Quantitative proteome and phosphoproteome analyses highlight the adherent population during Trypanosoma cruzi metacyclogenesis
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2017 |
| Outros Autores: | , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | por |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/24935 |
Resumo: | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Genômica Funcional. Curitiba, PR, Brasil. |
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Amorim, Juliana C.Batista, MichelCunha, Elizabeth S. daLucena, Aline Castro RodriguesLima, Carla Vanessa de PaulaSousa, KarlaKrieger, Marco AurélioMarchini, Fabrício Klerynton2018-02-26T13:04:39Z2018-02-26T13:04:39Z2017AMORIM, Juliana C. et al. Quantitative proteome and phosphoproteome analyses highlight the adherent population during Trypanosoma cruzi metacyclogenesis. Scientific Reports, v.9, n.9899, p. 1-12, 2017.2045-2322https://www.arca.fiocruz.br/handle/icict/2493510.1038/s41598-017-10292-3porNature Publishing GroupProteômicaEspectrometria de MassasFosforilaçãoProteomicsMass SpectrometryPhosphorylationProteómicaEspectrometría de MasasFosforilaciónTrypanosoma cruziQuantitative proteome and phosphoproteome analyses highlight the adherent population during Trypanosoma cruzi metacyclogenesisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Genômica Funcional. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Genômica Funcional. Curitiba, PR, Brasil / Fundação Oswaldo Cruz. Instituto Carlos Chagas. Mass Spectrometry Facility - RPT02H. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Genômica Funcional. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Genômica Funcional. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Genômica Funcional. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Genômica Funcional. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Genômica Funcional. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Genômica Funcional. Curitiba, PR, Brasil / Fundação Oswaldo Cruz. Instituto Carlos Chagas. Mass Spectrometry Facility - RPT02H. Curitiba, PR, Brasil.Trypanosoma cruzi metacyclogenesis is a natural process that occurs inside the triatomine vector and corresponds to the differentiation of non-infective epimastigotes into infective metacyclic trypomastigotes. The biochemical alterations necessary for the differentiation process have been widely studied with a focus on adhesion and nutritional stress. Here, using a mass spectrometry approach, a large-scale phospho(proteome) study was performed with the aim of understanding the metacyclogenesis processes in a quantitative manner. The results indicate that major modulations in the phospho(proteome) occur under nutritional stress and after 12 and 24 h of adhesion. Significant changes involve key cellular processes, such as translation, oxidative stress, and the metabolism of macromolecules, including proteins, lipids, and carbohydrates. Analysis of the signalling triggered by kinases and phosphatases from 7,336 identified phosphorylation sites demonstrates that 260 of these sites are modulated throughout the differentiation process, and some of these modulated proteins have previously been identified as drug targets in trypanosomiasis treatment. To the best of our knowledge, this study provides the first quantitative results highlighting the modulation of phosphorylation sites during metacyclogenesis and the greater coverage of the proteome to the parasite during this process. The data are available via ProteomeXchange with identifier number PXD006171.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83084https://www.arca.fiocruz.br/bitstream/icict/24935/1/license.txt783568c2893d2e25a99990b126be1772MD51ORIGINALMarchiniQuantitative.pdfMarchiniQuantitative.pdfapplication/pdf1810106https://www.arca.fiocruz.br/bitstream/icict/24935/2/MarchiniQuantitative.pdfe682582d7564dc33d86684511f9c143dMD52TEXTMarchiniQuantitative.pdf.txtMarchiniQuantitative.pdf.txtExtracted texttext/plain57390https://www.arca.fiocruz.br/bitstream/icict/24935/3/MarchiniQuantitative.pdf.txt58575758bce66f3188192437cbbaf2f7MD53icict/249352019-10-31 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| dc.title.pt_BR.fl_str_mv |
Quantitative proteome and phosphoproteome analyses highlight the adherent population during Trypanosoma cruzi metacyclogenesis |
| title |
Quantitative proteome and phosphoproteome analyses highlight the adherent population during Trypanosoma cruzi metacyclogenesis |
| spellingShingle |
Quantitative proteome and phosphoproteome analyses highlight the adherent population during Trypanosoma cruzi metacyclogenesis Amorim, Juliana C. Proteômica Espectrometria de Massas Fosforilação Proteomics Mass Spectrometry Phosphorylation Proteómica Espectrometría de Masas Fosforilación Trypanosoma cruzi |
| title_short |
Quantitative proteome and phosphoproteome analyses highlight the adherent population during Trypanosoma cruzi metacyclogenesis |
| title_full |
Quantitative proteome and phosphoproteome analyses highlight the adherent population during Trypanosoma cruzi metacyclogenesis |
| title_fullStr |
Quantitative proteome and phosphoproteome analyses highlight the adherent population during Trypanosoma cruzi metacyclogenesis |
| title_full_unstemmed |
Quantitative proteome and phosphoproteome analyses highlight the adherent population during Trypanosoma cruzi metacyclogenesis |
| title_sort |
Quantitative proteome and phosphoproteome analyses highlight the adherent population during Trypanosoma cruzi metacyclogenesis |
| author |
Amorim, Juliana C. |
| author_facet |
Amorim, Juliana C. Batista, Michel Cunha, Elizabeth S. da Lucena, Aline Castro Rodrigues Lima, Carla Vanessa de Paula Sousa, Karla Krieger, Marco Aurélio Marchini, Fabrício Klerynton |
| author_role |
author |
| author2 |
Batista, Michel Cunha, Elizabeth S. da Lucena, Aline Castro Rodrigues Lima, Carla Vanessa de Paula Sousa, Karla Krieger, Marco Aurélio Marchini, Fabrício Klerynton |
| author2_role |
author author author author author author author |
| dc.contributor.author.fl_str_mv |
Amorim, Juliana C. Batista, Michel Cunha, Elizabeth S. da Lucena, Aline Castro Rodrigues Lima, Carla Vanessa de Paula Sousa, Karla Krieger, Marco Aurélio Marchini, Fabrício Klerynton |
| dc.subject.other.pt_BR.fl_str_mv |
Proteômica Espectrometria de Massas Fosforilação |
| topic |
Proteômica Espectrometria de Massas Fosforilação Proteomics Mass Spectrometry Phosphorylation Proteómica Espectrometría de Masas Fosforilación Trypanosoma cruzi |
| dc.subject.en.pt_BR.fl_str_mv |
Proteomics Mass Spectrometry Phosphorylation |
| dc.subject.es.pt_BR.fl_str_mv |
Proteómica Espectrometría de Masas Fosforilación |
| dc.subject.decs.pt_BR.fl_str_mv |
Trypanosoma cruzi |
| description |
Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Genômica Funcional. Curitiba, PR, Brasil. |
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2017 |
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2017 |
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2018-02-26T13:04:39Z |
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2018-02-26T13:04:39Z |
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AMORIM, Juliana C. et al. Quantitative proteome and phosphoproteome analyses highlight the adherent population during Trypanosoma cruzi metacyclogenesis. Scientific Reports, v.9, n.9899, p. 1-12, 2017. |
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https://www.arca.fiocruz.br/handle/icict/24935 |
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2045-2322 |
| dc.identifier.doi.none.fl_str_mv |
10.1038/s41598-017-10292-3 |
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AMORIM, Juliana C. et al. Quantitative proteome and phosphoproteome analyses highlight the adherent population during Trypanosoma cruzi metacyclogenesis. Scientific Reports, v.9, n.9899, p. 1-12, 2017. 2045-2322 10.1038/s41598-017-10292-3 |
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