Canine visceral leishmaniasis: study of methods for the detection of IgG in serum and eluate samples

Detalhes bibliográficos
Autor(a) principal: Figueiredo, Fabiano B.
Data de Publicação: 2010
Outros Autores: Madeira, Maria F., Nascimento, Lílian D., Abrantes, Tuanne Rotti, Mouta-Confort, Eliame, Passos, Sonia Regina L., Schubach, Tânia Maria P.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/32200
Resumo: Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Laboratório de Pesquisa Clínica em Dermatozoonoses em Animais Domésticos. Rio de Janeiro, RJ, Brasil.
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spelling Figueiredo, Fabiano B.Madeira, Maria F.Nascimento, Lílian D.Abrantes, Tuanne RottiMouta-Confort, EliamePassos, Sonia Regina L.Schubach, Tânia Maria P.2019-03-21T20:40:49Z2019-03-21T20:40:49Z2010FIGUEIREDO, Fabiano B. et al. Canine visceral leishmaniasis: study of methods for the detection of IgG inserum and eluate samples. Revista do Instituto de Medicina Tropical de São Paulo, v. 52, n.4, p. 193-196, July/Aug. 2010.0036-4665https://www.arca.fiocruz.br/handle/icict/3220010.1590/S0036-466520100004000051678-9946engUniversidade de São PauloCanine visceral leishmaniasis: study of methods for the detection of IgG in serum and eluate samplesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Laboratório de Pesquisa Clínica em Dermatozoonoses em Animais Domésticos. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Laboratório de Vigilância em Leishmanioses. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Laboratório de Vigilância em Leishmanioses. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Laboratório de Pesquisa Clínica em Dermatozoonoses em Animais Domésticos. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Laboratório de Vigilância em Leishmanioses. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas Laboratório de Epidemiologia Clínica. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Laboratório de Pesquisa Clínica em Dermatozoonoses em Animais Domésticos. Rio de Janeiro, RJ, Brasil.The Brazilian Ministry of Health recommends the culling and euthanasia of dogs with a positive serological test for canine visceral leishmaniasis (CVL). In the Municipality of Rio de Janeiro, the technique used for the diagnosis of CVL is the indirect fluorescent antibody test (IFAT), using blood samples eluted on filter paper (eluate). A dog survey was conducted over a period of one year in the region of Carapiá, in order to evaluate the diagnosis of CVL in this region. All animals underwent clinical examination, and blood samples (serum and eluate) were collected for analysis by enzyme immunoassay (ELISA) and IFAT. A skin biopsy was obtained for parasitological examination (culture). A total of 305 animals were studied and Leishmania chagasi was isolated from nine animals. Sensitivity and specificity were 100% and 96.6% for ELISA, respectively, 100% and 65.5% for IFAT (cut-off at a 1:40 dilution), 100% and 83.4% for IFAT (cut-off at a 1:80 dilution), and 22.2% and 97.0% for eluate IFAT. In conclusion, ELISA was the best tool for the diagnosis of CVL among the serological techniques tested. The present results suggest the need for a better evaluation of filter paper IFAT as the only diagnostic method for CVL in the Municipality of Rio de Janeiro.LeishmaniaDogDiagnosisSerological methodsRio de Janeiroinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/32200/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALve_Figueiredo_Fabiano_etal_INI_2010.pdfve_Figueiredo_Fabiano_etal_INI_2010.pdfapplication/pdf119028https://www.arca.fiocruz.br/bitstream/icict/32200/2/ve_Figueiredo_Fabiano_etal_INI_2010.pdf5ba888b15ae7693b87b1af82ed9135cfMD52TEXTve_Figueiredo_Fabiano_etal_INI_2010.pdf.txtve_Figueiredo_Fabiano_etal_INI_2010.pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv Canine visceral leishmaniasis: study of methods for the detection of IgG in serum and eluate samples
title Canine visceral leishmaniasis: study of methods for the detection of IgG in serum and eluate samples
spellingShingle Canine visceral leishmaniasis: study of methods for the detection of IgG in serum and eluate samples
Figueiredo, Fabiano B.
Leishmania
Dog
Diagnosis
Serological methods
Rio de Janeiro
title_short Canine visceral leishmaniasis: study of methods for the detection of IgG in serum and eluate samples
title_full Canine visceral leishmaniasis: study of methods for the detection of IgG in serum and eluate samples
title_fullStr Canine visceral leishmaniasis: study of methods for the detection of IgG in serum and eluate samples
title_full_unstemmed Canine visceral leishmaniasis: study of methods for the detection of IgG in serum and eluate samples
title_sort Canine visceral leishmaniasis: study of methods for the detection of IgG in serum and eluate samples
author Figueiredo, Fabiano B.
author_facet Figueiredo, Fabiano B.
Madeira, Maria F.
Nascimento, Lílian D.
Abrantes, Tuanne Rotti
Mouta-Confort, Eliame
Passos, Sonia Regina L.
Schubach, Tânia Maria P.
author_role author
author2 Madeira, Maria F.
Nascimento, Lílian D.
Abrantes, Tuanne Rotti
Mouta-Confort, Eliame
Passos, Sonia Regina L.
Schubach, Tânia Maria P.
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Figueiredo, Fabiano B.
Madeira, Maria F.
Nascimento, Lílian D.
Abrantes, Tuanne Rotti
Mouta-Confort, Eliame
Passos, Sonia Regina L.
Schubach, Tânia Maria P.
dc.subject.en.pt_BR.fl_str_mv Leishmania
Dog
Diagnosis
Serological methods
Rio de Janeiro
topic Leishmania
Dog
Diagnosis
Serological methods
Rio de Janeiro
description Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Laboratório de Pesquisa Clínica em Dermatozoonoses em Animais Domésticos. Rio de Janeiro, RJ, Brasil.
publishDate 2010
dc.date.issued.fl_str_mv 2010
dc.date.accessioned.fl_str_mv 2019-03-21T20:40:49Z
dc.date.available.fl_str_mv 2019-03-21T20:40:49Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv FIGUEIREDO, Fabiano B. et al. Canine visceral leishmaniasis: study of methods for the detection of IgG inserum and eluate samples. Revista do Instituto de Medicina Tropical de São Paulo, v. 52, n.4, p. 193-196, July/Aug. 2010.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/32200
dc.identifier.issn.pt_BR.fl_str_mv 0036-4665
dc.identifier.doi.none.fl_str_mv 10.1590/S0036-46652010000400005
dc.identifier.eissn.none.fl_str_mv 1678-9946
identifier_str_mv FIGUEIREDO, Fabiano B. et al. Canine visceral leishmaniasis: study of methods for the detection of IgG inserum and eluate samples. Revista do Instituto de Medicina Tropical de São Paulo, v. 52, n.4, p. 193-196, July/Aug. 2010.
0036-4665
10.1590/S0036-46652010000400005
1678-9946
url https://www.arca.fiocruz.br/handle/icict/32200
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Universidade de São Paulo
publisher.none.fl_str_mv Universidade de São Paulo
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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