High-Throughput Method for Detection of Arbovirus Infection of Saliva in Mosquitoes Aedes aegypti and Ae. albopictus

Detalhes bibliográficos
Autor(a) principal: Honório, Nildimar Alves
Data de Publicação: 2020
Outros Autores: Câmara, Daniel Cardoso Portela, Wiggins, Keenan Wiggins, Eastmond, Bradley, Alto, Barry Wilmer
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/45527
Resumo: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Mosquitos Transmissores de Hematozoários. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Núcleo Operacional Sentinela de Mosquitos Vetores-Nosmove. Rio de Janeiro, RJ, Brasil.
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spelling Honório, Nildimar AlvesCâmara, Daniel Cardoso PortelaWiggins, Keenan WigginsEastmond, BradleyAlto, Barry Wilmer2021-01-07T14:42:08Z2021-01-07T14:42:08Z2020HONÓRIO, Nildimar Alves et al. High-Throughput Method for Detection of Arbovirus Infection of Saliva in Mosquitoes Aedes aegypti and Ae. albopictus. Viruses, v. 12, 1343, 18p, Nov. 2020.1999-4915https://www.arca.fiocruz.br/handle/icict/4552710.3390/v12111343engMDPIMosquito vetoresCompetência vetorialEnsaio de salivaTransmissãoVigilância de arbovírusMosquito vectorsVector competenceSaliva assayTransmissionArbovirus surveillanceHigh-Throughput Method for Detection of Arbovirus Infection of Saliva in Mosquitoes Aedes aegypti and Ae. albopictusinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Mosquitos Transmissores de Hematozoários. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Núcleo Operacional Sentinela de Mosquitos Vetores-Nosmove. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Mosquitos Transmissores de Hematozoários. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Núcleo Operacional Sentinela de Mosquitos Vetores-Nosmove. Rio de Janeiro, RJ, Brasil.IFAS, Department of Entomology and Nematology, Florida Medical Entomology Laboratory. University of Florida. Vero Beach, FL, USA.IFAS, Department of Entomology and Nematology, Florida Medical Entomology Laboratory. University of Florida. Vero Beach, FL, USA.IFAS, Department of Entomology and Nematology, Florida Medical Entomology Laboratory. University of Florida. Vero Beach, FL, USA.Vector competence refers to the ability of a vector to acquire, maintain, and transmit a pathogen. Collecting mosquito saliva in medium-filled capillary tubes has become the standard for approximating arbovirus transmission. However, this method is time-consuming and labor-intensive. Here we compare the capillary tube method to an alternative high-throughput detection method the collection of saliva on paper cards saturated with honey, with (FTA card) and without (filter paper) reagents for the preservation of nucleic acid for Aedes aegypti and Aedes albopictus mosquitoes infected with two emerging genotypes of the chikungunya virus (CHIKV). Model results showed that the Asian genotype CHIKV dissemination in the harvested legs of both Ae. aegypti and Ae. albopictus increased the odds of females having a positive salivary infection and higher salivary viral titers, while for the IOL genotype the same e ect was observed only for Ae. aegypti. Of the three tested detection methods, the FTA card was significantly more e ective at detecting infected saliva of Ae. aegypti and Ae. albopictus females than the capillary tube and filter paper was as e ective as the capillary tube for the Asian genotype. We did not find significant e ects of the detection method in detecting higher viral titer for both Asian and IOL genotypes. Our results are discussed in light of the limitations of the di erent tested detection methods.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/45527/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALNildimarHOnorio_RafaelCamara_etal_IOC_2020.pdfNildimarHOnorio_RafaelCamara_etal_IOC_2020.pdfapplication/pdf1034925https://www.arca.fiocruz.br/bitstream/icict/45527/2/NildimarHOnorio_RafaelCamara_etal_IOC_2020.pdf93446dca8e7214b78260f897d60e2786MD52TEXTNildimarHOnorio_RafaelCamara_etal_IOC_2020.pdf.txtNildimarHOnorio_RafaelCamara_etal_IOC_2020.pdf.txtExtracted texttext/plain65788https://www.arca.fiocruz.br/bitstream/icict/45527/3/NildimarHOnorio_RafaelCamara_etal_IOC_2020.pdf.txtb9402ab75580b9f07af191d4362f7426MD53icict/455272021-01-08 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dc.title.pt_BR.fl_str_mv High-Throughput Method for Detection of Arbovirus Infection of Saliva in Mosquitoes Aedes aegypti and Ae. albopictus
