Validation of quantitative real-time PCR for the in vitro assessment of antileishmanial drug activity

Detalhes bibliográficos
Autor(a) principal: Gomes, Luciana Inácia
Data de Publicação: 2012
Outros Autores: Gonzaga, Felipe M., Teixeira, Eliane de Morais, Rodrigues, Bruna Soares de Souza Lima, Freire, Verônica V., Rabello, Ana Lúcia Teles
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/7458
Resumo: Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratory of Clinical Research. Belo Horizonte, Minas Gerais, Brazil
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spelling Gomes, Luciana InáciaGonzaga, Felipe M.Teixeira, Eliane de MoraisRodrigues, Bruna Soares de Souza LimaFreire, Verônica V.Rabello, Ana Lúcia Teles2014-03-26T18:48:08Z2014-03-26T18:48:08Z2012GOMES, Luciana Inácia et al. Validation of quantitative real-time PCR for the in vitro assessment of antileishmanial drug activity. Experimental Parasitology. 2012, vol.131, pp. 175-1790014-4894https://www.arca.fiocruz.br/handle/icict/745810.1016/j.exppara.2012.03.021engElsevierValidation of quantitative real-time PCR for the in vitro assessment of antileishmanial drug activityinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratory of Clinical Research. Belo Horizonte, Minas Gerais, BrazilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratory of Clinical Research. Belo Horizonte, Minas Gerais, BrazilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratory of Clinical Research. Belo Horizonte, Minas Gerais, BrazilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratory of Clinical Research. Belo Horizonte, Minas Gerais, BrazilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratory of Clinical Research. Belo Horizonte, Minas Gerais, BrazilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratory of Clinical Research. Belo Horizonte, Minas Gerais, BrazilIn vitro assays play an important role in the discovery and development of new antileishmanial drugs. The classic macrophage-amastigote models using murine peritoneal macrophages or human-monocyte derived macrophages as host cells are useful for drug screening. A major limitation of these models is the dependence on microscopic counting, a time-consuming and subjective method of analysis. The present study describes a detailed protocol for applying quantitative real-time PCR (qPCR) as an accurate and sensitive tool to assess parasite load in an amastigote-macrophage model. This assay can be performed in a standardized medium-to-high throughput procedure, replacing traditional readout of number of amastigote per macrophages by DNA load measurement.antileishmanial drugsLeishmaniaIn vitro assaysQuantitative real-time PCRinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALValidation of quantitative real-time PCR for the in vitro assessment of antileishmanial drug activity.pdfValidation of quantitative real-time PCR for the in vitro assessment of antileishmanial drug activity.pdfapplication/pdf1490006https://www.arca.fiocruz.br/bitstream/icict/7458/1/Validation%20of%20quantitative%20real-time%20PCR%20for%20the%20in%20vitro%20assessment%20of%20antileishmanial%20drug%20activity.pdf3b53b9cfc78ee79888715df42d6030d9MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://www.arca.fiocruz.br/bitstream/icict/7458/2/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD52TEXTValidation