Molecular Karyotype and Chromosomal Localization of Genes Encoding ß-tubulin, Cysteine Proteinase, hsp 70 and Actin in Trypanosoma rangeli
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2001 |
| Outros Autores: | , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/44111 |
Resumo: | Universidade do Estado do Rio de Janeiro. Departamento de Biologia Celular e Genética. Rio de Janeiro, RJ, Brasil. |
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Toaldo, C. B.Steindel, M.Spusa, M. A.Tavares, C. C.2020-10-22T13:46:22Z2020-10-22T13:46:22Z2001TOALDO, C. B. et al. Molecular Karyotype and Chromosomal Localization of Genes Encoding β-tubulin, Cysteine Proteinase, hsp 70 and Actin in Trypanosoma rangeli. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 96, n. 1, p. 113-121, Jan. 2001.0074-0276https://www.arca.fiocruz.br/handle/icict/4411110.1590/S0074-027620010001000131678-8060engFundação Oswaldo Cruz. Instituto Oswaldo Cruz.Trypanosoma rangeliCariótipoEletroforese em gel de campo pulsadoGenes domésticosVariabilidade genéticaFenogramaTrypanosoma rangeliKaryotypePulsed field gel electrophoresisHousekeeping genesGenetic variabilityPhenogramMolecular Karyotype and Chromosomal Localization of Genes Encoding ß-tubulin, Cysteine Proteinase, hsp 70 and Actin in Trypanosoma rangeliinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade do Estado do Rio de Janeiro. Departamento de Biologia Celular e Genética. Rio de Janeiro, RJ, Brasil.Universidade Federal de Santa Catarina. Departamento de Microbiologia e Parasitologia., Florianópolis, SC, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Entomologia. Coleção de Tripanosomatídeos. Rio de Janeiro, RJ, Brasil.Universidade do Estado do Rio de Janeiro. Departamento de Biologia Celular e Genética. Rio de Janeiro, RJ, Brasil.The molecular karyotype of nine Trypanosoma rangeli strains was analyzed by contour-clamped homogeneous electric field electrophoresis, followed by the chromosomal localization of β-tubulin, cysteine proteinase, 70 kDa heat shock protein (hsp 70) and actin genes. The T. rangeli strains were isolated from either insects or mammals from El Salvador, Honduras, Venezuela, Colombia, Panama and southern Brazil. Also, T. cruzi CL-Brener clone was included for comparison. Despite the great similarity observed among strains from Brazil, the molecular karyotype of all T. rangeli strains analyzed revealed extensive chromosome polymorphism. In addition, it was possible to distinguish T. rangeli from T. cruzi by the chromosomal DNA electrophoresis pattern. The localization of β-tubulin genes revealed differences among T. rangeli strains and confirmed the similarity between the isolates from Brazil. Hybridization assays using probes directed to the cysteine proteinase, hsp 70 and actin genes discriminated T. rangeli from T. cruzi, proving that these genes are useful molecular markers for the differential diagnosis between these two species. Numerical analysis based on the molecular karyotype data revealed a high degree of polymorphism among T. rangeli strains isolated from southern Brazil and strains isolated from Central and the northern South America. The T. cruzi reference strain was not clustered with any T. rangeli strain.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/44111/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALMASouza_etal_IOC_2001.pdfMASouza_etal_IOC_2001.pdfapplication/pdf400257https://www.arca.fiocruz.br/bitstream/icict/44111/2/MASouza_etal_IOC_2001.pdf51179c6fcdcf7759dca27b2dce902cbdMD52TEXTMASouza_etal_IOC_2001.pdf.txtMASouza_etal_IOC_2001.pdf.txtExtracted texttext/plain31074https://www.arca.fiocruz.br/bitstream/icict/44111/3/MASouza_etal_IOC_2001.pdf.txtd7049060c2b18ea807dd4c6443edbf1bMD53icict/441112020-10-23 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| dc.title.pt_BR.fl_str_mv |
Molecular Karyotype and Chromosomal Localization of Genes Encoding ß-tubulin, Cysteine Proteinase, hsp 70 and Actin in Trypanosoma rangeli |
