Screening Aedes aegypti (Diptera: Culicidae) Populations From Pernambuco, Brazil for Resistance to Temephos, Diflubenzuron, and Cypermethrin and Characterization of Potential Resistance Mechanisms
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2019 |
| Outros Autores: | , , , , , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/35845 |
Resumo: | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Entomologia. Recife, PE, Brasil. |
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Araújo, Ana Paula dePaiva, Marcelo Henrique SantosCabral, Amanda MariaCavalcanti, Antônio Emanuel Holanda DiasPessoa, Luiz Fernando FreitasDiniz, Diego Felipe AraujoHelvecio, ElisamaSilva, Ellyda Vanessa Gomes daSilva, Norma Machado daAnastácio, Daniela BandeiraPontes, ClaudeniceNunes, VâniaSouza, Maria de Fátima Marinho deMagalhães, Fernando Jorge RodriguesSantos, Maria Alice Varjal de MeloAyres, Constância Flávia Junqueira2019-09-24T14:23:47Z2019-09-24T14:23:47Z2019ARAÚJO, A. P. de et al. Screening Aedes aegypti (Diptera: Culicidae) Populations From Pernambuco, Brazil for Resistance to Temephos, Diflubenzuron, and Cypermethrin and Characterization of Potential Resistance Mechanisms. Journal of Insect Science, v. 19, n. 3, p. 1-15, 2019.1536-2442https://www.arca.fiocruz.br/handle/icict/3584510.1093/jisesa/iez054engScreening Aedes aegypti (Diptera: Culicidae) Populations From Pernambuco, Brazil for Resistance to Temephos, Diflubenzuron, and Cypermethrin and Characterization of Potential Resistance Mechanismsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Entomologia. Recife, PE, Brasil.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Entomologia. Recife, PE, Brasil / Universidade Federal de Pernambuco. Centro Acadêmico do Agreste. Núcleo de Ciências da Vida. Caruaru, PE, Brasil.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Entomologia. Recife, PE, Brasil.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Entomologia. Recife, PE, Brasil.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Entomologia. Recife, PE, Brasil.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Entomologia. Recife, PE, Brasil.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Entomologia. Recife, PE, Brasil.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Entomologia. Recife, PE, Brasil.Universidade Federal de Santa Catarina. Departamento de Biologia Celular, Embriologia e Genética – BEG. Florianópolis, SC, Brasil.Departamento de Saúde de Pernambuco. Recife, PE, Brasil.Departamento de Saúde de Pernambuco. Recife, PE, Brasil.Secretaria Municipal de Saúde do Recife, Recife, PE, Brasil.Secretaria de Saúde do Distrito de Fernando de Noronha. Fernando de Noronha, PE, Brasil.Secretaria de Saúde do Distrito de Fernando de Noronha. Fernando de Noronha, PE, Brasil.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Entomologia. Recife, PE, Brasil.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Entomologia. Recife, PE, Brasil.Resistance to chemical insecticides detected in Aedes aegypti (L.) mosquitoes has been a problem for the National Dengue Control Program (PNCD) over the last years. In order to provide deeper knowledge of resistance to xenobiotics, our study evaluated the susceptibility profile of temephos, diflubenzuron, and cypermethrin insecticides in natural mosquito populations from the Pernambuco State, associating these results with the local historical use of such compounds. Furthermore, mechanisms that may be associated with this particular type of resistance were characterized. Bioassays with multiple temephos and diflubenzuron concentrations were performed to detect and quantify resistance. For cypermethrin, diagnostic dose assays were performed. Biochemical tests were carried out to quantify the activity of detoxification enzymes. In addition, a screening of mutations present in the voltage-gated sodium channel gene (NaV) was performed in samples previously submitted to bioassays with cypermethrin. The populations under study were resistant to temephos and showed a positive correlation between insecticide consumption and the resistance ratio (RR) to the compound. For diflubenzuron, the biological activity ratio (BAR) ranged from 1.3 to 4.7 times, when compared to the susceptible strain. All populations showed resistance to cypermethrin. Altered enzymatic profiles of alpha, p-nitrophenyl acetate (PNPA) esterases and glutathione-S-transferases were recorded in most of these samples. Molecular analysis demonstrated that Arcoverde was the only population that presented the mutated form 1016Ile/Ile. These findings show that the situation is critical vis-à-vis the effectiveness of mosquito control using chemical insecticides, since resistance to temephos and cypermethrin is widespread in Ae. aegypti from Pernambuco.Insecticide resistanceChemical controlMosquito controlAedesAnimaisDiflubenzuronFemininoResistência a InseticidasGenéticaInseticidasLarvaMasculinoPiretrinasTemefósTestes de ToxicidadeCanais de Sódio Disparados por Voltageminfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83085https://www.arca.fiocruz.br/bitstream/icict/35845/1/license.txt4194663c61da82c21746deb8d6361188MD51ORIGINALScreening Aedes aegypti.pdfScreening Aedes aegypti.pdfapplication/pdf320276https://www.arca.fiocruz.br/bitstream/icict/35845/2/Screening%20Aedes%20aegypti.pdf95794516356ff200a7bad3d9123f0aa3MD52TEXTScreening Aedes aegypti.pdf.txtScreening Aedes 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| dc.title.pt_BR.fl_str_mv |
