Next generation of ovarian cancer detection using aptamers

Abreu, Rayane da Silva; Antunes, Deborah; Moreira, Aline dos Santos; Passetti, Fabio; Mendonça, Julia Badaró; Araújo, Natássia Silva de; Sassaro, Tayanne Felippe; Alberto, Anael Viana Pinto; Carrossini, Nina; Fernandes, Priscila Valverde; Costa, Mayla Abrahim; Guimarães, Ana Carolina Ramos; Degrave, Wim Maurits Sylvain; Waghabi, Mariana Caldas

Next generation of ovarian cancer detection using aptamers

Detalhes bibliográficos
Autor(a) principal:	Abreu, Rayane da Silva
Data de Publicação:	2023
Outros Autores:	Antunes, Deborah, Moreira, Aline dos Santos, Passetti, Fabio, Mendonça, Julia Badaró, Araújo, Natássia Silva de, Sassaro, Tayanne Felippe, Alberto, Anael Viana Pinto, Carrossini, Nina, Fernandes, Priscila Valverde, Costa, Mayla Abrahim, Guimarães, Ana Carolina Ramos, Degrave, Wim Maurits Sylvain, Waghabi, Mariana Caldas
Tipo de documento:	Artigo
Idioma:	por
Título da fonte:	Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo:	https://www.arca.fiocruz.br/handle/icict/58543 https:// doi.org/10.3390/ijms24076315
Resumo:	Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Genômica Funcional e Bioinformática. Rio de Janeiro, RJ, Brasil.

