Thermosensation and the TRPV channel in Rhodnius prolixus.

Detalhes bibliográficos
Autor(a) principal: Zermoglio, Paula F.
Data de Publicação: 2015
Outros Autores: Latorre-Estivalis, José Manuel, Crespo, José E., Lorenzo, Marcelo Gustavo, Lazzari, Claudio R.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/15741
Resumo: 2021-01-01
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spelling Zermoglio, Paula F.Latorre-Estivalis, José ManuelCrespo, José E.Lorenzo, Marcelo GustavoLazzari, Claudio R.2016-09-09T13:42:09Z2016-09-09T13:42:09Z2015ZERMOGLIO, Paula F. et al. Thermosensation and the TRPV channel in Rhodnius prolixus. J Insect Physiol., vol. 81, p. 145-56, 2015.1520-6327https://www.arca.fiocruz.br/handle/icict/1574110.1016/j.jinsphys.2015.07.014engElsevierThermosensation and the TRPV channel in Rhodnius prolixus.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article2021-01-01Université François Rabelais de Tours. Institut de Recherche sur la Biologie de l’Insecte France/Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto IEGEBA. Departamento de Ecología, Genética y Evolución. ArgentinaFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, BrasilUniversité François Rabelais de Tours. Institut de Recherche sur la Biologie de l’Insecte France/Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto IEGEBA. Departamento de Ecología, Genética y Evolución. ArgentinaFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, BrasilUniversité François Rabelais de Tours. Institut de Recherche sur la Biologie de l’Insecte FranceThe thermal sense of triatomine bugs, vectors of Chagas disease, is unique among insects. Not only do these bugs exhibit the highest sensitivity to heat known in any animal up to date, but they can also perceive the infrared radiation emitted by the body of their warm-blooded hosts. The sensory basis of this capacity has just started to be unravelled. To shed additional light on our understanding of thermosensation, we initiated an analysis of the genetic basis of the thermal sense in Rhodnius prolixus. We tested the hypothesis that a TRPV (transient receptor potential vanilloid) channel receptor is involved in the evaluation of heat in this species. Two different approaches were adopted. Initially, we analysed the expression of a TRPV candidate for this function, i.e., RproIav, in different tissues. Subsequently, we tested the effects of capsaicin and capsazepine, two molecules known to interact with mammal TRPV1, using three different behavioural protocols for evaluating thermal responses: (1) proboscis extension response (PER), (2) thermopreference in a temperature gradient and (3) spatial learning in an operant conditioning context. Bioinformatic analyses confirmed that the characteristic features typical of the TRPV channel subfamily are found in the RproIav protein sequence. Molecular analysis showed that RproIav is expressed in R. prolixus, not only in the antennae, but also in other body structures bearing sensory organs. Behavioural experiments consistently revealed that capsaicin treated insects are less responsive to heat stimuli and prefer lower temperatures than non-treated insects, and that they fail to orient in space. Conversely, capsazepine induces the opposite behaviours. The latter data suggest that triatomine thermoreception is based on the activation of a TRP channel, with a similar mechanism to that described for mammal TRPV1. The expression of RproIav in diverse sensory structures suggests that this receptor channel is potentially involved in bug thermoreception. This constitutes solid evidence that thermosensation could be based on the activation of TRP receptors that are expressed in different tissues in R. prolixus. Whether RproIav channel is a potential target for the compounds tested and whether it mediates the observed effects on behaviour still deserves to be confirmed by further research.CapsaicinCapsazepineChagas disease vectorsTRPVThermal senseinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/15741/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALve_Paula_Zermoglio_Thermosensation_CPqRR_2015.pdfve_Paula_Zermoglio_Thermosensation_CPqRR_2015.pdfapplication/pdf8900087https://www.arca.fiocruz.br/bitstream/icict/15741/2/ve_Paula_Zermoglio_Thermosensation_CPqRR_2015.pdf54a9a39b670bbcb14c339956945bfe22MD52TEXTve_Paula_Zermoglio_Thermosensation_CPqRR_2015.pdf.txtve_Paula_Zermoglio_Thermosensation_CPqRR_2015.pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv Thermosensation and the TRPV channel in Rhodnius prolixus.
title Thermosensation and the TRPV channel in Rhodnius prolixus.
spellingShingle Thermosensation and the TRPV channel in Rhodnius prolixus.
Zermoglio, Paula F.
Capsaicin
Capsazepine
Chagas disease vectors
TRPV
Thermal sense
title_short Thermosensation and the TRPV channel in Rhodnius prolixus.
title_full Thermosensation and the TRPV channel in Rhodnius prolixus.
title_fullStr Thermosensation and the TRPV channel in Rhodnius prolixus.
title_full_unstemmed Thermosensation and the TRPV channel in Rhodnius prolixus.
title_sort Thermosensation and the TRPV channel in Rhodnius prolixus.
author Zermoglio, Paula F.
author_facet Zermoglio, Paula F.
Latorre-Estivalis, José Manuel
Crespo, José E.
Lorenzo, Marcelo Gustavo
Lazzari, Claudio R.
author_role author
author2 Latorre-Estivalis, José Manuel
Crespo, José E.
Lorenzo, Marcelo Gustavo
Lazzari, Claudio R.
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Zermoglio, Paula F.
Latorre-Estivalis, José Manuel
Crespo, José E.
Lorenzo, Marcelo Gustavo
Lazzari, Claudio R.
dc.subject.en.pt_BR.fl_str_mv Capsaicin
Capsazepine
Chagas disease vectors
TRPV
Thermal sense
topic Capsaicin
Capsazepine
Chagas disease vectors
TRPV
Thermal sense
description 2021-01-01
publishDate 2015
dc.date.issued.fl_str_mv 2015
dc.date.accessioned.fl_str_mv 2016-09-09T13:42:09Z
dc.date.available.fl_str_mv 2016-09-09T13:42:09Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv ZERMOGLIO, Paula F. et al. Thermosensation and the TRPV channel in Rhodnius prolixus. J Insect Physiol., vol. 81, p. 145-56, 2015.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/15741
dc.identifier.issn.pt_BR.fl_str_mv 1520-6327
dc.identifier.doi.none.fl_str_mv 10.1016/j.jinsphys.2015.07.014
identifier_str_mv ZERMOGLIO, Paula F. et al. Thermosensation and the TRPV channel in Rhodnius prolixus. J Insect Physiol., vol. 81, p. 145-56, 2015.
1520-6327
10.1016/j.jinsphys.2015.07.014
url https://www.arca.fiocruz.br/handle/icict/15741
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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https://www.arca.fiocruz.br/bitstream/icict/15741/2/ve_Paula_Zermoglio_Thermosensation_CPqRR_2015.pdf
https://www.arca.fiocruz.br/bitstream/icict/15741/3/ve_Paula_Zermoglio_Thermosensation_CPqRR_2015.pdf.txt
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