIE9CUkEgZGUgcXVlIHRyYXRhIGVzdGUgZG9jdW1lbnRvOgoKKDEpIENFREUgZSBUUkFOU0ZFUkUsIHRvdGFsIGUgZ3JhdHVpdGFtZW50ZSwgw6AgRklPQ1JVWiAtIEZVTkRBw4fDg08gT1NXQUxETyBDUlVaLCBlbQpjYXLDoXRlciBwZXJtYW5lbnRlLCBpcnJldm9nw6F2ZWwgZSBOw4NPIEVYQ0xVU0lWTywgdG9kb3Mgb3MgZGlyZWl0b3MgcGF0cmltb25pYWlzIE7Dg08KQ09NRVJDSUFJUyBkZSB1dGlsaXphw6fDo28gZGEgT0JSQSBhcnTDrXN0aWNhIGUvb3UgY2llbnTDrWZpY2EgaW5kaWNhZGEgYWNpbWEsIGluY2x1c2l2ZSBvcyBkaXJlaXRvcwpkZSB2b3ogZSBpbWFnZW0gdmluY3VsYWRvcyDDoCBPQlJBLCBkdXJhbnRlIHRvZG8gbyBwcmF6byBkZSBkdXJhw6fDo28gZG9zIGRpcmVpdG9zIGF1dG9yYWlzLCBlbQpxdWFscXVlciBpZGlvbWEgZSBlbSB0b2RvcyBvcyBwYcOtc2VzOwoKKDIpIEFDRUlUQSBxdWUgYSBjZXNzw6NvIHRvdGFsIG7Do28gZXhjbHVzaXZhLCBwZXJtYW5lbnRlIGUgaXJyZXZvZ8OhdmVsIGRvcyBkaXJlaXRvcyBhdXRvcmFpcwpwYXRyaW1vbmlhaXMgbsOjbyBjb21lcmNpYWlzIGRlIHV0aWxpemHDp8OjbyBkZSBxdWUgdHJhdGEgZXN0ZSBkb2N1bWVudG8gaW5jbHVpLCBleGVtcGxpZmljYXRpdmFtZW50ZSwKb3MgZGlyZWl0b3MgZGUgZGlzcG9uaWJpbGl6YcOnw6NvIGUgY29tdW5pY2HDp8OjbyBww7pibGljYSBkYSBPQlJBLCBlbSBxdWFscXVlciBtZWlvIG91IHZlw61jdWxvLAppbmNsdXNpdmUgZW0gUmVwb3NpdMOzcmlvcyBEaWdpdGFpcywgYmVtIGNvbW8gb3MgZGlyZWl0b3MgZGUgcmVwcm9kdcOnw6NvLCBleGliacOnw6NvLCBleGVjdcOnw6NvLApkZWNsYW1hw6fDo28sIHJlY2l0YcOnw6NvLCBleHBvc2nDp8OjbywgYXJxdWl2YW1lbnRvLCBpbmNsdXPDo28gZW0gYmFuY28gZGUgZGFkb3MsIHByZXNlcnZhw6fDo28sIGRpZnVzw6NvLApkaXN0cmlidWnDp8OjbywgZGl2dWxnYcOnw6NvLCBlbXByw6lzdGltbywgdHJhZHXDp8OjbywgZHVibGFnZW0sIGxlZ2VuZGFnZW0sIGluY2x1c8OjbyBlbSBub3ZhcyBvYnJhcyBvdQpjb2xldMOibmVhcywgcmV1dGlsaXphw6fDo28sIGVkacOnw6NvLCBwcm9kdcOnw6NvIGRlIG1hdGVyaWFsIGRpZMOhdGljbyBlIGN1cnNvcyBvdSBxdWFscXVlciBmb3JtYSBkZQp1dGlsaXphw6fDo28gbsOjbyBjb21lcmNpYWw7CgooMykgUkVDT05IRUNFIHF1ZSBhIGNlc3PDo28gYXF1aSBlc3BlY2lmaWNhZGEgY29uY2VkZSDDoCBGSU9DUlVaIC0gRlVOREHDh8ODTyBPU1dBTERPCkNSVVogbyBkaXJlaXRvIGRlIGF1dG9yaXphciBxdWFscXVlciBwZXNzb2Eg4oCTIGbDrXNpY2Egb3UganVyw61kaWNhLCBww7pibGljYSBvdSBwcml2YWRhLCBuYWNpb25hbCBvdQplc3RyYW5nZWlyYSDigJMgYSBhY2Vzc2FyIGUgdXRpbGl6YXIgYW1wbGFtZW50ZSBhIE9CUkEsIHNlbSBleGNsdXNpdmlkYWRlLCBwYXJhIHF1YWlzcXVlcgpmaW5hbGlkYWRlcyBuw6NvIGNvbWVyY2lhaXM7CgooNCkgREVDTEFSQSBxdWUgYSBvYnJhIMOpIGNyaWHDp8OjbyBvcmlnaW5hbCBlIHF1ZSDDqSBvIHRpdHVsYXIgZG9zIGRpcmVpdG9zIGFxdWkgY2VkaWRvcyBlIGF1dG9yaXphZG9zLApyZXNwb25zYWJpbGl6YW5kby1zZSBpbnRlZ3JhbG1lbnRlIHBlbG8gY29udGXDumRvIGUgb3V0cm9zIGVsZW1lbnRvcyBxdWUgZmF6ZW0gcGFydGUgZGEgT0JSQSwKaW5jbHVzaXZlIG9zIGRpcmVpdG9zIGRlIHZveiBlIGltYWdlbSB2aW5jdWxhZG9zIMOgIE9CUkEsIG9icmlnYW5kby1zZSBhIGluZGVuaXphciB0ZXJjZWlyb3MgcG9yCmRhbm9zLCBiZW0gY29tbyBpbmRlbml6YXIgZSByZXNzYXJjaXIgYSBGSU9DUlVaIC0gRlVOREHDh8ODTyBPU1dBTERPIENSVVogZGUKZXZlbnR1YWlzIGRlc3Blc2FzIHF1ZSB2aWVyZW0gYSBzdXBvcnRhciwgZW0gcmF6w6NvIGRlIHF1YWxxdWVyIG9mZW5zYSBhIGRpcmVpdG9zIGF1dG9yYWlzIG91CmRpcmVpdG9zIGRlIHZveiBvdSBpbWFnZW0sIHByaW5jaXBhbG1lbnRlIG5vIHF1ZSBkaXogcmVzcGVpdG8gYSBwbMOhZ2lvIGUgdmlvbGHDp8O1ZXMgZGUgZGlyZWl0b3M7CgooNSkgQUZJUk1BIHF1ZSBjb25oZWNlIGEgUG9sw610aWNhIEluc3RpdHVjaW9uYWwgZGUgQWNlc3NvIEFiZXJ0byBkYSBGSU9DUlVaIC0gRlVOREHDh8ODTwpPU1dBTERPIENSVVogZSBhcyBkaXJldHJpemVzIHBhcmEgbyBmdW5jaW9uYW1lbnRvIGRvIHJlcG9zaXTDs3JpbyBpbnN0aXR1Y2lvbmFsIEFSQ0EuCgpBIFBvbMOtdGljYSBJbnN0aXR1Y2lvbmFsIGRlIEFjZXNzbyBBYmVydG8gZGEgRklPQ1JVWiAtIEZVTkRBw4fDg08gT1NXQUxETyBDUlVaIHJlc2VydmEKZXhjbHVzaXZhbWVudGUgYW8gQVVUT1Igb3MgZGlyZWl0b3MgbW9yYWlzIGUgb3MgdXNvcyBjb21lcmNpYWlzIHNvYnJlIGFzIG9icmFzIGRlIHN1YSBhdXRvcmlhCmUvb3UgdGl0dWxhcmlkYWRlLCBzZW5kbyBvcyB0ZXJjZWlyb3MgdXN1w6FyaW9zIHJlc3BvbnPDoXZlaXMgcGVsYSBhdHJpYnVpw6fDo28gZGUgYXV0b3JpYSBlIG1hbnV0ZW7Dp8OjbwpkYSBpbnRlZ3JpZGFkZSBkYSBPQlJBIGVtIHF1YWxxdWVyIHV0aWxpemHDp8Ojby4KCkEgUG9sw610aWNhIEluc3RpdHVjaW9uYWwgZGUgQWNlc3NvIEFiZXJ0byBkYSBGSU9DUlVaIC0gRlVOREHDh8ODTyBPU1dBTERPIENSVVoKcmVzcGVpdGEgb3MgY29udHJhdG9zIGUgYWNvcmRvcyBwcmVleGlzdGVudGVzIGRvcyBBdXRvcmVzIGNvbSB0ZXJjZWlyb3MsIGNhYmVuZG8gYW9zIEF1dG9yZXMKaW5mb3JtYXIgw6AgSW5zdGl0dWnDp8OjbyBhcyBjb25kacOnw7VlcyBlIG91dHJhcyByZXN0cmnDp8O1ZXMgaW1wb3N0YXMgcG9yIGVzdGVzIGluc3RydW1lbnRvcy4KRepositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-15T17:32:52Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.en_US.fl_str_mv STAT3 gene expression in ameloblastomas and odontogenic keratocysts
dc.title.alternative.en_US.fl_str_mv Експресија гена STAT3 код амелобластома и одонтогених кератоциста
title STAT3 gene expression in ameloblastomas and odontogenic keratocysts
spellingShingle STAT3 gene expression in ameloblastomas and odontogenic keratocysts
Melo, Leonardo de Araújo
Cistos odontogênicos
Ameloblastoma
Fator de transcrição STAT3
Expressão genética
Imuno-histoquímica
Odontogenic cysts
Ameloblastoma
STAT3 transcription factor
Gene expression
Immunohistochemistry
Cistos odontogênicos
Ameloblastoma
Fator de Transcrição STAT3
Genética
Imuno-histoquímica
title_short STAT3 gene expression in ameloblastomas and odontogenic keratocysts
title_full STAT3 gene expression in ameloblastomas and odontogenic keratocysts
title_fullStr STAT3 gene expression in ameloblastomas and odontogenic keratocysts
title_full_unstemmed STAT3 gene expression in ameloblastomas and odontogenic keratocysts
title_sort STAT3 gene expression in ameloblastomas and odontogenic keratocysts
author Melo, Leonardo de Araújo
author_facet Melo, Leonardo de Araújo
Santana, Dandara Andrade de
Sales, Caroline Brandi Schlaepfer
Carneiro Júnior, Bráulio
Martins, Marília Trierveiler
Freitas, Valéria Souza
Xavier, Flávia Caló Aquino
Cury, Patrícia Ramos
