Expression of active trypsin-like serine peptidases in the midgut of sugar-feeding female Anopheles aquasalis

Detalhes bibliográficos
Autor(a) principal: Lopes, Geovane Dias
Data de Publicação: 2015
Outros Autores: Veloso, Andre Borges, Vahia, Leonardo Saboia, Domont, Gilberto B., Britto, Constança, Cuervo, Patricia, Jesus, José Batista de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/13372
Resumo: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular e Doenças Endêmicas. Rio de Janeiro, RJ, Brasil.
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spelling Lopes, Geovane DiasVeloso, Andre BorgesVahia, Leonardo SaboiaDomont, Gilberto B.Britto, ConstançaCuervo, PatriciaJesus, José Batista de2016-03-29T11:04:46Z2016-03-29T11:04:46Z2015LOPES, Geovane Dias; et al. Expression of active trypsin-like serine peptidases in the midgut of sugar-feeding female Anopheles aquasalis. Parasites & Vectors, v.8:296, 10p, 2015.1756-3305https://www.arca.fiocruz.br/handle/icict/1337210.1186/s13071-015-0908-0engBioMed CentralExpression of active trypsin-like serine peptidases in the midgut of sugar-feeding female Anopheles aquasalisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular e Doenças Endêmicas. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular e Doenças Endêmicas. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Leishmaniose. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Departamento de Química. Laboratório de Química de Proteínas. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular e Doenças Endêmicas. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Leishmaniose. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular e Doenças Endêmicas. Rio de Janeiro, RJ, Brasil / Universidade Federal de São João Del Rey. Faculdade de Medicina. Departamento de Medicina. São João del Rey, MG, Brasil.Background: Anopheles aquasalis is a dipteran of the family Culicidae that is widely distributed in the coastal regions of South and Central America. This species acts as a vector of Plasmodium vivax, an important etiological agent of malaria, which represents a serious public health problem. In mosquitoes, trypsin-like serine proteases are important in blood meal digestion, immune responses and reproductive functions. The study of peptidases expressed in the mosquito midgut is essential to understanding the mechanisms of parasite-host interaction and the physiological process of nutrient digestion. Methods: Our study aimed to identify and characterize the proteolytic activities in the midgut of sugar-fed An. aquasalis females using zymographic analyses (substrate-SDS-PAGE), in-solution assays and mass spectrometry. Results: Here, we used a zymographic analysis to further biochemically characterize the proteolytic profile of the midgut of sugar-feeding An. aquasalis females. The trypsin peptidases migrated between ~17 and ~76 kDa and displayed higher proteolytic activities between pH 7.5 and 10 and at temperatures between 37 °C and 50 °C. Four putative trypsin-like serine peptidases were identified using mass spectrometry and data mining. The molecular masses of these peptidases were similar to those observed using zymography, which suggested that these peptidases could be responsible for some of the observed proteolytic bands. Conclusions: Taken together, our results contribute to the gene annotation of the unknown genome of this species, to the tissue location of these peptidases, and to the functional prediction of these crucial enzymes, which all impact further studies of this species.Anopheles aquasalisMidgutZymographyTrypsin-like serine peptidasesProteomicsMass spectrometryEspectrometria de MassasProteômicaDípterosSerine proteasesPeptídeo Hidrolasesinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZTHUMBNAILgeovane_lopes_etal_IOC_2015.pdf.jpggeovane_lopes_etal_IOC_2015.pdf.jpgGenerated Thumbnailimage/jpeg1701https://www.arca.fiocruz.br/bitstream/icict/13372/4/geovane_lopes_etal_IOC_2015.pdf.jpg91194ea08a5b66e1a488b5b3f9a7763aMD54LICENSElicense.txtlicense.txttext/plain; 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dc.title.pt_BR.fl_str_mv Expression of active trypsin-like serine peptidases in the midgut of sugar-feeding female Anopheles aquasalis
title Expression of active trypsin-like serine peptidases in the midgut of sugar-feeding female Anopheles aquasalis
spellingShingle Expression of active trypsin-like serine peptidases in the midgut of sugar-feeding female Anopheles aquasalis
Lopes, Geovane Dias
Anopheles aquasalis
Midgut
Zymography
Trypsin-like serine peptidases
Proteomics
Mass spectrometry
Espectrometria de Massas
Proteômica
Dípteros
Serine proteases
Peptídeo Hidrolases
title_short Expression of active trypsin-like serine peptidases in the midgut of sugar-feeding female Anopheles aquasalis
title_full Expression of active trypsin-like serine peptidases in the midgut of sugar-feeding female Anopheles aquasalis
title_fullStr Expression of active trypsin-like serine peptidases in the midgut of sugar-feeding female Anopheles aquasalis
title_full_unstemmed Expression of active trypsin-like serine peptidases in the midgut of sugar-feeding female Anopheles aquasalis
title_sort Expression of active trypsin-like serine peptidases in the midgut of sugar-feeding female Anopheles aquasalis
author Lopes, Geovane Dias
author_facet Lopes, Geovane Dias
Veloso, Andre Borges
Vahia, Leonardo Saboia
Domont, Gilberto B.
Britto, Constança
Cuervo, Patricia
Jesus, José Batista de
author_role author
author2 Veloso, Andre Borges
Vahia, Leonardo Saboia
Domont, Gilberto B.
Britto, Constança
Cuervo, Patricia
Jesus, José Batista de
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Lopes, Geovane Dias
Veloso, Andre Borges
Vahia, Leonardo Saboia
Domont, Gilberto B.
Britto, Constança
Cuervo, Patricia
Jesus, José Batista de
dc.subject.en.pt_BR.fl_str_mv Anopheles aquasalis
Midgut
Zymography
Trypsin-like serine peptidases
Proteomics
Mass spectrometry
topic Anopheles aquasalis
Midgut
Zymography
Trypsin-like serine peptidases
Proteomics
Mass spectrometry
Espectrometria de Massas
Proteômica
Dípteros
Serine proteases
Peptídeo Hidrolases
dc.subject.decs.pt_BR.fl_str_mv Espectrometria de Massas
Proteômica
Dípteros
Serine proteases
Peptídeo Hidrolases
description Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular e Doenças Endêmicas. Rio de Janeiro, RJ, Brasil.
publishDate 2015
dc.date.issued.fl_str_mv 2015
dc.date.accessioned.fl_str_mv 2016-03-29T11:04:46Z
dc.date.available.fl_str_mv 2016-03-29T11:04:46Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv LOPES, Geovane Dias; et al. Expression of active trypsin-like serine peptidases in the midgut of sugar-feeding female Anopheles aquasalis. Parasites & Vectors, v.8:296, 10p, 2015.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/13372
dc.identifier.issn.none.fl_str_mv 1756-3305
dc.identifier.doi.none.fl_str_mv 10.1186/s13071-015-0908-0
identifier_str_mv LOPES, Geovane Dias; et al. Expression of active trypsin-like serine peptidases in the midgut of sugar-feeding female Anopheles aquasalis. Parasites & Vectors, v.8:296, 10p, 2015.
1756-3305
10.1186/s13071-015-0908-0
url https://www.arca.fiocruz.br/handle/icict/13372
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv BioMed Central
publisher.none.fl_str_mv BioMed Central
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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