Evaluation of parasitological and molecular techniques for the diagnosis and assessment of cure of schistosomiasis mansoni in a low transmission area
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2015 |
| Outros Autores: | , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/14485 |
Resumo: | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Esquistossomose. Belo Horizonte, MG, Brasil. |
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Siqueira, Liliane Maria VidalGomes, Luciana InáciaOliveira, Edward José deOliveira, Eduardo Ribeiro deOliveira, Áureo Almeida deEnk, Martin JohannesCarneiro, Nídia FigueiredoRabello, Ana Lucia TelesCoelho, Paulo Marcos Zech2016-06-16T17:21:47Z2016-07-08T02:09:53Z2016-06-16T17:21:47Z2016-07-08T02:09:53Z2015SIQUEIRA, Liliane Maria Vidal et al. Evaluation of parasitological and molecular techniques for the diagnosis and assessment of cure of schistosomiasis mansoni in a low transmission area. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 110, n. 2, p. 209-214, 2015.0074-0276https://www.arca.fiocruz.br/handle/icict/1448510.1590/0074-02760140375engFundação Oswaldo CruzEvaluation of parasitological and molecular techniques for the diagnosis and assessment of cure of schistosomiasis mansoni in a low transmission areainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Esquistossomose. Belo Horizonte, MG, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Pesquisas Clínicas. Belo Horizonte, MG, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Pesquisas Clínicas. Belo Horizonte, MG, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Pesquisas Clínicas. Belo Horizonte, MG, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Esquistossomose. Belo Horizonte, MG, Brasil.Instituto Evandro Chagas. Ananindeua, PA, Brasil.Prefeitura de Montes Claros. Centro de Controle de Zoonoses. Montes Claros, MG, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Pesquisas Clínicas. Belo Horizonte, MG, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Esquistossomose. Belo Horizonte, MG, Brasil.This study evaluated parasitological and molecular techniques for the diagnosis and assessment of cure of schistosomiasis mansoni. A population-based study was performed in 201 inhabitants from a low transmission locality named Pedra Preta, municipality of Montes Claros, state of Minas Gerais, Brazil. Four stool samples were analysed using two techniques, the Kato-Katz® (KK) technique (18 slides) and the TF-Test®, to establish the infection rate. The positivity rate of 18 KK slides of four stool samples was 28.9% (58/201) and the combined parasitological techniques (KK+TF-Test®) produced a 35.8% positivity rate (72/201). Furthermore, a polymerase chain reaction (PCR)-ELISA assay produced a positivity rate of 23.4% (47/201) using the first sample. All 72 patients with positive parasitological exams were treated with a single dose of Praziquantel® and these patients were followed-up 30, 90 and 180 days after treatment to establish the cure rate. Cure rates obtained by the analysis of 12 KK slides were 100%, 100% and 98.4% at 30, 90 and 180 days after treatment, respectively. PCR-ELISA revealed cure rates of 98.5%, 95.5% and 96.5%, respectively. The diagnostic and assessment of cure for schistosomiasis may require an increased number of KK slides or a test with higher sensitivity, such as PCR-ELISA, in situations of very low parasite load, such as after therapeutic interventions.Schistosoma mansoniSchistosomiasisDiagnosisLow transmission areaControlinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZTEXTve_Liliane_Siqueira_etal_Evaluation_CPqRR_2015.pdf.txtve_Liliane_Siqueira_etal_Evaluation_CPqRR_2015.pdf.txtExtracted 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| dc.title.pt_BR.fl_str_mv |
Evaluation of parasitological and molecular techniques for the diagnosis and assessment of cure of schistosomiasis mansoni in a low transmission area |
| title |
Evaluation of parasitological and molecular techniques for the diagnosis and assessment of cure of schistosomiasis mansoni in a low transmission area |
| spellingShingle |
Evaluation of parasitological and molecular techniques for the diagnosis and assessment of cure of schistosomiasis mansoni in a low transmission area Siqueira, Liliane Maria Vidal Schistosoma mansoni Schistosomiasis Diagnosis Low transmission area Control |
| title_short |
Evaluation of parasitological and molecular techniques for the diagnosis and assessment of cure of schistosomiasis mansoni in a low transmission area |
| title_full |
Evaluation of parasitological and molecular techniques for the diagnosis and assessment of cure of schistosomiasis mansoni in a low transmission area |
| title_fullStr |
Evaluation of parasitological and molecular techniques for the diagnosis and assessment of cure of schistosomiasis mansoni in a low transmission area |
| title_full_unstemmed |
Evaluation of parasitological and molecular techniques for the diagnosis and assessment of cure of schistosomiasis mansoni in a low transmission area |
| title_sort |
Evaluation of parasitological and molecular techniques for the diagnosis and assessment of cure of schistosomiasis mansoni in a low transmission area |
| author |
Siqueira, Liliane Maria Vidal |
| author_facet |
Siqueira, Liliane Maria Vidal Gomes, Luciana Inácia Oliveira, Edward José de Oliveira, Eduardo Ribeiro de Oliveira, Áureo Almeida de Enk, Martin Johannes Carneiro, Nídia Figueiredo Rabello, Ana Lucia Teles Coelho, Paulo Marcos Zech |
| author_role |
author |
| author2 |
Gomes, Luciana Inácia Oliveira, Edward José de Oliveira, Eduardo Ribeiro de Oliveira, Áureo Almeida de Enk, Martin Johannes Carneiro, Nídia Figueiredo Rabello, Ana Lucia Teles Coelho, Paulo Marcos Zech |
| author2_role |
author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Siqueira, Liliane Maria Vidal Gomes, Luciana Inácia Oliveira, Edward José de Oliveira, Eduardo Ribeiro de Oliveira, Áureo Almeida de Enk, Martin Johannes Carneiro, Nídia Figueiredo Rabello, Ana Lucia Teles Coelho, Paulo Marcos Zech |
| dc.subject.en.pt_BR.fl_str_mv |
Schistosoma mansoni Schistosomiasis Diagnosis Low transmission area Control |
| topic |
Schistosoma mansoni Schistosomiasis Diagnosis Low transmission area Control |
| description |
Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Esquistossomose. Belo Horizonte, MG, Brasil. |
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2015 |
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2015 |
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2016-06-16T17:21:47Z 2016-07-08T02:09:53Z |
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2016-06-16T17:21:47Z 2016-07-08T02:09:53Z |
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SIQUEIRA, Liliane Maria Vidal et al. Evaluation of parasitological and molecular techniques for the diagnosis and assessment of cure of schistosomiasis mansoni in a low transmission area. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 110, n. 2, p. 209-214, 2015. |
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https://www.arca.fiocruz.br/handle/icict/14485 |
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0074-0276 |
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10.1590/0074-02760140375 |
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SIQUEIRA, Liliane Maria Vidal et al. Evaluation of parasitological and molecular techniques for the diagnosis and assessment of cure of schistosomiasis mansoni in a low transmission area. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 110, n. 2, p. 209-214, 2015. 0074-0276 10.1590/0074-02760140375 |
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