Gurgel, Clarissa Araújo Silva
Santos, Jean Nunes dos
author_role author
author2 Santana, Dandara Andrade de
Sales, Caroline Brandi Schlaepfer
Carneiro Júnior, Bráulio
Martins, Marília Trierveiler
Freitas, Valéria Souza
Xavier, Flávia Caló Aquino
Cury, Patrícia Ramos
Gurgel, Clarissa Araújo Silva
Santos, Jean Nunes dos
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Melo, Leonardo de Araújo
Santana, Dandara Andrade de
Sales, Caroline Brandi Schlaepfer
Carneiro Júnior, Bráulio
Martins, Marília Trierveiler
Freitas, Valéria Souza
Xavier, Flávia Caló Aquino
Cury, Patrícia Ramos
Gurgel, Clarissa Araújo Silva
Santos, Jean Nunes dos
dc.subject.other.en_US.fl_str_mv Cistos odontogênicos
Ameloblastoma
Fator de transcrição STAT3
Expressão genética
Imuno-histoquímica
topic Cistos odontogênicos
Ameloblastoma
Fator de transcrição STAT3
Expressão genética
Imuno-histoquímica
Odontogenic cysts
Ameloblastoma
STAT3 transcription factor
Gene expression
Immunohistochemistry
Cistos odontogênicos
Ameloblastoma
Fator de Transcrição STAT3
Genética
Imuno-histoquímica
dc.subject.en.en_US.fl_str_mv Odontogenic cysts
Ameloblastoma
STAT3 transcription factor
Gene expression
Immunohistochemistry
dc.subject.decs.en_US.fl_str_mv Cistos odontogênicos
Ameloblastoma
Fator de Transcrição STAT3
Genética
Imuno-histoquímica
description Conselho Nacional de Desenvolvimento Científico e Tecnológico Desenvolvimento (CNPq). Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES).
publishDate 2022
dc.date.issued.fl_str_mv 2022
dc.date.accessioned.fl_str_mv 2023-02-13T18:37:10Z
dc.date.available.fl_str_mv 2023-02-13T18:37:10Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.citation.fl_str_mv MELO, Leonardo de Araújo et al. STAT3 gene expression in ameloblastomas and odontogenic keratocyst. Srpski Arhiv za Celokupno Lekarstvo, p. 1-16, 2022.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/57050
dc.identifier.issn.en_US.fl_str_mv 2406-0895
dc.identifier.doi.none.fl_str_mv 10.2298/SARH220607115A
identifier_str_mv MELO, Leonardo de Araújo et al. STAT3 gene expression in ameloblastomas and odontogenic keratocyst. Srpski Arhiv za Celokupno Lekarstvo, p. 1-16, 2022.
2406-0895
10.2298/SARH220607115A
url https://www.arca.fiocruz.br/handle/icict/57050
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Srpski Lekarski Drustvo
publisher.none.fl_str_mv Srpski Lekarski Drustvo
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/57050/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/57050/2/Melo%2c%20L.A.%20Stat3%20gene%20expression%20in%20ameoblastomas...2022.pdf
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