| title |
Molecular Karyotype and Chromosomal Localization of Genes Encoding ß-tubulin, Cysteine Proteinase, hsp 70 and Actin in Trypanosoma rangeli |
| spellingShingle |
Molecular Karyotype and Chromosomal Localization of Genes Encoding ß-tubulin, Cysteine Proteinase, hsp 70 and Actin in Trypanosoma rangeli Toaldo, C. B. Trypanosoma rangeli Cariótipo Eletroforese em gel de campo pulsado Genes domésticos Variabilidade genética Fenograma Trypanosoma rangeli Karyotype Pulsed field gel electrophoresis Housekeeping genes Genetic variability Phenogram |
| title_short |
Molecular Karyotype and Chromosomal Localization of Genes Encoding ß-tubulin, Cysteine Proteinase, hsp 70 and Actin in Trypanosoma rangeli |
| title_full |
Molecular Karyotype and Chromosomal Localization of Genes Encoding ß-tubulin, Cysteine Proteinase, hsp 70 and Actin in Trypanosoma rangeli |
| title_fullStr |
Molecular Karyotype and Chromosomal Localization of Genes Encoding ß-tubulin, Cysteine Proteinase, hsp 70 and Actin in Trypanosoma rangeli |
| title_full_unstemmed |
Molecular Karyotype and Chromosomal Localization of Genes Encoding ß-tubulin, Cysteine Proteinase, hsp 70 and Actin in Trypanosoma rangeli |
| title_sort |
Molecular Karyotype and Chromosomal Localization of Genes Encoding ß-tubulin, Cysteine Proteinase, hsp 70 and Actin in Trypanosoma rangeli |
| author |
Toaldo, C. B. |
| author_facet |
Toaldo, C. B. Steindel, M. Spusa, M. A. Tavares, C. C. |
| author_role |
author |
| author2 |
Steindel, M. Spusa, M. A. Tavares, C. C. |
| author2_role |
author author author |
| dc.contributor.author.fl_str_mv |
Toaldo, C. B. Steindel, M. Spusa, M. A. Tavares, C. C. |
| dc.subject.other.pt_BR.fl_str_mv |
Trypanosoma rangeli Cariótipo Eletroforese em gel de campo pulsado Genes domésticos Variabilidade genética Fenograma |
| topic |
Trypanosoma rangeli Cariótipo Eletroforese em gel de campo pulsado Genes domésticos Variabilidade genética Fenograma Trypanosoma rangeli Karyotype Pulsed field gel electrophoresis Housekeeping genes Genetic variability Phenogram |
| dc.subject.en.pt_BR.fl_str_mv |
Trypanosoma rangeli Karyotype Pulsed field gel electrophoresis Housekeeping genes Genetic variability Phenogram |
| description |
Universidade do Estado do Rio de Janeiro. Departamento de Biologia Celular e Genética. Rio de Janeiro, RJ, Brasil. |
| publishDate |
2001 |
| dc.date.issued.fl_str_mv |
2001 |
| dc.date.accessioned.fl_str_mv |
2020-10-22T13:46:22Z |
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2020-10-22T13:46:22Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
| dc.identifier.citation.fl_str_mv |
TOALDO, C. B. et al. Molecular Karyotype and Chromosomal Localization of Genes Encoding β-tubulin, Cysteine Proteinase, hsp 70 and Actin in Trypanosoma rangeli. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 96, n. 1, p. 113-121, Jan. 2001. |
| dc.identifier.uri.fl_str_mv |
https://www.arca.fiocruz.br/handle/icict/44111 |
| dc.identifier.issn.pt_BR.fl_str_mv |
0074-0276 |
| dc.identifier.doi.none.fl_str_mv |
10.1590/S0074-02762001000100013 |
| dc.identifier.eissn.none.fl_str_mv |
1678-8060 |
| identifier_str_mv |
TOALDO, C. B. et al. Molecular Karyotype and Chromosomal Localization of Genes Encoding β-tubulin, Cysteine Proteinase, hsp 70 and Actin in Trypanosoma rangeli. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 96, n. 1, p. 113-121, Jan. 2001. 0074-0276 10.1590/S0074-02762001000100013 1678-8060 |
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eng |
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eng |
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info:eu-repo/semantics/openAccess |
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openAccess |
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Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. |
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Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. |
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