Comparative study of in situ hybridization, immunohistochemistry and parasitological culture for the diagnosis of canine leishmaniosis
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2015 |
| Outros Autores: | , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/31416 |
Resumo: | Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Pesquisa Clínica em Dermatozoonoses em Animais Domésticos. Rio de Janeiro, RJ, Brasil / Michigan State University. College of Veterinary Medicine. Department of Pathobiology and Diagnostic Investigation. Lansing, MI, USA. |
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Furtado, Marina C.Menezes, Rodrigo C.Kiupel, MattiMadeira, Maria F.Oliveira, Raquel V. C.Langohr, Ingeborg M.Figueiredo, Fabiano B.2019-02-05T12:48:29Z2019-02-05T12:48:29Z2015FURTADO, Marina C. et al. Comparative study of in situ hybridization, immunohistochemistry and parasitological culture for the diagnosis of canine leishmaniosis. Parasites and Vectors, v. 8, n. 620, p. 1-5, 2015.1756-3305https://www.arca.fiocruz.br/handle/icict/3141610.1186/s13071-015-1224-41756-3305engBMCComparative study of in situ hybridization, immunohistochemistry and parasitological culture for the diagnosis of canine leishmaniosisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Pesquisa Clínica em Dermatozoonoses em Animais Domésticos. Rio de Janeiro, RJ, Brasil / Michigan State University. College of Veterinary Medicine. Department of Pathobiology and Diagnostic Investigation. Lansing, MI, USA.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Pesquisa Clínica em Dermatozoonoses em Animais Domésticos. Rio de Janeiro, RJ, Brasil.Michigan State University. College of Veterinary Medicine. Department of Pathobiology and Diagnostic Investigation. Lansing, MI, USA.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Vigilância em Leishmanioses. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Epidemiologia Clínica. Rio de Janeiro, RJ, Brasil.Michigan State University. College of Veterinary Medicine. Department of Pathobiology and Diagnostic Investigation. Lansing, MI, USA / Louisiana State University. School of Veterinary Medicine. Department of Pathobiological Sciences. Baton Rouge, LA, USA.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Pesquisa Clínica em Dermatozoonoses em Animais Domésticos. Rio de Janeiro, RJ, Brasil.Background: The establishment of an accurate diagnostic protocol for canine visceral leishmaniosis (CanL) is a significant laboratory challenge and the lack of a reliable reference standard is one of the major problems. The aim of this study was to compare in situ hybridization (ISH), immunohistochemistry (IHC) and parasitological culture (PC) for detection of L. infantum in skin, spleen, lymph node and bone marrow of clinically healthy and sick seropositive dogs. Findings: The study included 65 dogs positive with both DPP® and ELISA for anti-Leishmania antibodies. In situ hybridization of spleen or lymph node had the highest positivity rates of L. infantum detection. The total positivity rates for IHC, ISH and PC were 70 %, 68.1 % and 65.8 %, respectively. When combining techniques, the positivity rates were 81.5 % in the spleen, 79.0 % in lymph nodes, 59.0 % in bone marrow and 52.3 % in the skin. The highest percentage of infected dogs (87.7 %) was detected by using lymph node samples. When examining only skin, positivity was significantly higher in sick dogs than in the clinically healthy dogs. Infection with L. infantum was confirmed in 95.8 % of sick dogs and in 82.4 % of healthy dogs. Conclusions: Considering the advantages of accurately diagnosing different Leishmania species and of being more sensitive than PC, ISH should be considered as reference standard test for the diagnosis of CanL. Spleen and lymph node are the most suitable tissues to confirm infection with L. infantum in seropositive dogs. The testing of only skin from clinically healthy dogs may result in a high percentage of false negative results.Leishmania infantumDogDiagnosisIn situ hybridizationImmunohistochemistryParasitological cultureinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83100https://www.arca.fiocruz.br/bitstream/icict/31416/1/license.txte0c0e718db411cbd1e3f91252b389ec2MD51ORIGINALve_Furtado_Marina _etal_INI_2015.pdfve_Furtado_Marina _etal_INI_2015.pdfapplication/pdf1124956https://www.arca.fiocruz.br/bitstream/icict/31416/2/ve_Furtado_Marina%20_etal_INI_2015.pdf1d5fb7c79636cde15f45eebd3fac5cc7MD52TEXTve_Furtado_Marina _etal_INI_2015.pdf.txtve_Furtado_Marina _etal_INI_2015.pdf.txtExtracted texttext/plain23156https://www.arca.fiocruz.br/bitstream/icict/31416/3/ve_Furtado_Marina%20_etal_INI_2015.pdf.txt3418edefa9cd15100a9e00e1dedf7877MD53icict/314162023-08-07 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| dc.title.pt_BR.fl_str_mv |
