Multifunction CD4+T cells in patients with American cutaneous leishmanioses

Detalhes bibliográficos
Autor(a) principal: Macedo, A. B. B.
Data de Publicação: 2011
Outros Autores: Sánchez-Arcila, J. C., Schubach, A. O., Mendonça, S.C.F., Marins-dos-Santos, A., Madeira, M. de Fátima, Gagini, T., Pimentel, M. I. F., De Luca, P. M.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/36977
Resumo: 2020-11-11
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spelling Macedo, A. B. B.Sánchez-Arcila, J. C.Schubach, A. O.Mendonça, S.C.F.Marins-dos-Santos, A.Madeira, M. de FátimaGagini, T.Pimentel, M. I. F.De Luca, P. M.2019-11-11T14:14:26Z2019-11-11T14:14:26Z2011MACEDO, A. B. B. et al. Multifunctional CD4+ T cells in patients with American cutaneous leishmaniasis. Clinical and Experimental Immunology, v. 167, p. 505-513, 2011.0009-9104https://www.arca.fiocruz.br/handle/icict/3697710.1111/j.1365-2249.2011.04536.x1365-2249engWileyMultifunction CD4+T cells in patients with American cutaneous leishmaniosesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article2020-11-11Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunoparasitologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunoparasitologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Laboratório de Vigilância em Leishmanioses. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunoparasitologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Plataforma de Citometria de Fluxo. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Laboratório de Vigilância em Leishmanioses. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunoparasitologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Laboratório de Vigilância em Leishmanioses. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunoparasitologia. Rio de Janeiro, RJ, Brasil.Leishmaniasis is a group of important parasitic diseases affecting millions worldwide. To understand more clearly the quality of T helper type 1 (Th1) response stimulated after Leishmania infection, we applied a multiparametric flow cytometry protocol to evaluate multifunctional T cells induced by crude antigen extracts obtained from promastigotes of Leishmania braziliensis (LbAg) and Leishmania amazonensis (LaAg) in peripheral blood mononuclear cells from healed cutaneous leishmaniasis patients. Although no significant difference was detected in the percentage of total interferon (IFN)-γ-producing CD4+ T cells induced by both antigens, multiparametric flow cytometry analysis revealed clear differences in the quality of Th1 responses. LbAg induced an important proportion of multifunctional CD4+T cells (28% of the total Th1 response evaluated), whereas LaAg induced predominantly single-positive cells (68%), and 57% of those were IFN-γ single-positives. Multifunctional CD4+ T cells showed the highest mean fluorescence intensity (MFI) for the three Th1 cytokines assessed and MFIs for IFN-γ and interleukin-2 from those cells stimulated with LbAg were significantly higher than those obtained after LaAg stimulation. These major differences observed in the generation of multifunctional CD4+ T cells suggest that the quality of the Th1 response induced by L. amazonensis antigens can be involved in the mechanisms responsible for the high susceptibility observed in L. amazonensis-infected individuals. Ultimately, our results call attention to the importance of studying a Th1 response regarding its quality, not just its magnitude, and indicate that this kind of evaluation might help understanding of the complex and diverse immunopathogenesis of American tegumentary leishmaniasis.Human American cutaneous leishmaniasisLeishmania amazonensisLeishmania braziliensisMultifunctional T cellsinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txttext/plain1748https://www.arca.fiocruz.br/bitstream/icict/36977/1/license.txt8a4605be74aa9ea9d79846c1fba20a33MD51ORIGINALve_Macedo_A_etal_INI_2011.pdfve_Macedo_A_etal_INI_2011.pdfapplication/pdf301945https://www.arca.fiocruz.br/bitstream/icict/36977/2/ve_Macedo_A_etal_INI_2011.pdf61e56a612a9bcc698ee03e206f884b7cMD52TEXTve_Macedo_A_etal_INI_2011.pdf.txtve_Macedo_A_etal_INI_2011.pdf.txtExtracted texttext/plain47216https://www.arca.fiocruz.br/bitstream/icict/36977/3/ve_Macedo_A_etal_INI_2011.pdf.txt9394c2f950bdf5066eeaa868248d6d8aMD53icict/369772021-03-24 16:32:38.77oai:www.arca.fiocruz.br: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Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352021-03-24T19:32:38Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Multifunction CD4+T cells in patients with American cutaneous leishmanioses
title Multifunction CD4+T cells in patients with American cutaneous leishmanioses
spellingShingle Multifunction CD4+T cells in patients with American cutaneous leishmanioses
Macedo, A. B. B.
Human American cutaneous leishmaniasis
Leishmania amazonensis
Leishmania braziliensis
Multifunctional T cells
title_short Multifunction CD4+T cells in patients with American cutaneous leishmanioses
title_full Multifunction CD4+T cells in patients with American cutaneous leishmanioses
title_fullStr Multifunction CD4+T cells in patients with American cutaneous leishmanioses
title_full_unstemmed Multifunction CD4+T cells in patients with American cutaneous leishmanioses
title_sort Multifunction CD4+T cells in patients with American cutaneous leishmanioses
author Macedo, A. B. B.
author_facet Macedo, A. B. B.
Sánchez-Arcila, J. C.
Schubach, A. O.
Mendonça, S.C.F.
Marins-dos-Santos, A.
Madeira, M. de Fátima
Gagini, T.
Pimentel, M. I. F.
De Luca, P. M.
author_role author
author2 Sánchez-Arcila, J. C.
Schubach, A. O.
Mendonça, S.C.F.
Marins-dos-Santos, A.
Madeira, M. de Fátima
Gagini, T.
Pimentel, M. I. F.
De Luca, P. M.
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Macedo, A. B. B.
Sánchez-Arcila, J. C.
Schubach, A. O.
Mendonça, S.C.F.
Marins-dos-Santos, A.
Madeira, M. de Fátima
Gagini, T.
Pimentel, M. I. F.
De Luca, P. M.
dc.subject.en.pt_BR.fl_str_mv Human American cutaneous leishmaniasis
Leishmania amazonensis
Leishmania braziliensis
Multifunctional T cells
topic Human American cutaneous leishmaniasis
Leishmania amazonensis
Leishmania braziliensis
Multifunctional T cells
description 2020-11-11
publishDate 2011
dc.date.issued.fl_str_mv 2011
dc.date.accessioned.fl_str_mv 2019-11-11T14:14:26Z
dc.date.available.fl_str_mv 2019-11-11T14:14:26Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv MACEDO, A. B. B. et al. Multifunctional CD4+ T cells in patients with American cutaneous leishmaniasis. Clinical and Experimental Immunology, v. 167, p. 505-513, 2011.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/36977
dc.identifier.issn.pt_BR.fl_str_mv 0009-9104
dc.identifier.doi.none.fl_str_mv 10.1111/j.1365-2249.2011.04536.x
dc.identifier.eissn.none.fl_str_mv 1365-2249
identifier_str_mv MACEDO, A. B. B. et al. Multifunctional CD4+ T cells in patients with American cutaneous leishmaniasis. Clinical and Experimental Immunology, v. 167, p. 505-513, 2011.
0009-9104
10.1111/j.1365-2249.2011.04536.x
1365-2249
url https://www.arca.fiocruz.br/handle/icict/36977
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
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