Predominance of Giardia duodenalis AII sub-assemblage in young children from Salvador, Bahia, Brazil
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2020 |
| Outros Autores: | , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/42800 |
Resumo: | Fundação de Amparo à Pesquisa do Estado da Bahia (FAPESB), Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq/MCT) and Universidade Federal da Bahia (UFBA), Brazil. |
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Pacheco, Flávia Thamiris FigueiredoSilva, Renata Kelly Novaes RodriguesCarvalho, Silvia Souza deRocha, Felipe CarvalhoChagas, Gisele Maria Trindade dasGomes, Daisy ChagasRibeiro Junior, Hugo da CostaRibeiro, Tereza Cristina MedradoMattos, Ângela Peixoto deSilva, Luciano KalabricSoares, Neci MatosTeixeira, Márcia Cristina Aquino2020-08-17T17:04:40Z2020-08-17T17:04:40Z2020PACHECO, Flávia Thamiris Figueiredo et al. Predominance of Giardia duodenalis AII sub-assemblage in young children from Salvador, Bahia, Brazil. Biomédica, v. 40, n. 3, 2020.,0120-4157https://www.arca.fiocruz.br/handle/icict/42800Fundação de Amparo à Pesquisa do Estado da Bahia (FAPESB), Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq/MCT) and Universidade Federal da Bahia (UFBA), Brazil.Objetivo. Caracterizar los aislamientos moleculares de Giardia duodenalis en niños de Salvador, Bahia, Brasil. Materiales y métodos. Las muestras fecales positivas para G. duodenalis se obtuvieron de 71 niños de dos guarderías y de 39 usuarios de un laboratorio público de análisis clínicos. Las muestras fueram analizadas por PCR-RFLP de los genes de glutamato deshidrogenasa (gdh) y β-giardin, y por secuenciación de β-giardin. Resultados. De las 110 muestras de G. duodenalis, 80 (72,7%) amplificaron uno o ambos genes analizados. De estos, 62 (77,5%) se identificaron como el ensamblaje A y 18 (22,5%) como el ensamblaje B. El subconjunto AII se identificó en el 58,8% (n=47) de los aislamientos, seguido del subconjunto AI (18,8%, n=15), BIV (11,2%, n=9) y BIII (5,0%, n=4). El subconjunto de AII fue el más frecuente en los niños de ambas guarderías, mientras que la AI solo se encontró en el grupo atendido en el laboratorio clínico. Hubo un predominio de sub-ensamblaje AII en niños menores de dos años. Conclusiones. La mayor frecuencia de subconjuntos AII sugiere que la transmisión antroponótica es más común en Salvador, pero que también existen vías de transmisión zoonóticas, y que un cambio en la susceptibilidad a diferentes patrones moleculares de Giardia puede ocurrir durante el crecimiento infantil.Universidade Federal da Bahia. Faculdade de Farmácia. Salvador, BA, Brasil.Universidade Federal da Bahia. Faculdade de Farmácia. Salvador, BA, Brasil.Universidade Federal da Bahia. Faculdade de Farmácia. Salvador, BA, Brasil.Universidade Federal da Bahia. Faculdade de Farmácia. Salvador, BA, Brasil.Universidade Federal da Bahia. Faculdade de Farmácia. Salvador, BA, Brasil.Universidade Federal da Bahia. Faculdade de Farmácia. Salvador, BA, Brasil.Universidade Federal da Bahia. Centro Pediátrico Professor Hosannah de Oliveira. Salvador, BA, Brasil.Universidade Federal da Bahia. Centro Pediátrico Professor Hosannah de Oliveira. Salvador, BA, Brasil.Universidade Federal da Bahia. Centro Pediátrico Professor Hosannah de Oliveira. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil.Universidade Federal da Bahia. Faculdade de Farmácia. Salvador, BA, Brasil.Objective. To characterize the Giardia duodenalis molecular isolates in children from Salvador, Bahia, Brazil. Methods. G. duodenalis positive fecal samples were obtained from 71 children from two daycare centers and from 39 users of a public clinical analysis laboratory. Samples were analyzed by PCR-RFLP of the glutamate dehydrogenase (gdh) and β-giardin genes, and by sequencing of β-giardin. Results. Of the 110 G. duodenalis samples, 80 (72.7%) amplified one or both target genes analyzed. Of these, 62 (77.5 %) were identified as assemblage A, and 18 (22.5%) as assemblage B. The sub-assemblage AII was identified in 58.8% (n = 47) of isolates, followed by sub-assemblage AI (18.8%, n = 15), BIV (11.2%, n = 9) and BIII (5.0%, n = 4). The AII sub-assemblage was the most frequent in children of both day care centers, whereas AI was found only in the group attended at the clinical laboratory. There was a predominance of sub-assemblage AII in children under two years. Conclusions. The higher frequency of AII sub-assemblage suggests that anthroponotic transmission is more common in Salvador, but that zoonotic transmission pathways are also present, and that a change in susceptibility to different molecular patterns of Giardia may occur during child growth.engInstituto Nacional de SaludGiardíase / epidemiologiaCriançaBrasilGiardiasis / epidemiologiaNiñoGuarderíasBrasil.Predominance of Giardia duodenalis AII sub-assemblage in young children from Salvador, Bahia, BrazilPredominance of Giardia duodenalis AII sub-assemblage in young children from Salvador, Bahia, Brazil Predominio del subconjunto AII