Comparative study of in situ hybridization, immunohistochemistry and parasitological culture for the diagnosis of canine leishmaniosis |
| title |
Comparative study of in situ hybridization, immunohistochemistry and parasitological culture for the diagnosis of canine leishmaniosis |
| spellingShingle |
Comparative study of in situ hybridization, immunohistochemistry and parasitological culture for the diagnosis of canine leishmaniosis Furtado, Marina C. Leishmania infantum Dog Diagnosis In situ hybridization Immunohistochemistry Parasitological culture |
| title_short |
Comparative study of in situ hybridization, immunohistochemistry and parasitological culture for the diagnosis of canine leishmaniosis |
| title_full |
Comparative study of in situ hybridization, immunohistochemistry and parasitological culture for the diagnosis of canine leishmaniosis |
| title_fullStr |
Comparative study of in situ hybridization, immunohistochemistry and parasitological culture for the diagnosis of canine leishmaniosis |
| title_full_unstemmed |
Comparative study of in situ hybridization, immunohistochemistry and parasitological culture for the diagnosis of canine leishmaniosis |
| title_sort |
Comparative study of in situ hybridization, immunohistochemistry and parasitological culture for the diagnosis of canine leishmaniosis |
| author |
Furtado, Marina C. |
| author_facet |
Furtado, Marina C. Menezes, Rodrigo C. Kiupel, Matti Madeira, Maria F. Oliveira, Raquel V. C. Langohr, Ingeborg M. Figueiredo, Fabiano B. |
| author_role |
author |
| author2 |
Menezes, Rodrigo C. Kiupel, Matti Madeira, Maria F. Oliveira, Raquel V. C. Langohr, Ingeborg M. Figueiredo, Fabiano B. |
| author2_role |
author author author author author author |
| dc.contributor.author.fl_str_mv |
Furtado, Marina C. Menezes, Rodrigo C. Kiupel, Matti Madeira, Maria F. Oliveira, Raquel V. C. Langohr, Ingeborg M. Figueiredo, Fabiano B. |
| dc.subject.en.pt_BR.fl_str_mv |
Leishmania infantum Dog Diagnosis In situ hybridization Immunohistochemistry Parasitological culture |
| topic |
Leishmania infantum Dog Diagnosis In situ hybridization Immunohistochemistry Parasitological culture |
| description |
Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Pesquisa Clínica em Dermatozoonoses em Animais Domésticos. Rio de Janeiro, RJ, Brasil / Michigan State University. College of Veterinary Medicine. Department of Pathobiology and Diagnostic Investigation. Lansing, MI, USA. |
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2015 |
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2015 |
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2019-02-05T12:48:29Z |
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2019-02-05T12:48:29Z |
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info:eu-repo/semantics/article |
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FURTADO, Marina C. et al. Comparative study of in situ hybridization, immunohistochemistry and parasitological culture for the diagnosis of canine leishmaniosis. Parasites and Vectors, v. 8, n. 620, p. 1-5, 2015. |
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https://www.arca.fiocruz.br/handle/icict/31416 |
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1756-3305 |
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10.1186/s13071-015-1224-4 |
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1756-3305 |
| identifier_str_mv |
FURTADO, Marina C. et al. Comparative study of in situ hybridization, immunohistochemistry and parasitological culture for the diagnosis of canine leishmaniosis. Parasites and Vectors, v. 8, n. 620, p. 1-5, 2015. 1756-3305 10.1186/s13071-015-1224-4 |
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