Autophagy-associated IL-15 production is involved in the pathogenesis of leprosy type 1 reaction
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2021 |
| Outros Autores: | , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/50126 |
Resumo: | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil. |
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Silva, Bruno Jorge de AndradeBittencourt, Tamiris LameiraLeal-Calvo, ThyagoMendes, Mayara AbudPrata, Rhana Berto da SilvaBarbosa, Mayara Garcia de MattosAndrade, Priscila RibeiroCôrte-Real, SuzanaSilva, Gilberto Marcelo Sperandio daMoraes, Milton OsórioSarno, Euzenir NunesPinheiro, Roberta Olmo2021-12-01T13:54:35Z2021-12-01T13:54:35Z2021SILVA, Bruno Jorge de Andrade et al. Autophagy-associated IL-15 production is involved in the pathogenesis of leprosy type 1 reaction. Cells, v. 10, n. 9, p. 1-20, 27 Aug. 2021.2073-4409https://www.arca.fiocruz.br/handle/icict/5012610.3390/cells100922152073-4409engMDPIAutofagiaMycobacterium lepraeHanseníase ou T1RMacrófago ou THP-1FagossomoLisossomosIL-15AutophagyMycobacterium lepraeLeprosy or T1RMacrophage or THP-1PhagosomeLysosomesIL-15Autophagy-associated IL-15 production is involved in the pathogenesis of leprosy type 1 reactioninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Estrutural. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil.Leprosy reactional episodes are acute inflammatory events that may occur during the clinical course of the disease. Type 1 reaction (T1R) is associated with an increase in neural damage, and the understanding of the molecular pathways related to T1R onset is pivotal for the development of strategies that may effectively control the reaction. Interferon-gamma (IFN-γ) is a key cytokine associated with T1R onset and is also associated with autophagy induction. Here, we evaluated the modulation of the autophagy pathway in Mycobacterium leprae-stimulated cells in the presence or absence of IFN-γ. We observed that IFN-γ treatment promoted autophagy activation and increased the expression of genes related to the formation of phagosomes, autophagy regulation and function, or lysosomal pathways in M. leprae-stimulated cells. IFN-γ increased interleukin (IL)-15 secretion in M. leprae-stimulated THP-1 cells in a process associated with autophagy activation. We also observed higher IL15 gene expression in multibacillary (MB) patients who later developed T1R during clinical follow-up when compared to MB patients who did not develop the episode. By overlapping gene expression patterns, we observed 13 common elements shared between T1R skin lesion cells and THP-1 cells stimulated with both M. leprae and IFN-γ. Among these genes, the autophagy regulator Translocated Promoter Region, Nuclear Basket Protein (TPR) was significantly increased in T1R cells when compared with non-reactional MB cells. Overall, our results indicate that IFN-γ may induce a TPR-mediated autophagy transcriptional program in M. leprae-stimulated cells similar to that observed in skin cells during T1R by a pathway that involves IL-15 production, suggesting the involvement of this cytokine in the pathogenesis of T1R.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/50126/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALSuzanaCorteReal_EusenirNunes_etal_IOC_2021.pdfSuzanaCorteReal_EusenirNunes_etal_IOC_2021.pdfapplication/pdf8722350https://www.arca.fiocruz.br/bitstream/icict/50126/2/SuzanaCorteReal_EusenirNunes_etal_IOC_2021.pdf8e1a11bf6c21eb0251b351db19ec35b5MD52icict/501262023-08-02 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| dc.title.en.fl_str_mv |
Autophagy-associated IL-15 production is involved in the pathogenesis of leprosy type 1 reaction |
| title |