title High-Throughput Method for Detection of Arbovirus Infection of Saliva in Mosquitoes Aedes aegypti and Ae. albopictus
spellingShingle High-Throughput Method for Detection of Arbovirus Infection of Saliva in Mosquitoes Aedes aegypti and Ae. albopictus
Honório, Nildimar Alves
Mosquito vetores
Competência vetorial
Ensaio de saliva
Transmissão
Vigilância de arbovírus
Mosquito vectors
Vector competence
Saliva assay
Transmission
Arbovirus surveillance
title_short High-Throughput Method for Detection of Arbovirus Infection of Saliva in Mosquitoes Aedes aegypti and Ae. albopictus
title_full High-Throughput Method for Detection of Arbovirus Infection of Saliva in Mosquitoes Aedes aegypti and Ae. albopictus
title_fullStr High-Throughput Method for Detection of Arbovirus Infection of Saliva in Mosquitoes Aedes aegypti and Ae. albopictus
title_full_unstemmed High-Throughput Method for Detection of Arbovirus Infection of Saliva in Mosquitoes Aedes aegypti and Ae. albopictus
title_sort High-Throughput Method for Detection of Arbovirus Infection of Saliva in Mosquitoes Aedes aegypti and Ae. albopictus
author Honório, Nildimar Alves
author_facet Honório, Nildimar Alves
Câmara, Daniel Cardoso Portela
Wiggins, Keenan Wiggins
Eastmond, Bradley
Alto, Barry Wilmer
author_role author
author2 Câmara, Daniel Cardoso Portela
Wiggins, Keenan Wiggins
Eastmond, Bradley
Alto, Barry Wilmer
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Honório, Nildimar Alves
Câmara, Daniel Cardoso Portela
Wiggins, Keenan Wiggins
Eastmond, Bradley
Alto, Barry Wilmer
dc.subject.other.pt_BR.fl_str_mv Mosquito vetores
Competência vetorial
Ensaio de saliva
Transmissão
Vigilância de arbovírus
topic Mosquito vetores
Competência vetorial
Ensaio de saliva
Transmissão
Vigilância de arbovírus
Mosquito vectors
Vector competence
Saliva assay
Transmission
Arbovirus surveillance
dc.subject.en.pt_BR.fl_str_mv Mosquito vectors
Vector competence
Saliva assay
Transmission
Arbovirus surveillance
description Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Mosquitos Transmissores de Hematozoários. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Núcleo Operacional Sentinela de Mosquitos Vetores-Nosmove. Rio de Janeiro, RJ, Brasil.
publishDate 2020
dc.date.issued.fl_str_mv 2020
dc.date.accessioned.fl_str_mv 2021-01-07T14:42:08Z
dc.date.available.fl_str_mv 2021-01-07T14:42:08Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv HONÓRIO, Nildimar Alves et al. High-Throughput Method for Detection of Arbovirus Infection of Saliva in Mosquitoes Aedes aegypti and Ae. albopictus. Viruses, v. 12, 1343, 18p, Nov. 2020.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/45527
dc.identifier.issn.pt_BR.fl_str_mv 1999-4915
dc.identifier.doi.none.fl_str_mv 10.3390/v12111343
identifier_str_mv HONÓRIO, Nildimar Alves et al. High-Throughput Method for Detection of Arbovirus Infection of Saliva in Mosquitoes Aedes aegypti and Ae. albopictus. Viruses, v. 12, 1343, 18p, Nov. 2020.
1999-4915
10.3390/v12111343
url https://www.arca.fiocruz.br/handle/icict/45527
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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https://www.arca.fiocruz.br/bitstream/icict/45527/2/NildimarHOnorio_RafaelCamara_etal_IOC_2020.pdf
https://www.arca.fiocruz.br/bitstream/icict/45527/3/NildimarHOnorio_RafaelCamara_etal_IOC_2020.pdf.txt
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