Distribution and Genetic Diversity of Bacteriocin Gene Clusters in Rumen Microbial Genomes.

Detalhes bibliográficos
Autor(a) principal: Azevedo, Analice Claudia
Data de Publicação: 2015
Outros Autores: Bento, Cláudia Braga Pereira, Ruiz, Jeronimo Conceição, Queiroz, Marisa Vieira de, Mantovani, Hilário Cuquetto
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/15729
Resumo: Universidade Federal de Viçosa. Departamento de Microbiologia. Viçosa, MG, Brasil
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spelling Azevedo, Analice ClaudiaBento, Cláudia Braga PereiraRuiz, Jeronimo ConceiçãoQueiroz, Marisa Vieira deMantovani, Hilário Cuquetto2016-09-08T17:19:55Z2016-09-08T17:19:55Z2015AZEVEDO, Analice Claudia et al. Distribution and Genetic Diversity of Bacteriocin Gene Clusters in Rumen Microbial Genomes. Appl Environ Microbiol., vol. 81, n. 20, p. 7290-7304.0099-2240https://www.arca.fiocruz.br/handle/icict/1572910.1128/AEM.01223-15engAmerican Society for MicrobiologyDistribution and Genetic Diversity of Bacteriocin Gene Clusters in Rumen Microbial Genomes.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal de Viçosa. Departamento de Microbiologia. Viçosa, MG, BrasilFundação Oswaldo Cruz. Centro de Pesquisa René Rachou. Belo Horizonte, MG, BrasilFundação Oswaldo Cruz. Centro de Pesquisa René Rachou. Belo Horizonte, MG, BrasilUniversidade Federal de Viçosa. Departamento de Microbiologia. Viçosa, MG, BrasilUniversidade Federal de Viçosa. Departamento de Microbiologia. Viçosa, MG, BrasilSome species of ruminal bacteria are known to produce antimicrobial peptides, but the screening procedures have mostly been based on in vitro assays using standardized methods. Recent sequencing efforts have made available the genome sequences of hundreds of ruminal microorganisms. In this work, we performed genome mining of the complete and partial genome sequences of 224 ruminal bacteria and 5 ruminal archaea to determine the distribution and diversity of bacteriocin gene clusters. A total of 46 bacteriocin gene clusters were identified in 33 strains of ruminal bacteria. Twenty gene clusters were related to lanthipeptide biosynthesis, while 11 gene clusters were associated with sactipeptide production, 7 gene clusters were associated with class II bacteriocin production, and 8 gene clusters were associated with class III bacteriocin production. The frequency of strains whose genomes encode putative antimicrobial peptide precursors was 14.4%. Clusters related to the production of sactipeptides were identified for the first time among ruminal bacteria. BLAST analysis indicated that the majority of the gene clusters (88%) encoding putative lanthipeptides contained all the essential genes required for lanthipeptide biosynthesis. Most strains of Streptococcus (66.6%) harbored complete lanthipeptide gene clusters, in addition to an open reading frame encoding a putative class II bacteriocin. Albusin B-like proteins were found in 100% of the Ruminococcus albus strains screened in this study. The in silico analysis provided evidence of novel biosynthetic gene clusters in bacterial species not previously related to bacteriocin production, suggesting that the rumen microbiota represents an underexplored source of antimicrobial peptides.ruminal microorganismsinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/15729/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALve_Analice_Azevedo_Distribuition_CPqRR_2015.pdfve_Analice_Azevedo_Distribuition_CPqRR_2015.pdfapplication/pdf9690769https://www.arca.fiocruz.br/bitstream/icict/15729/2/ve_Analice_Azevedo_Distribuition_CPqRR_2015.pdfb1f5ff8cde8041fa182c2e6cb693a3c2MD52TEXTve_Analice_Azevedo_Distribuition_CPqRR_2015.pdf.txtve_Analice_Azevedo_Distribuition_CPqRR_2015.pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv Distribution and Genetic Diversity of Bacteriocin Gene Clusters in Rumen Microbial Genomes.
title Distribution and Genetic Diversity of Bacteriocin Gene Clusters in Rumen Microbial Genomes.
spellingShingle Distribution and Genetic Diversity of Bacteriocin Gene Clusters in Rumen Microbial Genomes.
Azevedo, Analice Claudia
ruminal microorganisms
title_short Distribution and Genetic Diversity of Bacteriocin Gene Clusters in Rumen Microbial Genomes.
title_full Distribution and Genetic Diversity of Bacteriocin Gene Clusters in Rumen Microbial Genomes.
title_fullStr Distribution and Genetic Diversity of Bacteriocin Gene Clusters in Rumen Microbial Genomes.
title_full_unstemmed Distribution and Genetic Diversity of Bacteriocin Gene Clusters in Rumen Microbial Genomes.
title_sort Distribution and Genetic Diversity of Bacteriocin Gene Clusters in Rumen Microbial Genomes.
author Azevedo, Analice Claudia
author_facet Azevedo, Analice Claudia
Bento, Cláudia Braga Pereira
Ruiz, Jeronimo Conceição
Queiroz, Marisa Vieira de
Mantovani, Hilário Cuquetto
author_role author
author2 Bento, Cláudia Braga Pereira
Ruiz, Jeronimo Conceição
Queiroz, Marisa Vieira de
Mantovani, Hilário Cuquetto
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Azevedo, Analice Claudia
Bento, Cláudia Braga Pereira
Ruiz, Jeronimo Conceição
Queiroz, Marisa Vieira de
Mantovani, Hilário Cuquetto
dc.subject.en.pt_BR.fl_str_mv ruminal microorganisms
topic ruminal microorganisms
description Universidade Federal de Viçosa. Departamento de Microbiologia. Viçosa, MG, Brasil
publishDate 2015
dc.date.issued.fl_str_mv 2015
dc.date.accessioned.fl_str_mv 2016-09-08T17:19:55Z
dc.date.available.fl_str_mv 2016-09-08T17:19:55Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv AZEVEDO, Analice Claudia et al. Distribution and Genetic Diversity of Bacteriocin Gene Clusters in Rumen Microbial Genomes. Appl Environ Microbiol., vol. 81, n. 20, p. 7290-7304.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/15729
dc.identifier.issn.pt_BR.fl_str_mv 0099-2240
dc.identifier.doi.none.fl_str_mv 10.1128/AEM.01223-15
identifier_str_mv AZEVEDO, Analice Claudia et al. Distribution and Genetic Diversity of Bacteriocin Gene Clusters in Rumen Microbial Genomes. Appl Environ Microbiol., vol. 81, n. 20, p. 7290-7304.
0099-2240
10.1128/AEM.01223-15
url https://www.arca.fiocruz.br/handle/icict/15729
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv American Society for Microbiology
publisher.none.fl_str_mv American Society for Microbiology
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
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collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/15729/1/license.txt
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