Intranasal Immunization with Pressure Inactivated Avian Influenza Elicits Cellular and Humoral Responses in Mice

Detalhes bibliográficos
Autor(a) principal: Barroso, Shana P. C.
Data de Publicação: 2015
Outros Autores: Nico, Dirlei, Nascimento, Danielle, Santos, Ana Clara V., Couceiro, José Nelson S. S., Bozza, Fernando A., Ferreira, Ana M. A., Ferreira, Davis F., Sousa, Clarisa B. Palatnik de, Souza, Thiago Moreno L., Gomes, Andre M. O., Silva, Jerson L., Oliveira, Andréa C.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/13731
Resumo: Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica Leopoldo de Meis. Rio de Janeiro, RJ, Brasil / Instituto Nacional de Ciência e Tecnologia de Biologia Estrutural e Bioimagem. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Vírus Respiratórios, WHO/NIC. Rio de Janeiro, RJ, Brasil.
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spelling Barroso, Shana P. C.Nico, DirleiNascimento, DanielleSantos, Ana Clara V.Couceiro, José Nelson S. S.Bozza, Fernando A.Ferreira, Ana M. A.Ferreira, Davis F.Sousa, Clarisa B. Palatnik deSouza, Thiago Moreno L.Gomes, Andre M. O.Silva, Jerson L.Oliveira, Andréa C.2016-04-12T16:34:52Z2016-04-12T16:34:52Z2015BARROSO, Shana P. C. et al. Intranasal Immunization with Pressure Inactivated Avian Influenza Elicits Cellular and Humoral Responses in Mice. Plos One, v. 10, n. 6, p. 1-20, Jun. 2015.1932-6203https://www.arca.fiocruz.br/handle/icict/1373110.1371/journal.pone.0128785engPublic Library of ScienceIntranasal Immunization with Pressure Inactivated Avian Influenza Elicits Cellular and Humoral Responses in Miceinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica Leopoldo de Meis. Rio de Janeiro, RJ, Brasil / Instituto Nacional de Ciência e Tecnologia de Biologia Estrutural e Bioimagem. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Vírus Respiratórios, WHO/NIC. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo Góes. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica Leopoldo de Meis. Rio de Janeiro, RJ, Brasil / Instituto Nacional de Ciência e Tecnologia de Biologia Estrutural e Bioimagem. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo Góes. Rio de Janeiro, RJ, Brasil.Instituto Nacional de Ciência e Tecnologia de Biologia Estrutural e Bioimagem. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Rio de Janeiro, RJ, BrasilUniversidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo Góes. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo Góes. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo Góes. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Vírus Respiratórios, WHO/NIC. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica Leopoldo de Meis. Rio de Janeiro, RJ, Brasil / Instituto Nacional de Ciência e Tecnologia de Biologia Estrutural e Bioimagem. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica Leopoldo de Meis. Rio de Janeiro, RJ, Brasil / Instituto Nacional de Ciência e Tecnologia de Biologia Estrutural e Bioimagem. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica Leopoldo de Meis. Rio de Janeiro, RJ, Brasil / Instituto Nacional de Ciência e Tecnologia de Biologia Estrutural e Bioimagem. Rio de Janeiro, RJ, Brasil.Influenza viruses pose a serious global health threat, particularly in light of newly emerging strains, such as the avian influenza H5N1 and H7N9 viruses. Vaccination remains the primary method for preventing acquiring influenza or for avoiding developing serious complications related to the disease. Vaccinations based on inactivated split virus vaccines or on chemically inactivated whole virus have some important drawbacks, including changes in the immunogenic properties of the virus. To induce a greater mucosal immune response, intranasally administered vaccines are highly desired as they not only prevent disease but can also block the infection at its primary site. To avoid these drawbacks, hydrostatic pressure has been used as a potential method for viral inactivation and vaccine production. In this study, we show that hydrostatic pressure inactivates the avian influenza A H3N8 virus, while still maintaining hemagglutinin and neuraminidase functionalities. Challenged vaccinated animals showed no disease signs (ruffled fur, lethargy, weight loss, and huddling). Similarly, these animals showed less Evans Blue dye leakage and lower cell counts in their bronchoalveolar lavage fluid compared with the challenged non-vaccinated group. We found that the whole inactivated particles were capable of generating a neutralizing antibody response in serum, and IgA was also found in nasal mucosa and feces. After the vaccination and challenge we observed Th1/Th2 cytokine secretion with a prevalence of IFN-γ. Our data indicate that the animals present a satisfactory immune response after vaccination and are protected against infection. Our results may pave the way for the development of a novel pressure-based vaccine against influenza virus.Influenza virusesIntranasal ImmunizationVacinationMiceAvian influenzaOrthomyxoviridaeImunizaçãoVacinaçãoCamundongosInfluenza Aviáriainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/13731/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALthiago_souza_etal_IOC_2015.PDFthiago_souza_etal_IOC_2015.PDFapplication/pdf2315372https://www.arca.fiocruz.br/bitstream/icict/13731/2/thiago_souza_etal_IOC_2015.PDF5c1bb08c1de53f70772b0e6d29a9bf7cMD52TEXTthiago_souza_etal_IOC_2015.PDF.txtthiago_souza_etal_IOC_2015.PDF.txtExtracted texttext/plain69914https://www.arca.fiocruz.br/bitstream/icict/13731/3/thiago_souza_etal_IOC_2015.PDF.txtb5215a3f5edf3407c204c77830867801MD53icict/137312023-09-05 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dc.title.pt_BR.fl_str_mv Intranasal Immunization with Pressure Inactivated Avian Influenza Elicits Cellular and Humoral Responses in Mice
title Intranasal Immunization with Pressure Inactivated Avian Influenza Elicits Cellular and Humoral Responses in Mice
spellingShingle Intranasal Immunization with Pressure Inactivated Avian Influenza Elicits Cellular and Humoral Responses in Mice
Barroso, Shana P. C.
