Rotavirus A in Brazil: Molecular Epidemiology and Surveillance during 2018-2019

Detalhes bibliográficos
Autor(a) principal: Gutierrez, Meylin Bautista
Data de Publicação: 2020
Outros Autores: Fialho, Alexandre Madi, Maranhão, Adriana Gonçalves, Malta, Fábio Correia, Andrade, Juliana da Silva Ribeiro de, Assis, Rosane Maria Santos de, Mouta, Sérgio da Silva E., Miagostovich, Marize Pereira, Leite, José Paulo Gagliardi, Fumian, Tulio Machado
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/42622
Resumo: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ, Brasil.
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spelling Gutierrez, Meylin BautistaFialho, Alexandre MadiMaranhão, Adriana GonçalvesMalta, Fábio CorreiaAndrade, Juliana da Silva Ribeiro deAssis, Rosane Maria Santos deMouta, Sérgio da Silva E.Miagostovich, Marize PereiraLeite, José Paulo GagliardiFumian, Tulio Machado2020-08-10T19:01:30Z2020-08-10T19:01:30Z2020GUTIERREZ, Meylin Bautista et al. Rotavirus A in Brazil: Molecular Epidemiology and Surveillance during 2018–2019. Pathogens, v. 9, n. 515, p. 1-15, 2020.2076-0817https://www.arca.fiocruz.br/handle/icict/4262210.3390/pathogens9070515engMDPIGastroenterite agudaRotavirus AGenotipagemIncidênciaBrasilAcute gastroenteritisRotavirus AIncidenceGenotypingBrazilRotavirus A in Brazil: Molecular Epidemiology and Surveillance during 2018-2019info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ, Brasil.Rotavirus A (RVA) vaccines succeeded in lowering the burden of acute gastroenteritis (AGE) worldwide, especially preventing severe disease and mortality. In 2019, Brazil completed 13 years of RVA vaccine implementation (Rotarix™) within the National Immunization Program (NIP), and as reported elsewhere, the use of Rotarix™ in the country has reduced childhood mortality and morbidity due to AGE. Even though both marketed vaccines are widely distributed, the surveillance of RVA causing AGE and the monitoring of circulating genotypes are important tools to keep tracking the epidemiological scenario and vaccines impact. Thus, our study investigated RVA epidemiological features, viral load and G and P genotypes circulation in children and adults presenting AGE symptoms in eleven states from three out of five regions in Brazil. By using TaqMan®-based one-step RT-qPCR, we investigated a total of 1536 stool samples collected from symptomatic inpatients, emergency department visits and outpatients from January 2018 to December 2019. G and P genotypes of RVA-positive samples were genetically characterized by multiplex RT-PCR or by nearly complete fragment sequencing. We detected RVA in 12% of samples, 10.5% in 2018 and 13.7% in 2019. A marked winter/spring seasonality was observed, especially in Southern Brazil. The most affected age group was children aged >24-60 months, with a positivity rate of 18.8% (p < 0.05). Evaluating shedding, we found a statistically lower RVA viral load in stool samples collected from children aged up to six months compared to the other age groups (p < 0.05). The genotype G3P[8] was the most prevalent during the two years (83.7% in 2018 and 65.5% in 2019), and nucleotide sequencing of some strains demonstrated that they belonged to the emergent equine-like G3P[8] genotype. The dominance of an emergent genotype causing AGE reinforces the need for continuous epidemiological surveillance to assess the impact of mass RVA immunization as well as to monitor the emergence of novel genotypes.