Screening Aedes aegypti (Diptera: Culicidae) Populations From Pernambuco, Brazil for Resistance to Temephos, Diflubenzuron, and Cypermethrin and Characterization of Potential Resistance Mechanisms |
| title |
Screening Aedes aegypti (Diptera: Culicidae) Populations From Pernambuco, Brazil for Resistance to Temephos, Diflubenzuron, and Cypermethrin and Characterization of Potential Resistance Mechanisms |
| spellingShingle |
Screening Aedes aegypti (Diptera: Culicidae) Populations From Pernambuco, Brazil for Resistance to Temephos, Diflubenzuron, and Cypermethrin and Characterization of Potential Resistance Mechanisms Araújo, Ana Paula de Insecticide resistance Chemical control Mosquito control Aedes Animais Diflubenzuron Feminino Resistência a Inseticidas Genética Inseticidas Larva Masculino Piretrinas Temefós Testes de Toxicidade Canais de Sódio Disparados por Voltagem |
| title_short |
Screening Aedes aegypti (Diptera: Culicidae) Populations From Pernambuco, Brazil for Resistance to Temephos, Diflubenzuron, and Cypermethrin and Characterization of Potential Resistance Mechanisms |
| title_full |
Screening Aedes aegypti (Diptera: Culicidae) Populations From Pernambuco, Brazil for Resistance to Temephos, Diflubenzuron, and Cypermethrin and Characterization of Potential Resistance Mechanisms |
| title_fullStr |
Screening Aedes aegypti (Diptera: Culicidae) Populations From Pernambuco, Brazil for Resistance to Temephos, Diflubenzuron, and Cypermethrin and Characterization of Potential Resistance Mechanisms |
| title_full_unstemmed |
Screening Aedes aegypti (Diptera: Culicidae) Populations From Pernambuco, Brazil for Resistance to Temephos, Diflubenzuron, and Cypermethrin and Characterization of Potential Resistance Mechanisms |
| title_sort |
Screening Aedes aegypti (Diptera: Culicidae) Populations From Pernambuco, Brazil for Resistance to Temephos, Diflubenzuron, and Cypermethrin and Characterization of Potential Resistance Mechanisms |
| author |
Araújo, Ana Paula de |
| author_facet |
Araújo, Ana Paula de Paiva, Marcelo Henrique Santos Cabral, Amanda Maria Cavalcanti, Antônio Emanuel Holanda Dias Pessoa, Luiz Fernando Freitas Diniz, Diego Felipe Araujo Helvecio, Elisama Silva, Ellyda Vanessa Gomes da Silva, Norma Machado da Anastácio, Daniela Bandeira Pontes, Claudenice Nunes, Vânia Souza, Maria de Fátima Marinho de Magalhães, Fernando Jorge Rodrigues Santos, Maria Alice Varjal de Melo Ayres, Constância Flávia Junqueira |
| author_role |
author |
| author2 |
Paiva, Marcelo Henrique Santos Cabral, Amanda Maria Cavalcanti, Antônio Emanuel Holanda Dias Pessoa, Luiz Fernando Freitas Diniz, Diego Felipe Araujo Helvecio, Elisama Silva, Ellyda Vanessa Gomes da Silva, Norma Machado da Anastácio, Daniela Bandeira Pontes, Claudenice Nunes, Vânia Souza, Maria de Fátima Marinho de Magalhães, Fernando Jorge Rodrigues Santos, Maria Alice Varjal de Melo Ayres, Constância Flávia Junqueira |
| author2_role |
author author author author author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Araújo, Ana Paula de Paiva, Marcelo Henrique Santos Cabral, Amanda Maria Cavalcanti, Antônio Emanuel Holanda Dias Pessoa, Luiz Fernando Freitas Diniz, Diego Felipe Araujo Helvecio, Elisama Silva, Ellyda Vanessa Gomes da Silva, Norma Machado da Anastácio, Daniela Bandeira Pontes, Claudenice Nunes, Vânia Souza, Maria de Fátima Marinho de Magalhães, Fernando Jorge Rodrigues Santos, Maria Alice Varjal de Melo Ayres, Constância Flávia Junqueira |
| dc.subject.en.pt_BR.fl_str_mv |
Insecticide resistance Chemical control Mosquito control |
| topic |
Insecticide resistance Chemical control Mosquito control Aedes Animais Diflubenzuron Feminino Resistência a Inseticidas Genética Inseticidas Larva Masculino Piretrinas Temefós Testes de Toxicidade Canais de Sódio Disparados por Voltagem |
| dc.subject.decs.pt_BR.fl_str_mv |
Aedes Animais Diflubenzuron Feminino Resistência a Inseticidas Genética Inseticidas Larva Masculino Piretrinas Temefós Testes de Toxicidade Canais de Sódio Disparados por Voltagem |
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Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Entomologia. Recife, PE, Brasil. |
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2019 |
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2019-09-24T14:23:47Z |
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2019-09-24T14:23:47Z |
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2019 |
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info:eu-repo/semantics/article |
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ARAÚJO, A. P. de et al. Screening Aedes aegypti (Diptera: Culicidae) Populations From Pernambuco, Brazil for Resistance to Temephos, Diflubenzuron, and Cypermethrin and Characterization of Potential Resistance Mechanisms. Journal of Insect Science, v. 19, n. 3, p. 1-15, 2019. |
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https://www.arca.fiocruz.br/handle/icict/35845 |
| dc.identifier.issn.pt_BR.fl_str_mv |
1536-2442 |
| dc.identifier.doi.none.fl_str_mv |
10.1093/jisesa/iez054 |
| identifier_str_mv |
ARAÚJO, A. P. de et al. Screening Aedes aegypti (Diptera: Culicidae) Populations From Pernambuco, Brazil for Resistance to Temephos, Diflubenzuron, and Cypermethrin and Characterization of Potential Resistance Mechanisms. Journal of Insect Science, v. 19, n. 3, p. 1-15, 2019. 1536-2442 10.1093/jisesa/iez054 |
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