Revisiting the Trypanosoma cruzi metacyclogenesis: morphological and ultrastructural analyses during cell differentiation

Detalhes bibliográficos
Autor(a) principal: Gonçalves, Camila Silva
Data de Publicação: 2018
Outros Autores: Ávila, Andréa Rodrigues, Souza, Wanderley de, Motta, Maria Cristina Machado, Cavalcanti, Danielle Pereira
Tipo de documento: Artigo
Idioma: por
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/30018
Resumo: Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Ultraestrutura Celular Hertha Meyer. Rio de Janeiro, RJ, Brasil / Instituto Nacional de Metrologia, Qualidade e Tecnologia. Diretoria de Metrologia Aplicada às Ciências da Vida. Laboratório de Microbiologia. Rio de Janeiro, RJ, Brasil.
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spelling Gonçalves, Camila SilvaÁvila, Andréa RodriguesSouza, Wanderley deMotta, Maria Cristina MachadoCavalcanti, Danielle Pereira2018-11-14T11:16:23Z2018-11-14T11:16:23Z2018GONÇALVES, Camila Silva et al. Revisiting the Trypanosoma cruzi metacyclogenesis: morphological and ultrastructural analyses during cell differentiation. Parasites & Vectors, v. 11, n. 83, p. 1-14, 2018.1756-3305https://www.arca.fiocruz.br/handle/icict/3001810.1186/s13071-018-2664-41756-3305porBMCRevisiting the Trypanosoma cruzi metacyclogenesis: morphological and ultrastructural analyses during cell differentiationinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Ultraestrutura Celular Hertha Meyer. Rio de Janeiro, RJ, Brasil / Instituto Nacional de Metrologia, Qualidade e Tecnologia. Diretoria de Metrologia Aplicada às Ciências da Vida. Laboratório de Microbiologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Regulação da Expressão Gênica. Curitiba, PR, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Ultraestrutura Celular Hertha Meyer. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Instituto Nacional de Ciência e Tecnologia de Biologia Estrutural e Bioimagem. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Ultraestrutura Celular Hertha Meyer. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Instituto Nacional de Ciência e Tecnologia de Biologia Estrutural e Bioimagem. Rio de Janeiro, RJ, Brasil.Instituto Nacional de Metrologia, Qualidade e Tecnologia. Diretoria de Metrologia Aplicada às Ciências da Vida. Laboratório de Microbiologia. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Instituto Nacional de Ciência e Tecnologia de Biologia Estrutural e Bioimagem. Rio de Janeiro, RJ, Brasil.Trypanosoma cruzi uses several strategies to survive in different hosts. A key step in the life-cycle of this parasite is metacyclogenesis, which involves various morphological, biochemical, and genetic changes that induce the differentiation of non-pathogenic epimastigotes into pathogenic metacyclic trypomastigotes. During metacyclogenesis, T. cruzi displays distinct morphologies and ultrastructural features, which have not been fully characterized. We performed a temporal description of metacyclogenesis using different microscopy techniques that resulted in the identification of three intermediate forms of T. cruzi: intermediates I, II and III. Such classification was based on morphological and ultrastructural aspects as the location of the kinetoplast in relation to the nucleus, kinetoplast shape and kDNA topology. Furthermore, we suggested that metacyclic trypomastigotes derived from intermediate forms that had already detached from the substrate. We also found that changes in the kinetoplast morphology and kDNA arrangement occurred only after the repositioning of this structure toward the posterior region of the cell body. These changes occurred during the later stages of differentiation. In contrast, changes in the nucleus shape began as soon as metacyclogenesis was initiated, while changes in nuclear ultrastructure, such as the loss of the nucleolus, were only observed during later stages of differentiation. Finally, we found that kDNA networks of distinct T. cruzi forms present different patterns of DNA topology. This way, the study of T. cruzi metacyclogenesis revealed important aspects of the morphology and ultrastructure of this intriguing cell differentiation process. This research expands our understanding of this parasite’s fascinating life-cycle. It also highlights the study of T. cruzi as an important and exciting model system for investigating diverse aspects of cellular, molecular, and evolutionary biology.Cell DifferentiationDNA, KinetoplastMicroscopyUltrastructureDiferenciación