Culture of mouse peritoneal macrophages with mouse serum induces lipid bodies that associate with the parasitophorous vacuole and decrease their microbicidal capacity against Toxoplasma gondii

Detalhes bibliográficos
Autor(a) principal: Mota, Laura Azeredo Miranda
Data de Publicação: 2014
Outros Autores: Roberto Neto, João, Monteiro, Verônica Gomes, Lobato, Caroliny Samary Silva, Oliveira, Marco Antonio de, Cunha, Maura da, D'Avila, Heloisa, Seabra, Sérgio Henrique, Bozza, Patricia Torres, DaMatta, Renato Augusto
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/10554
Resumo: Universidade Estadual do Norte Fluminense. Laboratório de Biologia Celular e Tecidual. Campos dos Goytacases, RJ, Brasil.
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spelling Mota, Laura Azeredo MirandaRoberto Neto, JoãoMonteiro, Verônica GomesLobato, Caroliny Samary SilvaOliveira, Marco Antonio deCunha, Maura daD'Avila, HeloisaSeabra, Sérgio HenriqueBozza, Patricia TorresDaMatta, Renato Augusto2015-05-27T13:39:52Z2015-05-27T13:39:52Z2014MOTA, Laura Azeredo et al. Culture of mouse peritoneal macrophages with mouse serum induces lipid bodies that associate with the parasitophorous vacuole and decrease their microbicidal capacity against Toxoplasma gondii. Mem Inst Oswaldo Cruz, v.109, n.6, p.767-774, Sept. 2014.1678-8060https://www.arca.fiocruz.br/handle/icict/1055410.1590/0074-0276140119engFundação Oswaldo CruzCulture of mouse peritoneal macrophages with mouse serum induces lipid bodies that associate with the parasitophorous vacuole and decrease their microbicidal capacity against Toxoplasma gondiiinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Estadual do Norte Fluminense. Laboratório de Biologia Celular e Tecidual. Campos dos Goytacases, RJ, Brasil.Universidade Estadual do Norte Fluminense. Laboratório de Biologia Celular e Tecidual. Campos dos Goytacases, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil.Universidade Estadual do Norte Fluminense. Laboratório de Biologia Celular e Tecidual. Campos dos Goytacases, RJ, Brasil.Universidade Estadual do Norte Fluminense. Laboratório de Biologia Celular e Tecidual. Campos dos Goytacases, RJ, Brasil.Universidade Estadual do Norte Fluminense. Laboratório de Biologia Celular e Tecidual. Campos dos Goytacases, RJ, Brasil / Universidade do Vale do Paraíba. Instituto de Pesquisa e Desenvolvimento. Laboratório de Parasitologia e Biotecnologia. São José dos Campos, SP, Brasil.Universidade Estadual do Norte Fluminense. Laboratório de Biologia Celular e Tecidual. Campos dos Goytacases, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil / Universidade Federal de Juiz de Fora. Laboratório de Biologia Celular. Juiz de Fora, MG, Brasil.Centro Universitário Estadual da Zona Oeste. Laboratório de Tecnologia em Bioquímica e Microscopia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil.Universidade Estadual do Norte Fluminense. Laboratório de Biologia Celular e Tecidual. Campos dos Goytacases, RJ, Brasil.Lipid bodies [lipid droplets (LBs)] are lipid-rich organelles involved in lipid metabolism, signalling and inflammation. Recent findings suggest a role for LBs in host response to infection; however, the potential functions of this organelle in Toxoplasma gondii infection and how it alters macrophage microbicidal capacity during infection are not well understood. Here, we investigated the role of host LBs in T. gondii infection in mouse peritoneal macrophages in vitro. Macrophages cultured with mouse serum (MS) had higher numbers of LBs than those cultured in foetal bovine serum and can function as a model to study the role of LBs during intracellular pathogen infection. LBs were found in association with the parasitophorous vacuole, suggesting that T. gondii may benefit from this lipid source. Moreover, increased numbers of macrophage LBs correlated with high prostaglandin E2 (PGE2) production and decreased nitric oxide (NO) synthesis. Accordingly, LB-enriched macrophages cultured with MS were less efficient at controlling T. gondii growth. Treatment of macrophages cultured with MS with indomethacin, an inhibitor of PGE2 production, increased the microbicidal capacity against T. gondii. Collectively, these results suggest that culture with MS caused a decrease in microbicidal activity of macrophages against T. gondii by increasing PGE2 while lowering NO production.MacrophagesLipid bodiesNitric oxideÓxido NítricoMicrobicidal capacityToxoplasma gondiiToxoplasmaMacrófagosÓxido NítricoProstaglandinasinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txttext/plain1914https://www.arca.fiocruz.br/bitstream/icict/10554/1/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD51ORIGINALpatricia_bozzaetal_IOC_014.pdfapplication/pdf1773946https://www.arca.fiocruz.br/bitstream/icict/10554/2/patricia_bozzaetal_IOC_014.pdf7d7ed4921cbc12370091c28c2cde838aMD52TEXTpatricia_bozzaetal_IOC_014.pdf.txtpatricia_bozzaetal_IOC_014.pdf.txtExtracted texttext/plain37105https://www.arca.fiocruz.br/bitstream/icict/10554/3/patricia_bozzaetal_IOC_014.pdf.txt71439456415924e8ea2dde0a2e36ee59MD53icict/105542022-06-24 13:08:49.841oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352022-06-24T16:08:49Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Culture of mouse peritoneal macrophages with mouse serum induces lipid bodies that associate with the parasitophorous vacuole and decrease their microbicidal capacity against Toxoplasma gondii
