Genomic analysis of listeria monocytogenes strains isolated from food and clinical samples

Detalhes bibliográficos
Autor(a) principal: Almeida, R. M.
Data de Publicação: 2021
Outros Autores: Junqueira, R. M., Panzenhagen, P. H. N., Conte Júnior, C. A., Pereira, R. C. L., Reis, R. C. L., Hofer, C. B., Vallim, D. C.
Tipo de documento: Artigo de conferência
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/52427
Resumo: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Zoonoses Bacterianas. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Hospital Universitário Clementino Fraga Filho. Rio de Janeiro, RJ, Bras
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spelling Almeida, R. M.Junqueira, R. M.Panzenhagen, P. H. N.Conte Júnior, C. A.Pereira, R. C. L.Reis, R. C. L.Hofer, C. B.Vallim, D. C.2022-04-27T13:01:28Z2022-04-27T13:01:28Z2021ALMEIDA, R. M. et al. Genomic analysis of listeria monocytogenes strains isolated from food and clinical samples. In: SEMINÁRIO INTERNACIONAL DO PROGRAMA DE PÓS-GRADUAÇÃO EM DOENÇAS INFECCIOSAS E PARASITÁRIAS, 2., 2021, Rio de Janeiro. Cadernos de Resumos... Rio de Janeiro: PPGDIC/Faculdade de Medicina/UFRJ, 2021. 9 p.https://www.arca.fiocruz.br/handle/icict/52427engListerioseListeria MonocytogenesWGSListeria MonocytogenesClonal ComplexesOne HealthGenomic analysis of listeria monocytogenes strains isolated from food and clinical samplesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Zoonoses Bacterianas. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Hospital Universitário Clementino Fraga Filho. Rio de Janeiro, RJ, BrasFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Zoonoses Bacterianas. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Química. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Química. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Zoonoses Bacterianas. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Zoonoses Bacterianas. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Hospital Universitário Clementino Fraga Filho. Rio de Janeiro, RJ, BrasFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Zoonoses Bacterianas. Rio de Janeiro, RJ, Brasil.Introduction: Listeriosis is a serious foodborne illness in high-risk groups such as the elderly, newborns and pregnant women. However, the ecology of Listeria monocytogenes is poorly understood and some strains capacity to adapt to environmental conditions, what makes Lm a major challenge to food safety in the industry. Objective: The aim of this study was to analyze, by Whole Genome Sequencing (WGS), 48 strains of Lm isolated from food (21) and clinical samples (27), from 1975 to 2014 and different States. Method: In silico analysis was performed using tools built in the BIGSdb-Lm database of the Institute Pasteur/France. Result: MLST analysis showed the presence of Clonal Complexes: CC3 (15), CC1 (13), CC218 (9), CC9 (5), CC2 (2), CC7 (1), CC59 (1), CC155 (1) and CC315 (1), corresponding to ST3 (14), ST1 (13), ST218 (9), ST9 (4), ST2 (2), ST7 (1), ST59 (1), ST122 (1), ST520 (1), ST1434 (1) and ST1435 (1) respectively. In addition, two new STs were described, ST1434 and ST1435 isolated from ricotta cheese and human blood, respectively. ST1 was associated with hypervirulence and tropism towards the central nervous system (CNS) and ST9 was strongly associated with food but not clinical samples. Plasmids were found in 23 strains, six of them with the bcrA gene, which confers resistance to benzylkonium chloride. In the chromosome, genes related to resistance against heavy metals (mco (6), mdrL/yfmO (27), merR1 (5), merR2 (5)) and antibiotics (aacA4 (5), fosX (48), sul (46), mprF (48), mdrL/yfmO (27), norB (48), lde (48)) were also detected. Conclusion: WGS allowed a deeper analysis of the genetic diversity of Lm circulating in Brazil, contributing to a better understanding of its virulence and resistance patterns. The sequences are still being analyzed, so patterns and proportions observed so far may be subject to change.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83132https://www.arca.fiocruz.br/bitstream/icict/52427/1/license.txt70425d8cb6e40fdcf4edd2ef0660b45bMD51ORIGINAL2.pdf2.pdfapplication/pdf908745https://www.arca.fiocruz.br/bitstream/icict/52427/2/2.pdf586d817d97be4743374138a82d9d51b7MD52icict/524272022-04-27 17:42:59.982oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352022-04-27T20:42:59Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Genomic analysis of listeria monocytogenes strains isolated from food and clinical samples
title Genomic analysis of listeria monocytogenes strains isolated from food and clinical samples
spellingShingle Genomic analysis of listeria monocytogenes strains isolated from food and clinical samples
Almeida, R. M.
