Comprehensive Quantitative Proteome Analysis of Aedes aegypti Identifies Proteins and Pathways Involved in Wolbachia pipientis and Zika Virus Interference Phenomenon

Detalhes bibliográficos
Autor(a) principal: Martins, Michele
Data de Publicação: 2021
Outros Autores: Ramos, Luis Felipe Costa, Murillo, Jimmy Rodriguez, Torres, André, Carvalho, Stephanie Serafim de, Domont, Gilberto Barbosa, Oliveira, Danielle Maria Perpétua de, Mesquita, Rafael Dias, Nogueira, Fábio César Sousa, Freitas, Rafael Maciel de, Junqueira, Magno
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/50923
Resumo: Universidade Federal do Rio de Janeiro. Instituto de Química. Departamento de Bioquímica. Rio de Janeiro, RJ, Brasil.
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spelling Martins, MicheleRamos, Luis Felipe CostaMurillo, Jimmy RodriguezTorres, AndréCarvalho, Stephanie Serafim deDomont, Gilberto BarbosaOliveira, Danielle Maria Perpétua deMesquita, Rafael DiasNogueira, Fábio César SousaFreitas, Rafael Maciel deJunqueira, Magno2022-01-27T18:51:24Z2022-01-27T18:51:24Z2021MARTINS, MIchele et al. Comprehensive Quantitative Proteome Analysis of Aedes aegypti Identifies Proteins and Pathways Involved in Wolbachia pipientis and Zika Virus Interference Phenomenon. Frontiers in Physiology, v. 12, Article 642237, p. 1 - 19, Feb. 2021.1664-042Xhttps://www.arca.fiocruz.br/handle/icict/5092310.3389/fphys.2021.642237engFrontiers MediaAedes aegyptiZika vírusQuantitativoWolbachia pipientisProteomaResposta imuneProteomeQuantitativeAedes aegyptiWolbachia pipientisZika virusImmune responseComprehensive Quantitative Proteome Analysis of Aedes aegypti Identifies Proteins and Pathways Involved in Wolbachia pipientis and Zika Virus Interference Phenomenoninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal do Rio de Janeiro. Instituto de Química. Departamento de Bioquímica. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Química. Departamento de Bioquímica. Rio de Janeiro, RJ, Brasil.Division of Chemistry I, Department of Medical Biochemistry and Biophysics, Karolinska Institute, Stockholm, Sweden.Universidade Federal do Rio de Janeiro. Instituto de Biofisica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Química. Departamento de Bioquímica. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Química. Departamento de Bioquímica. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Química. Departamento de Bioquímica. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Química. Departamento de Bioquímica. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Química. Departamento de Bioquímica. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Mosquitos Transmissores de Hematozoários. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Química. Departamento de Bioquímica. Rio de Janeiro, RJ, Brasil.Zika virus (ZIKV) is a global public health emergency due to its association with microcephaly, Guillain-Barré syndrome, neuropathy, and myelitis in children and adults. A total of 87 countries have had evidence of autochthonous mosquito-borne transmission of ZIKV, distributed across four continents, and no antivirus therapy or vaccines are available. Therefore, several strategies have been developed to target the main mosquito vector, Aedes aegypti, to reduce the burden of different arboviruses. Among such strategies, the use of the maternally-inherited endosymbiont Wolbachia pipientis has been applied successfully to reduce virus susceptibility and decrease transmission. However, the mechanisms by which Wolbachia orchestrate resistance to ZIKV infection remain to be elucidated. In this study, we apply isobaric labeling quantitative mass spectrometry (MS)-based proteomics to quantify proteins and identify pathways altered during ZIKV infection; Wolbachia infection; co-infection with Wolbachia/ZIKV in the A. aegypti heads and salivary glands. We show that Wolbachia regulates proteins involved in reactive oxygen species production, regulates humoral immune response, and antioxidant production. The reduction of ZIKV polyprotein in the presence of Wolbachia in mosquitoes was determined by MS and corroborates the idea that Wolbachia helps to block ZIKV infections in A. aegypti. The present study offers a rich resource of data that may help to elucidate mechanisms by which Wolbachia orchestrate resistance to ZIKV infection in A. aegypti, and represents a step further on the development of new targeted methods to detect and quantify ZIKV and Wolbachia directly in complex tissues.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/50923/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALRafaelMacielde_Freitas_etal_IOC_2021.pdfRafaelMacielde_Freitas_etal_IOC_2021.pdfapplication/pdf2819129https://www.arca.fiocruz.br/bitstream/icict/50923/2/RafaelMacielde_Freitas_etal_IOC_2021.pdf79d6acf1702b36ba8300d5e8d7bc6326MD52icict/509232022-01-27 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dc.title.pt_BR.fl_str_mv Comprehensive Quantitative Proteome Analysis of Aedes aegypti Identifies Proteins and Pathways Involved in Wolbachia pipientis and Zika Virus Interference Phenomenon