Metadados do item

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network_acronym_str	CRUZ
network_name_str	Repositório Institucional da FIOCRUZ (ARCA)
repository_id_str	2135
spelling	Abreu, Rayane da SilvaAntunes, DeborahMoreira, Aline dos SantosPassetti, FabioMendonça, Julia BadaróAraújo, Natássia Silva deSassaro, Tayanne FelippeAlberto, Anael Viana PintoCarrossini, NinaFernandes, Priscila ValverdeCosta, Mayla AbrahimGuimarães, Ana Carolina RamosDegrave, Wim Maurits SylvainWaghabi, Mariana Caldas2023-05-22T12:42:33Z2023-05-22T12:42:33Z2023ABREU, Rayane da Silva et al. Next generation of ovarian cancer detection using aptamers. Int. J. Mol. Sci, v. 24, n. 6315, p. 1-28, 2023.1422-0067https://www.arca.fiocruz.br/handle/icict/58543https:// doi.org/10.3390/ijms24076315porMDPINext generation of ovarian cancer detection using aptamersinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Genômica Funcional e Bioinformática. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Genômica Funcional e Bioinformática. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Genômica Funcional e Bioinformática. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Genômica Funcional e Bioinformática. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Genômica Funcional e Bioinformática. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Genômica Funcional e Bioinformática. Rio de Janeiro, RJ, Brasil.Instituto Nacional de Tecnologia. Laboratório de Tecnologia de Pós. Rio de Janeiro, RJ, Brasil.Instituto Nacional do Câncer. Divisão de Patologia. Rio de Janeiro, RJ, Brasil.Instituto Nacional do Câncer. Divisão de Patologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos. Laboratório de Tecnologia Imunológica. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Genômica Funcional e Bioinformática. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Genômica Funcional e Bioinformática. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Genômica Funcional e Bioinformática. Rio de Janeiro, RJ, Brasil.Ovarian cancer is among the seven most common types of cancer in women, being the most fatal gynecological tumor, due to the difficulty of detection in early stages. Aptamers are important tools to improve tumor diagnosis through the recognition of specific molecules produced by tumors. Here, aptamers and their potential targets in ovarian cancer cells were analyzed by in silico approaches. Specific aptamers were selected by the Cell-SELEX method using Caov-3 and OvCar-3 cells. The five most frequent aptamers obtained from the last round of selection were computationally modeled. The potential targets for those aptamers in cells were proposed by analyzing proteomic data available for the Caov-3 and OvCar-3 cell lines. Overexpressed proteins for each cell were characterized as to their three-dimensional model, cell location, and electrostatic potential. As a result, four specific aptamers for ovarian tumors were selected: AptaC2, AptaC4, AptaO1, and AptaO2. Potential targets were identified for each aptamer through Molecular Docking, and the best complexes were AptaC2-FXYD3, AptaC4-ALPP, AptaO1-TSPAN15, and AptaO2-TSPAN15. In addition, AptaC2 and AptaO1 could detect different stages and subtypes of ovarian cancer tissue samples. The application of this technology makes it possible to propose new molecular biomarkers for the differential diagnosis of epithelial ovarian cancer.Carcinoma, Ovarian EpithelialAptamersComputer SimulationCarcinoma Epitelial de OvarioAptámerosSimulación por ComputadorCarcinome épithélial de l'ovaireAptamèresSimulation numériqueCarcinoma Epitelial do OvárioAptâmerosModelagem Computacionalinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/58543/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALijms-24-06315OK.pdfijms-24-06315OK.pdfapplication/pdf5645964https://www.arca.fiocruz.br/bitstream/icict/58543/2/ijms-24-06315OK.pdf4237cb9a39c847a1ee20d085318206ddMD52icict/585432023-05-22 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dc.title.en_US.fl_str_mv	Next generation of ovarian cancer detection using aptamers
title	Next generation of ovarian cancer detection using aptamers
spellingShingle	Next generation of ovarian cancer detection using aptamers Abreu, Rayane da Silva Carcinoma, Ovarian Epithelial Aptamers Computer Simulation Carcinoma Epitelial de Ovario Aptámeros Simulación por Computador Carcinome épithélial de l'ovaire Aptamères Simulation numérique Carcinoma Epitelial do Ovário Aptâmeros Modelagem Computacional
title_short	Next generation of ovarian cancer detection using aptamers
title_full	Next generation of ovarian cancer detection using aptamers
title_fullStr	Next generation of ovarian cancer detection using aptamers
title_full_unstemmed	Next generation of ovarian cancer detection using aptamers
title_sort	Next generation of ovarian cancer detection using aptamers
author	Abreu, Rayane da Silva
author_facet	Abreu, Rayane da Silva Antunes, Deborah Moreira, Aline dos Santos Passetti, Fabio Mendonça, Julia Badaró Araújo, Natássia Silva de Sassaro, Tayanne Felippe Alberto, Anael Viana Pinto Carrossini, Nina Fernandes, Priscila Valverde Costa, Mayla Abrahim Guimarães, Ana Carolina Ramos Degrave, Wim Maurits Sylvain Waghabi, Mariana Caldas
author_role	author
author2	Antunes, Deborah Moreira, Aline dos Santos Passetti, Fabio Mendonça, Julia Badaró Araújo, Natássia Silva de Sassaro, Tayanne Felippe Alberto, Anael Viana Pinto Carrossini, Nina Fernandes, Priscila Valverde Costa, Mayla Abrahim Guimarães, Ana Carolina Ramos Degrave, Wim Maurits Sylvain Waghabi, Mariana Caldas
author2_role	author author author author author author author author author author author author author
dc.contributor.author.fl_str_mv	Abreu, Rayane da Silva Antunes, Deborah Moreira, Aline dos Santos Passetti, Fabio Mendonça, Julia Badaró Araújo, Natássia Silva de Sassaro, Tayanne Felippe Alberto, Anael Viana Pinto Carrossini, Nina Fernandes, Priscila Valverde Costa, Mayla Abrahim Guimarães, Ana Carolina Ramos Degrave, Wim Maurits Sylvain Waghabi, Mariana Caldas
dc.subject.en.en_US.fl_str_mv	Carcinoma, Ovarian Epithelial Aptamers Computer Simulation
topic	Carcinoma, Ovarian Epithelial Aptamers Computer Simulation Carcinoma Epitelial de Ovario Aptámeros Simulación por Computador Carcinome épithélial de l'ovaire Aptamères Simulation numérique Carcinoma Epitelial do Ovário Aptâmeros Modelagem Computacional
dc.subject.es.en_US.fl_str_mv	Carcinoma Epitelial de Ovario Aptámeros Simulación por Computador
dc.subject.fr.en_US.fl_str_mv	Carcinome épithélial de l'ovaire Aptamères Simulation numérique
dc.subject.decs.en_US.fl_str_mv	Carcinoma Epitelial do Ovário Aptâmeros Modelagem Computacional
description	Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Genômica Funcional e Bioinformática. Rio de Janeiro, RJ, Brasil.
publishDate	2023
dc.date.accessioned.fl_str_mv	2023-05-22T12:42:33Z
dc.date.available.fl_str_mv	2023-05-22T12:42:33Z
dc.date.issued.fl_str_mv	2023
dc.type.status.fl_str_mv	info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv	info:eu-repo/semantics/article
format	article
status_str	publishedVersion
dc.identifier.citation.fl_str_mv	ABREU, Rayane da Silva et al. Next generation of ovarian cancer detection using aptamers. Int. J. Mol. Sci, v. 24, n. 6315, p. 1-28, 2023.
dc.identifier.uri.fl_str_mv	https://www.arca.fiocruz.br/handle/icict/58543
dc.identifier.issn.en_US.fl_str_mv	1422-0067
dc.identifier.doi.none.fl_str_mv	https:// doi.org/10.3390/ijms24076315
identifier_str_mv	ABREU, Rayane da Silva et al. Next generation of ovarian cancer detection using aptamers. Int. J. Mol. Sci, v. 24, n. 6315, p. 1-28, 2023. 1422-0067
url	https://www.arca.fiocruz.br/handle/icict/58543 https:// doi.org/10.3390/ijms24076315
dc.language.iso.fl_str_mv	por
language	por
dc.rights.driver.fl_str_mv	info:eu-repo/semantics/openAccess
eu_rights_str_mv	openAccess
dc.publisher.none.fl_str_mv	MDPI
publisher.none.fl_str_mv	MDPI
dc.source.none.fl_str_mv	reponame:Repositório Institucional da FIOCRUZ (ARCA) instname:Fundação Oswaldo Cruz (FIOCRUZ) instacron:FIOCRUZ
instname_str	Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str	FIOCRUZ
institution	FIOCRUZ
reponame_str	Repositório Institucional da FIOCRUZ (ARCA)
collection	Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv	https://www.arca.fiocruz.br/bitstream/icict/58543/1/license.txt https://www.arca.fiocruz.br/bitstream/icict/58543/2/ijms-24-06315OK.pdf
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repository.name.fl_str_mv	Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
repository.mail.fl_str_mv	repositorio.arca@fiocruz.br
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Next generation of ovarian cancer detection using aptamers

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