Listeriose
Listeria Monocytogenes
WGS
Listeria Monocytogenes
Clonal Complexes
One Health
title_short Genomic analysis of listeria monocytogenes strains isolated from food and clinical samples
title_full Genomic analysis of listeria monocytogenes strains isolated from food and clinical samples
title_fullStr Genomic analysis of listeria monocytogenes strains isolated from food and clinical samples
title_full_unstemmed Genomic analysis of listeria monocytogenes strains isolated from food and clinical samples
title_sort Genomic analysis of listeria monocytogenes strains isolated from food and clinical samples
author Almeida, R. M.
author_facet Almeida, R. M.
Junqueira, R. M.
Panzenhagen, P. H. N.
Conte Júnior, C. A.
Pereira, R. C. L.
Reis, R. C. L.
Hofer, C. B.
Vallim, D. C.
author_role author
author2 Junqueira, R. M.
Panzenhagen, P. H. N.
Conte Júnior, C. A.
Pereira, R. C. L.
Reis, R. C. L.
Hofer, C. B.
Vallim, D. C.
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Almeida, R. M.
Junqueira, R. M.
Panzenhagen, P. H. N.
Conte Júnior, C. A.
Pereira, R. C. L.
Reis, R. C. L.
Hofer, C. B.
Vallim, D. C.
dc.subject.other.pt_BR.fl_str_mv Listeriose
Listeria Monocytogenes
topic Listeriose
Listeria Monocytogenes
WGS
Listeria Monocytogenes
Clonal Complexes
One Health
dc.subject.en.pt_BR.fl_str_mv WGS
Listeria Monocytogenes
Clonal Complexes
One Health
description Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Zoonoses Bacterianas. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Hospital Universitário Clementino Fraga Filho. Rio de Janeiro, RJ, Bras
publishDate 2021
dc.date.issued.fl_str_mv 2021
dc.date.accessioned.fl_str_mv 2022-04-27T13:01:28Z
dc.date.available.fl_str_mv 2022-04-27T13:01:28Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/conferenceObject
format conferenceObject
status_str publishedVersion
dc.identifier.citation.fl_str_mv ALMEIDA, R. M. et al. Genomic analysis of listeria monocytogenes strains isolated from food and clinical samples. In: SEMINÁRIO INTERNACIONAL DO PROGRAMA DE PÓS-GRADUAÇÃO EM DOENÇAS INFECCIOSAS E PARASITÁRIAS, 2., 2021, Rio de Janeiro. Cadernos de Resumos... Rio de Janeiro: PPGDIC/Faculdade de Medicina/UFRJ, 2021. 9 p.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/52427
identifier_str_mv ALMEIDA, R. M. et al. Genomic analysis of listeria monocytogenes strains isolated from food and clinical samples. In: SEMINÁRIO INTERNACIONAL DO PROGRAMA DE PÓS-GRADUAÇÃO EM DOENÇAS INFECCIOSAS E PARASITÁRIAS, 2., 2021, Rio de Janeiro. Cadernos de Resumos... Rio de Janeiro: PPGDIC/Faculdade de Medicina/UFRJ, 2021. 9 p.
url https://www.arca.fiocruz.br/handle/icict/52427
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/52427/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/52427/2/2.pdf
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