Biological characterization and chemokine receptor usage of HIV type 1 isolates prevalent in Brazil.

Detalhes bibliográficos
Autor(a) principal: Ferraro, Geraldo Argolo
Data de Publicação: 2001
Outros Autores: Mello, Marco Antônio Gomes, Sutmöller, Frits, Van Weyenbergh, Johan Jozef Rosa Maria, Brazilian Network For HIV isolation and characterizationI, Shindo, Nice, Castro Filho, Bernardo Galvão, Bou-Habib, Dumith Chequer
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/8146
Resumo: Escola Bahiana de Medicina e Saúde Pública. Fundação para o Desenvolvimento das Ciências. Salvador, BA, Brasil / UNESCO. Ministério da Saude. CN/DST/AIDS. Salvador, BA, Brasil
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spelling Ferraro, Geraldo ArgoloMello, Marco Antônio GomesSutmöller, FritsVan Weyenbergh, Johan Jozef Rosa MariaBrazilian Network For HIV isolation and characterizationIShindo, NiceCastro Filho, Bernardo GalvãoBou-Habib, Dumith Chequer2014-08-06T19:04:13Z2014-08-06T19:04:13Z2001FERRARO, G. A. et al. Biological characterization and chemokine receptor usage of HIV type 1 isolates prevalent in Brazil. AIDS Research and Human Retroviruses, v. 17, n. 13, p. 1241-1247, 2001.0889-2229https://www.arca.fiocruz.br/handle/icict/8146engMary Ann Liebert, IncBiological characterization and chemokine receptor usage of HIV type 1 isolates prevalent in Brazil.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleEscola Bahiana de Medicina e Saúde Pública. Fundação para o Desenvolvimento das Ciências. Salvador, BA, Brasil / UNESCO. Ministério da Saude. CN/DST/AIDS. Salvador, BA, BrasilEscola Bahiana de Medicina e Saúde Pública. Fundação para o Desenvolvimento das Ciências. Salvador, BA, Brasil / UNESCO. Ministério da Saude. CN/DST/AIDS. Salvador, BA, BrasilFundação Oswaldo Cruz. Escola Nacional de Saúde Pública. Rio de Janeiro, RJ, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório Integrado de Microbiologia e Imunorregulação. Salvador, BA, BrasilFundação Oswaldo Cruz. Salvador, BA, BrasilEscola Bahiana de Medicina e Saúde Pública. Fundação para o Desenvolvimento das Ciências. Salvador, BA, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório Avançado de Saúde Pública. Salvador, BA, BrasilEscola Bahiana de Medicina e Saúde Pública. Fundação para o Desenvolvimento das Ciências. Salvador, BA, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório Avançado de Saúde Pública. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório Avançado de Saúde Pública. Salvador, BA, BrasilThe human immunodeficiency virus type 1 (HIV-1), the etiological agent of the acquired immunodeficiency syndrome (AIDS), shows a variety of biological properties, which may constitute an obstacle to development of effective vaccines or antiretroviral therapy. To characterize Brazilian strains of HIV-1, we studied 24 viruses isolated from blood samples of HIV-1-positive patients from different regions of the country. To examine the cell tropism and the virus ability to form syncytia, primary macrophages and the CD41 T cell line MT-2 were infected with these viruses. We found that 22 isolates replicated well in macrophages (macrophage-tropic isolates), 2 infected only MT-2 cells (T cell line tropic variants), while 6 of them grew in both cells. We found 8 syncytium-inducing (SI) and 16 non-SI (NSI) isolates. Continuous cultures of 18 isolates were established in the CCR51 /CXCR41 cell line PM-1, and SI/NSI features of these viruses were confirmed by cell fusion assay with uninfected CD41 T cell lines (PM-1, MT-2, H9, and SUP-T1). The coreceptor usage of 18 isolates was investigated by infecting U87 cells transfected with CD4 and chemokine receptors, and we found that 11 isolates infected only CCR51 cells, 3 only CXCR41 cells, whereas 4 used both coreceptors. We also observed that X4 isolates were more sensitive to neutralization by dextran sulfate than R5 or R5X4 viruses. Our findings show that the Brazilian isolates are phenotypically similar to those prevalent in other regions, which could mean that therapeutic strategies based on HIV-1 phenotypic properties would be efficient in Brazil, as in other countries.Infecções por HIV/virologiaHIV-1/isolamento & purificaçãoHIV-1/metabolismoReceptores CCR5/metabolismoBrasil/epidemiologiaLinhagem CelularSulfato de Dextrana/farmacologiaCélulas Gigantes/efeitos de drogasInfecções por HIV/epidemiologiaHIV-1/efeitos de drogasHIV-1/genéticaHumanosMacrófagos/efeitos de drogasMacrófagos/virologiaEspecificidade por Substratoinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALFerraro GA Biological characterization....pdfFerraro GA Biological characterization....pdfapplication/pdf165242https://www.arca.fiocruz.br/bitstream/icict/8146/1/Ferraro%20GA%20Biological%20characterization....pdfbb755db72ba2ea81386e1608dfefe870MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://www.arca.fiocruz.br/bitstream/icict/8146/2/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD52TEXTFerraro GA Biological characterization....pdf.txtFerraro GA Biological characterization....pdf.txtExtracted texttext/plain37525https://www.arca.fiocruz.br/bitstream/icict/8146/3/Ferraro%20GA%20Biological%20characterization....pdf.txt736bcfedc5fe288d017c40aff5a218acMD53icict/81462023-03-15 14:32:59.417oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-15T17:32:59Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Biological characterization and chemokine receptor usage of HIV type 1 isolates prevalent in Brazil.
