GC-MS Profile and Enhancement of Antibiotic Activity by the Essential Oil of Ocotea odorífera and Safrole: Inhibition of Staphylococcus aureus Efflux Pumps
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2020 |
| Outros Autores: | , , , , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/42024 |
Resumo: | Regional University of Cariri-URCA. Department of Biological Chemistry. Crato, CE, Brazil. |
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Almeida, Ray S.Freitas, Priscilla RamosAraújo, Ana Carolina JustinoMenezes, Irwin R. A.Santos, Eduardo L.Tintino, Saulo R.Moura, Talysson F.Filho, Jaime R.Ferreira, Vitória A.Silva, Ana Cristina A.Silva, Luiz E.Amaral, Wanderlei doDeschamps, CíceroIriti, MarcelloCoutinho, Henrique D. M.2020-07-01T14:08:12Z2020-07-01T14:08:12Z2020ALMEIDA, Ray S. et al. GC-MS Profile and Enhancement of Antibiotic Activity by th Essential Oil of Ocotea odorífera and Safrole: Inhibition of Staphylococcus aureus Efflux Pumps. Antibiotics, v. 9, p. 247, 2020.2079-6382https://www.arca.fiocruz.br/handle/icict/4202410.3390/antibiotics9050247engMDPIBrometo de etídioResistência bacterianaAntibioticsEthidium bromideBacterial resistanceBiological activityChemical compositionGC-MS Profile and Enhancement of Antibiotic Activity by the Essential Oil of Ocotea odorífera and Safrole: Inhibition of Staphylococcus aureus Efflux Pumpsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleRegional University of Cariri-URCA. Department of Biological Chemistry. Crato, CE, Brazil.Regional University of Cariri-URCA. Department of Biological Chemistry. Crato, CE, Brazil.Regional University of Cariri-URCA. Department of Biological Chemistry. Crato, CE, Brazil.Regional University of Cariri-URCA. Department of Biological Chemistry. Crato, CE, Brazil.Regional University of Cariri-URCA. Department of Biological Chemistry. Crato, CE, Brazil.Regional University of Cariri-URCA. Department of Biological Chemistry. Crato, CE, Brazil.Regional University of Cariri-URCA. Department of Biological Chemistry. Crato, CE, Brazil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.Christus University Center-Unichristus. Department of Biomedicine. Fortaleza, CE, Brazil.Maurício de Nassau College UNINASSAU. Department of Biomedicine. Petrolina, PE, Brazil.Federal University of Paraná. Post Graduate Program in Sustainable Territorial Development. Matinhos, PR, Brazil.Federal University of Paraná. Post Graduate Program in Sustainable Territorial Development. Matinhos, PR, Brazil.Federal University of Paraná. Department of Chemistry. Post Graduate Program in Agronomy. Curitiba, PR, Brazil.Milan State University. Department of Agricultural and Environmental Sciences. Milan, Italy.Regional University of Cariri-URCA. Department of Biological Chemistry. Crato, CE, Brazil.Considering the evidence that essential oils, as well as safrole, could modulate bacterial growth in different resistant strains, this study aims to characterize the phytochemical profile and evaluate the antibacterial and antibiotic-modulating properties of the essential oil Ocotea odorífera (EOOO) and safrole against efflux pump (EP)-carrying strains. The EOOO was extracted by hydrodistillation, and the phytochemical analysis was performed by gas chromatography coupled to mass spectrometry (GC-MS). The antibacterial and antibiotic-modulating activities of the EOOO and safrole against resistant strains of Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa were analyzed through the broth microdilution method. The EP-inhibiting potential of safrole in association with ethidium bromide or antibiotics was evaluated using the S. aureus 1199B and K2068 strains, which carry genes encoding efflux proteins associated with antibiotic resistance to norfloxacin and ciprofloxacin, respectively. A reduction in the MIC of ethidium bromide or antibiotics was used as a parameter of EP inhibition. The phytochemical analysis identified 16 different compounds in the EOOO including safrole as the principal constituent. While the EOOO and safrole exerted clinically relevant antibacterial effects against S. aureus only, they potentiated the antibacterial activity of norfloxacin against all strains evaluated by our study. The ethidium bromide and antibiotic assays using the strains of S. aureus SA1119B and K2068, as well as molecular docking analysis, indicated that safrole inhibits the NorA and MepA efflux pumps in S. aureus. In conclusion, Ocotea odorifera and safrole presented promising antibacterial and antibiotic-enhancing properties, which should be explored in the development of drugs to combat antibacterial resistance, especially in strains bearing genes encoding efflux proteins.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/42024/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALAlmeida RS GC-MS profile....pdfAlmeida RS GC-MS profile....pdfapplication/pdf2862299https://www.arca.fiocruz.br/bitstream/icict/42024/2/Almeida%20RS%20%20GC-MS%20profile....pdf950825d6a8d89211bdf47d754d188d02MD52TEXTAlmeida RS GC-MS profile....pdf.txtAlmeida RS GC-MS profile....pdf.txtExtracted 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| dc.title.pt_BR.fl_str_mv |
