Peculiarities of Trypanosoma rangeli KP1(–) Strains Isolated from the Wild Rodent Phyllomys dasythrix (Santa Catarina, Brazil): Comparisons with T. rangeli KP1(+) strains and Trypanosoma lewisi (Kinetoplastea, Trypanosomatidae)

Detalhes bibliográficos
Autor(a) principal: Sousa, Maria A.
Data de Publicação: 2017
Outros Autores: Santos, Barbara N., Carvalhal, Cristina, Steindel, Mario
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/24952
Resumo: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Toxoplasmose e outras Protozooses. Rio de Janeiro, RJ. Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Coleção de Tripanossomatídeos. Rio de Janeiro, RJ, Brasil.
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spelling Sousa, Maria A.Santos, Barbara N.Carvalhal, CristinaSteindel, Mario2018-02-27T11:08:02Z2018-02-27T11:08:02Z2017SOUSA, Maria A. et al. Peculiarities of Trypanosoma rangeli KP1(–) Strains Isolated from the Wild Rodent Phyllomys dasythrix (Santa Catarina, Brazil): Comparisons with T. rangeli KP1(+) strains and Trypanosoma lewisi (Kinetoplastea, Trypanosomatidae). Acta Protozool., v. 56, p.119–127, 2017.0065-1583https://www.arca.fiocruz.br/handle/icict/2495210.4467/16890027AP.17.010.7485engPolish Academy of SciencesmorfologiaTrypanosoma lewisibiologiaReação em Cadeia da PolimeraseisoenzimaKP1(+) and KP1(–) Trypanosoma rangeli strainsTrypanosoma lewisimorphologybiologyisoenzymesPCR productsPeculiarities of Trypanosoma rangeli KP1(–) Strains Isolated from the Wild Rodent Phyllomys dasythrix (Santa Catarina, Brazil): Comparisons with T. rangeli KP1(+) strains and Trypanosoma lewisi (Kinetoplastea, Trypanosomatidae)info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Toxoplasmose e outras Protozooses. Rio de Janeiro, RJ. Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Coleção de Tripanossomatídeos. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Toxoplasmose e outras Protozooses. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Toxoplasmose e outras Protozooses. Rio de Janeiro, RJ. Brasil.Universidade Federal de Santa Catarina. Departamento de Microbiologia, Imunologia e Parasitologia. Laboratórios de Protozoologia. Florianópolis, SC, Brasil.Two KP1(–) strains of Trypanosoma rangeli (SC-58, SC-61) isolated from the wild rodent Phyllomys dasythrix from Santa Catarina (Brazil) were compared with some KP1(+) reference stocks from different Latin America countries, and also with Trypanosoma lewisi. The strains were analyzed by some morphological and biological features, and by biochemical and molecular techniques. The mean total length (TL) of the bloodstream trypomastigotes of T. rangeli varied between 31.3–33.0 μm, and those of T. lewisi (adult forms) was 28.2 μm, values within the variation known for each species. In T. rangeli KP1(+) and T. lewisi, the nucleus was located in the anterior portion of the body, with nuclear indexes (NI) ≥ 1.2, as typically described for both species. Differently, most trypomastigotes of the KP1(–) stocks presented NI ≤ 1.0. Another striking feature of the KP1(–) strains was their very fastidiously growth in axenic cultures when compared with the KP1(+) stocks and T. lewisi. Three isoenzyme loci (MDH, IDH and PGM) clearly distinguished T. rangeli and T. lewisi, and the distinction between the KP1(+) and KP1(–) strains was possible at MDH, PGM and GPI loci. All T. rangeli strains presented the typical 760 bp amplicon derived from their KP2 minicircles. However, the KP3 products of the KP1(+) strains were a single large band (~330bp), whereas those of the KP1(–) had two distinct bands (350 and 300 bp). T. lewisi presented 700 and 400 bp amplicons, as previously reported. The peculiarities of T. rangeli isolates from P. dasythrix corroborate a possible speciation process within this taxon.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/24952/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALbarbara_santos_etal_IOC_2017.pdfbarbara_santos_etal_IOC_2017.pdfapplication/pdf749038https://www.arca.fiocruz.br/bitstream/icict/24952/2/barbara_santos_etal_IOC_2017.pdfe48673a39418d7428c7f61264a7a9ffbMD52TEXTbarbara_santos_etal_IOC_2017.pdf.txtbarbara_santos_etal_IOC_2017.pdf.txtExtracted texttext/plain37039https://www.arca.fiocruz.br/bitstream/icict/24952/3/barbara_santos_etal_IOC_2017.pdf.txt1b41bf956fbba6ac7a62279a951e88a3MD53icict/249522018-08-15 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dc.title.pt_BR.fl_str_mv Peculiarities of Trypanosoma rangeli KP1(–) Strains Isolated from the Wild Rodent Phyllomys dasythrix (Santa Catarina, Brazil): Comparisons with T. rangeli KP1(+) strains and Trypanosoma lewisi (Kinetoplastea, Trypanosomatidae)
title Peculiarities of Trypanosoma rangeli KP1(–) Strains Isolated from the Wild Rodent Phyllomys dasythrix (Santa Catarina, Brazil): Comparisons with T. rangeli KP1(+) strains and Trypanosoma lewisi (Kinetoplastea, Trypanosomatidae)
spellingShingle Peculiarities of Trypanosoma rangeli KP1(–) Strains Isolated from the Wild Rodent Phyllomys dasythrix (Santa Catarina, Brazil): Comparisons with T. rangeli KP1(+) strains and Trypanosoma lewisi (Kinetoplastea, Trypanosomatidae)
Sousa, Maria A.
