Extraction of Trypanosoma cruzi DNA from food: a contribution to the elucidation of acute Chagas disease outbreaks

Detalhes bibliográficos
Autor(a) principal: Ferreira, Renata Trotta Barroso
Data de Publicação: 2016
Outros Autores: Melandre, Aline Martins, Cabral, Maria Luiza, Branquinho, Maria Regina, Leite, Paola Cardarelli
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/14568
http://dx.doi.org/10.1590/0037-8682-0414-2015
Resumo: Fundação Oswaldo Cruz. Instituto Nacional de Controle de Qualidade em Saúde. Departamento de Microbiologia. Rio de Janeiro, RJ, Brasil.
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spelling Ferreira, Renata Trotta BarrosoMelandre, Aline MartinsCabral, Maria LuizaBranquinho, Maria ReginaLeite, Paola Cardarelli2016-06-21T16:24:58Z2016-07-07T11:32:46Z2016-06-21T16:24:58Z2016-07-07T11:32:46Z2016FERREIRA, R. T. B. et al. Extraction of Trypanosoma cruzi DNA from food: a contribution to the elucidation of acute Chagas disease outbreaks. Rev. Soc. Bras. Med. Trop., Uberaba, v. 49, n. 2, p. 190-195, 2016.0037-8682https://www.arca.fiocruz.br/handle/icict/14568http://dx.doi.org/10.1590/0037-8682-0414-20151678-9849engSociedade Brasileira de Medicina TropicalDoença de ChagasTrypanosoma cruziAçaíDetecção de PCRAnálise de AlimentosChagas DiseaseTrypanosoma cruziEuterpePCR detectionFood analysisDoença de ChagasTrypanosoma cruziEuterpeReação em Cadeia da PolimeraseAnálise de AlimentosExtraction of Trypanosoma cruzi DNA from food: a contribution to the elucidation of acute Chagas disease outbreaksinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Nacional de Controle de Qualidade em Saúde. Departamento de Microbiologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Controle de Qualidade em Saúde. Departamento de Microbiologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Controle de Qualidade em Saúde. Departamento de Microbiologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Controle de Qualidade em Saúde. Departamento de Microbiologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Controle de Qualidade em Saúde. Departamento de Microbiologia. Rio de Janeiro, RJ, Brasil.INTRODUCTION: Before 2004, the occurrence of acute Chagas disease (ACD) by oral transmission associated with food was scarcely known or investigated. Originally sporadic and circumstantial, ACD occurrences have now become frequent in the Amazon region, with recently related outbreaks spreading to several Brazilian states. These cases are associated with the consumption of açai juice by waste reservoir animals or insect vectors infected with Trypanosoma cruzi in endemic areas. Although guidelines for processing the fruit to minimize contamination through microorganisms and parasites exist, açai-based products must be assessed for quality, for which the demand for appropriate methodologies must be met. METHODS: Dilutions ranging from 5 to 1,000 T. cruzi CL Brener cells were mixed with 2mL of acai juice. Four Extraction of T. cruzi DNA methods were used on the fruit, and the cetyltrimethyl ammonium bromide (CTAB) method was selected according to JRC, 2005. RESULTS: DNA extraction by the CTAB method yielded satisfactory results with regard to purity and concentration for use in PCR. Overall, the methods employed proved that not only extraction efficiency but also high sensitivity in amplification was important. CONCLUSIONS: The method for T. cruzi detection in food is a powerful tool in the epidemiological investigation of outbreaks as it turns epidemiological evidence into supporting data that serve to confirm T. cruzi infection in the foods. It also facilitates food quality control and assessment of good manufacturing practices involving acai-based products.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZTEXTRev_Soc_Bras_Med_Trop_49_190-195.pdf.txtRev_Soc_Bras_Med_Trop_49_190-195.pdf.txtExtracted texttext/plain29441https://www.arca.fiocruz.br/bitstream/icict/14568/4/Rev_Soc_Bras_Med_Trop_49_190-195.pdf.txt937b2da2a68d3b28dcde96d9e65cad38MD54ORIGINALRev_Soc_Bras_Med_Trop_49_190-195.pdfapplication/pdf1221967https://www.arca.fiocruz.br/bitstream/icict/14568/2/Rev_Soc_Bras_Med_Trop_49_190-195.pdf9203763b2f56a5988e93457ba6acfaa2MD52LICENSElicense.txttext/plain2991https://www.arca.fiocruz.br/bitstream/icict/14568/3/license.txt5a560609d32a3863062d77ff32785d58MD53icict/145682018-04-02 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dc.title.en.fl_str_mv Extraction of Trypanosoma cruzi DNA from food: a contribution to the elucidation of acute Chagas disease outbreaks