title Culture of mouse peritoneal macrophages with mouse serum induces lipid bodies that associate with the parasitophorous vacuole and decrease their microbicidal capacity against Toxoplasma gondii
spellingShingle Culture of mouse peritoneal macrophages with mouse serum induces lipid bodies that associate with the parasitophorous vacuole and decrease their microbicidal capacity against Toxoplasma gondii
Mota, Laura Azeredo Miranda
Macrophages
Lipid bodies
Nitric oxide
Óxido Nítrico
Microbicidal capacity
Toxoplasma gondii
Toxoplasma
Macrófagos
Óxido Nítrico
Prostaglandinas
title_short Culture of mouse peritoneal macrophages with mouse serum induces lipid bodies that associate with the parasitophorous vacuole and decrease their microbicidal capacity against Toxoplasma gondii
title_full Culture of mouse peritoneal macrophages with mouse serum induces lipid bodies that associate with the parasitophorous vacuole and decrease their microbicidal capacity against Toxoplasma gondii
title_fullStr Culture of mouse peritoneal macrophages with mouse serum induces lipid bodies that associate with the parasitophorous vacuole and decrease their microbicidal capacity against Toxoplasma gondii
title_full_unstemmed Culture of mouse peritoneal macrophages with mouse serum induces lipid bodies that associate with the parasitophorous vacuole and decrease their microbicidal capacity against Toxoplasma gondii
title_sort Culture of mouse peritoneal macrophages with mouse serum induces lipid bodies that associate with the parasitophorous vacuole and decrease their microbicidal capacity against Toxoplasma gondii
author Mota, Laura Azeredo Miranda
author_facet Mota, Laura Azeredo Miranda
Roberto Neto, João
Monteiro, Verônica Gomes
Lobato, Caroliny Samary Silva
Oliveira, Marco Antonio de
Cunha, Maura da
D'Avila, Heloisa
Seabra, Sérgio Henrique
Bozza, Patricia Torres
DaMatta, Renato Augusto
author_role author
author2 Roberto Neto, João
Monteiro, Verônica Gomes
Lobato, Caroliny Samary Silva
Oliveira, Marco Antonio de
Cunha, Maura da
D'Avila, Heloisa
Seabra, Sérgio Henrique
Bozza, Patricia Torres
DaMatta, Renato Augusto
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Mota, Laura Azeredo Miranda
Roberto Neto, João
Monteiro, Verônica Gomes
Lobato, Caroliny Samary Silva
Oliveira, Marco Antonio de
Cunha, Maura da
D'Avila, Heloisa
Seabra, Sérgio Henrique
Bozza, Patricia Torres
DaMatta, Renato Augusto
dc.subject.en.pt_BR.fl_str_mv Macrophages
Lipid bodies
Nitric oxide
Óxido Nítrico
Microbicidal capacity
Toxoplasma gondii
topic Macrophages
Lipid bodies
Nitric oxide
Óxido Nítrico
Microbicidal capacity
Toxoplasma gondii
Toxoplasma
Macrófagos
Óxido Nítrico
Prostaglandinas
dc.subject.decs.pt_BR.fl_str_mv Toxoplasma
Macrófagos
Óxido Nítrico
Prostaglandinas
description Universidade Estadual do Norte Fluminense. Laboratório de Biologia Celular e Tecidual. Campos dos Goytacases, RJ, Brasil.
publishDate 2014
dc.date.issued.fl_str_mv 2014
dc.date.accessioned.fl_str_mv 2015-05-27T13:39:52Z
dc.date.available.fl_str_mv 2015-05-27T13:39:52Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv MOTA, Laura Azeredo et al. Culture of mouse peritoneal macrophages with mouse serum induces lipid bodies that associate with the parasitophorous vacuole and decrease their microbicidal capacity against Toxoplasma gondii. Mem Inst Oswaldo Cruz, v.109, n.6, p.767-774, Sept. 2014.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/10554
dc.identifier.issn.pt_BR.fl_str_mv 1678-8060
dc.identifier.doi.pt_BR.fl_str_mv 10.1590/0074-0276140119
identifier_str_mv MOTA, Laura Azeredo et al. Culture of mouse peritoneal macrophages with mouse serum induces lipid bodies that associate with the parasitophorous vacuole and decrease their microbicidal capacity against Toxoplasma gondii. Mem Inst Oswaldo Cruz, v.109, n.6, p.767-774, Sept. 2014.
1678-8060
10.1590/0074-0276140119
url https://www.arca.fiocruz.br/handle/icict/10554
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Fundação Oswaldo Cruz
publisher.none.fl_str_mv Fundação Oswaldo Cruz
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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https://www.arca.fiocruz.br/bitstream/icict/10554/2/patricia_bozzaetal_IOC_014.pdf
https://www.arca.fiocruz.br/bitstream/icict/10554/3/patricia_bozzaetal_IOC_014.pdf.txt
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