The MHC gene region of murine hosts influences the differential tissue tropism of infecting Trypanosoma cruzi strains.

Detalhes bibliográficos
Autor(a) principal: Freitas, Jorge Marcelo de
Data de Publicação: 2009
Outros Autores: Andrade, Luciana de Oliveira, Pires, Simone da Fonseca, Lima, Ricardo Santana de, Chiari, Égler, Santos, Ricardo Ribeiro dos, Soares, Milena Botelho Pereira, Machado, Carlos Renato, Franco, Gloria Regina, Pena, Sergio Danilo Junho, Macedo, Andréa Mara
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/9024
Resumo: Universidade Federal de Minas Gerais. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brasil
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spelling Freitas, Jorge Marcelo deAndrade, Luciana de OliveiraPires, Simone da FonsecaLima, Ricardo Santana deChiari, ÉglerSantos, Ricardo Ribeiro dosSoares, Milena Botelho PereiraMachado, Carlos RenatoFranco, Gloria ReginaPena, Sergio Danilo JunhoMacedo, Andréa Mara2014-12-01T19:21:59Z2014-12-01T19:21:59Z2009FREITAS, J. M. et al. The MHC gene region of murine hosts influences the differential tissue tropism of infecting Trypanosoma cruzi strains. PLoS One, v. 4, n. 4, p. e5113, 2009.1932-6203https://www.arca.fiocruz.br/handle/icict/902410.1371/journal.pone.0005113engPublic Library of ScienceThe MHC gene region of murine hosts influences the differential tissue tropism of infecting Trypanosoma cruzi strains.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal de Minas Gerais. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, BrasilUniversidade Federal de Minas Gerais. Departamento de Morfologia. Belo Horizonte, MG, BrasilUniversidade Federal de Minas Gerais. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilUniversidade Federal de Minas Gerais. Departamento de Parasitologia. Belo Horizonte, MG, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilUniversidade Federal de Minas Gerais. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, BrasilUniversidade Federal de Minas Gerais. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, BrasilUniversidade Federal de Minas Gerais. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, BrasilUniversidade Federal de Minas Gerais. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, BrasilWe have previously demonstrated that both parasite genetic variability and host genetic background were important in determining the differential tissue distribution of the Col1.7G2 and JG T. cruzi monoclonal strains after artificial infections in mice. We observed that the JG strain was most prevalent in hearts of mouse lineages with the MHC haplotype H-2(d) (BALB/c and DBA2), while Col1.7G2 was predominant in hearts from C57BL/6 mice, which have the H-2(b) haplotype. To assess whether the MHC gene region indeed influenced tissue tropism of T. cruzi, we used the same two parasite strains to infect C57BL/6 (H-2(b)) and C57BLKS/J (H-2(d)) mice; the latter strain results from the introgression of DBA2 MHC region into the C57BL/6 background. We also performed ex vivo infections of cardiac explants from four congenic mice lineages with the H-2(b) and H-2(d) haplotypes arranged in two different genetic backgrounds: C57BLKS/J (H-2(d)) versus C57BL/6 (H-2(b)) and BALB/c (H-2(d)) versus BALB/B10-H2(b) (H-2(b)). In agreement with our former observations, Col1.7G2 was predominant in hearts from C57BL/6 mice (H-2(b)), but we observed a clear predominance of the JG strain in hearts from C57BLKS/J animals (H-2(d)). In the ex vivo experiments Col1.7G2 also prevailed in explants from H-2(b) animals while no predominance of any of the strains was observed in H-2(d) mice explants, regardless of the genetic background. These observations clearly demonstrate that the MHC region influences the differential tissue distribution pattern of infecting T. cruzi strains, which by its turn may be in a human infection the determinant for the clinical forms of the Chagas disease.Interações Hospedeiro-Parasita/genéticaComplexo Principal de Histocompatibilidade/genéticaTropismoTrypanosoma cruzi/patogenicidadeAnimaisSequência de BasesPrimers do DNAPrimers do DNACamundongosCamundongos Endogâmicos BALB CCamundongos Endogâmicos C57BLEspecificidade de ÓrgãosReação em Cadeia da Polimeraseinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://www.arca.fiocruz.br/bitstream/icict/9024/1/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD51ORIGINALFreitas J M The MHC Gene....pdfFreitas J M The MHC Gene....pdfapplication/pdf278759https://www.arca.fiocruz.br/bitstream/icict/9024/2/Freitas%20J%20M%20The%20MHC%20Gene....pdffb3a241aa2cf18995132f019f21c7f4cMD52TEXTFreitas J M The MHC Gene....pdf.txtFreitas J M The MHC Gene....pdf.txtExtracted texttext/plain29608https://www.arca.fiocruz.br/bitstream/icict/9024/3/Freitas%20J%20M%20The%20MHC%20Gene....pdf.txtce172a2b26a45b8fb681b70bfbf55f84MD53icict/90242023-03-15 14:33:02.432oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-15T17:33:02Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv The MHC gene region of murine hosts influences the differential tissue tropism of infecting Trypanosoma cruzi strains.
title The MHC gene region of murine hosts influences the differential tissue tropism of infecting Trypanosoma cruzi strains.
spellingShingle The MHC gene region of murine hosts influences the differential tissue tropism of infecting Trypanosoma cruzi strains.
