Selecting the right gate to identify relevant cells for your assay: a study of thioglycollate-elicited peritoneal exudate cells in mice
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2017 |
| Outros Autores: | , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/24925 |
Resumo: | Brazilian Ministry of Science and Technology, the Brazilian National Research Council (CNPq, Grant No. 474548/2009-0). Committee of Ethics in the Use of Animals of the CPqGMFIOCRUZ (CEUA, License N. 018/2009). |
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Hermida, Micely D El ReyMalta, RafaelaSantos, Marcos D. P. C. de SdosSantos, Washington Luis Conrado2018-02-23T14:11:57Z2018-02-23T14:11:57Z2017HERMIDA, M. D El R. et al. Selecting the right gate to identify relevant cells for your assay: a study of thioglycollate-elicited peritoneal exudate cells in mice. BMC Research Notes, v. 10, p. 695, 2017.1756-0500https://www.arca.fiocruz.br/handle/icict/2492510.1186/s13104-017-3019-5Brazilian Ministry of Science and Technology, the Brazilian National Research Council (CNPq, Grant No. 474548/2009-0). Committee of Ethics in the Use of Animals of the CPqGMFIOCRUZ (CEUA, License N. 018/2009).Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Patologia e Biointervenção. Salvador, BA, BrasilFundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Patologia e Biointervenção. Salvador, BA, BrasilFundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Patologia e Biointervenção. Salvador, BA, BrasilFundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Patologia e Biointervenção. Salvador, BA, BrasilIn this study, we investigate the diversity and modulation of leukocyte populations represented in the gates defined by size and granularity at different time points of thioglycollate-induced peritonitis in mouse. Results: The inflammatory cells were distributed into four regions (R1–R4) of a data plot graph defined by cell size and granularity. R1 and R2 contained agranular cells that were small in size and predominately included T ( CD3+) lymphocytes along with B ( B220+) lymphocytes. Macrophages (F4/80+) were the predominant cells found in the R3 region. However, these cells were present in all regions, albeit at a lower frequency in R1 and R2. Granulocytes ( Gr1+) were mainly distributed in R3 and R4. The wide distribution of F4/80+ and Gr1+ cells may reflect the recruitment and activation state of the different macrophage and granulocyte populations. Based on these observations, size and granularity may contribute to an initial step in the analysis and sorting of thioglycollate-elicited peritoneal exudate cells. However, the developmental stage and cell activation state may interfere with cell segregation using size and granularity as parameters.engBioMed CentralCitometria de fluxoPeritoniteTioglicolatosCélulasMacrófagosFlow cytometryPeritoneal exudate cells,Thioglycollate stimuliCell sizeGranularitySelecting the right gate to identify relevant cells for your assay: a study of thioglycollate-elicited peritoneal exudate cells in miceinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/24925/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALHermida MD Selecting the right gate to identify .....pdfHermida MD Selecting the right gate to identify 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| dc.title.pt_BR.fl_str_mv |
Selecting the right gate to identify relevant cells for your assay: a study of thioglycollate-elicited peritoneal exudate cells in mice |
| title |
Selecting the right gate to identify relevant cells for your assay: a study of thioglycollate-elicited peritoneal exudate cells in mice |
| spellingShingle |
Selecting the right gate to identify relevant cells for your assay: a study of thioglycollate-elicited peritoneal exudate cells in mice Hermida, Micely D El Rey Citometria de fluxo Peritonite Tioglicolatos Células Macrófagos Flow cytometry Peritoneal exudate cells, Thioglycollate stimuli Cell size Granularity |
| title_short |
Selecting the right gate to identify relevant cells for your assay: a study of thioglycollate-elicited peritoneal exudate cells in mice |
| title_full |
Selecting the right gate to identify relevant cells for your assay: a study of thioglycollate-elicited peritoneal exudate cells in mice |
| title_fullStr |
Selecting the right gate to identify relevant cells for your assay: a study of thioglycollate-elicited peritoneal exudate cells in mice |
| title_full_unstemmed |
Selecting the right gate to identify relevant cells for your assay: a study of thioglycollate-elicited peritoneal exudate cells in mice |
| title_sort |
Selecting the right gate to identify relevant cells for your assay: a study of thioglycollate-elicited peritoneal exudate cells in mice |
| author |
Hermida, Micely D El Rey |
| author_facet |
Hermida, Micely D El Rey Malta, Rafaela Santos, Marcos D. P. C. de S dosSantos, Washington Luis Conrado |
| author_role |
author |
| author2 |
Malta, Rafaela Santos, Marcos D. P. C. de S dosSantos, Washington Luis Conrado |
| author2_role |
author author author |
| dc.contributor.author.fl_str_mv |
Hermida, Micely D El Rey Malta, Rafaela Santos, Marcos D. P. C. de S dosSantos, Washington Luis Conrado |
| dc.subject.other.pt_BR.fl_str_mv |
Citometria de fluxo Peritonite Tioglicolatos Células Macrófagos |
| topic |
Citometria de fluxo Peritonite Tioglicolatos Células Macrófagos Flow cytometry Peritoneal exudate cells, Thioglycollate stimuli Cell size Granularity |
| dc.subject.en.pt_BR.fl_str_mv |
Flow cytometry Peritoneal exudate cells, Thioglycollate stimuli Cell size Granularity |
| description |
Brazilian Ministry of Science and Technology, the Brazilian National Research Council (CNPq, Grant No. 474548/2009-0). Committee of Ethics in the Use of Animals of the CPqGMFIOCRUZ (CEUA, License N. 018/2009). |
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2017 |
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2017 |
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2018-02-23T14:11:57Z |
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2018-02-23T14:11:57Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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HERMIDA, M. D El R. et al. Selecting the right gate to identify relevant cells for your assay: a study of thioglycollate-elicited peritoneal exudate cells in mice. BMC Research Notes, v. 10, p. 695, 2017. |
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https://www.arca.fiocruz.br/handle/icict/24925 |
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1756-0500 |
| dc.identifier.doi.none.fl_str_mv |
10.1186/s13104-017-3019-5 |
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HERMIDA, M. D El R. et al. Selecting the right gate to identify relevant cells for your assay: a study of thioglycollate-elicited peritoneal exudate cells in mice. BMC Research Notes, v. 10, p. 695, 2017. 1756-0500 10.1186/s13104-017-3019-5 |
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