Techniques for the detection of pathogenic Cryptococcus species in wood decay substrata and the evaluation of viability in stored samples

Detalhes bibliográficos
Autor(a) principal: Alvarez, Christian
Data de Publicação: 2013
Outros Autores: Barbosa, Glaucia Gonçalves, Oliveira, Raquel de Vasconcellos Carvalhaes de, Morales, Bernardina Penarrieta, Wanke, Bodo, Lazéra, Márcia dos Santos
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/35770
Resumo: Hospital del Niño Jesús. Laboratorio de Micología. Tucumán, Argentina.
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spelling Alvarez, ChristianBarbosa, Glaucia GonçalvesOliveira, Raquel de Vasconcellos Carvalhaes deMorales, Bernardina PenarrietaWanke, BodoLazéra, Márcia dos Santos2019-09-23T12:01:19Z2019-09-23T12:01:19Z2013ALVAREZ, Christian et al. Techniques for the detection of pathogenic Cryptococcus species in wood decay substrata and the evaluation of viability in stored samples. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 108, n. 1, p. 126-129, Feb. 2013.0074-0276https://www.arca.fiocruz.br/handle/icict/3577010.1590/S0074-027620130001000231678-8060engInstituto Oswaldo Cruz, Ministério da SaúdeTechniques for the detection of pathogenic Cryptococcus species in wood decay substrata and the evaluation of viability in stored samplesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleHospital del Niño Jesús. Laboratorio de Micología. Tucumán, Argentina.Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil.In this study, we evaluated several techniques for the detection of the yeast form of Cryptococcus in decaying wood and measured the viability of these fungi in environmental samples stored in the laboratory. Samples were collected from a tree known to be positive for Cryptococcus and were each inoculated on 10 Niger seed agar (NSA) plates. The conventional technique (CT) yielded a greater number of positive samples and indicated a higher fungal density [in colony forming units per gram of wood (CFU.g-1)] compared to the humid swab technique (ST). However, the difference in positive and false negative results between the CT-ST was not significant. The threshold of detection for the CT was 0.05.103 CFU.g-1, while the threshold for the ST was greater than 0.1.103 CFU-1. No colonies were recovered using the dry swab technique. We also determined the viability of Cryptococcus in wood samples stored for 45 days at 25ºC using the CT and ST and found that samples not only continued to yield a positive response, but also exhibited an increase in CFU.g-1, suggesting that Cryptococcus is able to grow in stored environmental samples. The ST.1, in which samples collected with swabs were immediately plated on NSA medium, was more efficient and less laborious than either the CT or ST and required approximately 10 min to perform; however, additional studies are needed to validate this technique.Isolation techniquesCryptococcus neoformansCryptococcus gattiiWood decay substratainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txttext/plain1748https://www.arca.fiocruz.br/bitstream/icict/35770/1/license.txt8a4605be74aa9ea9d79846c1fba20a33MD51ORIGINALve_Alvarez_Christian_etal_INI_2013.pdfve_Alvarez_Christian_etal_INI_2013.pdfapplication/pdf280454https://www.arca.fiocruz.br/bitstream/icict/35770/2/ve_Alvarez_Christian_etal_INI_2013.pdf5b85ca3c17be36983e2975c8836176a3MD52TEXTve_Alvarez_Christian_etal_INI_2013.pdf.txtve_Alvarez_Christian_etal_INI_2013.pdf.txtExtracted texttext/plain19238https://www.arca.fiocruz.br/bitstream/icict/35770/3/ve_Alvarez_Christian_etal_INI_2013.pdf.txte546341a7223e64a7eb006f0add033cdMD53icict/357702019-09-24 02:03:28.792oai:www.arca.fiocruz.br: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Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352019-09-24T05:03:28Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Techniques for the detection of pathogenic Cryptococcus species in wood decay substrata and the evaluation of viability in stored samples
title Techniques for the detection of pathogenic Cryptococcus species in wood decay substrata and the evaluation of viability in stored samples
spellingShingle Techniques for the detection of pathogenic Cryptococcus species in wood decay substrata and the evaluation of viability in stored samples
Alvarez, Christian
Isolation techniques
Cryptococcus neoformans
Cryptococcus gattii
Wood decay substrata
title_short Techniques for the detection of pathogenic Cryptococcus species in wood decay substrata and the evaluation of viability in stored samples
title_full Techniques for the detection of pathogenic Cryptococcus species in wood decay substrata and the evaluation of viability in stored samples
title_fullStr Techniques for the detection of pathogenic Cryptococcus species in wood decay substrata and the evaluation of viability in stored samples
title_full_unstemmed Techniques for the detection of pathogenic Cryptococcus species in wood decay substrata and the evaluation of viability in stored samples
title_sort Techniques for the detection of pathogenic Cryptococcus species in wood decay substrata and the evaluation of viability in stored samples
author Alvarez, Christian
author_facet Alvarez, Christian
Barbosa, Glaucia Gonçalves
Oliveira, Raquel de Vasconcellos Carvalhaes de
Morales, Bernardina Penarrieta
Wanke, Bodo
Lazéra, Márcia dos Santos
author_role author
author2 Barbosa, Glaucia Gonçalves
Oliveira, Raquel de Vasconcellos Carvalhaes de
Morales, Bernardina Penarrieta
Wanke, Bodo
Lazéra, Márcia dos Santos
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Alvarez, Christian
Barbosa, Glaucia Gonçalves
Oliveira, Raquel de Vasconcellos Carvalhaes de
Morales, Bernardina Penarrieta
Wanke, Bodo
Lazéra, Márcia dos Santos
dc.subject.en.pt_BR.fl_str_mv Isolation techniques
Cryptococcus neoformans
Cryptococcus gattii
Wood decay substrata
topic Isolation techniques
Cryptococcus neoformans
Cryptococcus gattii
Wood decay substrata
description Hospital del Niño Jesús. Laboratorio de Micología. Tucumán, Argentina.
publishDate 2013
dc.date.issued.fl_str_mv 2013
dc.date.accessioned.fl_str_mv 2019-09-23T12:01:19Z
dc.date.available.fl_str_mv 2019-09-23T12:01:19Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv ALVAREZ, Christian et al. Techniques for the detection of pathogenic Cryptococcus species in wood decay substrata and the evaluation of viability in stored samples. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 108, n. 1, p. 126-129, Feb. 2013.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/35770
dc.identifier.issn.pt_BR.fl_str_mv 0074-0276
dc.identifier.doi.none.fl_str_mv 10.1590/S0074-02762013000100023
dc.identifier.eissn.none.fl_str_mv 1678-8060
identifier_str_mv ALVAREZ, Christian et al. Techniques for the detection of pathogenic Cryptococcus species in wood decay substrata and the evaluation of viability in stored samples. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 108, n. 1, p. 126-129, Feb. 2013.
0074-0276
10.1590/S0074-02762013000100023
1678-8060
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