CelularADN de CinetoplastoMicroscopíaUltraestructuraDiferenciação CelularDNA de CinetoplastoMicroscopiaTrypanosoma cruziUltraestruturainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83084https://www.arca.fiocruz.br/bitstream/icict/30018/1/license.txt783568c2893d2e25a99990b126be1772MD51ORIGINALParasitVectors_Article_26.pdfParasitVectors_Article_26.pdfapplication/pdf5986593https://www.arca.fiocruz.br/bitstream/icict/30018/2/ParasitVectors_Article_26.pdfcf41e4332569b4f3b2d5f37410ba8004MD52TEXTParasitVectors_Article_26.pdf.txtParasitVectors_Article_26.pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv Revisiting the Trypanosoma cruzi metacyclogenesis: morphological and ultrastructural analyses during cell differentiation
title Revisiting the Trypanosoma cruzi metacyclogenesis: morphological and ultrastructural analyses during cell differentiation
spellingShingle Revisiting the Trypanosoma cruzi metacyclogenesis: morphological and ultrastructural analyses during cell differentiation
Gonçalves, Camila Silva
Cell Differentiation
DNA, Kinetoplast
Microscopy
Ultrastructure
Diferenciación Celular
ADN de Cinetoplasto
Microscopía
Ultraestructura
Diferenciação Celular
DNA de Cinetoplasto
Microscopia
Trypanosoma cruzi
Ultraestrutura
title_short Revisiting the Trypanosoma cruzi metacyclogenesis: morphological and ultrastructural analyses during cell differentiation
title_full Revisiting the Trypanosoma cruzi metacyclogenesis: morphological and ultrastructural analyses during cell differentiation
title_fullStr Revisiting the Trypanosoma cruzi metacyclogenesis: morphological and ultrastructural analyses during cell differentiation
title_full_unstemmed Revisiting the Trypanosoma cruzi metacyclogenesis: morphological and ultrastructural analyses during cell differentiation
title_sort Revisiting the Trypanosoma cruzi metacyclogenesis: morphological and ultrastructural analyses during cell differentiation
author Gonçalves, Camila Silva
author_facet Gonçalves, Camila Silva
Ávila, Andréa Rodrigues
Souza, Wanderley de
Motta, Maria Cristina Machado
Cavalcanti, Danielle Pereira
author_role author
author2 Ávila, Andréa Rodrigues
Souza, Wanderley de
Motta, Maria Cristina Machado
Cavalcanti, Danielle Pereira
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Gonçalves, Camila Silva
Ávila, Andréa Rodrigues
Souza, Wanderley de
Motta, Maria Cristina Machado
Cavalcanti, Danielle Pereira
dc.subject.en.pt_BR.fl_str_mv Cell Differentiation
DNA, Kinetoplast
topic Cell Differentiation
DNA, Kinetoplast
Microscopy
Ultrastructure
Diferenciación Celular
ADN de Cinetoplasto
Microscopía
Ultraestructura
Diferenciação Celular
DNA de Cinetoplasto
Microscopia
Trypanosoma cruzi
Ultraestrutura
dc.subject.en.En.fl_str_mv Microscopy
Ultrastructure
dc.subject.es.pt_BR.fl_str_mv Diferenciación Celular
ADN de Cinetoplasto
dc.subject.es.Es.fl_str_mv Microscopía
Ultraestructura
dc.subject.decs.pt_BR.fl_str_mv Diferenciação Celular
DNA de Cinetoplasto
Microscopia
Trypanosoma cruzi
Ultraestrutura
description Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Ultraestrutura Celular Hertha Meyer. Rio de Janeiro, RJ, Brasil / Instituto Nacional de Metrologia, Qualidade e Tecnologia. Diretoria de Metrologia Aplicada às Ciências da Vida. Laboratório de Microbiologia. Rio de Janeiro, RJ, Brasil.
publishDate 2018
dc.date.accessioned.fl_str_mv 2018-11-14T11:16:23Z
dc.date.available.fl_str_mv 2018-11-14T11:16:23Z
dc.date.issued.fl_str_mv 2018
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv GONÇALVES, Camila Silva et al. Revisiting the Trypanosoma cruzi metacyclogenesis: morphological and ultrastructural analyses during cell differentiation. Parasites & Vectors, v. 11, n. 83, p. 1-14, 2018.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/30018
dc.identifier.issn.pt_BR.fl_str_mv 1756-3305
dc.identifier.doi.none.fl_str_mv 10.1186/s13071-018-2664-4
dc.identifier.eissn.none.fl_str_mv 1756-3305
identifier_str_mv GONÇALVES, Camila Silva et al. Revisiting the Trypanosoma cruzi metacyclogenesis: morphological and ultrastructural analyses during cell differentiation. Parasites & Vectors, v. 11, n. 83, p. 1-14, 2018.
1756-3305
10.1186/s13071-018-2664-4
url https://www.arca.fiocruz.br/handle/icict/30018
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv BMC
publisher.none.fl_str_mv BMC
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
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instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
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