Detection of Wuchereria bancrofti DNA in paired serum and urine samples using polymerase chain reaction-based systems
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2014 |
| Outros Autores: | , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/23200 |
Resumo: | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil. |
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Ximenes, CamilaBrandão, EduardoOliveira, PaulaRocha, AbrahamRego, TamisaMedeiros, RafaelAguiar-Santos, Ana MariaFerraz, JoãoReis, ChristianAraujo, PauloCarvalho, LuizMelo, Fabio L.2017-11-16T12:16:25Z2017-11-16T12:16:25Z2014XIMENES, C. et al. Detection of Wuchereria bancrofti DNA in paired serum and urine samples using polymerase chain reaction-based systems. Memorias Do Instituto Oswaldo Cruz, v. 109, n. 8, p. 978–983, dez. 2014.1678-8060https://www.arca.fiocruz.br/handle/icict/2320010.1590/0074-0276140155engDNA, Helminthisolation & purificationElephantiasis, FilarialdiagnosisMicrofilariaeisolation & purificationWuchereria bancroftiisolation & purificationAnimalsYoung AdultLimit of DetectionHumansSensitivity and SpecificityAdolescentWuchereria bancroftiDNADiagnósticoFilariosePCRurinaWuchereria bancroftiDNAdiagnosisfilariasisPCRurineDetection of Wuchereria bancrofti DNA in paired serum and urine samples using polymerase chain reaction-based systemsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil.The Global Program for the Elimination of Lymphatic Filariasis (GPELF) aims to eliminate this disease by the year 2020. However, the development of more specific and sensitive tests is important for the success of the GPELF. The present study aimed to standardise polymerase chain reaction (PCR)-based systems for the diagnosis of filariasis in serum and urine. Twenty paired biological urine and serum samples from individuals already known to be positive for Wuchereria bancrofti were collected during the day. Conventional PCR and semi-nested PCR assays were optimised. The detection limit of the technique for purified W. bancrofti DNA extracted from adult worms was 10 fg for the internal systems (WbF/Wb2) and 0.1 fg by using semi-nested PCR. The specificity of the primers was confirmed experimentally by amplification of 1 ng of purified genomic DNA from other species of parasites. Evaluation of the paired urine and serum samples by the semi-nested PCR technique indicated only two of the 20 tested individuals were positive, whereas the simple internal PCR system (WbF/Wb2), which has highly promising performance, revealed that all the patients were positive using both samples. This study successfully demonstrated the possibility of using the PCR technique on urine for the diagnosis of W. bancrofti infection.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83092https://www.arca.fiocruz.br/bitstream/icict/23200/1/license.txt447f2497af1ef5cf99b5c07f6fa18dceMD51ORIGINALart. Detection of Wuchereria - ximenes.pdfart. Detection of Wuchereria - ximenes.pdfapplication/pdf857454https://www.arca.fiocruz.br/bitstream/icict/23200/2/art.%20Detection%20of%20Wuchereria%20-%20ximenes.pdfd436637305b8bb9690f436b2a1dfcfbcMD52TEXTart. Detection of Wuchereria - ximenes.pdf.txtart. 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| dc.title.pt_BR.fl_str_mv |
Detection of Wuchereria bancrofti DNA in paired serum and urine samples using polymerase chain reaction-based systems |
| title |
Detection of Wuchereria bancrofti DNA in paired serum and urine samples using polymerase chain reaction-based systems |
| spellingShingle |
Detection of Wuchereria bancrofti DNA in paired serum and urine samples using polymerase chain reaction-based systems Ximenes, Camila DNA, Helminth isolation & purification Elephantiasis, Filarial diagnosis Microfilariae isolation & purification Wuchereria bancrofti isolation & purification Animals Young Adult Limit of Detection Humans Sensitivity and Specificity Adolescent Wuchereria bancrofti DNA Diagnóstico Filariose PCR urina Wuchereria bancrofti DNA diagnosis filariasis PCR urine |
| title_short |
Detection of Wuchereria bancrofti DNA in paired serum and urine samples using polymerase chain reaction-based systems |
| title_full |
Detection of Wuchereria bancrofti DNA in paired serum and urine samples using polymerase chain reaction-based systems |
| title_fullStr |
Detection of Wuchereria bancrofti DNA in paired serum and urine samples using polymerase chain reaction-based systems |
| title_full_unstemmed |
Detection of Wuchereria bancrofti DNA in paired serum and urine samples using polymerase chain reaction-based systems |
| title_sort |
Detection of Wuchereria bancrofti DNA in paired serum and urine samples using polymerase chain reaction-based systems |
| author |
Ximenes, Camila |
| author_facet |
Ximenes, Camila Brandão, Eduardo Oliveira, Paula Rocha, Abraham Rego, Tamisa Medeiros, Rafael Aguiar-Santos, Ana Maria Ferraz, João Reis, Christian Araujo, Paulo Carvalho, Luiz Melo, Fabio L. |
| author_role |
author |
| author2 |
Brandão, Eduardo Oliveira, Paula Rocha, Abraham Rego, Tamisa Medeiros, Rafael Aguiar-Santos, Ana Maria Ferraz, João Reis, Christian Araujo, Paulo Carvalho, Luiz Melo, Fabio L. |
| author2_role |
author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Ximenes, Camila Brandão, Eduardo Oliveira, Paula Rocha, Abraham Rego, Tamisa Medeiros, Rafael Aguiar-Santos, Ana Maria Ferraz, João Reis, Christian Araujo, Paulo Carvalho, Luiz Melo, Fabio L. |
| dc.subject.mesh.pt_BR.fl_str_mv |
DNA, Helminth isolation & purification Elephantiasis, Filarial diagnosis Microfilariae isolation & purification Wuchereria bancrofti isolation & purification Animals Young Adult Limit of Detection Humans Sensitivity and Specificity Adolescent |
| topic |
DNA, Helminth isolation & purification Elephantiasis, Filarial diagnosis Microfilariae isolation & purification Wuchereria bancrofti isolation & purification Animals Young Adult Limit of Detection Humans Sensitivity and Specificity Adolescent Wuchereria bancrofti DNA Diagnóstico Filariose PCR urina Wuchereria bancrofti DNA diagnosis filariasis PCR urine |
| dc.subject.other.pt_BR.fl_str_mv |
Wuchereria bancrofti DNA Diagnóstico Filariose PCR urina |
| dc.subject.en.pt_BR.fl_str_mv |
Wuchereria bancrofti DNA diagnosis filariasis PCR urine |
| description |
Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil. |
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2014 |
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2014 |
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2017-11-16T12:16:25Z |
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2017-11-16T12:16:25Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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XIMENES, C. et al. Detection of Wuchereria bancrofti DNA in paired serum and urine samples using polymerase chain reaction-based systems. Memorias Do Instituto Oswaldo Cruz, v. 109, n. 8, p. 978–983, dez. 2014. |
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https://www.arca.fiocruz.br/handle/icict/23200 |
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1678-8060 |
| dc.identifier.doi.none.fl_str_mv |
10.1590/0074-0276140155 |
| identifier_str_mv |
XIMENES, C. et al. Detection of Wuchereria bancrofti DNA in paired serum and urine samples using polymerase chain reaction-based systems. Memorias Do Instituto Oswaldo Cruz, v. 109, n. 8, p. 978–983, dez. 2014. 1678-8060 10.1590/0074-0276140155 |
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