Identification of Immunogenic Linear B-Cell Epitopes in C. burnetii Outer Membrane Proteins Using Immunoinformatics Approaches Reveals Potential Targets of Persistent Infections

Detalhes bibliográficos
Autor(a) principal: Fontes, Sílvia da Silva
Data de Publicação: 2021
Outros Autores: Maia, Fernanda de Moraes, Ataides, Laura Santa’Anna, Conte, Fernando Paiva, Lima Junior, Josué da Costa, Rozental, Tatiana, Assis, Matheus Ribeiro da Silva, Pessoa Júnior, Adonai Alvino, Fernandes, Jorlan, Lemos, Elba Regina Sampaio de, Silva, Rodrigo Nunes Rodrigues da
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
DOI: 10.3390/pathogens10101250
Texto Completo: https://www.arca.fiocruz.br/handle/icict/50919
Resumo: Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos. Laboratório de Tecnologia de Anticorpos Monoclonais. Rio de Janeiro, RJ, Brasil.
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spelling Fontes, Sílvia da SilvaMaia, Fernanda de MoraesAtaides, Laura Santa’AnnaConte, Fernando PaivaLima Junior, Josué da CostaRozental, TatianaAssis, Matheus Ribeiro da SilvaPessoa Júnior, Adonai AlvinoFernandes, JorlanLemos, Elba Regina Sampaio deSilva, Rodrigo Nunes Rodrigues da2022-01-27T17:54:48Z2022-01-27T17:54:48Z2021FONTES, Sílvia da Silva et al. Identification of Immunogenic Linear B-Cell Epitopes in C. burnetii Outer Membrane Proteins Using Immunoinformatics Approaches Reveals Potential Targets of Persistent Infections. Pathogens, v. 10, 1250, p. 1 - 18, Sept. 2021.2076-0817https://www.arca.fiocruz.br/handle/icict/5091910.3390/pathogens10101250engMDPIFebre QImunoinformáticaEpítopo de célula BSorodiagnósticoPeptídeos sintéticosQ feverImmunoinformaticsB-cell epitopeSerodiagnosisSynthetic peptidesIdentification of Immunogenic Linear B-Cell Epitopes in C. burnetii Outer Membrane Proteins Using Immunoinformatics Approaches Reveals Potential Targets of Persistent Infectionsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos. Laboratório de Tecnologia de Anticorpos Monoclonais. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos. Laboratório de Tecnologia de Anticorpos Monoclonais. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos. Laboratório de Tecnologia de Anticorpos Monoclonais. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos. Projeto de Implantação de Planta Piloto. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunoparasitologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hantaviroses e Rickettsioses. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hantaviroses e Rickettsioses. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hantaviroses e Rickettsioses. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hantaviroses e Rickettsioses. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hantaviroses e Rickettsioses. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos. Laboratório de Tecnologia de Anticorpos Monoclonais. Rio de Janeiro, RJ, Brasil.Coxiella burnetii is a global, highly infectious intracellular bacterium, able to infect a wide range of hosts and to persist for months in the environment. It is the etiological agent of Q fever— a zoonosis of global priority. Currently, there are no national surveillance data on C. burnetii’s seroprevalence for any South American country, reinforcing the necessity of developing novel and inexpensive serological tools to monitor the prevalence of infections among humans and animals— especially cattle, goats, and sheep. In this study, we used immunoinformatics and computational biology tools to predict specific linear B-cell epitopes in three C. burnetii outer membrane proteins: OMP-H (CBU_0612), Com-1 (CBU_1910), and OMP-P1 (CBU_0311). Furthermore, predicted epitopes were tested by ELISA, as synthetic peptides, against samples of patients reactive to C. burnetii in indirect immunofluorescence assay, in order to evaluate their natural immunogenicity. In this way, two linear B-cell epitopes were identified in each studied protein (OMP-H(51–59), OMP-H(91–106), Com-1(57–76), Com-1(191–206), OMP-P1(197–209), and OMP-P1(215–227)); all of them were confirmed as naturally immunogenic by the presence of specific antibodies in 77% of studied patients against at least one of the identified epitopes. Remarkably, a higher frequency of endocarditis cases was observed among patients who presented an intense humoral response to OMP-H and Com-1 epitopes. These data confirm that immunoinformatics applied to the identification of specific B-cell epitopes can be an effective strategy to improve and accelerate the development of surveillance tools against neglected diseases.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/50919/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALJosueCostaLimaJr_TatianaRozental_etal_IOC_2021.pdfJosueCostaLimaJr_TatianaRozental_etal_IOC_2021.pdfapplication/pdf1304886https://www.arca.fiocruz.br/bitstream/icict/50919/2/JosueCostaLimaJr_TatianaRozental_etal_IOC_2021.pdf1308a487de5a80152125a30f86e1465dMD52icict/509192022-06-24 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dc.title.pt_BR.fl_str_mv Identification of Immunogenic Linear B-Cell Epitopes in C. burnetii Outer Membrane Proteins Using Immunoinformatics Approaches Reveals Potential Targets of Persistent Infections
