In Vitro Sensitivity of Paired Leishmania (Viannia) braziliensis Samples Isolated before Meglumine Antimoniate Treatment and after Treatment Failure or Reactivation of Cutaneous Leishmaniasis

Detalhes bibliográficos
Autor(a) principal: Baptista, Cibele
Data de Publicação: 2015
Outros Autores: Miranda, Luciana de Freitas Campos, Madeira, Maria de Fátima, Leon, Leonor Laura Pinto, Silva, Fátima Conceição, Schubach, Armando de Oliveira
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/13638
Resumo: Fundação Oswaldo Cruz. Bio-Manguinhos. Núcleo de Biossegurança. Rio de Janeiro, RJ, Brasil.
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spelling Baptista, CibeleMiranda, Luciana de Freitas CamposMadeira, Maria de FátimaLeon, Leonor Laura PintoSilva, Fátima ConceiçãoSchubach, Armando de Oliveira2016-04-07T13:50:06Z2016-04-07T13:50:06Z2015BAPTISTA, Cibele; et al. In Vitro Sensitivity of Paired Leishmania (Viannia) braziliensis Samples Isolated before Meglumine Antimoniate Treatment and after Treatment Failure or Reactivation of Cutaneous Leishmaniasis. Disease Markers, v.2015, Article ID 943236, 6p, 2015.0278-0240https://www.arca.fiocruz.br/handle/icict/1363810.1155/2015/9432361875-8630engHindawi Publishing CorporationIn Vitro Sensitivity of Paired Leishmania (Viannia) braziliensis Samples Isolated before Meglumine Antimoniate Treatment and after Treatment Failure or Reactivation of Cutaneous Leishmaniasisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Bio-Manguinhos. Núcleo de Biossegurança. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Pesquisa Clínica e Vigilância em Leishmanioses. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Pesquisa Clínica e Vigilância em Leishmanioses. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Laboratório de Bioquímica de Tripanosomatídeos. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunoparasitologia. Rio de Janeiro, RJ, Brasil.This study evaluated the in vitro sensitivity of paired Leishmania braziliensis samples isolated from the same patient before pentavalent antimonial treatment (Sample A) and after treatment failure or cutaneous leishmaniasis reactivation (Sample B) in patients undergoing intralesional administration or injections (5 mgS/kg/d) of meglumine antimoniate. Fourteen samples from 7 patients were studied. After 24 h of drug exposure, 50% lethal dose (LD50) values for promastigotes ranged from 0.37 mg/mL to 5.86 mg/mL for samples obtained before treatment (A) and 0.89 mg/mL to 7.80 mg/mL for samples obtained after treatment (B). After 48 h, LD50 values ranged from 0.37 mg/mL to 5.75 mg/mL and 0.70 mg/mL to 7.68 mg/mL for A and B samples, respectively. After 48 h, LD50 values for amastigotes ranged from 11.7 to 44.3 μg/mL for A samples and 13.7 to 52.7 μg/mL for B samples. Of 7 patients, 1 discontinued treatment and 6 were cured after retreatment with amphotericin B (4 cases) or meglumine antimoniate (2 cases). Overall the B samples had higher LD50 values than A samples; however the difference was not significant. These results do not support the hypothesis that low-dose and intralesional treatments induce selection of resistant parasites in vitro and suggest that other factors may influence therapeutic outcome in patients with poor response to initial treatment.Cutaneous LeishmaniasisIn vitroLeishmania (Viannia) braziliensisLeishmania braziliensisMeglumine Antimoniate TreatmentLeishmaniose cutâneaTécnicas In VitroLeishmania braziliensisMegluminaTratamentoinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; 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dc.title.pt_BR.fl_str_mv In Vitro Sensitivity of Paired Leishmania (Viannia) braziliensis Samples Isolated before Meglumine Antimoniate Treatment and after Treatment Failure or Reactivation of Cutaneous Leishmaniasis
title In Vitro Sensitivity of Paired Leishmania (Viannia) braziliensis Samples Isolated before Meglumine Antimoniate Treatment and after Treatment Failure or Reactivation of Cutaneous Leishmaniasis
