Detection and quantification of Leishmania braziliensis in ectoparasites from dogs

Detalhes bibliográficos
Autor(a) principal: Morais, Rayana Carla Silva de
Data de Publicação: 2013
Outros Autores: Gonçalves-de-Albuquerque, Suênia da Cunha, Pessoa e Silva, Rômulo, Costa, Pietra Lemos, Silva, Kamila Gaudêncio da, Silva, Fernando José da, Brandão-Filho, Sinval Pinto, Dantas-Torres, Filipe, Paiva-Cavalcanti, Milena de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/23654
Resumo: Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de imunologia. Recife, PE, Brasil / Federal Rural University of Pernambuco. Recife, PE, Brazil.
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spelling Morais, Rayana Carla Silva deGonçalves-de-Albuquerque, Suênia da CunhaPessoa e Silva, RômuloCosta, Pietra LemosSilva, Kamila Gaudêncio daSilva, Fernando José daBrandão-Filho, Sinval PintoDantas-Torres, FilipePaiva-Cavalcanti, Milena de2017-12-14T11:31:34Z2017-12-14T11:31:34Z2013MORAIS, R. C. S. de et al. Detection and quantification of Leishmania braziliensis in ectoparasites from dogs. Veterinary Parasitology, v. 196, n. 3–4, p. 506–508, 23 set. 2013.1873-2550https://www.arca.fiocruz.br/handle/icict/2365410.1016/j.vetpar.2013.03.026engEctoparasitasCãesLeishmania braziliensisPCRPCR em tempo realEctoparasitesDogsLeishmania braziliensisPCRReal time PCRDoenças do CãoparasitologiaLeishmania braziliensisisolamento & purificaçãoFtirápterosparasitologiaSifonápterosparasitologiaCarrapatosparasitologiaAnimaisCãesDNA de Protozoárioisolamento & purificaçãoEctoparasitosesparasitologiaEctoparasitosesveterináriaDetection and quantification of Leishmania braziliensis in ectoparasites from dogsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de imunologia. Recife, PE, Brasil / Federal Rural University of Pernambuco. Recife, PE, Brazil.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de imunologia. Recife, PE, Brasil.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de imunologia. Recife, PE, Brasil.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de imunologia. Recife, PE, Brasil.Federal Rural University of Pernambuco. Recife, PE, Brazil.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de imunologia. Recife, PE, Brasil.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de imunologia. Recife, PE, Brasil.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de imunologia. Recife, PE, Brasil / University of Bari. Department of Veterinary Medicine. Bari, Italy.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de imunologia. Recife, PE, Brasil.American cutaneous leishmaniasis (ACL) is a disease caused by different species of Leishmania protozoa, Leishmania braziliensis being the main species found in Brazil. In this study, two rural areas in Pernambuco, northeastern Brazil, where ACL is endemic, were selected. Genomic DNA was extracted from canine ectoparasites (ticks, fleas, and lice) and tested using a conventional PCR and a quantitative real time PCR. A total of 117 ectoparasites were collected, being 50 (42.74%) of them positive for L. braziliensis (in at least one PCR protocol), with a mean parasite load of 14.14 fg/μL. Furthermore, 46 (92.00%) positive ectoparasites were collected from positive dogs and 4 (8.00%) from negative ones. This study reports the detection of L. braziliensis DNA in ectoparasites, but does not prove their vector competence. Certainly, experimental transmission studies are necessary to assess their role, if any, in the transmission of Leishmania parasites to dogs.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83092https://www.arca.fiocruz.br/bitstream/icict/23654/1/license.txt447f2497af1ef5cf99b5c07f6fa18dceMD51ORIGINALart. Detection and quantification - morais.pdfart. Detection and quantification - morais.pdfapplication/pdf475644https://www.arca.fiocruz.br/bitstream/icict/23654/2/art.%20Detection%20and%20quantification%20-%20morais.pdf056356e0ed02b22d240f00a1bdfbed23MD52TEXTart. Detection and quantification - morais.pdf.txtart. 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dc.title.pt_BR.fl_str_mv Detection and quantification of Leishmania braziliensis in ectoparasites from dogs
