The stepwise selection for ketoconazole resistance induces upregulation of C14-demethylase (CYP51) in Leishmania amazonensis

Detalhes bibliográficos
Autor(a) principal: Andrade Neto, Valter Viana
Data de Publicação: 2012
Outros Autores: Guedes, Herbert Leonel de Matos, Gomes, Daniel Cláudio de Oliveira, Cavalheiro, Marilene Marcuzzo do Canto, Bergmann, Bartira Rossi, Santos, Eduardo Caio Torres
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/19059
Resumo: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Bioquímica de Tripanosomatídeos. Rio de Janeiro, RJ. Brasil.
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spelling Andrade Neto, Valter VianaGuedes, Herbert Leonel de MatosGomes, Daniel Cláudio de OliveiraCavalheiro, Marilene Marcuzzo do CantoBergmann, Bartira RossiSantos, Eduardo Caio Torres2017-05-30T13:23:16Z2017-05-30T13:23:16Z2012ANDRADE NETO, Valter Viana; et al. The stepwise selection for ketoconazole resistance induces upregulation of C14-demethylase (CYP51) in Leishmania amazonensis. Mem Inst Oswaldo Cruz, v..107, n.3, p.416-419. May 2012.0074-0276https://www.arca.fiocruz.br/handle/icict/190591678-8060engFundação Oswaldo Cruz. Instituto Oswaldo CruzResistência a medicamentosAzóisErgosterolBiossínteseLeishmaniadrug resistanceazolesergosterol biosynthesisLeishmania amazonensisThe stepwise selection for ketoconazole resistance induces upregulation of C14-demethylase (CYP51) in Leishmania amazonensisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Bioquímica de Tripanosomatídeos. Rio de Janeiro, RJ. Brasil.Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil / Universidade Federal do Espírito Santo. Departamento de Patologia. Núcleo de Doenças Infecciosas. Vitória, ES, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Bioquímica de Tripanosomatídeos. Rio de Janeiro, RJ. Brasil.Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Bioquímica de Tripanosomatídeos. Rio de Janeiro, RJ. Brasil.Ketoconazole is a clinically safe antifungal agent that also inhibits the growth of Leishmania spp. A study was undertaken to determine whether Leishmania parasites are prone to becoming resistant to ketoconazole by upregulating C14-demethylase after stepwise pharmacological pressure. Leishmania amazonensis promastigotes [inhibitory concentration (IC)50 = 2 μM] were subjected to stepwise selection with ketoconazole and two resistant lines were obtained, La8 (IC50 = 8 μM) and La10 (IC50 = 10 μM). As a result, we found that the resistance level was directly proportional to the C14-demethylase mRNA expression level; we also observed that expression levels were six and 12 times higher in La8 and La10, respectively. This is the first demonstration that L. amazonensis can up-regulate C14-demethylase in response to drug pressure and this report contributes to the understanding of the mechanisms of parasite resistance.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/19059/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALvalter_andradeneto_etal_IOC_2012.pdfvalter_andradeneto_etal_IOC_2012.pdfapplication/pdf347278https://www.arca.fiocruz.br/bitstream/icict/19059/2/valter_andradeneto_etal_IOC_2012.pdf9b4ad924b1b0e8f9e0f27bd308ee03a8MD52TEXTvalter_andradeneto_etal_IOC_2012.pdf.txtvalter_andradeneto_etal_IOC_2012.pdf.txtExtracted texttext/plain19086https://www.arca.fiocruz.br/bitstream/icict/19059/3/valter_andradeneto_etal_IOC_2012.pdf.txt7a3871c6adead808164e01b4739172b3MD53icict/190592018-08-15 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dc.title.pt_BR.fl_str_mv The stepwise selection for ketoconazole resistance induces upregulation of C14-demethylase (CYP51) in Leishmania amazonensis