title Extraction of Trypanosoma cruzi DNA from food: a contribution to the elucidation of acute Chagas disease outbreaks
spellingShingle Extraction of Trypanosoma cruzi DNA from food: a contribution to the elucidation of acute Chagas disease outbreaks
Ferreira, Renata Trotta Barroso
Doença de Chagas
Trypanosoma cruzi
Açaí
Detecção de PCR
Análise de Alimentos
Chagas Disease
Trypanosoma cruzi
Euterpe
PCR detection
Food analysis
Doença de Chagas
Trypanosoma cruzi
Euterpe
Reação em Cadeia da Polimerase
Análise de Alimentos
title_short Extraction of Trypanosoma cruzi DNA from food: a contribution to the elucidation of acute Chagas disease outbreaks
title_full Extraction of Trypanosoma cruzi DNA from food: a contribution to the elucidation of acute Chagas disease outbreaks
title_fullStr Extraction of Trypanosoma cruzi DNA from food: a contribution to the elucidation of acute Chagas disease outbreaks
title_full_unstemmed Extraction of Trypanosoma cruzi DNA from food: a contribution to the elucidation of acute Chagas disease outbreaks
title_sort Extraction of Trypanosoma cruzi DNA from food: a contribution to the elucidation of acute Chagas disease outbreaks
author Ferreira, Renata Trotta Barroso
author_facet Ferreira, Renata Trotta Barroso
Melandre, Aline Martins
Cabral, Maria Luiza
Branquinho, Maria Regina
Leite, Paola Cardarelli
author_role author
author2 Melandre, Aline Martins
Cabral, Maria Luiza
Branquinho, Maria Regina
Leite, Paola Cardarelli
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Ferreira, Renata Trotta Barroso
Melandre, Aline Martins
Cabral, Maria Luiza
Branquinho, Maria Regina
Leite, Paola Cardarelli
dc.subject.other.pt_BR.fl_str_mv Doença de Chagas
Trypanosoma cruzi
Açaí
Detecção de PCR
Análise de Alimentos
topic Doença de Chagas
Trypanosoma cruzi
Açaí
Detecção de PCR
Análise de Alimentos
Chagas Disease
Trypanosoma cruzi
Euterpe
PCR detection
Food analysis
Doença de Chagas
Trypanosoma cruzi
Euterpe
Reação em Cadeia da Polimerase
Análise de Alimentos
dc.subject.en.en.fl_str_mv Chagas Disease
Trypanosoma cruzi
Euterpe
PCR detection
Food analysis
dc.subject.decs.pt_BR.fl_str_mv Doença de Chagas
Trypanosoma cruzi
Euterpe
Reação em Cadeia da Polimerase
Análise de Alimentos
description Fundação Oswaldo Cruz. Instituto Nacional de Controle de Qualidade em Saúde. Departamento de Microbiologia. Rio de Janeiro, RJ, Brasil.
publishDate 2016
dc.date.accessioned.fl_str_mv 2016-06-21T16:24:58Z
2016-07-07T11:32:46Z
dc.date.available.fl_str_mv 2016-06-21T16:24:58Z
2016-07-07T11:32:46Z
dc.date.issued.fl_str_mv 2016
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.citation.fl_str_mv FERREIRA, R. T. B. et al. Extraction of Trypanosoma cruzi DNA from food: a contribution to the elucidation of acute Chagas disease outbreaks. Rev. Soc. Bras. Med. Trop., Uberaba, v. 49, n. 2, p. 190-195, 2016.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/14568
dc.identifier.issn.pt_BR.fl_str_mv 0037-8682
dc.identifier.doi.pt_BR.fl_str_mv http://dx.doi.org/10.1590/0037-8682-0414-2015
dc.identifier.eissn.pt_BR.fl_str_mv 1678-9849
identifier_str_mv FERREIRA, R. T. B. et al. Extraction of Trypanosoma cruzi DNA from food: a contribution to the elucidation of acute Chagas disease outbreaks. Rev. Soc. Bras. Med. Trop., Uberaba, v. 49, n. 2, p. 190-195, 2016.
0037-8682
1678-9849
url https://www.arca.fiocruz.br/handle/icict/14568
http://dx.doi.org/10.1590/0037-8682-0414-2015
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Sociedade Brasileira de Medicina Tropical
publisher.none.fl_str_mv Sociedade Brasileira de Medicina Tropical
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
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