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/42622/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALTulioMFumian_JosePGLeite_etal_IOC_2020.pdfTulioMFumian_JosePGLeite_etal_IOC_2020.pdfapplication/pdf2367681https://www.arca.fiocruz.br/bitstream/icict/42622/2/TulioMFumian_JosePGLeite_etal_IOC_2020.pdff2762355e9d1db7456d6a2ea5a6015eaMD52TEXTTulioMFumian_JosePGLeite_etal_IOC_2020.pdf.txtTulioMFumian_JosePGLeite_etal_IOC_2020.pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv Rotavirus A in Brazil: Molecular Epidemiology and Surveillance during 2018-2019
title Rotavirus A in Brazil: Molecular Epidemiology and Surveillance during 2018-2019
spellingShingle Rotavirus A in Brazil: Molecular Epidemiology and Surveillance during 2018-2019
Gutierrez, Meylin Bautista
Gastroenterite aguda
Rotavirus A
Genotipagem
Incidência
Brasil
Acute gastroenteritis
Rotavirus A
Incidence
Genotyping
Brazil
title_short Rotavirus A in Brazil: Molecular Epidemiology and Surveillance during 2018-2019
title_full Rotavirus A in Brazil: Molecular Epidemiology and Surveillance during 2018-2019
title_fullStr Rotavirus A in Brazil: Molecular Epidemiology and Surveillance during 2018-2019
title_full_unstemmed Rotavirus A in Brazil: Molecular Epidemiology and Surveillance during 2018-2019
title_sort Rotavirus A in Brazil: Molecular Epidemiology and Surveillance during 2018-2019
author Gutierrez, Meylin Bautista
author_facet Gutierrez, Meylin Bautista
Fialho, Alexandre Madi
Maranhão, Adriana Gonçalves
Malta, Fábio Correia
Andrade, Juliana da Silva Ribeiro de
Assis, Rosane Maria Santos de
Mouta, Sérgio da Silva E.
Miagostovich, Marize Pereira
Leite, José Paulo Gagliardi
Fumian, Tulio Machado
author_role author
author2 Fialho, Alexandre Madi
Maranhão, Adriana Gonçalves
Malta, Fábio Correia
Andrade, Juliana da Silva Ribeiro de
Assis, Rosane Maria Santos de
Mouta, Sérgio da Silva E.
Miagostovich, Marize Pereira
Leite, José Paulo Gagliardi
Fumian, Tulio Machado
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Gutierrez, Meylin Bautista
Fialho, Alexandre Madi
Maranhão, Adriana Gonçalves
Malta, Fábio Correia
Andrade, Juliana da Silva Ribeiro de
Assis, Rosane Maria Santos de
Mouta, Sérgio da Silva E.
Miagostovich, Marize Pereira
Leite, José Paulo Gagliardi
Fumian, Tulio Machado
dc.subject.other.pt_BR.fl_str_mv Gastroenterite aguda
Rotavirus A
Genotipagem
Incidência
Brasil
topic Gastroenterite aguda
Rotavirus A
Genotipagem
Incidência
Brasil
Acute gastroenteritis
Rotavirus A
Incidence
Genotyping
Brazil
dc.subject.en.pt_BR.fl_str_mv Acute gastroenteritis
Rotavirus A
Incidence
Genotyping
Brazil
description Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ, Brasil.
publishDate 2020
dc.date.accessioned.fl_str_mv 2020-08-10T19:01:30Z
dc.date.available.fl_str_mv 2020-08-10T19:01:30Z
dc.date.issued.fl_str_mv 2020
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv GUTIERREZ, Meylin Bautista et al. Rotavirus A in Brazil: Molecular Epidemiology and Surveillance during 2018–2019. Pathogens, v. 9, n. 515, p. 1-15, 2020.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/42622
dc.identifier.issn.pt_BR.fl_str_mv 2076-0817
dc.identifier.doi.none.fl_str_mv 10.3390/pathogens9070515
identifier_str_mv GUTIERREZ, Meylin Bautista et al. Rotavirus A in Brazil: Molecular Epidemiology and Surveillance during 2018–2019. Pathogens, v. 9, n. 515, p. 1-15, 2020.
2076-0817
10.3390/pathogens9070515
url https://www.arca.fiocruz.br/handle/icict/42622
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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