Polymerase chain reaction (PCR) is highly sensitive for diagnosis of mucosal leishmaniasis.

Detalhes bibliográficos
Autor(a) principal: Oliveira, Jene Greyce Souza de
Data de Publicação: 2005
Outros Autores: Novais, Fernanda Oliveira, Oliveira, Camila Indiani de, Cruz Junior, Antonio Clementino da, Campos, Léon Fábio, Rocha, Any V. da, Boaventura, Viviane Sampaio, Noronha, Almério Libório Lopes de, Costa, Jackson Mauricio Lopes, Barral, Aldina Maria Prado
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/9172
Resumo: Fundação Hospital do Acre. Rio Branco, AC, Brasil
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spelling Oliveira, Jene Greyce Souza deNovais, Fernanda OliveiraOliveira, Camila Indiani deCruz Junior, Antonio Clementino daCampos, Léon FábioRocha, Any V. daBoaventura, Viviane SampaioNoronha, Almério Libório Lopes deCosta, Jackson Mauricio LopesBarral, Aldina Maria Prado2014-12-12T19:19:27Z2014-12-12T19:19:27Z2005OLIVEIRA, J. G. S. et al. Polymerase chain reaction (PCR) is highly sensitive for diagnosis of mucosal leishmaniasis. Acta Tropica, v. 94, n. 1, p. 55-59, 2005.0001-706Xhttps://www.arca.fiocruz.br/handle/icict/917210.1016/j.actatropica.2004.12.003engElsevierPolymerase chain reaction (PCR) is highly sensitive for diagnosis of mucosal leishmaniasis.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Hospital do Acre. Rio Branco, AC, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFundação Hospital do Acre. Rio Branco, AC, BrasilFundação Hospital do Acre. Rio Branco, AC, BrasilFundação Hospital do Acre. Rio Branco, AC, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Faculdade de Medicina da Bahia. Salvador, BA, BrasilWe evaluated the use of polymerase chain reaction (PCR) for diagnosis of mucosal leishmaniasis (ML) in an endemic area in Acre, Brazil, where Leishmania braziliensis is present. Leishmania DNA was detected 34 of 35 cases, yielding a positivity rate of 97.1%, which was higher than the positivity rates for all of the other diagnostic methods studied, namely Montenegro skin test (MST), anti-Leishmania serological testing and microscopic examination of lesion biopsy specimens. These findings have led us to propose guidelines for the diagnosis of ML that use PCR as the principal method of parasitological confirmation of cases.Polymerase chain reactionLeishmania braziliensisMucosal leishmaniasisLeishmania braziliensis/genéticaLeishmaniose Cutânea/diagnósticoAdolescenteAdultoIdosoIdoso de 80 Anos ou maisAnimaisAnticorpos Antiprotozoários/sangueAntiprotozoários/uso terapêuticoBiópsiaBrasilCriançaDNA de Protozoário/químicaFemininoTécnica Indireta de Fluorescência para AnticorpoHumanosLeishmaniose Cutânea/quimioterapiaMasculinoMeglumina/uso terapêuticoMeia-IdadeCompostos Organometálicos/uso terapêuticoSensibilidade e EspecificidadeTestes cutâneosinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://www.arca.fiocruz.br/bitstream/icict/9172/1/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD51ORIGINALOliveira JGS Polymerase chain reaction............pdfOliveira JGS Polymerase chain reaction............pdfapplication/pdf79129https://www.arca.fiocruz.br/bitstream/icict/9172/2/Oliveira%20JGS%20%20Polymerase%20chain%20reaction............pdf1a29eb59800e737e6acc0ee8ecc8c9b7MD52TEXTOliveira JGS Polymerase chain reaction............pdf.txtOliveira JGS Polymerase chain reaction............pdf.txtExtracted texttext/plain21130https://www.arca.fiocruz.br/bitstream/icict/9172/3/Oliveira%20JGS%20%20Polymerase%20chain%20reaction............pdf.txt93b0d3c4b6a6699b4e0af1b3043c5120MD53icict/91722023-03-15 14:33:04.676oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-15T17:33:04Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Polymerase chain reaction (PCR) is highly sensitive for diagnosis of mucosal leishmaniasis.
title Polymerase chain reaction (PCR) is highly sensitive for diagnosis of mucosal leishmaniasis.
spellingShingle Polymerase chain reaction (PCR) is highly sensitive for diagnosis of mucosal leishmaniasis.