title Comprehensive Quantitative Proteome Analysis of Aedes aegypti Identifies Proteins and Pathways Involved in Wolbachia pipientis and Zika Virus Interference Phenomenon
spellingShingle Comprehensive Quantitative Proteome Analysis of Aedes aegypti Identifies Proteins and Pathways Involved in Wolbachia pipientis and Zika Virus Interference Phenomenon
Martins, Michele
Aedes aegypti
Zika vírus
Quantitativo
Wolbachia pipientis
Proteoma
Resposta imune
Proteome
Quantitative
Aedes aegypti
Wolbachia pipientis
Zika virus
Immune response
title_short Comprehensive Quantitative Proteome Analysis of Aedes aegypti Identifies Proteins and Pathways Involved in Wolbachia pipientis and Zika Virus Interference Phenomenon
title_full Comprehensive Quantitative Proteome Analysis of Aedes aegypti Identifies Proteins and Pathways Involved in Wolbachia pipientis and Zika Virus Interference Phenomenon
title_fullStr Comprehensive Quantitative Proteome Analysis of Aedes aegypti Identifies Proteins and Pathways Involved in Wolbachia pipientis and Zika Virus Interference Phenomenon
title_full_unstemmed Comprehensive Quantitative Proteome Analysis of Aedes aegypti Identifies Proteins and Pathways Involved in Wolbachia pipientis and Zika Virus Interference Phenomenon
title_sort Comprehensive Quantitative Proteome Analysis of Aedes aegypti Identifies Proteins and Pathways Involved in Wolbachia pipientis and Zika Virus Interference Phenomenon
author Martins, Michele
author_facet Martins, Michele
Ramos, Luis Felipe Costa
Murillo, Jimmy Rodriguez
Torres, André
Carvalho, Stephanie Serafim de
Domont, Gilberto Barbosa
Oliveira, Danielle Maria Perpétua de
Mesquita, Rafael Dias
Nogueira, Fábio César Sousa
Freitas, Rafael Maciel de
Junqueira, Magno
author_role author
author2 Ramos, Luis Felipe Costa
Murillo, Jimmy Rodriguez
Torres, André
Carvalho, Stephanie Serafim de
Domont, Gilberto Barbosa
Oliveira, Danielle Maria Perpétua de
Mesquita, Rafael Dias
Nogueira, Fábio César Sousa
Freitas, Rafael Maciel de
Junqueira, Magno
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Martins, Michele
Ramos, Luis Felipe Costa
Murillo, Jimmy Rodriguez
Torres, André
Carvalho, Stephanie Serafim de
Domont, Gilberto Barbosa
Oliveira, Danielle Maria Perpétua de
Mesquita, Rafael Dias
Nogueira, Fábio César Sousa
Freitas, Rafael Maciel de
Junqueira, Magno
dc.subject.other.pt_BR.fl_str_mv Aedes aegypti
Zika vírus
Quantitativo
Wolbachia pipientis
Proteoma
Resposta imune
topic Aedes aegypti
Zika vírus
Quantitativo
Wolbachia pipientis
Proteoma
Resposta imune
Proteome
Quantitative
Aedes aegypti
Wolbachia pipientis
Zika virus
Immune response
dc.subject.en.pt_BR.fl_str_mv Proteome
Quantitative
Aedes aegypti
Wolbachia pipientis
Zika virus
Immune response
description Universidade Federal do Rio de Janeiro. Instituto de Química. Departamento de Bioquímica. Rio de Janeiro, RJ, Brasil.
publishDate 2021
dc.date.issued.fl_str_mv 2021
dc.date.accessioned.fl_str_mv 2022-01-27T18:51:24Z
dc.date.available.fl_str_mv 2022-01-27T18:51:24Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv MARTINS, MIchele et al. Comprehensive Quantitative Proteome Analysis of Aedes aegypti Identifies Proteins and Pathways Involved in Wolbachia pipientis and Zika Virus Interference Phenomenon. Frontiers in Physiology, v. 12, Article 642237, p. 1 - 19, Feb. 2021.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/50923
dc.identifier.issn.pt_BR.fl_str_mv 1664-042X
dc.identifier.doi.none.fl_str_mv 10.3389/fphys.2021.642237
identifier_str_mv MARTINS, MIchele et al. Comprehensive Quantitative Proteome Analysis of Aedes aegypti Identifies Proteins and Pathways Involved in Wolbachia pipientis and Zika Virus Interference Phenomenon. Frontiers in Physiology, v. 12, Article 642237, p. 1 - 19, Feb. 2021.
1664-042X
10.3389/fphys.2021.642237
url https://www.arca.fiocruz.br/handle/icict/50923
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
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reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/50923/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/50923/2/RafaelMacielde_Freitas_etal_IOC_2021.pdf
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