title Detection and quantification of Leishmania braziliensis in ectoparasites from dogs
spellingShingle Detection and quantification of Leishmania braziliensis in ectoparasites from dogs
Morais, Rayana Carla Silva de
Ectoparasitas
Cães
Leishmania braziliensis
PCR
PCR em tempo real
Ectoparasites
Dogs
Leishmania braziliensis
PCR
Real time PCR
Doenças do Cão
parasitologia
Leishmania braziliensis
isolamento & purificação
Ftirápteros
parasitologia
Sifonápteros
parasitologia
Carrapatos
parasitologia
Animais
Cães
DNA de Protozoário
isolamento & purificação
Ectoparasitoses
parasitologia
Ectoparasitoses
veterinária
title_short Detection and quantification of Leishmania braziliensis in ectoparasites from dogs
title_full Detection and quantification of Leishmania braziliensis in ectoparasites from dogs
title_fullStr Detection and quantification of Leishmania braziliensis in ectoparasites from dogs
title_full_unstemmed Detection and quantification of Leishmania braziliensis in ectoparasites from dogs
title_sort Detection and quantification of Leishmania braziliensis in ectoparasites from dogs
author Morais, Rayana Carla Silva de
author_facet Morais, Rayana Carla Silva de
Gonçalves-de-Albuquerque, Suênia da Cunha
Pessoa e Silva, Rômulo
Costa, Pietra Lemos
Silva, Kamila Gaudêncio da
Silva, Fernando José da
Brandão-Filho, Sinval Pinto
Dantas-Torres, Filipe
Paiva-Cavalcanti, Milena de
author_role author
author2 Gonçalves-de-Albuquerque, Suênia da Cunha
Pessoa e Silva, Rômulo
Costa, Pietra Lemos
Silva, Kamila Gaudêncio da
Silva, Fernando José da
Brandão-Filho, Sinval Pinto
Dantas-Torres, Filipe
Paiva-Cavalcanti, Milena de
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Morais, Rayana Carla Silva de
Gonçalves-de-Albuquerque, Suênia da Cunha
Pessoa e Silva, Rômulo
Costa, Pietra Lemos
Silva, Kamila Gaudêncio da
Silva, Fernando José da
Brandão-Filho, Sinval Pinto
Dantas-Torres, Filipe
Paiva-Cavalcanti, Milena de
dc.subject.other.pt_BR.fl_str_mv Ectoparasitas
Cães
Leishmania braziliensis
PCR
PCR em tempo real
topic Ectoparasitas
Cães
Leishmania braziliensis
PCR
PCR em tempo real
Ectoparasites
Dogs
Leishmania braziliensis
PCR
Real time PCR
Doenças do Cão
parasitologia
Leishmania braziliensis
isolamento & purificação
Ftirápteros
parasitologia
Sifonápteros
parasitologia
Carrapatos
parasitologia
Animais
Cães
DNA de Protozoário
isolamento & purificação
Ectoparasitoses
parasitologia
Ectoparasitoses
veterinária
dc.subject.en.pt_BR.fl_str_mv Ectoparasites
Dogs
Leishmania braziliensis
PCR
Real time PCR
dc.subject.decs.pt_BR.fl_str_mv Doenças do Cão
parasitologia
Leishmania braziliensis
isolamento & purificação
Ftirápteros
parasitologia
Sifonápteros
parasitologia
Carrapatos
parasitologia
Animais
Cães
DNA de Protozoário
isolamento & purificação
Ectoparasitoses
parasitologia
Ectoparasitoses
veterinária
description Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de imunologia. Recife, PE, Brasil / Federal Rural University of Pernambuco. Recife, PE, Brazil.
publishDate 2013
dc.date.issued.fl_str_mv 2013
dc.date.accessioned.fl_str_mv 2017-12-14T11:31:34Z
dc.date.available.fl_str_mv 2017-12-14T11:31:34Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv MORAIS, R. C. S. de et al. Detection and quantification of Leishmania braziliensis in ectoparasites from dogs. Veterinary Parasitology, v. 196, n. 3–4, p. 506–508, 23 set. 2013.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/23654
dc.identifier.issn.pt_BR.fl_str_mv 1873-2550
dc.identifier.doi.none.fl_str_mv 10.1016/j.vetpar.2013.03.026
identifier_str_mv MORAIS, R. C. S. de et al. Detection and quantification of Leishmania braziliensis in ectoparasites from dogs. Veterinary Parasitology, v. 196, n. 3–4, p. 506–508, 23 set. 2013.
1873-2550
10.1016/j.vetpar.2013.03.026
url https://www.arca.fiocruz.br/handle/icict/23654
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/23654/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/23654/2/art.%20Detection%20and%20quantification%20-%20morais.pdf
https://www.arca.fiocruz.br/bitstream/icict/23654/3/art.%20Detection%20and%20quantification%20-%20morais.pdf.txt
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