GC-MS Profile and Enhancement of Antibiotic Activity by the Essential Oil of Ocotea odorífera and Safrole: Inhibition of Staphylococcus aureus Efflux Pumps |
| title |
GC-MS Profile and Enhancement of Antibiotic Activity by the Essential Oil of Ocotea odorífera and Safrole: Inhibition of Staphylococcus aureus Efflux Pumps |
| spellingShingle |
GC-MS Profile and Enhancement of Antibiotic Activity by the Essential Oil of Ocotea odorífera and Safrole: Inhibition of Staphylococcus aureus Efflux Pumps Almeida, Ray S. Brometo de etídio Resistência bacteriana Antibiotics Ethidium bromide Bacterial resistance Biological activity Chemical composition |
| title_short |
GC-MS Profile and Enhancement of Antibiotic Activity by the Essential Oil of Ocotea odorífera and Safrole: Inhibition of Staphylococcus aureus Efflux Pumps |
| title_full |
GC-MS Profile and Enhancement of Antibiotic Activity by the Essential Oil of Ocotea odorífera and Safrole: Inhibition of Staphylococcus aureus Efflux Pumps |
| title_fullStr |
GC-MS Profile and Enhancement of Antibiotic Activity by the Essential Oil of Ocotea odorífera and Safrole: Inhibition of Staphylococcus aureus Efflux Pumps |
| title_full_unstemmed |
GC-MS Profile and Enhancement of Antibiotic Activity by the Essential Oil of Ocotea odorífera and Safrole: Inhibition of Staphylococcus aureus Efflux Pumps |
| title_sort |
GC-MS Profile and Enhancement of Antibiotic Activity by the Essential Oil of Ocotea odorífera and Safrole: Inhibition of Staphylococcus aureus Efflux Pumps |
| author |
Almeida, Ray S. |
| author_facet |
Almeida, Ray S. Freitas, Priscilla Ramos Araújo, Ana Carolina Justino Menezes, Irwin R. A. Santos, Eduardo L. Tintino, Saulo R. Moura, Talysson F. Filho, Jaime R. Ferreira, Vitória A. Silva, Ana Cristina A. Silva, Luiz E. Amaral, Wanderlei do Deschamps, Cícero Iriti, Marcello Coutinho, Henrique D. M. |
| author_role |
author |
| author2 |
Freitas, Priscilla Ramos Araújo, Ana Carolina Justino Menezes, Irwin R. A. Santos, Eduardo L. Tintino, Saulo R. Moura, Talysson F. Filho, Jaime R. Ferreira, Vitória A. Silva, Ana Cristina A. Silva, Luiz E. Amaral, Wanderlei do Deschamps, Cícero Iriti, Marcello Coutinho, Henrique D. M. |
| author2_role |
author author author author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Almeida, Ray S. Freitas, Priscilla Ramos Araújo, Ana Carolina Justino Menezes, Irwin R. A. Santos, Eduardo L. Tintino, Saulo R. Moura, Talysson F. Filho, Jaime R. Ferreira, Vitória A. Silva, Ana Cristina A. Silva, Luiz E. Amaral, Wanderlei do Deschamps, Cícero Iriti, Marcello Coutinho, Henrique D. M. |
| dc.subject.other.pt_BR.fl_str_mv |
Brometo de etídio Resistência bacteriana Antibiotics |
| topic |
Brometo de etídio Resistência bacteriana Antibiotics Ethidium bromide Bacterial resistance Biological activity Chemical composition |
| dc.subject.en.pt_BR.fl_str_mv |
Ethidium bromide Bacterial resistance Biological activity Chemical composition |
| description |
Regional University of Cariri-URCA. Department of Biological Chemistry. Crato, CE, Brazil. |
| publishDate |
2020 |
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2020-07-01T14:08:12Z |
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2020-07-01T14:08:12Z |
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2020 |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
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ALMEIDA, Ray S. et al. GC-MS Profile and Enhancement of Antibiotic Activity by th Essential Oil of Ocotea odorífera and Safrole: Inhibition of Staphylococcus aureus Efflux Pumps. Antibiotics, v. 9, p. 247, 2020. |
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https://www.arca.fiocruz.br/handle/icict/42024 |
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2079-6382 |
| dc.identifier.doi.none.fl_str_mv |
10.3390/antibiotics9050247 |
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ALMEIDA, Ray S. et al. GC-MS Profile and Enhancement of Antibiotic Activity by th Essential Oil of Ocotea odorífera and Safrole: Inhibition of Staphylococcus aureus Efflux Pumps. Antibiotics, v. 9, p. 247, 2020. 2079-6382 10.3390/antibiotics9050247 |
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https://www.arca.fiocruz.br/handle/icict/42024 |
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MDPI |
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MDPI |
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