title Identification of Immunogenic Linear B-Cell Epitopes in C. burnetii Outer Membrane Proteins Using Immunoinformatics Approaches Reveals Potential Targets of Persistent Infections
spellingShingle Identification of Immunogenic Linear B-Cell Epitopes in C. burnetii Outer Membrane Proteins Using Immunoinformatics Approaches Reveals Potential Targets of Persistent Infections
Fontes, Sílvia da Silva
Febre Q
Imunoinformática
Epítopo de célula B
Sorodiagnóstico
Peptídeos sintéticos
Q fever
Immunoinformatics
B-cell epitope
Serodiagnosis
Synthetic peptides
title_short Identification of Immunogenic Linear B-Cell Epitopes in C. burnetii Outer Membrane Proteins Using Immunoinformatics Approaches Reveals Potential Targets of Persistent Infections
title_full Identification of Immunogenic Linear B-Cell Epitopes in C. burnetii Outer Membrane Proteins Using Immunoinformatics Approaches Reveals Potential Targets of Persistent Infections
title_fullStr Identification of Immunogenic Linear B-Cell Epitopes in C. burnetii Outer Membrane Proteins Using Immunoinformatics Approaches Reveals Potential Targets of Persistent Infections
title_full_unstemmed Identification of Immunogenic Linear B-Cell Epitopes in C. burnetii Outer Membrane Proteins Using Immunoinformatics Approaches Reveals Potential Targets of Persistent Infections
title_sort Identification of Immunogenic Linear B-Cell Epitopes in C. burnetii Outer Membrane Proteins Using Immunoinformatics Approaches Reveals Potential Targets of Persistent Infections
author Fontes, Sílvia da Silva
author_facet Fontes, Sílvia da Silva
Maia, Fernanda de Moraes
Ataides, Laura Santa’Anna
Conte, Fernando Paiva
Lima Junior, Josué da Costa
Rozental, Tatiana
Assis, Matheus Ribeiro da Silva
Pessoa Júnior, Adonai Alvino
Fernandes, Jorlan
Lemos, Elba Regina Sampaio de
Silva, Rodrigo Nunes Rodrigues da
author_role author
author2 Maia, Fernanda de Moraes
Ataides, Laura Santa’Anna
Conte, Fernando Paiva
Lima Junior, Josué da Costa
Rozental, Tatiana
Assis, Matheus Ribeiro da Silva
Pessoa Júnior, Adonai Alvino
Fernandes, Jorlan
Lemos, Elba Regina Sampaio de
Silva, Rodrigo Nunes Rodrigues da
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Fontes, Sílvia da Silva
Maia, Fernanda de Moraes
Ataides, Laura Santa’Anna
Conte, Fernando Paiva
Lima Junior, Josué da Costa
Rozental, Tatiana
Assis, Matheus Ribeiro da Silva
Pessoa Júnior, Adonai Alvino
Fernandes, Jorlan
Lemos, Elba Regina Sampaio de
Silva, Rodrigo Nunes Rodrigues da
dc.subject.other.pt_BR.fl_str_mv Febre Q
Imunoinformática
Epítopo de célula B
Sorodiagnóstico
Peptídeos sintéticos
topic Febre Q
Imunoinformática
Epítopo de célula B
Sorodiagnóstico
Peptídeos sintéticos
Q fever
Immunoinformatics
B-cell epitope
Serodiagnosis
Synthetic peptides
dc.subject.en.pt_BR.fl_str_mv Q fever
Immunoinformatics
B-cell epitope
Serodiagnosis
Synthetic peptides
description Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos. Laboratório de Tecnologia de Anticorpos Monoclonais. Rio de Janeiro, RJ, Brasil.
publishDate 2021
dc.date.issued.fl_str_mv 2021
dc.date.accessioned.fl_str_mv 2022-01-27T17:54:48Z
dc.date.available.fl_str_mv 2022-01-27T17:54:48Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv FONTES, Sílvia da Silva et al. Identification of Immunogenic Linear B-Cell Epitopes in C. burnetii Outer Membrane Proteins Using Immunoinformatics Approaches Reveals Potential Targets of Persistent Infections. Pathogens, v. 10, 1250, p. 1 - 18, Sept. 2021.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/50919
dc.identifier.issn.pt_BR.fl_str_mv 2076-0817
dc.identifier.doi.none.fl_str_mv 10.3390/pathogens10101250
identifier_str_mv FONTES, Sílvia da Silva et al. Identification of Immunogenic Linear B-Cell Epitopes in C. burnetii Outer Membrane Proteins Using Immunoinformatics Approaches Reveals Potential Targets of Persistent Infections. Pathogens, v. 10, 1250, p. 1 - 18, Sept. 2021.
2076-0817
10.3390/pathogens10101250
url https://www.arca.fiocruz.br/handle/icict/50919
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/50919/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/50919/2/JosueCostaLimaJr_TatianaRozental_etal_IOC_2021.pdf
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repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
repository.mail.fl_str_mv repositorio.arca@fiocruz.br
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