of quantitative real-time PCR for the in vitro assessment of antileishmanial drug activity.pdf.txtValidation of quantitative real-time PCR for the in vitro assessment of antileishmanial drug activity.pdf.txtExtracted texttext/plain5https://www.arca.fiocruz.br/bitstream/icict/7458/5/Validation%20of%20quantitative%20real-time%20PCR%20for%20the%20in%20vitro%20assessment%20of%20antileishmanial%20drug%20activity.pdf.txtcc9067c2ee470dc248b14b194209a34eMD55THUMBNAILValidation of quantitative real-time PCR for the in vitro assessment of antileishmanial drug activity.pdf.jpgValidation of quantitative real-time PCR for the in vitro assessment of antileishmanial drug activity.pdf.jpgGenerated Thumbnailimage/jpeg1899https://www.arca.fiocruz.br/bitstream/icict/7458/4/Validation%20of%20quantitative%20real-time%20PCR%20for%20the%20in%20vitro%20assessment%20of%20antileishmanial%20drug%20activity.pdf.jpg303a4df57812542555b5aad8e1a5a276MD54icict/74582018-04-06 11:25:54.662oai:www.arca.fiocruz.br:icict/7458TElDRU7Dh0EgREUgRElTVFJJQlVJw4fDg08gTsODTy1FWENMVVNJVkEKCkFvIGNvbmNvcmRhciBlIGFjZWl0YXIgZXN0YSBsaWNlbsOnYSB2b2PDqiAoYXV0b3Igb3UgZGV0ZW50b3IgZG9zIGRpcmVpdG9zIGF1dG9yYWlzKToKCmEpIERlY2xhcmEgcXVlIGNvbmhlY2UgYSBwb2zDrXRpY2EgZGUgY29weXJpZ2h0IGRhIGVkaXRvcmEgZG8gc2V1IGRvY3VtZW50by4KCmIpIERlY2xhcmEgcXVlIGNvbmhlY2UgZSBhY2VpdGEgYXMgRGlyZXRyaXplcyBwYXJhIG8gUmVwb3NpdMOzcmlvIEluc3RpdHVjaW9uYWwgZGEgRnVuZGHDp8OjbyBPc3dhbGRvIENydXogKEZJT0NSVVopLgoKYykgQ29uY2VkZSDDoCBGSU9DUlVaIG8gZGlyZWl0byBuw6NvLWV4Y2x1c2l2byBkZSBhcnF1aXZhciwgcmVwcm9kdXppciwgY29udmVydGVyIChjb21vIGRlZmluaWRvIGEgc2VndWlyKSwgY29tdW5pY2FyCiAKZS9vdSBkaXN0cmlidWlyIG5vIFJlcG9zaXTDs3JpbyBkYSBGSU9DUlVaLCBvIGRvY3VtZW50byBlbnRyZWd1ZSAoaW5jbHVpbmRvIG8gcmVzdW1vL2Fic3RyYWN0KSBlbSBmb3JtYXRvIGRpZ2l0YWwgb3UgCgpwb3IgcXVhbHF1ZXIgb3V0cm8gbWVpby4KCmQpIERlY2xhcmEgcXVlIGF1dG9yaXphIGEgRklPQ1JVWiBhIGFycXVpdmFyIG1haXMgZGUgdW1hIGPDs3BpYSBkZXN0ZSBkb2N1bWVudG8gZSBjb252ZXJ0w6otbG8sIHNlbSBhbHRlcmFyIG8gc2V1IGNvbnRlw7pkbywgCgpwYXJhIHF1YWxxdWVyIGZvcm1hdG8gZGUgYXJxdWl2bywgbWVpbyBvdSBzdXBvcnRlLCBwYXJhIGVmZWl0b3MgZGUgc2VndXJhbsOnYSwgcHJlc2VydmHDp8OjbyAoYmFja3VwKSBlIGFjZXNzby4KCmUpIERlY2xhcmEgcXVlIG8gZG9jdW1lbnRvIHN1Ym1ldGlkbyDDqSBvIHNldSB0cmFiYWxobyBvcmlnaW5hbCwgZSBxdWUgZGV0w6ltIG8gZGlyZWl0byBkZSBjb25jZWRlciBhIHRlcmNlaXJvcyBvcyBkaXJlaXRvcyAKCmNvbnRpZG9zIG5lc3RhIGxpY2Vuw6dhLiBEZWNsYXJhIHRhbWLDqW0gcXVlIGEgZW50cmVnYSBkbyBkb2N1bWVudG8gbsOjbyBpbmZyaW5nZSBvcyBkaXJlaXRvcyBkZSBxdWFscXVlciBvdXRyYSBwZXNzb2Egb3UgZW50aWRhZGUuCgpmKSBEZWNsYXJhIHF1ZSwgbm8gY2FzbyBkbyBkb2N1bWVudG8gc3VibWV0aWRvIGNvbnRlciBtYXRlcmlhbCBkbyBxdWFsIG7Do28gZGV0w6ltIG9zIGRpcmVpdG9zIGRlIGF1dG9yLCBvYnRldmUgYSBhdXRvcml6YcOnw6NvIAoKaXJyZXN0cml0YSBkbyByZXNwZWN0aXZvIGRldGVudG9yIGRlc3NlcyBkaXJlaXRvcywgcGFyYSBjZWRlciBhIEZJT0NSVVogb3MgZGlyZWl0b3MgcmVxdWVyaWRvcyBwb3IgZXN0YSBMaWNlbsOnYSBlIGF1dG9yaXphciBhIAoKdXRpbGl6w6EtbG9zIGxlZ2FsbWVudGUuIERlY2xhcmEgdGFtYsOpbSBxdWUgZXNzZSBtYXRlcmlhbCBjdWpvcyBkaXJlaXRvcyBzw6NvIGRlIHRlcmNlaXJvcyBlc3TDoSBjbGFyYW1lbnRlIGlkZW50aWZpY2FkbyBlIHJlY29uaGVjaWRvIAoKbm8gdGV4dG8gb3UgY29udGXDumRvIGRvIGRvY3VtZW50byBlbnRyZWd1ZS4KCmcpIFNFIE8gRE9DVU1FTlRPIEVOVFJFR1VFIMOJIEJBU0VBRE8gRU0gVFJBQkFMSE8gRklOQU5DSUFETyBPVSBBUE9JQURPIFBPUiBPVVRSQSBJTlNUSVRVScOHw4NPIFFVRSBOw4NPIEEgRklPQ1JVWiwgREVDTEFSQSBRVUUgQ1VNUFJJVSAKClFVQUlTUVVFUiBPQlJJR0HDh8OVRVMgRVhJR0lEQVMgUEVMTyBSRVNQRUNUSVZPIENPTlRSQVRPIE9VIEFDT1JETy4gQSBGSU9DUlVaIGlkZW50aWZpY2Fyw6EgY2xhcmFtZW50ZSBvKHMpIG5vbWUocykgZG8ocykgYXV0b3IoZXMpIGRvcyAKCmRpcmVpdG9zIGRvIGRvY3VtZW50byBlbnRyZWd1ZSBlIG7Do28gZmFyw6EgcXVhbHF1ZXIgYWx0ZXJhw6fDo28sIHBhcmEgYWzDqW0gZG8gcHJldmlzdG8gbmEgYWzDrW5lYSBjKS4KRepositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352018-04-06T14:25:54Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Validation