spellingShingle In Vitro Sensitivity of Paired Leishmania (Viannia) braziliensis Samples Isolated before Meglumine Antimoniate Treatment and after Treatment Failure or Reactivation of Cutaneous Leishmaniasis
Baptista, Cibele
Cutaneous Leishmaniasis
In vitro
Leishmania (Viannia) braziliensis
Leishmania braziliensis
Meglumine Antimoniate Treatment
Leishmaniose cutânea
Técnicas In Vitro
Leishmania braziliensis
Meglumina
Tratamento
title_short In Vitro Sensitivity of Paired Leishmania (Viannia) braziliensis Samples Isolated before Meglumine Antimoniate Treatment and after Treatment Failure or Reactivation of Cutaneous Leishmaniasis
title_full In Vitro Sensitivity of Paired Leishmania (Viannia) braziliensis Samples Isolated before Meglumine Antimoniate Treatment and after Treatment Failure or Reactivation of Cutaneous Leishmaniasis
title_fullStr In Vitro Sensitivity of Paired Leishmania (Viannia) braziliensis Samples Isolated before Meglumine Antimoniate Treatment and after Treatment Failure or Reactivation of Cutaneous Leishmaniasis
title_full_unstemmed In Vitro Sensitivity of Paired Leishmania (Viannia) braziliensis Samples Isolated before Meglumine Antimoniate Treatment and after Treatment Failure or Reactivation of Cutaneous Leishmaniasis
title_sort In Vitro Sensitivity of Paired Leishmania (Viannia) braziliensis Samples Isolated before Meglumine Antimoniate Treatment and after Treatment Failure or Reactivation of Cutaneous Leishmaniasis
author Baptista, Cibele
author_facet Baptista, Cibele
Miranda, Luciana de Freitas Campos
Madeira, Maria de Fátima
Leon, Leonor Laura Pinto
Silva, Fátima Conceição
Schubach, Armando de Oliveira
author_role author
author2 Miranda, Luciana de Freitas Campos
Madeira, Maria de Fátima
Leon, Leonor Laura Pinto
Silva, Fátima Conceição
Schubach, Armando de Oliveira
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Baptista, Cibele
Miranda, Luciana de Freitas Campos
Madeira, Maria de Fátima
Leon, Leonor Laura Pinto
Silva, Fátima Conceição
Schubach, Armando de Oliveira
dc.subject.en.pt_BR.fl_str_mv Cutaneous Leishmaniasis
In vitro
Leishmania (Viannia) braziliensis
Leishmania braziliensis
Meglumine Antimoniate Treatment
topic Cutaneous Leishmaniasis
In vitro
Leishmania (Viannia) braziliensis
Leishmania braziliensis
Meglumine Antimoniate Treatment
Leishmaniose cutânea
Técnicas In Vitro
Leishmania braziliensis
Meglumina
Tratamento
dc.subject.decs.pt_BR.fl_str_mv Leishmaniose cutânea
Técnicas In Vitro
Leishmania braziliensis
Meglumina
Tratamento
description Fundação Oswaldo Cruz. Bio-Manguinhos. Núcleo de Biossegurança. Rio de Janeiro, RJ, Brasil.
publishDate 2015
dc.date.issued.fl_str_mv 2015
dc.date.accessioned.fl_str_mv 2016-04-07T13:50:06Z
dc.date.available.fl_str_mv 2016-04-07T13:50:06Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv BAPTISTA, Cibele; et al. In Vitro Sensitivity of Paired Leishmania (Viannia) braziliensis Samples Isolated before Meglumine Antimoniate Treatment and after Treatment Failure or Reactivation of Cutaneous Leishmaniasis. Disease Markers, v.2015, Article ID 943236, 6p, 2015.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/13638
dc.identifier.issn.none.fl_str_mv 0278-0240
dc.identifier.doi.none.fl_str_mv 10.1155/2015/943236
dc.identifier.eissn.none.fl_str_mv 1875-8630
identifier_str_mv BAPTISTA, Cibele; et al. In Vitro Sensitivity of Paired Leishmania (Viannia) braziliensis Samples Isolated before Meglumine Antimoniate Treatment and after Treatment Failure or Reactivation of Cutaneous Leishmaniasis. Disease Markers, v.2015, Article ID 943236, 6p, 2015.
0278-0240
10.1155/2015/943236
1875-8630
url https://www.arca.fiocruz.br/handle/icict/13638
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Hindawi Publishing Corporation
publisher.none.fl_str_mv Hindawi Publishing Corporation
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
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collection Repositório Institucional da FIOCRUZ (ARCA)
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