Oliveira, Jene Greyce Souza de
Polymerase chain reaction
Leishmania braziliensis
Mucosal leishmaniasis
Leishmania braziliensis/genética
Leishmaniose Cutânea/diagnóstico
Adolescente
Adulto
Idoso
Idoso de 80 Anos ou mais
Animais
Anticorpos Antiprotozoários/sangue
Antiprotozoários/uso terapêutico
Biópsia
Brasil
Criança
DNA de Protozoário/química
Feminino
Técnica Indireta de Fluorescência para Anticorpo
Humanos
Leishmaniose Cutânea/quimioterapia
Masculino
Meglumina/uso terapêutico
Meia-Idade
Compostos Organometálicos/uso terapêutico
Sensibilidade e Especificidade
Testes cutâneos
title_short Polymerase chain reaction (PCR) is highly sensitive for diagnosis of mucosal leishmaniasis.
title_full Polymerase chain reaction (PCR) is highly sensitive for diagnosis of mucosal leishmaniasis.
title_fullStr Polymerase chain reaction (PCR) is highly sensitive for diagnosis of mucosal leishmaniasis.
title_full_unstemmed Polymerase chain reaction (PCR) is highly sensitive for diagnosis of mucosal leishmaniasis.
title_sort Polymerase chain reaction (PCR) is highly sensitive for diagnosis of mucosal leishmaniasis.
author Oliveira, Jene Greyce Souza de
author_facet Oliveira, Jene Greyce Souza de
Novais, Fernanda Oliveira
Oliveira, Camila Indiani de
Cruz Junior, Antonio Clementino da
Campos, Léon Fábio
Rocha, Any V. da
Boaventura, Viviane Sampaio
Noronha, Almério Libório Lopes de
Costa, Jackson Mauricio Lopes
Barral, Aldina Maria Prado
author_role author
author2 Novais, Fernanda Oliveira
Oliveira, Camila Indiani de
Cruz Junior, Antonio Clementino da
Campos, Léon Fábio
Rocha, Any V. da
Boaventura, Viviane Sampaio
Noronha, Almério Libório Lopes de
Costa, Jackson Mauricio Lopes
Barral, Aldina Maria Prado
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Oliveira, Jene Greyce Souza de
Novais, Fernanda Oliveira
Oliveira, Camila Indiani de
Cruz Junior, Antonio Clementino da
Campos, Léon Fábio
Rocha, Any V. da
Boaventura, Viviane Sampaio
Noronha, Almério Libório Lopes de
Costa, Jackson Mauricio Lopes
Barral, Aldina Maria Prado
dc.subject.en.pt_BR.fl_str_mv Polymerase chain reaction
Leishmania braziliensis
Mucosal leishmaniasis
topic Polymerase chain reaction
Leishmania braziliensis
Mucosal leishmaniasis
Leishmania braziliensis/genética
Leishmaniose Cutânea/diagnóstico
Adolescente
Adulto
Idoso
Idoso de 80 Anos ou mais
Animais
Anticorpos Antiprotozoários/sangue
Antiprotozoários/uso terapêutico
Biópsia
Brasil
Criança
DNA de Protozoário/química
Feminino
Técnica Indireta de Fluorescência para Anticorpo
Humanos
Leishmaniose Cutânea/quimioterapia
Masculino
Meglumina/uso terapêutico
Meia-Idade
Compostos Organometálicos/uso terapêutico
Sensibilidade e Especificidade
Testes cutâneos
dc.subject.decs.pt_BR.fl_str_mv Leishmania braziliensis/genética
Leishmaniose Cutânea/diagnóstico
Adolescente
Adulto
Idoso
Idoso de 80 Anos ou mais
Animais
Anticorpos Antiprotozoários/sangue
Antiprotozoários/uso terapêutico
Biópsia
Brasil
Criança
DNA de Protozoário/química
Feminino
Técnica Indireta de Fluorescência para Anticorpo
Humanos
Leishmaniose Cutânea/quimioterapia
Masculino
Meglumina/uso terapêutico
Meia-Idade
Compostos Organometálicos/uso terapêutico
Sensibilidade e Especificidade
Testes cutâneos
description Fundação Hospital do Acre. Rio Branco, AC, Brasil
publishDate 2005
dc.date.issued.fl_str_mv 2005
dc.date.accessioned.fl_str_mv 2014-12-12T19:19:27Z
dc.date.available.fl_str_mv 2014-12-12T19:19:27Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv OLIVEIRA, J. G. S. et al. Polymerase chain reaction (PCR) is highly sensitive for diagnosis of mucosal leishmaniasis. Acta Tropica, v. 94, n. 1, p. 55-59, 2005.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/9172
dc.identifier.issn.none.fl_str_mv 0001-706X
dc.identifier.doi.none.fl_str_mv 10.1016/j.actatropica.2004.12.003
identifier_str_mv OLIVEIRA, J. G. S. et al. Polymerase chain reaction (PCR) is highly sensitive for diagnosis of mucosal leishmaniasis. Acta Tropica, v. 94, n. 1, p. 55-59, 2005.
0001-706X
10.1016/j.actatropica.2004.12.003
url https://www.arca.fiocruz.br/handle/icict/9172
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
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