Norovirus Recombinant Strains Isolated from Gastroenteritis Outbreaks in Southern Brazil, 2004–2011

Detalhes bibliográficos
Autor(a) principal: Fumian, Tulio Machado
Data de Publicação: 2016
Outros Autores: Andrade, Juliana da Silva Ribeiro de, Leite, José Paulo Gagliardi, Miagostovich, Marize Pereira
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/16560
Resumo: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Comparada e Ambiental. Rio de Janeiro, RJ, Brasil.
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spelling Fumian, Tulio MachadoAndrade, Juliana da Silva Ribeiro deLeite, José Paulo GagliardiMiagostovich, Marize Pereira2016-12-26T14:22:45Z2016-12-26T14:22:45Z2016FUMIAN, Tulio Machado; et al. Norovirus Recombinant Strains Isolated from Gastroenteritis Outbreaks in Southern Brazil, 2004–2011. Plos One, v.11, n.4, e0145391, 12p, Apr. 2016.1932-6203https://www.arca.fiocruz.br/handle/icict/1656010.1371/journal.pone.0145391engPublic Library of ScienceGastroenteriteNorovirusSul do BrasilCepa recombinante de norovirusacute gastroenteritisNorovirus Recombinant StrainSouthern BrazilNorovirus Recombinant Strains Isolated from Gastroenteritis Outbreaks in Southern Brazil, 2004–2011info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Comparada e Ambiental. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Comparada e Ambiental. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Comparada e Ambiental. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Comparada e Ambiental. Rio de Janeiro, RJ, Brasil.Noroviruses are recognized as one of the leading causes of viral acute gastroenteritis, responsible for almost 50% of acute gastroenteritis outbreaks worldwide. The positive single-strand RNA genome of noroviruses presents a high mutation rate and these viruses are constantly evolving by nucleotide mutation and genome recombination. Norovirus recombinant strains have been detected as causing acute gastroenteritis outbreaks in several countries. However, in Brazil, only one report of a norovirus recombinant strain (GII.P7/GII.20) has been described in the northern region so far. For this study, 38 norovirus strains representative of outbreaks, 11 GII.4 and 27 non-GII.4, were randomly selected and amplified at the ORF1/ORF2 junction. Genetic recombination was identified by constructing phylogenetic trees of the polymerase and capsid genes, and further SimPlot and Bootscan analysis of the ORF1/ORF2 overlap. Sequence analysis revealed that 23 out of 27 (85%) non-GII.4 noroviruses were recombinant strains, characterized as: GII.P7/GII.6 (n = 9); GIIP.g/GII.12 (n = 4); GII.P16/GII.3 (n = 4); GII.Pe/GII.17 (n = 2); GII.P7/GII.14 (n = 1); GII.P13/GII.17 (n = 1); GII.P21/GII.3 (n = 1); and GII.P21/GII.13 (n = 1). On the other hand, among the GII.4 variants analyzed (Den Haag_2006b and New Orleans_2009) no recombination was observed. These data revealed the great diversity of norovirus recombinant strains associated with outbreaks, and describe for the first time these recombinant types circulating in Brazil. Our results obtained in southern Brazil corroborate the previous report for the northern region, demonstrating that norovirus recombinant strains are circulating more frequently than we expected. In addition, these results emphasize the relevance of including ORF1/ORF2-based analysis in surveillance studies as well as the importance of characterizing strains from other Brazilian regions to obtain epidemiological data for norovirus recombinant strains circulating in the country.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/16560/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALjuliana_andrade_etal_IOC_2016.pdfjuliana_andrade_etal_IOC_2016.pdfapplication/pdf1969643https://www.arca.fiocruz.br/bitstream/icict/16560/2/juliana_andrade_etal_IOC_2016.pdfdf814dc238ff8e34e22c2f7e11107106MD52TEXTjuliana_andrade_etal_IOC_2016.pdf.txtjuliana_andrade_etal_IOC_2016.pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv Norovirus Recombinant Strains Isolated from Gastroenteritis Outbreaks in Southern Brazil, 2004–2011
title Norovirus Recombinant Strains Isolated from Gastroenteritis Outbreaks in Southern Brazil, 2004–2011
spellingShingle Norovirus Recombinant Strains Isolated from Gastroenteritis Outbreaks in Southern Brazil, 2004–2011
Fumian, Tulio Machado
Gastroenterite
Norovirus
Sul do Brasil
Cepa recombinante de norovirus
acute gastroenteritis
Norovirus Recombinant Strain
Southern Brazil
title_short Norovirus Recombinant Strains Isolated from Gastroenteritis Outbreaks in Southern Brazil, 2004–2011
title_full Norovirus Recombinant Strains Isolated from Gastroenteritis Outbreaks in Southern Brazil, 2004–2011
title_fullStr Norovirus Recombinant Strains Isolated from Gastroenteritis Outbreaks in Southern Brazil, 2004–2011
title_full_unstemmed Norovirus Recombinant Strains Isolated from Gastroenteritis Outbreaks in Southern Brazil, 2004–2011
title_sort Norovirus Recombinant Strains Isolated from Gastroenteritis Outbreaks in Southern Brazil, 2004–2011
author Fumian, Tulio Machado
author_facet Fumian, Tulio Machado
Andrade, Juliana da Silva Ribeiro de
Leite, José Paulo Gagliardi
Miagostovich, Marize Pereira
author_role author
author2 Andrade, Juliana da Silva Ribeiro de
Leite, José Paulo Gagliardi
Miagostovich, Marize Pereira
author2_role author
author
author
dc.contributor.author.fl_str_mv Fumian, Tulio Machado
Andrade, Juliana da Silva Ribeiro de
Leite, José Paulo Gagliardi
Miagostovich, Marize Pereira
dc.subject.other.pt_BR.fl_str_mv Gastroenterite
Norovirus
Sul do Brasil
Cepa recombinante de norovirus
topic Gastroenterite
Norovirus
Sul do Brasil
Cepa recombinante de norovirus
acute gastroenteritis
Norovirus Recombinant Strain
Southern Brazil
dc.subject.en.pt_BR.fl_str_mv acute gastroenteritis
Norovirus Recombinant Strain
Southern Brazil
description Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Comparada e Ambiental. Rio de Janeiro, RJ, Brasil.
publishDate 2016
dc.date.accessioned.fl_str_mv 2016-12-26T14:22:45Z
dc.date.available.fl_str_mv 2016-12-26T14:22:45Z
dc.date.issued.fl_str_mv 2016
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.citation.fl_str_mv FUMIAN, Tulio Machado; et al. Norovirus Recombinant Strains Isolated from Gastroenteritis Outbreaks in Southern Brazil, 2004–2011. Plos One, v.11, n.4, e0145391, 12p, Apr. 2016.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/16560
dc.identifier.issn.pt_BR.fl_str_mv 1932-6203
dc.identifier.doi.none.fl_str_mv 10.1371/journal.pone.0145391
identifier_str_mv FUMIAN, Tulio Machado; et al. Norovirus Recombinant Strains Isolated from Gastroenteritis Outbreaks in Southern Brazil, 2004–2011. Plos One, v.11, n.4, e0145391, 12p, Apr. 2016.
1932-6203
10.1371/journal.pone.0145391
url https://www.arca.fiocruz.br/handle/icict/16560
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
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