Genetic diversity of Leishmania infantum field populations from Brazil
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2012 |
| Outros Autores: | , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | por |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/7861 |
Resumo: | Universidade Federal do Espírito Santo. Núcleo de Doenças Infecciosas. Laboratório de Leishmanioses. Vitória, ES, Brasil/Universidade Federal de Minas Gerais. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brasil |
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Segatto, MarcelaRibeiro, Lucas SecchimCosta, Dorcas LamounierCosta, Carlos Henrique NeryOliveira, Márcia Rosa deCarvalho, Sílvio Fernando Guimarães deDietze, ReynaldoBrito, Cristiana Ferreira Alves deLemos, Elenice Moreira2014-07-02T14:34:43Z2014-07-02T14:34:43Z2012SEGATTO, Marcela et al. Genetic diversity of Leishmania infantum field populations from Brazil. Memórias do Instituto Oswaldo Cruz. 2012, vol.107, pp. 39-47.0074-0276https://www.arca.fiocruz.br/handle/icict/786110.1590/S0074-02762012000100006porFundação Oswaldo CruzGenetic diversity of Leishmania infantum field populations from Brazilinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal do Espírito Santo. Núcleo de Doenças Infecciosas. Laboratório de Leishmanioses. Vitória, ES, Brasil/Universidade Federal de Minas Gerais. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, BrasilUniversidade Federal do Espírito Santo. Núcleo de Doenças Infecciosas. Laboratório de Leishmanioses. Vitória, ES, Brasil/Universidade Federal de Minas Gerais. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, BrasilInstituto Natan Portella para Doenças Tropicais. Teresina, PI, BrasilInstituto Natan Portella para Doenças Tropicais. Teresina, PI, BrasilUniversidade Federal da Paraíba. Departamento de Biologia Celular. João Pessoa, PB, BrasilUniversidade Estadual de Montes Claros. Montes Claros, MG, BrasilUniversidade Federal do Espírito Santo. Núcleo de Doenças Infecciosas. Laboratório de Leishmanioses. Vitória, ES, BrasilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, BrasilUniversidade Federal do Espírito Santo. Núcleo de Doenças Infecciosas. Laboratório de Leishmanioses. Vitória, ES, BrasilLeishmania infantum (syn. Leishmania chagasi) is the etiological agent of visceral leishmaniasis (VL) in Brazil. The epidemiology of VL is poorly understood. Therefore, a more detailed molecular characterization at an intraspecific level is certainly needed. Herein, three independent molecular methods, multilocus microsatellite typing (MLMT), random amplification of polymorphic DNA (RAPD) and simple sequence repeats-polymerase chain reaction (SSR-PCR), were used to evaluate the genetic diversity of 53 L. infantum isolates from five different endemic areas in Brazil. Population structures were inferred by distance-based and Bayesian-based approaches. Eighteen very similar genotypes were detected by MLMT, most of them differed in only one locus and no correlation was found between MLMT profiles, geographical origin or the estimated population structure. However, complex profiles composed of 182 bands obtained by both RAPD and SSR-PCR assays gave different results. Unweighted pair group method with arithmetic mean trees built from these data revealed a high degree of homogeneity within isolates of L. infantum. Interestingly, despite this genetic homogeneity, most of the isolates clustered according to their geographical origin.Leishmania infantumgenetic diversityMLMTRAPDSSR-PCRinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALGenetic diversity of Leishmania infantum field populations from Brazil..pdfGenetic diversity of Leishmania infantum field populations from Brazil..pdfapplication/pdf517223https://www.arca.fiocruz.br/bitstream/icict/7861/1/Genetic%20diversity%20of%20Leishmania%20infantum%20field%20populations%20from%20Brazil..pdf1b6cb0fd0cd2aaf956be916ff4d47779MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://www.arca.fiocruz.br/bitstream/icict/7861/2/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD52TEXTGenetic diversity of Leishmania infantum field populations from Brazil..pdf.txtGenetic diversity of Leishmania infantum field populations from Brazil..pdf.txtExtracted texttext/plain40365https://www.arca.fiocruz.br/bitstream/icict/7861/3/Genetic%20diversity%20of%20Leishmania%20infantum%20field%20populations%20from%20Brazil..pdf.txt5e9f089003c75dc53ae1bf23b953bac1MD53icict/78612018-04-06 11:25:53.793oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352018-04-06T14:25:53Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false |
| dc.title.pt_BR.fl_str_mv |
Genetic diversity of Leishmania infantum field populations from Brazil |
| title |
Genetic diversity of Leishmania infantum field populations from Brazil |
| spellingShingle |
Genetic diversity of Leishmania infantum field populations from Brazil Segatto, Marcela Leishmania infantum genetic diversity MLMT RAPD SSR-PCR |
| title_short |
Genetic diversity of Leishmania infantum field populations from Brazil |
| title_full |
Genetic diversity of Leishmania infantum field populations from Brazil |
| title_fullStr |
Genetic diversity of Leishmania infantum field populations from Brazil |
| title_full_unstemmed |
Genetic diversity of Leishmania infantum field populations from Brazil |
| title_sort |
Genetic diversity of Leishmania infantum field populations from Brazil |
| author |
Segatto, Marcela |
| author_facet |
Segatto, Marcela Ribeiro, Lucas Secchim Costa, Dorcas Lamounier Costa, Carlos Henrique Nery Oliveira, Márcia Rosa de Carvalho, Sílvio Fernando Guimarães de Dietze, Reynaldo Brito, Cristiana Ferreira Alves de Lemos, Elenice Moreira |
| author_role |
author |
| author2 |
Ribeiro, Lucas Secchim Costa, Dorcas Lamounier Costa, Carlos Henrique Nery Oliveira, Márcia Rosa de Carvalho, Sílvio Fernando Guimarães de Dietze, Reynaldo Brito, Cristiana Ferreira Alves de Lemos, Elenice Moreira |
| author2_role |
author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Segatto, Marcela Ribeiro, Lucas Secchim Costa, Dorcas Lamounier Costa, Carlos Henrique Nery Oliveira, Márcia Rosa de Carvalho, Sílvio Fernando Guimarães de Dietze, Reynaldo Brito, Cristiana Ferreira Alves de Lemos, Elenice Moreira |
| dc.subject.en.pt_BR.fl_str_mv |
Leishmania infantum genetic diversity MLMT RAPD SSR-PCR |
| topic |
Leishmania infantum genetic diversity MLMT RAPD SSR-PCR |
| description |
Universidade Federal do Espírito Santo. Núcleo de Doenças Infecciosas. Laboratório de Leishmanioses. Vitória, ES, Brasil/Universidade Federal de Minas Gerais. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brasil |
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2012 |
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2012 |
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2014-07-02T14:34:43Z |
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2014-07-02T14:34:43Z |
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SEGATTO, Marcela et al. Genetic diversity of Leishmania infantum field populations from Brazil. Memórias do Instituto Oswaldo Cruz. 2012, vol.107, pp. 39-47. |
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https://www.arca.fiocruz.br/handle/icict/7861 |
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0074-0276 |
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10.1590/S0074-02762012000100006 |
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SEGATTO, Marcela et al. Genetic diversity of Leishmania infantum field populations from Brazil. Memórias do Instituto Oswaldo Cruz. 2012, vol.107, pp. 39-47. 0074-0276 10.1590/S0074-02762012000100006 |
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