Expression and arrangement of extracellular matrix proteins in the lungs of mice infected with Paracoccidioides brasiliensis conidia

Detalhes bibliográficos
Autor(a) principal: González, Angel
Data de Publicação: 2008
Outros Autores: Lenzi, Henrique Leonel, Mota, Ester Maria, Caputo, Luzia F. G., Restrepo, Angela, Cano, Luz Elena
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/3739
Resumo: The authors thank the personnel of the Departamento de Patologia, Instituto Oswaldo Cruz (FIOCRUZ), Rio de Janeiro, Brazil for their assistance with CLSM and ESM.
id CRUZ_55c7efac99c7dd1f180fa5c75b2ad57b
oai_identifier_str oai:www.arca.fiocruz.br:icict/3739
network_acronym_str CRUZ
network_name_str Repositório Institucional da FIOCRUZ (ARCA)
repository_id_str 2135
spelling González, AngelLenzi, Henrique LeonelMota, Ester MariaCaputo, Luzia F. G.Restrepo, AngelaCano, Luz Elena2012-01-18T13:03:08Z2012-01-18T13:03:08Z2008GONZÁLEZ, A. et al. Expression and arrangement of extracellular matrix proteins in the lungs of mice infected with Paracoccidioides brasiliensis conidia. International Journal of Experimental Pathology. v.89, n.2, p.106-16, Apr. 2008.0959-9673https://www.arca.fiocruz.br/handle/icict/373910.1111/j.1365-2613.2008.00573.xThe authors thank the personnel of the Departamento de Patologia, Instituto Oswaldo Cruz (FIOCRUZ), Rio de Janeiro, Brazil for their assistance with CLSM and ESM.This work was supported by the Instituto Oswaldo Cruz (FIOCRUZ), the Corporación para Investigaciones Biológicas and the Instituto Colombiano para el Desarrollo de la Ciencia y la Tecnología, Francisco José de Caldas, COLCIENCIAS, Bogotá, Colombia; Grant No. 2213-04-191-98. The National Doctoral Program of COLCIENCIAS supported Angel González.Corporación para Investigaciones Biológicas. Medical and Experimental Mycology Group. Medellín, Colômbia / Universidad de Antioquia. Microbiology School. Medellín, Colômbia.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Patologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Patologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Patologia. Rio de Janeiro, RJ, Brasil.Corporación para Investigaciones Biológicas. Medical and Experimental Mycology Group. Medellín, Colômbia.Corporación para Investigaciones Biológicas. Medical and Experimental Mycology Group. Medellín, Colômbia / Universidad de Antioquia. Microbiology School. Medellín, Colômbia.Extracellular matrix (ECM) proteins are important modulators of migration, differentiation and proliferation for the various cell types present in the lungs; they influence the immune response as well as participate in the adherence of several fungi including Paracoccidioides brasiliensis. The expression, deposition and arrangement of ECM proteins such as laminin, fibronectin, fibrinogen, collagen and proteoglycans in the lungs of mice infected with P. brasiliensis conidia has been evaluated in this study, together with the elastic fibre system. Lungs of BALB/c mice infected with P. brasiliensis conidia were analysed for the different ECM proteins by histological and immunohistochemical procedures at different times of infection. In addition, laser scanning confocal microscopy and scanning electron microscopy were used. During the early periods, the lungs of infected animals showed an inflammatory infiltrate composed mainly of polymorphonuclear neutrophils (PMNs) and macrophages, while during the later periods, mice presented a chronic inflammatory response with granuloma formation. Re-arrangement and increased expression of all ECM proteins tested were observed throughout all studied periods, especially during the occurrence of inflammatory infiltration and formation of the granuloma. The elastic fibre system showed an elastolysis process in all experiments. In conclusion, this study provides new details of pulmonary ECM distribution during the course of paracoccidioidomycosis.engWileyExpression and arrangement of extracellular matrix proteins in the lungs of mice infected with Paracoccidioides brasiliensis conidiainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleCollagenExtracellular Matrix ProteinsFibrinogenFibronectinLamininParacoccidioides brasiliensisParacoccidioides brasiliensisColágenoProteínas da Matriz ExtracelularFibrinogênioFibronectinasLamininainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALExpression and arrangement of extracellular matrix proteins.pdfExpression and arrangement of extracellular matrix proteins.pdfapplication/pdf1300178https://www.arca.fiocruz.br/bitstream/icict/3739/1/Expression%20and%20arrangement%20of%20extracellular%20matrix%20proteins.pdfe92b988e19789a0f7761fff5ea6bcb17MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81648https://www.arca.fiocruz.br/bitstream/icict/3739/2/license.txte095249ac7cacefbfe39684dfe45e706MD52TEXTExpression and arrangement of extracellular matrix proteins.pdf.txtExpression and arrangement of extracellular matrix proteins.pdf.txtExtracted