title Biological characterization and chemokine receptor usage of HIV type 1 isolates prevalent in Brazil.
spellingShingle Biological characterization and chemokine receptor usage of HIV type 1 isolates prevalent in Brazil.
Ferraro, Geraldo Argolo
Infecções por HIV/virologia
HIV-1/isolamento & purificação
HIV-1/metabolismo
Receptores CCR5/metabolismo
Brasil/epidemiologia
Linhagem Celular
Sulfato de Dextrana/farmacologia
Células Gigantes/efeitos de drogas
Infecções por HIV/epidemiologia
HIV-1/efeitos de drogas
HIV-1/genética
Humanos
Macrófagos/efeitos de drogas
Macrófagos/virologia
Especificidade por Substrato
title_short Biological characterization and chemokine receptor usage of HIV type 1 isolates prevalent in Brazil.
title_full Biological characterization and chemokine receptor usage of HIV type 1 isolates prevalent in Brazil.
title_fullStr Biological characterization and chemokine receptor usage of HIV type 1 isolates prevalent in Brazil.
title_full_unstemmed Biological characterization and chemokine receptor usage of HIV type 1 isolates prevalent in Brazil.
title_sort Biological characterization and chemokine receptor usage of HIV type 1 isolates prevalent in Brazil.
author Ferraro, Geraldo Argolo
author_facet Ferraro, Geraldo Argolo
Mello, Marco Antônio Gomes
Sutmöller, Frits
Van Weyenbergh, Johan Jozef Rosa Maria
Brazilian Network For HIV isolation and characterizationI
Shindo, Nice
Castro Filho, Bernardo Galvão
Bou-Habib, Dumith Chequer
author_role author
author2 Mello, Marco Antônio Gomes
Sutmöller, Frits
Van Weyenbergh, Johan Jozef Rosa Maria
Brazilian Network For HIV isolation and characterizationI
Shindo, Nice
Castro Filho, Bernardo Galvão
Bou-Habib, Dumith Chequer
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Ferraro, Geraldo Argolo
Mello, Marco Antônio Gomes
Sutmöller, Frits
Van Weyenbergh, Johan Jozef Rosa Maria
Brazilian Network For HIV isolation and characterizationI
Shindo, Nice
Castro Filho, Bernardo Galvão
Bou-Habib, Dumith Chequer
dc.subject.decs.pt_BR.fl_str_mv Infecções por HIV/virologia
HIV-1/isolamento & purificação
HIV-1/metabolismo
Receptores CCR5/metabolismo
Brasil/epidemiologia
Linhagem Celular
Sulfato de Dextrana/farmacologia
Células Gigantes/efeitos de drogas
Infecções por HIV/epidemiologia
HIV-1/efeitos de drogas
HIV-1/genética
Humanos
Macrófagos/efeitos de drogas
Macrófagos/virologia
Especificidade por Substrato
topic Infecções por HIV/virologia
HIV-1/isolamento & purificação
HIV-1/metabolismo
Receptores CCR5/metabolismo
Brasil/epidemiologia
Linhagem Celular
Sulfato de Dextrana/farmacologia
Células Gigantes/efeitos de drogas
Infecções por HIV/epidemiologia
HIV-1/efeitos de drogas
HIV-1/genética
Humanos
Macrófagos/efeitos de drogas
Macrófagos/virologia
Especificidade por Substrato
description Escola Bahiana de Medicina e Saúde Pública. Fundação para o Desenvolvimento das Ciências. Salvador, BA, Brasil / UNESCO. Ministério da Saude. CN/DST/AIDS. Salvador, BA, Brasil
publishDate 2001
dc.date.issued.fl_str_mv 2001
dc.date.accessioned.fl_str_mv 2014-08-06T19:04:13Z
dc.date.available.fl_str_mv 2014-08-06T19:04:13Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv FERRARO, G. A. et al. Biological characterization and chemokine receptor usage of HIV type 1 isolates prevalent in Brazil. AIDS Research and Human Retroviruses, v. 17, n. 13, p. 1241-1247, 2001.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/8146
dc.identifier.issn.none.fl_str_mv 0889-2229
identifier_str_mv FERRARO, G. A. et al. Biological characterization and chemokine receptor usage of HIV type 1 isolates prevalent in Brazil. AIDS Research and Human Retroviruses, v. 17, n. 13, p. 1241-1247, 2001.
0889-2229
url https://www.arca.fiocruz.br/handle/icict/8146
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Mary Ann Liebert, Inc
publisher.none.fl_str_mv Mary Ann Liebert, Inc
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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