Influenza viruses
Intranasal Immunization
Vacination
Mice
Avian influenza
Orthomyxoviridae
Imunização
Vacinação
Camundongos
Influenza Aviária
title_short Intranasal Immunization with Pressure Inactivated Avian Influenza Elicits Cellular and Humoral Responses in Mice
title_full Intranasal Immunization with Pressure Inactivated Avian Influenza Elicits Cellular and Humoral Responses in Mice
title_fullStr Intranasal Immunization with Pressure Inactivated Avian Influenza Elicits Cellular and Humoral Responses in Mice
title_full_unstemmed Intranasal Immunization with Pressure Inactivated Avian Influenza Elicits Cellular and Humoral Responses in Mice
title_sort Intranasal Immunization with Pressure Inactivated Avian Influenza Elicits Cellular and Humoral Responses in Mice
author Barroso, Shana P. C.
author_facet Barroso, Shana P. C.
Nico, Dirlei
Nascimento, Danielle
Santos, Ana Clara V.
Couceiro, José Nelson S. S.
Bozza, Fernando A.
Ferreira, Ana M. A.
Ferreira, Davis F.
Sousa, Clarisa B. Palatnik de
Souza, Thiago Moreno L.
Gomes, Andre M. O.
Silva, Jerson L.
Oliveira, Andréa C.
author_role author
author2 Nico, Dirlei
Nascimento, Danielle
Santos, Ana Clara V.
Couceiro, José Nelson S. S.
Bozza, Fernando A.
Ferreira, Ana M. A.
Ferreira, Davis F.
Sousa, Clarisa B. Palatnik de
Souza, Thiago Moreno L.
Gomes, Andre M. O.
Silva, Jerson L.
Oliveira, Andréa C.
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Barroso, Shana P. C.
Nico, Dirlei
Nascimento, Danielle
Santos, Ana Clara V.
Couceiro, José Nelson S. S.
Bozza, Fernando A.
Ferreira, Ana M. A.
Ferreira, Davis F.
Sousa, Clarisa B. Palatnik de
Souza, Thiago Moreno L.
Gomes, Andre M. O.
Silva, Jerson L.
Oliveira, Andréa C.
dc.subject.en.pt_BR.fl_str_mv Influenza viruses
Intranasal Immunization
Vacination
Mice
Avian influenza
topic Influenza viruses
Intranasal Immunization
Vacination
Mice
Avian influenza
Orthomyxoviridae
Imunização
Vacinação
Camundongos
Influenza Aviária
dc.subject.decs.pt_BR.fl_str_mv Orthomyxoviridae
Imunização
Vacinação
Camundongos
Influenza Aviária
description Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica Leopoldo de Meis. Rio de Janeiro, RJ, Brasil / Instituto Nacional de Ciência e Tecnologia de Biologia Estrutural e Bioimagem. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Vírus Respiratórios, WHO/NIC. Rio de Janeiro, RJ, Brasil.
publishDate 2015
dc.date.issued.fl_str_mv 2015
dc.date.accessioned.fl_str_mv 2016-04-12T16:34:52Z
dc.date.available.fl_str_mv 2016-04-12T16:34:52Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv BARROSO, Shana P. C. et al. Intranasal Immunization with Pressure Inactivated Avian Influenza Elicits Cellular and Humoral Responses in Mice. Plos One, v. 10, n. 6, p. 1-20, Jun. 2015.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/13731
dc.identifier.issn.none.fl_str_mv 1932-6203
dc.identifier.doi.none.fl_str_mv 10.1371/journal.pone.0128785
identifier_str_mv BARROSO, Shana P. C. et al. Intranasal Immunization with Pressure Inactivated Avian Influenza Elicits Cellular and Humoral Responses in Mice. Plos One, v. 10, n. 6, p. 1-20, Jun. 2015.
1932-6203
10.1371/journal.pone.0128785
url https://www.arca.fiocruz.br/handle/icict/13731
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
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collection Repositório Institucional da FIOCRUZ (ARCA)
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