de Giardia duodenalis en niños pequeños de Salvador, Bahía, Brasilinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/42800/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALPacheco, Flávia Thamiris Predominance of giardia....pdfPacheco, Flávia Thamiris Predominance of giardia....pdfapplication/pdf392373https://www.arca.fiocruz.br/bitstream/icict/42800/2/Pacheco%2c%20Fl%c3%a1via%20Thamiris%20%20Predominance%20of%20giardia....pdf27f9a6349d689cead68a6e4527bd2a3aMD52TEXTPacheco, Flávia Thamiris Predominance of giardia....pdf.txtPacheco, Flávia Thamiris Predominance of giardia....pdf.txtExtracted texttext/plain46993https://www.arca.fiocruz.br/bitstream/icict/42800/3/Pacheco%2c%20Fl%c3%a1via%20Thamiris%20%20Predominance%20of%20giardia....pdf.txt461904f80bbb34ee215ede685891a044MD53icict/428002023-03-15 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| dc.title.pt_BR.fl_str_mv |
Predominance of Giardia duodenalis AII sub-assemblage in young children from Salvador, Bahia, Brazil |
| dc.title.alternative.pt_BR.fl_str_mv |
Predominance of Giardia duodenalis AII sub-assemblage in young children from Salvador, Bahia, Brazil Predominio del subconjunto AII de Giardia duodenalis en niños pequeños de Salvador, Bahía, Brasil |
| title |
Predominance of Giardia duodenalis AII sub-assemblage in young children from Salvador, Bahia, Brazil |
| spellingShingle |
Predominance of Giardia duodenalis AII sub-assemblage in young children from Salvador, Bahia, Brazil Pacheco, Flávia Thamiris Figueiredo Giardíase / epidemiologia Criança Brasil Giardiasis / epidemiologia Niño Guarderías Brasil. |
| title_short |
Predominance of Giardia duodenalis AII sub-assemblage in young children from Salvador, Bahia, Brazil |
| title_full |
Predominance of Giardia duodenalis AII sub-assemblage in young children from Salvador, Bahia, Brazil |
| title_fullStr |
Predominance of Giardia duodenalis AII sub-assemblage in young children from Salvador, Bahia, Brazil |
| title_full_unstemmed |
Predominance of Giardia duodenalis AII sub-assemblage in young children from Salvador, Bahia, Brazil |
| title_sort |
Predominance of Giardia duodenalis AII sub-assemblage in young children from Salvador, Bahia, Brazil |
| author |
Pacheco, Flávia Thamiris Figueiredo |
| author_facet |
Pacheco, Flávia Thamiris Figueiredo Silva, Renata Kelly Novaes Rodrigues Carvalho, Silvia Souza de Rocha, Felipe Carvalho Chagas, Gisele Maria Trindade das Gomes, Daisy Chagas Ribeiro Junior, Hugo da Costa Ribeiro, Tereza Cristina Medrado Mattos, Ângela Peixoto de Silva, Luciano Kalabric Soares, Neci Matos Teixeira, Márcia Cristina Aquino |
| author_role |
author |
| author2 |
Silva, Renata Kelly Novaes Rodrigues Carvalho, Silvia Souza de Rocha, Felipe Carvalho Chagas, Gisele Maria Trindade das Gomes, Daisy Chagas Ribeiro Junior, Hugo da Costa Ribeiro, Tereza Cristina Medrado Mattos, Ângela Peixoto de Silva, Luciano Kalabric Soares, Neci Matos Teixeira, Márcia Cristina Aquino |
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author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Pacheco, Flávia Thamiris Figueiredo Silva, Renata Kelly Novaes Rodrigues Carvalho, Silvia Souza de Rocha, Felipe Carvalho Chagas, Gisele Maria Trindade das Gomes, Daisy Chagas Ribeiro Junior, Hugo da Costa Ribeiro, Tereza Cristina Medrado Mattos, Ângela Peixoto de Silva, Luciano Kalabric Soares, Neci Matos Teixeira, Márcia Cristina Aquino |
| dc.subject.other.pt_BR.fl_str_mv |
Giardíase / epidemiologia Criança Brasil |
| topic |
Giardíase / epidemiologia Criança Brasil Giardiasis / epidemiologia Niño Guarderías Brasil. |
| dc.subject.en.pt_BR.fl_str_mv |
Giardiasis / epidemiologia Niño Guarderías Brasil. |
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Fundação de Amparo à Pesquisa do Estado da Bahia (FAPESB), Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq/MCT) and Universidade Federal da Bahia (UFBA), Brazil. |
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2020 |
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2020-08-17T17:04:40Z |
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2020-08-17T17:04:40Z |
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2020 |
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PACHECO, Flávia Thamiris Figueiredo et al. Predominance of Giardia duodenalis AII sub-assemblage in young children from Salvador, Bahia, Brazil. Biomédica, v. 40, n. 3, 2020., |
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https://www.arca.fiocruz.br/handle/icict/42800 |
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0120-4157 |
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PACHECO, Flávia Thamiris Figueiredo et al. Predominance of Giardia duodenalis AII sub-assemblage in young children from Salvador, Bahia, Brazil. Biomédica, v. 40, n. 3, 2020., 0120-4157 |
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Instituto Nacional de Salud |
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Instituto Nacional de Salud |
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