texttext/plain44302https://www.arca.fiocruz.br/bitstream/icict/3739/5/Expression%20and%20arrangement%20of%20extracellular%20matrix%20proteins.pdf.txt82218a743c00d8e1c339896f9ad731a8MD55THUMBNAILExpression and arrangement of extracellular matrix proteins.pdf.jpgExpression and arrangement of extracellular matrix proteins.pdf.jpgGenerated Thumbnailimage/jpeg2219https://www.arca.fiocruz.br/bitstream/icict/3739/4/Expression%20and%20arrangement%20of%20extracellular%20matrix%20proteins.pdf.jpg4a771876ad1de9b90d16863bb2b4bb12MD54icict/37392018-04-06 09:06:39.278oai:www.arca.fiocruz.br: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Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352018-04-06T12:06:39Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Expression and arrangement of extracellular matrix proteins in the lungs of mice infected with Paracoccidioides brasiliensis conidia
title Expression and arrangement of extracellular matrix proteins in the lungs of mice infected with Paracoccidioides brasiliensis conidia
spellingShingle Expression and arrangement of extracellular matrix proteins in the lungs of mice infected with Paracoccidioides brasiliensis conidia
González, Angel
Collagen
Extracellular Matrix Proteins
Fibrinogen
Fibronectin
Laminin
Paracoccidioides brasiliensis
Paracoccidioides brasiliensis
Colágeno
Proteínas da Matriz Extracelular
Fibrinogênio
Fibronectinas
Laminina
title_short Expression and arrangement of extracellular matrix proteins in the lungs of mice infected with Paracoccidioides brasiliensis conidia
title_full Expression and arrangement of extracellular matrix proteins in the lungs of mice infected with Paracoccidioides brasiliensis conidia
title_fullStr Expression and arrangement of extracellular matrix proteins in the lungs of mice infected with Paracoccidioides brasiliensis conidia
title_full_unstemmed Expression and arrangement of extracellular matrix proteins in the lungs of mice infected with Paracoccidioides brasiliensis conidia
title_sort Expression and arrangement of extracellular matrix proteins in the lungs of mice infected with Paracoccidioides brasiliensis conidia
author González, Angel
author_facet González, Angel
Lenzi, Henrique Leonel
Mota, Ester Maria
Caputo, Luzia F. G.
Restrepo, Angela
Cano, Luz Elena
author_role author
author2 Lenzi, Henrique Leonel
Mota, Ester Maria
Caputo, Luzia F. G.
Restrepo, Angela
Cano, Luz Elena
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv González, Angel
Lenzi, Henrique Leonel
Mota, Ester Maria
Caputo, Luzia F. G.
Restrepo, Angela
Cano, Luz Elena
dc.subject.en.pt_BR.fl_str_mv Collagen
Extracellular Matrix Proteins
Fibrinogen
Fibronectin
Laminin
Paracoccidioides brasiliensis
topic Collagen
Extracellular Matrix Proteins
Fibrinogen
Fibronectin
Laminin
Paracoccidioides brasiliensis
Paracoccidioides brasiliensis
Colágeno
Proteínas da Matriz Extracelular
Fibrinogênio
Fibronectinas
Laminina
dc.subject.decs.pt_BR.fl_str_mv Paracoccidioides brasiliensis
Colágeno
Proteínas da Matriz Extracelular
Fibrinogênio
Fibronectinas
Laminina
description The authors thank the personnel of the Departamento de Patologia, Instituto Oswaldo Cruz (FIOCRUZ), Rio de Janeiro, Brazil for their assistance with CLSM and ESM.
publishDate 2008
dc.date.issued.fl_str_mv 2008
dc.date.accessioned.fl_str_mv 2012-01-18T13:03:08Z
dc.date.available.fl_str_mv 2012-01-18T13:03:08Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv GONZÁLEZ, A. et al. Expression and arrangement of extracellular matrix proteins in the lungs of mice infected with Paracoccidioides brasiliensis conidia. International Journal of Experimental Pathology. v.89, n.2, p.106-16, Apr. 2008.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/3739
dc.identifier.issn.none.fl_str_mv 0959-9673
dc.identifier.doi.none.fl_str_mv 10.1111/j.1365-2613.2008.00573.x
identifier_str_mv GONZÁLEZ, A. et al. Expression and arrangement of extracellular matrix proteins in the lungs of mice infected with Paracoccidioides brasiliensis conidia. International Journal of Experimental Pathology. v.89, n.2, p.106-16, Apr. 2008.
0959-9673
10.1111/j.1365-2613.2008.00573.x
url https://www.arca.fiocruz.br/handle/icict/3739
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/3739/1/Expression%20and%20arrangement%20of%20extracellular%20matrix%20proteins.pdf
https://www.arca.fiocruz.br/bitstream/icict/3739/2/license.txt
https://www.arca.fiocruz.br/bitstream/icict/3739/5/Expression%20and%20arrangement%20of%20extracellular%20matrix%20proteins.pdf.txt
https://www.arca.fiocruz.br/bitstream/icict/3739/4/Expression%20and%20arrangement%20of%20extracellular%20matrix%20proteins.pdf.jpg
bitstream.checksum.fl_str_mv e92b988e19789a0f7761fff5ea6bcb17
e095249ac7cacefbfe39684dfe45e706
82218a743c00d8e1c339896f9ad731a8
4a771876ad1de9b90d16863bb2b4bb12
bitstream.checksumAlgorithm.fl_str_mv MD5
MD5
MD5
MD5
repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
repository.mail.fl_str_mv repositorio.arca@fiocruz.br
_version_ 1813009102711816192

Registros relacionados