Freitas, Jorge Marcelo de
Interações Hospedeiro-Parasita/genética
Complexo Principal de Histocompatibilidade/genética
Tropismo
Trypanosoma cruzi/patogenicidade
Animais
Sequência de Bases
Primers do DNA
Primers do DNA
Camundongos
Camundongos Endogâmicos BALB C
Camundongos Endogâmicos C57BL
Especificidade de Órgãos
Reação em Cadeia da Polimerase
title_short The MHC gene region of murine hosts influences the differential tissue tropism of infecting Trypanosoma cruzi strains.
title_full The MHC gene region of murine hosts influences the differential tissue tropism of infecting Trypanosoma cruzi strains.
title_fullStr The MHC gene region of murine hosts influences the differential tissue tropism of infecting Trypanosoma cruzi strains.
title_full_unstemmed The MHC gene region of murine hosts influences the differential tissue tropism of infecting Trypanosoma cruzi strains.
title_sort The MHC gene region of murine hosts influences the differential tissue tropism of infecting Trypanosoma cruzi strains.
author Freitas, Jorge Marcelo de
author_facet Freitas, Jorge Marcelo de
Andrade, Luciana de Oliveira
Pires, Simone da Fonseca
Lima, Ricardo Santana de
Chiari, Égler
Santos, Ricardo Ribeiro dos
Soares, Milena Botelho Pereira
Machado, Carlos Renato
Franco, Gloria Regina
Pena, Sergio Danilo Junho
Macedo, Andréa Mara
author_role author
author2 Andrade, Luciana de Oliveira
Pires, Simone da Fonseca
Lima, Ricardo Santana de
Chiari, Égler
Santos, Ricardo Ribeiro dos
Soares, Milena Botelho Pereira
Machado, Carlos Renato
Franco, Gloria Regina
Pena, Sergio Danilo Junho
Macedo, Andréa Mara
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Freitas, Jorge Marcelo de
Andrade, Luciana de Oliveira
Pires, Simone da Fonseca
Lima, Ricardo Santana de
Chiari, Égler
Santos, Ricardo Ribeiro dos
Soares, Milena Botelho Pereira
Machado, Carlos Renato
Franco, Gloria Regina
Pena, Sergio Danilo Junho
Macedo, Andréa Mara
dc.subject.decs.pt_BR.fl_str_mv Interações Hospedeiro-Parasita/genética
Complexo Principal de Histocompatibilidade/genética
Tropismo
Trypanosoma cruzi/patogenicidade
Animais
Sequência de Bases
Primers do DNA
Primers do DNA
Camundongos
Camundongos Endogâmicos BALB C
Camundongos Endogâmicos C57BL
Especificidade de Órgãos
Reação em Cadeia da Polimerase
topic Interações Hospedeiro-Parasita/genética
Complexo Principal de Histocompatibilidade/genética
Tropismo
Trypanosoma cruzi/patogenicidade
Animais
Sequência de Bases
Primers do DNA
Primers do DNA
Camundongos
Camundongos Endogâmicos BALB C
Camundongos Endogâmicos C57BL
Especificidade de Órgãos
Reação em Cadeia da Polimerase
description Universidade Federal de Minas Gerais. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brasil
publishDate 2009
dc.date.issued.fl_str_mv 2009
dc.date.accessioned.fl_str_mv 2014-12-01T19:21:59Z
dc.date.available.fl_str_mv 2014-12-01T19:21:59Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv FREITAS, J. M. et al. The MHC gene region of murine hosts influences the differential tissue tropism of infecting Trypanosoma cruzi strains. PLoS One, v. 4, n. 4, p. e5113, 2009.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/9024
dc.identifier.issn.none.fl_str_mv 1932-6203
dc.identifier.doi.none.fl_str_mv 10.1371/journal.pone.0005113
identifier_str_mv FREITAS, J. M. et al. The MHC gene region of murine hosts influences the differential tissue tropism of infecting Trypanosoma cruzi strains. PLoS One, v. 4, n. 4, p. e5113, 2009.
1932-6203
10.1371/journal.pone.0005113
url https://www.arca.fiocruz.br/handle/icict/9024
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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