A New Technique Using Low Volumes: A New Technique to Assess the Molluscicidal Activity Using Low Volumes
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2017 |
| Outros Autores: | , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/23329 |
Resumo: | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Avaliação e Promoção da Saúde Ambiental. Rio de Janeiro, RJ. Brasil. |
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Santos, José Augusto AlbuquerqueCavalcante, Viviane PaixãoRangel, Leonardo da SilvaLeite, João Claudio Vitória AticoFaria, Robson Xavier2017-11-23T16:17:42Z2017-11-23T16:17:42Z2017SANTOS, José Augusto Albuquerque; et al. A New Technique Using Low Volumes: A New Technique to Assess the Molluscicidal Activity Using Low Volumes. Evidence-Based Complementary and Alternative Medicine, Volume 2017, Article ID 3673197, 10p, 2017.1741-427Xhttps://www.arca.fiocruz.br/handle/icict/2332910.1155/2017/36731971741-4288engHindawi Publishing CorporationEsquistossomoseAtividade MoluscicidaTécnicaEnsaios de BeckerBiomphalaria glabrataSchistosomiasisMolluscicidal ActivityTechniqueBecker’s assaysBiomphalaria glabrataA New Technique Using Low Volumes: A New Technique to Assess the Molluscicidal Activity Using Low Volumesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Avaliação e Promoção da Saúde Ambiental. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Avaliação e Promoção da Saúde Ambiental. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Avaliação e Promoção da Saúde Ambiental. Rio de Janeiro, RJ. Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Toxoplasmose e outras Protozooses. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Avaliação e Promoção da Saúde Ambiental. Rio de Janeiro, RJ. Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Toxoplasmose e outras Protozooses. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Toxoplasmose e outras Protozooses. Rio de Janeiro, RJ, Brasil.Schistosomiasis is a disease endemic in several states of Brazil. The population control of the transmitter mollusks is done with Bayluscide WP 70®, in the control programs. OMS preconize molluscicidal assays using Becker with 500 mL of final volume, restringing the number of natural products and synthetic drugs to be tested in function of high quantity of material necessary. A new technique to assess the toxic effects for Biomphalaria sp. is the purpose of this work, for developing adaptation for this aquatic organism, using a low volume of test solution in 24-well plates. We used Biomphalaria glabrata (10-12 mm size) in a static system, consisting of the following components: Becker containing 10 snails or 24-well plates where snails were individualized for a volume of 2 mL per well for 24 and 48 hours. For the assays, we added aqueous solutions of Bayluscide WP 70, at a concentration of 1-5 mg/L, distilled water, and 1% dimethyl sulfoxide. Data were evaluated using Kappa's coefficient, Z factor validation, and comparison study. This technique to assess the toxic effect has proven to be a useful tool to detect lethal and sublethal effects, which could be used as a new evaluation protocol.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/23329/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALjoseaugusto2_santos_etal_IOC_2017.pdfjoseaugusto2_santos_etal_IOC_2017.pdfapplication/pdf1262951https://www.arca.fiocruz.br/bitstream/icict/23329/2/joseaugusto2_santos_etal_IOC_2017.pdf173ab4c95af6da8d716fa382741e8601MD52TEXTjoseaugusto2_santos_etal_IOC_2017.pdf.txtjoseaugusto2_santos_etal_IOC_2017.pdf.txtExtracted texttext/plain41298https://www.arca.fiocruz.br/bitstream/icict/23329/3/joseaugusto2_santos_etal_IOC_2017.pdf.txt4d2173e252f21d8a34247239489d43e1MD53icict/233292018-08-15 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| dc.title.pt_BR.fl_str_mv |
A New Technique Using Low Volumes: A New Technique to Assess the Molluscicidal Activity Using Low Volumes |
| title |
A New Technique Using Low Volumes: A New Technique to Assess the Molluscicidal Activity Using Low Volumes |
| spellingShingle |
A New Technique Using Low Volumes: A New Technique to Assess the Molluscicidal Activity Using Low Volumes Santos, José Augusto Albuquerque Esquistossomose Atividade Moluscicida Técnica Ensaios de Becker Biomphalaria glabrata Schistosomiasis Molluscicidal Activity Technique Becker’s assays Biomphalaria glabrata |
| title_short |
A New Technique Using Low Volumes: A New Technique to Assess the Molluscicidal Activity Using Low Volumes |
| title_full |
A New Technique Using Low Volumes: A New Technique to Assess the Molluscicidal Activity Using Low Volumes |
| title_fullStr |
A New Technique Using Low Volumes: A New Technique to Assess the Molluscicidal Activity Using Low Volumes |
| title_full_unstemmed |
A New Technique Using Low Volumes: A New Technique to Assess the Molluscicidal Activity Using Low Volumes |
| title_sort |
A New Technique Using Low Volumes: A New Technique to Assess the Molluscicidal Activity Using Low Volumes |
| author |
Santos, José Augusto Albuquerque |
| author_facet |
Santos, José Augusto Albuquerque Cavalcante, Viviane Paixão Rangel, Leonardo da Silva Leite, João Claudio Vitória Atico Faria, Robson Xavier |
| author_role |
author |
| author2 |
Cavalcante, Viviane Paixão Rangel, Leonardo da Silva Leite, João Claudio Vitória Atico Faria, Robson Xavier |
| author2_role |
author author author author |
| dc.contributor.author.fl_str_mv |
Santos, José Augusto Albuquerque Cavalcante, Viviane Paixão Rangel, Leonardo da Silva Leite, João Claudio Vitória Atico Faria, Robson Xavier |
| dc.subject.other.pt_BR.fl_str_mv |
Esquistossomose Atividade Moluscicida Técnica Ensaios de Becker Biomphalaria glabrata |
| topic |
Esquistossomose Atividade Moluscicida Técnica Ensaios de Becker Biomphalaria glabrata Schistosomiasis Molluscicidal Activity Technique Becker’s assays Biomphalaria glabrata |
| dc.subject.en.pt_BR.fl_str_mv |
Schistosomiasis Molluscicidal Activity Technique Becker’s assays Biomphalaria glabrata |
| description |
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Avaliação e Promoção da Saúde Ambiental. Rio de Janeiro, RJ. Brasil. |
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2017 |
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2017-11-23T16:17:42Z |
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2017-11-23T16:17:42Z |
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2017 |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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SANTOS, José Augusto Albuquerque; et al. A New Technique Using Low Volumes: A New Technique to Assess the Molluscicidal Activity Using Low Volumes. Evidence-Based Complementary and Alternative Medicine, Volume 2017, Article ID 3673197, 10p, 2017. |
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https://www.arca.fiocruz.br/handle/icict/23329 |
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1741-427X |
| dc.identifier.doi.none.fl_str_mv |
10.1155/2017/3673197 |
| dc.identifier.eissn.none.fl_str_mv |
1741-4288 |
| identifier_str_mv |
SANTOS, José Augusto Albuquerque; et al. A New Technique Using Low Volumes: A New Technique to Assess the Molluscicidal Activity Using Low Volumes. Evidence-Based Complementary and Alternative Medicine, Volume 2017, Article ID 3673197, 10p, 2017. 1741-427X 10.1155/2017/3673197 1741-4288 |
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Hindawi Publishing Corporation |
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Hindawi Publishing Corporation |
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