morfologia
Trypanosoma lewisi
biologia
Reação em Cadeia da Polimerase
isoenzima
KP1(+) and KP1(–) Trypanosoma rangeli strains
Trypanosoma lewisi
morphology
biology
isoenzymes
PCR products
title_short Peculiarities of Trypanosoma rangeli KP1(–) Strains Isolated from the Wild Rodent Phyllomys dasythrix (Santa Catarina, Brazil): Comparisons with T. rangeli KP1(+) strains and Trypanosoma lewisi (Kinetoplastea, Trypanosomatidae)
title_full Peculiarities of Trypanosoma rangeli KP1(–) Strains Isolated from the Wild Rodent Phyllomys dasythrix (Santa Catarina, Brazil): Comparisons with T. rangeli KP1(+) strains and Trypanosoma lewisi (Kinetoplastea, Trypanosomatidae)
title_fullStr Peculiarities of Trypanosoma rangeli KP1(–) Strains Isolated from the Wild Rodent Phyllomys dasythrix (Santa Catarina, Brazil): Comparisons with T. rangeli KP1(+) strains and Trypanosoma lewisi (Kinetoplastea, Trypanosomatidae)
title_full_unstemmed Peculiarities of Trypanosoma rangeli KP1(–) Strains Isolated from the Wild Rodent Phyllomys dasythrix (Santa Catarina, Brazil): Comparisons with T. rangeli KP1(+) strains and Trypanosoma lewisi (Kinetoplastea, Trypanosomatidae)
title_sort Peculiarities of Trypanosoma rangeli KP1(–) Strains Isolated from the Wild Rodent Phyllomys dasythrix (Santa Catarina, Brazil): Comparisons with T. rangeli KP1(+) strains and Trypanosoma lewisi (Kinetoplastea, Trypanosomatidae)
author Sousa, Maria A.
author_facet Sousa, Maria A.
Santos, Barbara N.
Carvalhal, Cristina
Steindel, Mario
author_role author
author2 Santos, Barbara N.
Carvalhal, Cristina
Steindel, Mario
author2_role author
author
author
dc.contributor.author.fl_str_mv Sousa, Maria A.
Santos, Barbara N.
Carvalhal, Cristina
Steindel, Mario
dc.subject.other.pt_BR.fl_str_mv morfologia
Trypanosoma lewisi
biologia
Reação em Cadeia da Polimerase
isoenzima
topic morfologia
Trypanosoma lewisi
biologia
Reação em Cadeia da Polimerase
isoenzima
KP1(+) and KP1(–) Trypanosoma rangeli strains
Trypanosoma lewisi
morphology
biology
isoenzymes
PCR products
dc.subject.en.pt_BR.fl_str_mv KP1(+) and KP1(–) Trypanosoma rangeli strains
Trypanosoma lewisi
morphology
biology
isoenzymes
PCR products
description Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Toxoplasmose e outras Protozooses. Rio de Janeiro, RJ. Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Coleção de Tripanossomatídeos. Rio de Janeiro, RJ, Brasil.
publishDate 2017
dc.date.issued.fl_str_mv 2017
dc.date.accessioned.fl_str_mv 2018-02-27T11:08:02Z
dc.date.available.fl_str_mv 2018-02-27T11:08:02Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv SOUSA, Maria A. et al. Peculiarities of Trypanosoma rangeli KP1(–) Strains Isolated from the Wild Rodent Phyllomys dasythrix (Santa Catarina, Brazil): Comparisons with T. rangeli KP1(+) strains and Trypanosoma lewisi (Kinetoplastea, Trypanosomatidae). Acta Protozool., v. 56, p.119–127, 2017.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/24952
dc.identifier.issn.pt_BR.fl_str_mv 0065-1583
dc.identifier.doi.none.fl_str_mv 10.4467/16890027AP.17.010.7485
identifier_str_mv SOUSA, Maria A. et al. Peculiarities of Trypanosoma rangeli KP1(–) Strains Isolated from the Wild Rodent Phyllomys dasythrix (Santa Catarina, Brazil): Comparisons with T. rangeli KP1(+) strains and Trypanosoma lewisi (Kinetoplastea, Trypanosomatidae). Acta Protozool., v. 56, p.119–127, 2017.
0065-1583
10.4467/16890027AP.17.010.7485
url https://www.arca.fiocruz.br/handle/icict/24952
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Polish Academy of Sciences
publisher.none.fl_str_mv Polish Academy of Sciences
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
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