Autophagy-associated IL-15 production is involved in the pathogenesis of leprosy type 1 reaction |
| spellingShingle |
Autophagy-associated IL-15 production is involved in the pathogenesis of leprosy type 1 reaction Silva, Bruno Jorge de Andrade Autofagia Mycobacterium leprae Hanseníase ou T1R Macrófago ou THP-1 Fagossomo Lisossomos IL-15 Autophagy Mycobacterium leprae Leprosy or T1R Macrophage or THP-1 Phagosome Lysosomes IL-15 |
| title_short |
Autophagy-associated IL-15 production is involved in the pathogenesis of leprosy type 1 reaction |
| title_full |
Autophagy-associated IL-15 production is involved in the pathogenesis of leprosy type 1 reaction |
| title_fullStr |
Autophagy-associated IL-15 production is involved in the pathogenesis of leprosy type 1 reaction |
| title_full_unstemmed |
Autophagy-associated IL-15 production is involved in the pathogenesis of leprosy type 1 reaction |
| title_sort |
Autophagy-associated IL-15 production is involved in the pathogenesis of leprosy type 1 reaction |
| author |
Silva, Bruno Jorge de Andrade |
| author_facet |
Silva, Bruno Jorge de Andrade Bittencourt, Tamiris Lameira Leal-Calvo, Thyago Mendes, Mayara Abud Prata, Rhana Berto da Silva Barbosa, Mayara Garcia de Mattos Andrade, Priscila Ribeiro Côrte-Real, Suzana Silva, Gilberto Marcelo Sperandio da Moraes, Milton Osório Sarno, Euzenir Nunes Pinheiro, Roberta Olmo |
| author_role |
author |
| author2 |
Bittencourt, Tamiris Lameira Leal-Calvo, Thyago Mendes, Mayara Abud Prata, Rhana Berto da Silva Barbosa, Mayara Garcia de Mattos Andrade, Priscila Ribeiro Côrte-Real, Suzana Silva, Gilberto Marcelo Sperandio da Moraes, Milton Osório Sarno, Euzenir Nunes Pinheiro, Roberta Olmo |
| author2_role |
author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Silva, Bruno Jorge de Andrade Bittencourt, Tamiris Lameira Leal-Calvo, Thyago Mendes, Mayara Abud Prata, Rhana Berto da Silva Barbosa, Mayara Garcia de Mattos Andrade, Priscila Ribeiro Côrte-Real, Suzana Silva, Gilberto Marcelo Sperandio da Moraes, Milton Osório Sarno, Euzenir Nunes Pinheiro, Roberta Olmo |
| dc.subject.other.pt_BR.fl_str_mv |
Autofagia Mycobacterium leprae Hanseníase ou T1R Macrófago ou THP-1 Fagossomo Lisossomos IL-15 |
| topic |
Autofagia Mycobacterium leprae Hanseníase ou T1R Macrófago ou THP-1 Fagossomo Lisossomos IL-15 Autophagy Mycobacterium leprae Leprosy or T1R Macrophage or THP-1 Phagosome Lysosomes IL-15 |
| dc.subject.en.en.fl_str_mv |
Autophagy Mycobacterium leprae Leprosy or T1R Macrophage or THP-1 Phagosome Lysosomes |
| dc.subject.en.pt_BR.fl_str_mv |
IL-15 |
| description |
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil. |
| publishDate |
2021 |
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2021-12-01T13:54:35Z |
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2021-12-01T13:54:35Z |
| dc.date.issued.fl_str_mv |
2021 |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
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SILVA, Bruno Jorge de Andrade et al. Autophagy-associated IL-15 production is involved in the pathogenesis of leprosy type 1 reaction. Cells, v. 10, n. 9, p. 1-20, 27 Aug. 2021. |
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https://www.arca.fiocruz.br/handle/icict/50126 |
| dc.identifier.issn.pt_BR.fl_str_mv |
2073-4409 |
| dc.identifier.doi.pt_BR.fl_str_mv |
10.3390/cells10092215 |
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2073-4409 |
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SILVA, Bruno Jorge de Andrade et al. Autophagy-associated IL-15 production is involved in the pathogenesis of leprosy type 1 reaction. Cells, v. 10, n. 9, p. 1-20, 27 Aug. 2021. 2073-4409 10.3390/cells10092215 |
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