of quantitative real-time PCR for the in vitro assessment of antileishmanial drug activity
title Validation of quantitative real-time PCR for the in vitro assessment of antileishmanial drug activity
spellingShingle Validation of quantitative real-time PCR for the in vitro assessment of antileishmanial drug activity
Gomes, Luciana Inácia
antileishmanial drugs
Leishmania
In vitro assays
Quantitative real-time PCR
title_short Validation of quantitative real-time PCR for the in vitro assessment of antileishmanial drug activity
title_full Validation of quantitative real-time PCR for the in vitro assessment of antileishmanial drug activity
title_fullStr Validation of quantitative real-time PCR for the in vitro assessment of antileishmanial drug activity
title_full_unstemmed Validation of quantitative real-time PCR for the in vitro assessment of antileishmanial drug activity
title_sort Validation of quantitative real-time PCR for the in vitro assessment of antileishmanial drug activity
author Gomes, Luciana Inácia
author_facet Gomes, Luciana Inácia
Gonzaga, Felipe M.
Teixeira, Eliane de Morais
Rodrigues, Bruna Soares de Souza Lima
Freire, Verônica V.
Rabello, Ana Lúcia Teles
author_role author
author2 Gonzaga, Felipe M.
Teixeira, Eliane de Morais
Rodrigues, Bruna Soares de Souza Lima
Freire, Verônica V.
Rabello, Ana Lúcia Teles
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Gomes, Luciana Inácia
Gonzaga, Felipe M.
Teixeira, Eliane de Morais
Rodrigues, Bruna Soares de Souza Lima
Freire, Verônica V.
Rabello, Ana Lúcia Teles
dc.subject.en.pt_BR.fl_str_mv antileishmanial drugs
Leishmania
In vitro assays
Quantitative real-time PCR
topic antileishmanial drugs
Leishmania
In vitro assays
Quantitative real-time PCR
description Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratory of Clinical Research. Belo Horizonte, Minas Gerais, Brazil
publishDate 2012
dc.date.issued.fl_str_mv 2012
dc.date.accessioned.fl_str_mv 2014-03-26T18:48:08Z
dc.date.available.fl_str_mv 2014-03-26T18:48:08Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv GOMES, Luciana Inácia et al. Validation of quantitative real-time PCR for the in vitro assessment of antileishmanial drug activity. Experimental Parasitology. 2012, vol.131, pp. 175-179
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/7458
dc.identifier.issn.none.fl_str_mv 0014-4894
dc.identifier.doi.none.fl_str_mv 10.1016/j.exppara.2012.03.021
identifier_str_mv GOMES, Luciana Inácia et al. Validation of quantitative real-time PCR for the in vitro assessment of antileishmanial drug activity. Experimental Parasitology. 2012, vol.131, pp. 175-179
0014-4894
10.1016/j.exppara.2012.03.021
url https://www.arca.fiocruz.br/handle/icict/7458
dc.language.iso.fl_str_mv eng
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dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
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