title The stepwise selection for ketoconazole resistance induces upregulation of C14-demethylase (CYP51) in Leishmania amazonensis
spellingShingle The stepwise selection for ketoconazole resistance induces upregulation of C14-demethylase (CYP51) in Leishmania amazonensis
Andrade Neto, Valter Viana
Resistência a medicamentos
Azóis
Ergosterol
Biossíntese
Leishmania
drug resistance
azoles
ergosterol biosynthesis
Leishmania amazonensis
title_short The stepwise selection for ketoconazole resistance induces upregulation of C14-demethylase (CYP51) in Leishmania amazonensis
title_full The stepwise selection for ketoconazole resistance induces upregulation of C14-demethylase (CYP51) in Leishmania amazonensis
title_fullStr The stepwise selection for ketoconazole resistance induces upregulation of C14-demethylase (CYP51) in Leishmania amazonensis
title_full_unstemmed The stepwise selection for ketoconazole resistance induces upregulation of C14-demethylase (CYP51) in Leishmania amazonensis
title_sort The stepwise selection for ketoconazole resistance induces upregulation of C14-demethylase (CYP51) in Leishmania amazonensis
author Andrade Neto, Valter Viana
author_facet Andrade Neto, Valter Viana
Guedes, Herbert Leonel de Matos
Gomes, Daniel Cláudio de Oliveira
Cavalheiro, Marilene Marcuzzo do Canto
Bergmann, Bartira Rossi
Santos, Eduardo Caio Torres
author_role author
author2 Guedes, Herbert Leonel de Matos
Gomes, Daniel Cláudio de Oliveira
Cavalheiro, Marilene Marcuzzo do Canto
Bergmann, Bartira Rossi
Santos, Eduardo Caio Torres
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Andrade Neto, Valter Viana
Guedes, Herbert Leonel de Matos
Gomes, Daniel Cláudio de Oliveira
Cavalheiro, Marilene Marcuzzo do Canto
Bergmann, Bartira Rossi
Santos, Eduardo Caio Torres
dc.subject.other.pt_BR.fl_str_mv Resistência a medicamentos
Azóis
Ergosterol
Biossíntese
Leishmania
topic Resistência a medicamentos
Azóis
Ergosterol
Biossíntese
Leishmania
drug resistance
azoles
ergosterol biosynthesis
Leishmania amazonensis
dc.subject.en.pt_BR.fl_str_mv drug resistance
azoles
ergosterol biosynthesis
Leishmania amazonensis
description Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Bioquímica de Tripanosomatídeos. Rio de Janeiro, RJ. Brasil.
publishDate 2012
dc.date.issued.fl_str_mv 2012
dc.date.accessioned.fl_str_mv 2017-05-30T13:23:16Z
dc.date.available.fl_str_mv 2017-05-30T13:23:16Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv ANDRADE NETO, Valter Viana; et al. The stepwise selection for ketoconazole resistance induces upregulation of C14-demethylase (CYP51) in Leishmania amazonensis. Mem Inst Oswaldo Cruz, v..107, n.3, p.416-419. May 2012.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/19059
dc.identifier.issn.pt_BR.fl_str_mv 0074-0276
dc.identifier.eissn.none.fl_str_mv 1678-8060
identifier_str_mv ANDRADE NETO, Valter Viana; et al. The stepwise selection for ketoconazole resistance induces upregulation of C14-demethylase (CYP51) in Leishmania amazonensis. Mem Inst Oswaldo Cruz, v..107, n.3, p.416-419. May 2012.
0074-0276
1678-8060
url https://www.arca.fiocruz.br/handle/icict/19059
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Fundação Oswaldo Cruz. Instituto Oswaldo Cruz
publisher.none.fl_str_mv Fundação Oswaldo Cruz. Instituto Oswaldo Cruz
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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https://www.arca.fiocruz.br/bitstream/icict/19059/2/valter_andradeneto_etal_IOC_2012.pdf
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